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| 1 | Microfluidic actuators based on temperature-responsive hydrogels显示文摘The concept of using stimuli-responsive hydrogels to actuate fluids in microfluidic devices is particularly attractive,but limitations,in terms of spatial resolution,speed,reliability and integration,have hindered its development during the past two decades.By patterning and grafting poly(N-isopropylacrylamide)PNIPAM hydrogel films on plane substrates with a 2μm horizontal resolution and closing the system afterward,we have succeeded in unblocking bottlenecks that thermo-sensitive hydrogel technology has been challenged with until now.In this paper,we demonstrate,for the first time with this technology,devices with up to 7800 actuated micro-cages that sequester and release solutes,along with valves actuated individually with closing and opening switching times of 0.6±0.1 and 0.25±0.15 s,respectively.Two applications of this technology are illustrated in the domain of single cell handling and the nuclear acid amplification test(NAAT)for the Human Synaptojanin 1 gene,which is suspected to be involved in several neurodegenerative diseases such as Parkinson’s disease.The performance of the temperature-responsive hydrogels we demonstrate here suggests that in association with their moderate costs,hydrogels may represent an alternative to the actuation or handling techniques currently used in microfluidics,that are,pressure actuated polydimethylsiloxane(PDMS)valves and droplets. | Loïc D'Eramo Benjamin Chollet Marie Leman Ekkachai Martwong Mengxing Li Hubert Geisler Jules Dupire Margaux Kerdraon Clémence Vergne Fabrice Monti Yvette Tran Patrick Tabeling | 2018 | Microsystems & Nanoengineering2018，4，1： | 3 |
| 2 | Identification of genes encoding N-glycan processing beta-Nacetylglucosaminidases in Trichoplusia ni and Bombyx mori: Implications for glycoengineering of baculovirus expression systems显示文摘 | GEISLER C JARVIS D L | 2010 | Biotechnol Prog2010，26，1： | 1 |
| 3 | Laparoscopic exp loration for the clinically undetected hernia in infancy and childhood显示文摘 | Geisler D P Jegathesan S Parmley M C | 2001 | Am J Surg2001，182，6： | 1 |
| 4 | A cochlear model using feed-forward outer-hair-cell forces 显示文摘 | Geisler C D Sang C | 1995 | Hearing Research1995，86，13： | 1 |
| 5 | Olfactory event-related potentials and aging: normative data显示文摘 | MURPHY C MORGAN C D GEISLER M W | 2000 | Int J Psychophysiol2000，36，： | 1 |
| 6 | Olfactory event-related potentials: older males demonstrate the greatest deficits显示文摘 | MORGAN C D COVINGTON J W GEISLER M W | 1997 | Electroencephalogr Clin Neurophysiol1997，104，： | 1 |
| 7 | Structureof poliovirus type 2Lansing complexed with antiviral a-gent SCH48973:comparison of the structural and bio-logical properties of three poliovirus serotypes显示文摘 | Lentz K N Smith A D Geisler S C | 1997 | Structure1997，5，7： | 1 |
| 8 | Interneuron diversity aeries:Circuit complexity and axon wiring economy of cortical interneurons显示文摘 | Buzsáki G Geisler C Henze D A | 2004 | Trends Neurosci2004，27，4： | 1 |
| 9 | The influence of root pruning and water relation, net photosynthesis, and growth of young ' Golden Delicious' apple trees显示文摘 | Geisler D Ferrec D C | 1984 | Journal of the American Society for Horticultural Science1984，109，： | 1 |
| 10 | Bubble dynamics, shock waves and sonoluminescence 显示文摘 | OHL C D KURZ T GEISLER R | 1999 | Philosophical Transactions of The Royal Society1999，357，1751： | 1 |
| 11 | Olfactory event related potentials and aging: normative data显示文摘 | MURPHY C MORGAN C D GEISLER M W | 2000 | Int J Psychophysiol2000，36，： | 1 |
| 12 | Arthroscopic microscopy of articular cartilage using optical coherence tomography 显示文摘 | Chu C R Lin D Geisler J L | 2004 | Am J Sports Med2004，32，3： | 1 |
| 13 | Hippocampal place cell assemblies are speed-controlled oscillators显示文摘 | Geisler C Robbe D Zugaro M | 2007 | Proc of the National Academy of Sciences2007，104，19： | 1 |
| 14 | Identification of genes encoding N-glycan processing β-N- acetylglueosaminidases in Trichoplusia ni and Bombyx mori: Implications for glycoengineering of baculovirus expression systems 显示文摘 | GEISLER C JARVIS D L | 2010 | Biotechnology Progress2010，26，1： | 1 |
| 15 | Some neural mechanisms in the inferior colliculus of the cat which may be relevant to localization of a sound source显示文摘 | ROSE J E GROSS N B GEISLER C D | 1966 | J Neurophysiol1966，29，2： | 1 |
| 16 | The determination of sulphate in fluid inclusions using the MOLE Raman microprobe- Application to a Keuper halite and geochemical consequences 显示文摘 | Dubessy J Geisler D Kosztolany C | 1983 | Geochimica et Cosmochimica Acta1983，47，： | 1 |
| 17 | The influence of root pruning on water relations,net photosynthesis,and growth of young Golden Delicious apple trees显示文摘 | Geisler D Ferree D C | | 0，，06： | 1 |
| 18 | Outcome of Reproductive Age Women with Stage IA or IC Invasive Epithelial Ovarian Cancer Treated with Fertility-Sparing Therapy显示文摘 | Jeanne M Schilder Amy M Thompson Paul D DePriest Frederick R Ueland Michael L Cibull Richard J Kryscio Susan C Modesitt Karen H Lu John P Geisler Robert V Higgins Paul M Magtibay David E Cohn Matthew A Powell Christina Chu Frederick B Stehman John van Nag | 2002 | Gynecologic Oncology2002，，： | 1 |
| 19 | A fused lobes gene encodes the processing beta N acetylglucosaminidase in Sf9 cells显示文摘 | Geisler C Aumiller J J Jarvis D L | 2008 | J Biol Chem2008，283，11： | 1 |
| 20 | Olfactory event-related potentials and aging:normative data显示文摘 | MURPHY C MORGAN C D GEISLER M W etal | 2000 | Int J Psychophysiol2000，36，： | 1 |
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