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| 1 | Polygonatum sibiricum polysaccharides protect against obesity and non-alcoholic fatty liver disease in rats fed a high-fat diet显示文摘Polygonatum sibiricum is a traditional medicinal and dietary plant of the family Liliaceae. The main functional macromolecules of P. sibiricum are polysaccharides, which function in antioxidation and regulating immunity. Previous studies have shown that insulin resistance(IR), oxidative stress, and inflammation are important factors in the induction of lipid metabolic diseases such as obesity. Therefore, in this study, we established a high-fat diet-induced rat model of obesity and nonalcoholic fatty liver disease(NAFLD) to explore the potential protective effect of P. sibiricum polysaccharides(PSPs) and the mechanisms behind it. After 4 weeks of high-fat diet feeding to induce obesity, the rats were treated with different doses of PSP solution or distilled water for 6 weeks. Compared with untreated obese rats, PSP-treated obese rats showed a decrease in body weight, serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels, hepatic aspartate aminotransferase and alanine aminotransferase activity, hepatic malondialdehyde content, and hepatic levels of the pro-inflammatory factors tumor necrosis factor-α, interleukin-1β, and interleukin-6, as well as increased serum high-density lipoprotein cholesterol levels and hepatic superoxide dismutase, catalase, and glutathione peroxidase activity. Pathological analysis and immunoblotting of the liver tissues indicated that mechanistically, PSPs reduced obesity and NAFLD in rats by upregulating insulin receptor expression, increasing adenosine monophosphate-activated protein kinase phosphorylation, and downregulating sterol regulatory element-binding protein 2 and low-density lipoprotein receptor expression, thus promoting lipid metabolism, decreasing body weight, and reducing inflammation and oxidative stress caused by lipid accumulation. Based on these results, PSPs may have the potential to reduce obesity and NAFLD associated with a high-fat diet. | Li Zeng Fangwei Zhong Zhiliang Chen Gengxi Li Qi Zhu | 2022 | Food Science and Human Wellness2022，11，4： | 3 |
| 2 | C/EBPαp30 plays transcriptional regulatory roles distinct from C/EBPαp42显示文摘CCAAT/enhancer 有约束力的蛋白质α(C/EBP α) 是 transcriptional 禁止细胞增殖的规章的因素,和其他的翻译开始生产二多肽, C/EBP α p 30 并且 C/EBP α p 42。由表示介绍, C/EBP α p 30specifically 禁止了基因的一个唯一的集合,这被揭示,包括 MPP11, p84N5 和 SMYD2,它没被 C/EBP α p 影响 42 在两根 QSG-7701 hepatocyte 房间线和 QGY-7703 hepatoma, cells.Semi 量的 RT-PCR 分析独立地证实了这些结果。Chromatinimmunoprecipitation 试金显示出 30 比 C/EBP α p 更强烈绑了在这些基因的倡导者的那 C/EBP α p 42。在 C/EBP α是调整的 down 的临床的 hepatoma 在样品,所有三基因明确地由 30 在上面的 C/EBP α p 禁止了调整。然而, MPP11, p84N5 和 SMYD2genes 的压抑可能直接不涉及 C/EBP α p 30-mediated 生长抑制。我们的数据建议 30 调整的那 C/EBP α p 目标基因的一个唯一的集合并且多于 C/EBP α p 的 dominant-negativeregulator 42。 | Chunxi Wang~1 Xiaotao Chen~1 Yanping Wang~1 Jialei Gong~1 Gengxi Hu ~1State Key Laboratory of Molecular Biology institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China | 2007 | Cell Research2007，17，4： | 2 |
| 3 | Binding of IFITM1 enhances the inhibiting effect of caveolin-1 on ERK activation显示文摘(IFITM1 ) 导致干扰素的 transmembrane 蛋白质 1 是干扰素的一个必要调停人 -- 导致的反增长。这里,我们报导了在之间的相互作用 IFITM1 和 caveolin-1,和他们细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) 上的禁止的规章的功能(CAV-1 ) 。染色结果的 immunofluorescence 显示出在血浆膜的 caveolae 局部性的那 IFITM1 并且能与 CAV-1 交往。删除 mutagenesis 清楚地表明恐水病的 transmembrane 领域为在 IFITM1 和 CAV-1 之间的相互作用负责。CAV-1 在英皇家空军之阶级最低之兵,和随后调停英皇家空军之阶级最低之兵的抄写的 phosphorylation 上有禁止的效果,这被报导了。我们的学习在英皇家空军之阶级最低之兵激活上显示出 IFITM1-1-enhanced 和 CAV-1-enhanced CAV-1 的禁止的效果的相互作用,而 IFITM1 没直接激活英皇家空军之阶级最低之兵。这禁止的效果被用 RNA 干扰将内长的 CAV-1 击倒进一步证实。这些结果表明在 IFITM1 和 CAV-1 之间的相互作用能在英皇家空军之阶级最低之兵激活上提高 CAV-1 的禁止的效果。 | Ye Xu Guohua Yang Gengxi Hu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，6： | 2 |
| 4 | The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid显示文摘 | Chaoyin Cui Jinxiu Shi Lijian Hui Huifeng Xi Zhuoma Quni Tsedan and Gengxi Hu | 2002 | J Gen Virol2002，83，： | 1 |
| 5 | 3D Printing of Nacre-Inspired Structures with Exceptional Mechanical and Flame-Retardant Properties显示文摘Flame-retardant and thermal management structures have attracted great attention duc to the requirement of high-temperature exposure in industrial,aerospace,and thermal power fields,but the development of protoctive fire-retardant structures with complex shapes to fit arbitrary surfaces is still challenging.Herein,we reported a rotation-blade casting-assisted 3D printing process to fabricate nacre-inspired structures with exceptional mechanical and flame-retardant properties,and the relatedi fundamental mechanisms are studied.3-(Trimethoxysilyl)propyl methacrylate(TMSPMA)modified boron nitride nanoplatelets(BNs)were aligned by rotation-blade casting during the 3D printing process to build the'hrick and mortar'architecture.The 3D printed structures are more lightweight,while having higher fracture toughness than the natural nacre,which is