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| 1 | Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)显示文摘High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC4F2 and BC4F3 populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality. | Tao Guo Xiaolu Liu Xiangyuan Wan Jianfeng Weng Shijia Liu Xi Liu Mingjiang Chen Jingjing Li Ning Su Fuqing Wu Zhijun Cheng Xiuping Guo Cailin Lei Jiulin Wang Ling jiang Jianmin Wan | 2011 | Journal of Integrative Plant Biology2011，53，8： | 25 |
| 2 | A Novel Chloroplast-Localized Pentatricopeptide Repeat Protein Involved in Splicing Affects Chloroplast Development and Abiotic Stress Response in Rice显示文摘Pentatricopeptide 重复(PPR ) 蛋白质在更高的植物包括一个大家庭并且在 organellar RNA 新陈代谢的各种各样的方面调制 organellar 基因表示 byparticipating。在米饭,家庭包含他们的功能仍然是的 477 个成员,和 majorityof 不清楚。在这研究,我们孤立并且描绘米饭变异的、白条纹叶(wsl ) ,它在开发早的显示萎黄病有条纹或刻痕之状态。基于地图的克隆表明 WSL 编码指向叶绿体的最新识别的米饭 PPR 蛋白质。在 wsl 异种,锐气依赖者质体基因表示是显著地下面调整的,并且质体 rRNAs 和翻译 productsaccumulate 到很底层。一致地与观察, wsl 在叶绿体抄本 rpl2 拼接显示出一个强壮的缺点,在异种导致异常抄本累积和它的产品减小。wsl 显示出提高的敏感到骆驼毛的织物,咸度,和糖,并且它积累更多的 H 2 O 2 比野类型。这些结果建议减少的翻译效率可以影响异种的反应到 abioticstress.Key 词: | Junjie Tan Zhenhua Tan Fuqing Wu Peike Sheng Yueqin Heng Xinhua Wang Yulong Ren Jiulin Wang Xiuping Guo Xin Zhang Zhijun Cheng Ling Jiang Xuanming Liu Haiyang Wang Jianmin Wan | 2014 | Molecular Plant2014，7，8： | 15 |
| 3 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 4 | Transcriptional activation and phosphorylation of OsCNGC9 confer enhanced chilling tolerance in rice显示文摘Low temperature is a major environmental factor that limits plant growth and productivity.Although transient elevation of cytoplasmic calcium has long been recognized as a critical signal for plant cold tolerance,the calcium channels responsible for this process have remained largely elusive.Here we report that OsCNGC9,a cyclic nucleotide-gated channel,positively regulates chilling tolerance by mediating cytoplasmic calcium elevation in rice(Oryza sativa).We showed that the loss-of-function mutant of OsCNGC9 is defective in cold-induced calcium influx and more sensitive to prolonged cold treatment,whereas OsCNGC9 overexpression confers enhanced cold tolerance.Mechanistically,we demonstrated that in response to chilling stress,OsSAPK8,a homolog of Arabidopsis thaliana OST1,phosphorylates and activates OsCNGC9 to trigger Ca2+influx.Moreover,we found that the transcription of OsCNGC9 is activated by a rice dehydration-responsive element-binding transcription factor,OsDREB1A.Taken together,our results suggest that OsCNGC9 enhances chilling tolerance in rice through regulating cold-induced calcium influx and cytoplasmic calcium elevation. | Jiachang Wang Yulong Ren Xi Liu Sheng Luo Xiao Zhang Xin Liu Qibing Lin Shanshan Zhu Hua Wan Yang Yang Yu Zhang Bin Lei Chunlei Zhou Tian Pan Yongfei Wang Mingming Wu Ruonan jing Yang Xu Meng Han Fuqing Wu Cailin Lei Xiuping Guo Zhijun Cheng Xiaoming Zheng Yihua Wang Zhigang Zhao Ling Jiang Xin Zhang Yong-Fei Wang Haiyang Wang Jianmin Wan | 2021 | Molecular Plant2021，14，2： | 10 |
| 5 | Construction of a sustainable 3-hydroxybutyrate-producing probiotic Escherichia coli for treatment of colitis显示文摘Colitis is a common disease of the colon that is very difficult to treat.Probiotic bacteria could be an effective treatment.The probiotic Escherichia coli Nissle 1917(EcN)was engineered to synthesize the ketone body(R)-3-hydroxybutyrate(3HB)for sustainable production in the gut lumen of mice suffering from colitis.Components of heterologous 3HB synthesis routes were constructed,expressed,optimized,and inserted into the EcN genome,combined with deletions in competitive branch pathways.The genome-engineered EcN