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| 1 | Adiponectin downregulation is associated with volume overload-induced myocyte dysfunction in rats显示文摘目的: Adiponectin 被报导了在在体积的病理学的室的改变,而是 adiponectin 的角色期间施加保护的效果导致超载的心失败遗体不清楚。在这研究,我们在心脏的 myocyte 上调查了 adiponectin 的效果在 rats.Methods 的可收缩的机能障碍追随者体积超载: 体积超载被 infrarenal 主动脉静脉 cava 通过手术在老鼠导致管。老鼠是在 2- 的静脉内地管理的 adenoviral adiponectin, 6 星期、 9 星期的后面的管。adiponectin, adiponectin 受体(AdipoR1/R2 和 T-cadherin ) 和 AMPK 活动的蛋白质表示用西方的污点分析被测量。孤立的室的 myocytes 在管以后的 12 个星期被准备检验 myocytes 和细胞内部的 Ca 2+ transient.Results 的可收缩的表演: A-V 管在浆液和心肌的 adiponectin 层次导致了重要减小,心肌的 adiponectin 受体(AdipoR1/R2 和 T-cadherin ) 层次,以及心肌的 AMPK 活动。与这些变化一致,孤立的 myocytes 在房间弄短和细胞内部的 Ca 2+ 展出了重要消沉短暂。adenoviral adiponectin 的管理显著地增加了浆液 adiponectin 层次并且阻止了 myocyte 在管老鼠的可收缩的机能障碍。而且,有 recombinant adiponectin 的孤立的 myocytes 的预告的处理(2.5 μ g/mL ) 显著地在管老鼠改进了他们的可收缩的性能,但是没在控制或 adenoviral 有效果管理 adiponectin 的 rats.Conclusion : 这些结果表明在 adiponectin downregulation 和体积之间的积极关联导致超载的室的改变。Adiponectin 在体积起一个保护的作用导致超载的心失败。 | Li-li WANG Dori MILLER Desiree WANDERS Gayani NANAYAKKARA Rajesh AMIN Robert JUDD Edward E MORRI-SON Ju-ming ZHONG | 2016 | Acta Pharmacologica Sinica2016，37，2： | 4 |
| 2 | Methanol extract of Codium fragile inhibits tumor necrosis factor-ɑ-induced matrix metalloproteinase-9 and invasiveness of MDA-MB-231 cells by suppressing nuclear factor-κB activation显示文摘Objective:To evaluate whether the methanol extract of Codium fragile(MECF) regulates tumor necrosis factor-α(TNF-α)-induced invasion of human breast cancer MDA-MB-231 cells by suppressing matrix metalloproteinase-9(MMP-9).Methods:Reverse transcriptionpolymerase chain reaction(RT-PCR) and western blot analysis were performed to analyze the expression of MMP-9 and nuclear factor-κB(NF-κB) subunits,p65 and p50,and IκB in MDA-MB-231 cells.3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assay was used for cell viability.MMP-9 activity and invasion were measured by gelatin zymography and a matrigel invasion assay,respectively.NF- κB activity was measured by an electrophoretic mobility shift assay and luciferase activity.Results:MECF had no effects on cell viability up to a concentration of 100 μg/mL in human breast cancer MDA-MB-231 cells regardless of the presence of TNF-α.MDA-MB-231 cells that were stimulated with TNF-α showed a marked increase of invasion compared to the untreated control,whereas pretreatment with MECF downregulated the TNF-α-induced invasion of MDA-MB-231 cells.Additionally,zymography,western blot analysis,and reverse transcriptase-polymerase chain reaction(RT-PCR) confirmed that MECF decreased TNF-α-induced MMP-9 expression and activity which is a key regulator for cancer invasion.According to an electrophoretic morbidity shift assay,pretreatment with MECF in MDA-MB-231 cells significantly decreased the TNF-α-induced DNA-binding activity of nuclear factor- κB(NF- κB),which is an important transcription factor for regulating cancer invasion-related genes such as MMP-9.Furthermore,treatment with MECF sustained the expression of p65 and p50 in response to TNF-α in the cytosolic compartment.The luciferase assay demonstrated that MECF attenuated TNF-α-induced NF- κB luciferase activity.Conclusion:MECF exhibited its antiinvasive capability by downregulating TNF-α-induced MMP-9 expression,resulting from the suppression of NF- κB activity in the human breast cancer cell line MDA-MB-231. | Matharage Gayani Dilshara Rajapaksha Gedara Prasad Tharanga Jayasooriya Chang-Hee Kang Yung-Hyun Choi Gi-Young Kim | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 1 |
| 3 | Oxidative stress and essential fatty acids in patients with risk and established ARDS 显示文摘 | Kumar KV Rao SM Gayani R | 2000 | Clin Chim Acta2000，298，12： | 1 |
| 4 | The Criteria of Effective Teaching in a Changing Higher Education Context 显示文摘 | Marcia Delven Gayani Samarwickrema | 2010 | Higher Education Res Development2010，，2： | 1 |
| 5 | Imaging of the dif- fusion of single band 3 molecules on normal and nmtant e- rythrocytes 显示文摘 | Gayani C Kodippili Spector J | 2009 | Blood2009，，113： | 1 |
| 6 | Tadpoles as dengue mosquito ( Aedes aegypti ) egg predators显示文摘 | Gayan Bowatte Piyumali Perera Gayani Senevirathne Suyama Meegaskumbura Madhava Meegaskumbura | 2013 | Biological Control2013，，3： | 1 |
| 7 | Oxidant stress and essential fatty acids in patients with risk and established ARDS显示文摘 | Kumar KV Rao SM Gayani R | 2000 | Clin Chim Acta2000，298，12： | 1 |
