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您的检索式：作者名="GENG XueFei"
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| 1 | An estimation model of time-varying origin-destination flows in expressway corridors based on unscented Kalman filter显示文摘On the basis of measurable time series of mainline and ramp flows from traffic counts and the assumption of travel time distributions, this research presents a dynamic system model and its on-line estimation algorithm for recursive estimation of time-varying origin-destination (OD) matrices in expressway corridors. The proposed model employs a macro-traffic flow model to estimate travel times of OD flows and uses parameters of the traffic model as state variables, which are added to the constrained function of the system. To improve the model efficiency, we revise the travel time distribution based on the feature of normal distribution. The research employs a newly developed filtering technique, called unscented Kalman filter. The proposed model is evaluated with simulation experiments. Numerical analyses with respect to the sensitivity of the selection of initial parameters on the estimation results indicate that the proposed model is sufficiently reasonable and stable for real-world appli-cations. | LI JunWei LIN BoLiang SUN ZhiHui GENG XueFei | 2009 | Science China(Technological Sciences)2009，52，7： | 0 |
| 2 | Preparation and evaluation of an enzymatic microreactor based on HILIC matrix for digestion and identification of proteins显示文摘In the present study,a new enzymatic microreactor is developed by immobilizing trypsin on the matrix-HILIC (hydrophilic interaction chromatography).The influences of different types of buffer solutions (borate,Tris·HCl,and phosphate),their concentrations and pH values on the immobilizing rate and the activity of immobilized trypsin were investigated.The optimal condition for off-line immobilization of trypsin on matix-HILIC was Tris·HCl buffer (20 mmol/L,pH 8.0),and then,under the optimal condition,an enzymatic microreactor was prepared by trypsin dynamically immobilized on HILIC matrix which was packed in a Peeksil column.It can be found that the microreactor possessed high hydrolytic activity according to the hydrolysis results of N-α-benzoyl-DL-arginine P-nitroanilide (BAPNA),a substrate of trypsin.Moreover,bovine serum albumin (BSA),cytochrome C and myoglobin as standard proteins were chosen to characterize the enzymolysis of the microreactor demonstrating the high enzymolysis efficiency by this enzymatic microreactor.Besides,the deactivated trypsin can be easily eluted from the matrix-HILIC to re-immobilize trysin,which is highly cost-effective in the experiment.All the results show that the new enzymatic microreactor associated with HPLC-MS techniques can realize online enzymolysis of proteins,and the time to analyze and identify the proteins is greatly shortened. | WANG ShanShan LI Qin LU XueFei LIU Tong ZHANG WeiBo GENG LiNa DENG YuLin | 2010 | Chinese Science Bulletin2010，55，10： | 0 |
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