produced the highest 3HB level of 0.6 g/L under microaerobic conditions.The live therapeutic was found to colonize the mouse gastrointestinal tract over 14 days,elevating gut 3HB and short-chain-length fatty acid(SCFA)levels 8.7-and 3.1-fold compared to those of wild-type EcN,respectively.The sustainable presence of 3HB in mouse guts promoted the growth of probiotic bacteria,especially Akkermansia spp.,to over 31%from the initial 2%of all the microbiome.As a result,the engineered EcN termed EcNL4 ameliorated colitis induced via dextran sulfate sodium(DSS)in mice.Compared to wild-type EcN or oral administration of 3HB,oral EcNL4 uptake demonstrated better effects on mouse weights,colon lengths,occult blood levels,gut tissue myeloperoxidase activity and proinflammatory cytokine concentrations.Thus,a promising live bacterium was developed to improve colonic microenvironments and further treat colitis.This proof-of-concept design can be employed to treat other diseases of the colon. | Xu Yan Xin-Yi Liu Dian Zhang Yu-Dian Zhang Zi-Hua Li Xu Liu Fuqing Wu Guo-Qiang Chen | 2021 | Cellular & Molecular Immunology2021，18，10： | 8 |
| 6 | Loose architecture of multi-level massive geospatial data based on virtual quadtree显示文摘This paper proposed a virtual quadtree (VQT) based loose architecture of multi-level massive geospatial data for integrating massive geospatial data dispersed in the departments of different hierarchies in the same sector into a unified GIS (Geographic Information System) platform. By virtualizing the nodes of the quad-tree,the VQT separates the structure of data organization from data storage,and screens the difference between the data storage in local computer and in the re-mote computers in network environment. And by mounting,VQT easily integrates the data from the remote computers into the local VQT so as to implement seam-less integration of distributed multi-level massive geospatial data. Based on that mode,the paper built an application system with geospatial data over 1200 GB distributed in 12 servers deployed in 12 cities. The experiment showed that all data can be seamlessly rapidly traveled and performed zooming in and zooming out smoothly. | YANG ChongJun,WU Sheng,REN YingChao,FU Li,ZHANG FuQing,WANG Gang,TAN Jian,LIU DongLin,MA ChaoJi & LIANG Li State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China | 2008 | Science China(Technological Sciences)2008，51，S1： | 4 |
| 7 | Stable and realistic crack pattern generation using a cracking node method显示文摘 | Juan ZHANG Fuqing DUAN Mingquan ZHOU Dongcan JIANG Xuesong WANG Zhongke WU Youliang HUANG Guoguang DU Shaolong LIU Pengbo ZHOU Xiangang SHANG | 2018 | Frontiers of Computer Science2018，12，4： | 1 |
| 8 | Acupuncture and Moxibustion for Inflammatory Bowel Diseases: A Systematic Review and Meta-Analysis of Randomized Controlled Trials显示文摘 | Jun Ji Yuan Lu Huirong Liu Hui Feng Fuqing Zhang Luyi Wu Yunhua Cui Huangan Wu Yong-Qing Yang | 2013 | Evidence-Based Complementary and Alternative Medicine2013，，： | 1 |
| 9 | Propylene oxide-assisted fast sol-gel synthesis of mesoporous and nano-structured LiFePO4/C cathode materials显示文摘 | Wang Fuqing Chen Jian Wu Minghao Yi Baolian | 2013 | Ionics2013，19，3： | 1 |
| 10 | Propylene oxide-assisted fast sol–gel synthesis of mesoporous and nano-structured LiFePO 4 /C cathode materials显示文摘 | Fuqing Wang Jian Chen Minghao Wu Baolian Yi | 2013 | Ionics2013，，3： | 1 |