| 8 | FTIR analysis of thermally processed PU foam显示文摘 | David W Hatchetta Gayani Kodippili John M Kinyanjui | 2005 | Polymer Degradation and Stability2005，87，： | 1 |
| 9 | Increased secretion of tissue inhibitors of metalloproteinases 1 and 2 (TIMPs ‐1 and ‐2) in fibroblasts are early indictors of oral sub‐mucous fibrosis and ageing显示文摘 | Gayani N. Pitiyage Kue P. Lim Emilios Gemenitzidis Muy‐Teck Teh Ahmad Waseem Stephen S. Prime Wanninayake M. Tilakaratne Farida Fortune E. K. Parkinson | 2012 | Journal of Oral Pathology & Medicine2012，，6： | 1 |
| 10 | Oxidant stress and essential fatty acids in patients with risk and established ARDS 显示文摘 | KUMAR KV RAO SM GAYANI R | 2000 | Clin Chim Acta2000，298，12： | 1 |
| 11 | Non-linear variable selection for artificial neural networks using partial mutual information显示文摘 | Robert J. May Holger R. Maier Graeme C. Dandy T.M.K. Gayani Fernando | 2008 | Environmental Modelling and Software2008，，10： | 1 |
| 12 | Cytomorphological Variations of Leishman-Donovan Bodies Found in Cutaneous Leishmaniasis Patients in Sri Lanka显示文摘Leishmania parasites undergo a dimorphic life cycle.Diagnosis of cutaneous leishmaniasis(CL)is primarily made by the microscopic identification of the amastigote form of the parasite in a smear.Therefore,correct identification of Leishman-Donovan(LD)bodies in smears is crucial for CL confirmation.The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani,which causes CL in Sri Lanka.A total of 125 smears,prepared from patients who met the positive diagnostic criteria of CL,were Giemsa stained and examined microscopically.The density,cellular location and all possible cytomorphological forms of the parasite were documented.A total of 10 different cytomorphological forms of LD bodies were identified.Among them,the round form was observed only intracellularly.The other morphological forms,namely the pear shape,cigarette shape,candle flame form,embryo shape,flagellate form,binary form and rosette form,were found only extracellularly.The spindle form and lens shape were observed in both intracellular and extracellular locations.L.donovani amastigotes have a wide range of morphologies besides their classical forms.Although Leishmania are considered as obligatory intracellular parasites,theymanage to survive successfully within the extracellular hostile conditions as well.Therefore,having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL. | Arumugam Murugananthan Kanchana P.Amarasinghe Gayanie W.Rathnaweera Ponnathurai T.Amalraajan Rajadurai Arulenthiran | 2023 | Infectious Microbes & Diseases2023，5，2： | 0 |
| 13 | Attendance to Non-communicable Disease Screening Services and Its Associated Factors among Rural Adults in Dimbulagala,Sri Lanka显示文摘Background:Non-Communicable Disease(NCD)incidence continues to increase globally causing high morbidity and mortality.Despite being a major health problem in Sri Lanka,low attendance is reported in free government NCD screening services(i.e.,Healthy life style center(HLC)and the Well Women Clinic(WWC)).This community based study aims to assess the attendance in NCD screening services,and its associated factors among 35 to 65 year adults in Dimbulagala Medical Officer of Health(MOH)area in Sri Lanka.Methods:The community based cross sectional study interviewed resident adults of the Dimbulagala MOH area in the North Central Province of Sri Lanka during August 2016.The sample of residents(n=575)were selected from 30 housing clusters representing the study setting and an interviewer administered questionnaire was used among residents of both genders to ascertain the data on attendance and factors associated with attendance to NCD screening clinics.Results:Five hundred and thirty-seven(n=537)questionnaires were successfully administered with a response rate of 93%.The mean age of the study population was 46.5(Standard Deviation(SD)=8.75)years.Majority(83.2%)of the study population had scored more than the middle value(i.e.,20)of the knowledge score and only 37.8% individuals have ever attended an NCD screening clinic.The“gender”and“awareness on HLC/WWC”are the most important factors determining the attendance to a screening clinic.Conclusions and recommendations:There is a gap between the respondents’NCD screening knowledge and attendance to NCD screening services.Main factors associated with attending NCD screening clinics were gender of the study participants and their awareness of HLC or WWC. | Dehiwattage Eroma Gayani Fernando Sumal Nandasena | 2019 | Journal of Health Science2019，7，1： | 0 |
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