| 11 | Experiments on thermal fracture in rocks显示文摘The thermally induced fracturing appeared in the boundaries of mineral grains of rocks due to the different thermal expansion of different minerals while heating the rocks. When the microscopic fracturing connected like a network, the macroscopic permeability and the fluid transport properties of the rock changed remarkably. The implication of thermal fracturing to petroleum industry, earthquake process and transport modeling have been discussed. | Yong Chen Xiaodong Wu Fuqing Zhang | 1999 | Chinese Science Bulletin1999，44，17： | 1 |
| 12 | Geochemistry of petroleum systems in the eastern Pearl River Mouth Basin: evidence for mixed oils显示文摘 | Shuichang Zhang Digang Liang Zaisheng Gong Keqiang Wu Maowen Li Fuqing Song Zhiguang Song Dajiang Zhang Peirong Wang | 2003 | Organic Geochemistry2003，，： | 1 |
| 13 | A new linear algorithm for calibrating central eatadioptric cameras 显示文摘 | Wu Fuchao Duan Fuqing Hu Zhanyi | 2008 | Pattern Recognition2008，41，10： | 1 |
| 14 | The Mg-chelatase H subunit is an abscisic acid receptor显示文摘 | SHEN Yuanyue WANG Xiaofang WU Fuqing | 2006 | Nature2006，443，： | 1 |
| 15 | Construction of Smac gene-containing and human prostate specific antigen promoter-regulated vector and its expression显示文摘Objective: To construct an eukaryotic expression vector containing Smac gene and study the expression efficiency and speci- ficity of prostate specific antigen(PSA) enhancer/promoter in a possible targeted gene therapy scheme for prostate cancer. Meth- ods: PSA enhancer (PSAE) and promoter (PSAP) sequences were amplified using PCR method. CMV and T7 promoters were deleted from pcDNA3.1-Smac and replaced by the two specific fragments to generate pPSAE-PSAP-Smac. After transfection into different cell lines, the status of cells was observed. And then, we determined the relative concentration of Smac mRNA in RT-PCR. Results: The recombinant plasmid of pPSAE-PSAP-Smac was successfully constructed. And only the prostate cancer cell line PC-3 was suppressed after transfection with pPSAE-PSAP-Smac. However, other nonprostate lines were not. Moreover, the concentration of Smac mRNA regulated by PSA promoter and enhancer was higher in comparison to the CMV promoter-driven control vectors. Conclusion: An expression vector containing the Smac gene (based on elements of the PSA gene regulatory se- quences) has been developed and shown to function in prostate cancer cell lines which provides a solid platform for launching clin- ical studies. | Yu Wu Fuqing Zeng Liang Wang Yanbo Wang Guiyi Liao | 2007 | Journal of Nanjing Medical University2007，21，3： | 1 |
| 16 | Pose Determination and Plane Measurement Using a Trapezium显示文摘 | Duan Fuqing Wu Fuchao Hu Zhanyi | 2008 | Pattern Recognition Letters2008，29，3： | 1 |
| 17 | Clock component OsPRR59 delays heading date by repressing transcription of Ehd3 in rice显示文摘Heading date(or flowering time),an important agronomic trait in crop species,is closely associated with regional adaptation and yield.Members of the Pseudo-Response Regulator(PRR)family play key roles in regulating flowering.However,their role and molecular mechanism controlling heading date in rice is not very clear.Here,we identified rice OsPRR protein,OsPRR59,which delayed heading under longday conditions.OsPRR59 positively regulates yield by affecting plant height,secondary branches number per panicle,grain number per panicle,seed setting rate,and grain weight per plant.OsPRR59 is expressed in most tissues and its protein is localized to the nucleus.We also found that OsPRR59 directly binds to the promoter of Ehd3 to inhibit its expression.Compared with the WT,osprr59 ehd3 showed a significantly delayed heading phenotype,as did the ehd3 mutant.This was opposite to the phenotype of the osprr59 mutant,confirming that Ehd3 acted downstream of OsPRR59 in regulating rice flowering.Our results identified a direct regulator of Ehd3,and revealed a novel molecular mechanism of clock component OsPRR proteins in regulating heading date and provide a new genetic resource for fine-tuning heading date in rice. | Yupeng Wang Fuqing Wu Shirong Zhou Weiwei Chen Chenyan Li Erchao Duan Jiachang Wang Zhijun Cheng Xin Zhang Qibing Lin Yulong Ren Cailin Lei Xiuping Guo Ziming Wu Shanshan Zhu Jianmin Wan | 2022 | The Crop Journal2022，10，6： | 0 |
| 18 | The L-shell ionization of Ho and Os induced by electron impact显示文摘The electron-induced L-shell X-ray par- tial production cross sections, total production cross sections and mean ionization cross sections for Ho and Os have been measured as functions of electron energies from near threshold up to 36 keV by using a thin target with thick substrate technique. The influ- ence of the electrons reflected from the substrate was corrected by using the electron transport biparti- tion model. Also, the corrected measured results were compared with theoretical predictions proposed by Gryzinski and McGuire. | WU Zhangwen, GOU Chengjun, YANG Dailun, PENG Xiufeng, HE Fuqing & LUO Zhengming Key Laboratory for Radiation Physics and Technology of the Education Ministry of China Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China | 2006 | Chinese Science Bulletin2006，51，16： | 0 |
| 19 | 3-Hydroxybutyrate ameliorates insulin resistance by inhibiting PPARγSer273 phosphorylation in type 2 diabetic mice显示文摘3-Hydroxybutyrate(3HB)is a small ketone body molecule produced endogenously by the body in the liver.Previous studies have shown that 3HB can reduce blood glucose level in type 2 diabetic(T2D)patients.However,there is no systematic study and clear mechanism to evaluate and explain the hypoglycemic effect of 3HB.Here we demonstrate that 3HB reduces fasting blood glucose level,improves glucose tolerance,and ameliorates insulin resistance in type 2 diabetic mice through hydroxycarboxylic acid receptor 2(HCAR2).Mechanistically,3HB increases intracellular calcium ion(Ca^(2+))levels by activating HCAR2,thereby stimulating adenylate cyclase(AC)to increase cyclic adenosine monophosphate(cAMP)concentration,and then activating protein kinase A(PKA).Activated PKA inhibits Raf1 proto-oncogene serine/threonine-protein kinase(Raf1)activity,resulting in a decrease in extracellular signal-regulated kinases 1/2(ERK1/2)activity and ultimately inhibiting peroxisome proliferator-activated receptorγ(PPARγ)Ser273 phosphorylation in adipocytes.Inhibition of PPARγSer273 phosphorylation by 3HB altered the expression of PPARγregulated genes and reduced insulin resistance.Collectively,3HB ameliorates insulin resistance in type 2 diabetic mice through a pathway of HCAR2/Ca^(2+)/cAMP/PKA/Raf1/ERK1/2/PPARγ. | Yudian Zhang Zihua Li Xinyi Liu Xinyu Chen Shujie Zhang Yuemeng Chen Jiangnan Chen Jin Chen Fuqing Wu Guo-Qiang Chen | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 0 |
| 20 | Human-object interaction detection via interactive visual-semantic graph learning显示文摘Dear editor,Human-object interaction(HOI)detection is an important human-centric visual understanding task with several applications in visual monitoring,intelligent robot,etc.It aims at localizing and inferring interaction relationships between humans and objects in images.As a result of their success in object detection,many object detectors can be used to localize human and object instances.Therefore,the key to HOI detection mainly lies in the second part,namely interaction recognition. | Tongtong WU Fuqing DUAN Liang CHANG Ke LU | 2022 | Science China(Information Sciences)2022，65，6： | 0 |
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