attributed to the crack deflection,aligned BN(a-BNs)bridging,and pull-outs reinforced structures by the covalent bonding between TMSPMA grafted a-BNs and polymer matrix.Thermal conductivity is enhanced by 25.5 times compared with pure polymer and 5.8 times of anisotropy due to the interconnection of a-BNs.3D printed heat-exchange structures with vertically aligned BNs in complex shapes were demonstrated for efficient thermal control of high-power light-emitting diocles.3D printed helmet and armor with a-BNs show exceptional mechanical and fire-retardant properties,demonstrating integrated mechanical and thermal protection. | Yang Yang Ziyu Wang Qingqing He Xiangjia Li Gengxi Lu Laiming Jjiang Yushun Zeng Brandon Bethers Jie Jin Shuang Lin Siqi Xiao Yizhen Zhu Xianke Wu Wenwu Xu Qiming Wang Yong Chen | 2022 | Research2022，，2： | 1 |
| 6 | Central neurocytoma: a clinicopathological and neuroradiological study显示文摘 | Bo Zhang Boning Luo Zhongwei Zhang Gengxi Sun Jianming Wen | 2004 | Neuroradiology2004，，11： | 1 |
| 7 | Central neurocytoma: a clinicopathological and neuroradiological study显示文摘 | Bo Zhang Boning Luo Zhongwei Zhang Gengxi Sun Jianming Wen | 2004 | Neuroradiology2004，，11： | 1 |
| 8 | PEG10 directly regulated by E2Fs might have a role in the development of hepatocellular carcinoma显示文摘 | Chaochen Wang Ying Xiao Zhonghua Hu Yibin Chen Na Liu Gengxi Hu | 2008 | FEBS Letters2008，，18： | 1 |
| 9 | Fine analysis of the chromatin structure of yeast RNA poly-meraseⅡtranscription terminators显示文摘In order to study the functional structure of thetranscription terminators and the mechanism oftermination,a survey of the chromatin structure,including the location of DNase I hypersensitive sitesand the nucleosome arrangement,of yeast ADH1 andFLP terminators was made.The results show that thereis no relationship between the function of theterminators and the existence of DNase I hypersensitivesites.However,it is found that there is always anucleosome at the immediate upstream of the transcrip-tional termination sites.As a control,the chromatinstructures of the pBR322 DNA fragments on the yeastshutter vectors are also investigated at the same time.The random nucleosome arrangement on the bacterialDNA in yeast agrees with the published reports.A newhypothesis,about the mechanism of transcriptionaltermination is put forward and the reason of differentnucleosome arrangement on the DNAs which areori-ginally from different species in yeast is discussed. | HU GENGXI, YTJNHUA YU AND DARKEN KUANG Shanghai Institute of Cell Biology, Academia Sinica. 320 Yue-Yang Road, Shanghai 200031, China | 1991 | Cell Research1991，2，1： | 0 |
| 10 | ecent Advances in the Science of Burst Wave Lithotripsy and Ultrasonic Propulsion显示文摘Objective.Retinal degeneration involving progressive deterioration and loss of function of photoreceptors is a major cause of permanent vision loss worldwide.Strategies to treat these incurable conditions incorporate retinal prostheses via electrically stimulating surviving retinal neurons with implanted devices in the eye,optogenetic therapy,and sonogenetic therapy.Existing challenges of these strategies include invasive manner,complex implantation surgeries,and risky gene therapy.Methods and Results.Here,we show that direct ultrasound stimulation on the retina can evoke neuron activities from the visual centers including the superior colliculus and the primary visual cortex(V1),in either normal-sighted or retinal degenerated blind rats in vivo.The neuron activities induced by the customized spherically focused 3.1 MHz ultrasound transducer have shown both good spatial resolution of 250μm and temporal resolution of 5 Hz in the rat visual centers.An additional customized 4.4 MHz helical transducer was further implemented to generate a static stimulation pattern of letter forms.Conclusion.Our findings demonstrate that ultrasound stimulation of the retina in vivo is a safe and effective approach with high spatiotemporal resolution,indicating a promising future of ultrasound stimulation as a novel and noninvasive visual prosthesis for translational applications in blind patients. | Xuejun Qian Gengxi Lu Biju B.Thomas Runze Li Xiaoyang Chen K.Kirk Shung Mark Humayun Qifa Zhou | 2022 | Biomedical Engineering Frontiers2022，3，1： | 0 |
| 11 | microR-1294-5p inhibits glycolytic metabolism of non-small cell lung cancer cells via targeting TMPRSS11B显示文摘Non-smallcell lung cancer(NSCLC)cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death.MicroRNAs(miRNAs)have been known to play pivotal roles in NSCLC development partly through mediating glycolysis.However,only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC.TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues.The function of miR-1294-5p was determined in NSCLC cells by cell proliferation,glucose uptake,lactate release,and Extracellular Acidification Rate(ECAR)assays.The target of miR-1294-5p was predicted by TargetScan and miRDB,which was further validated by flow cytometry analysis,RT-qPCR,western blotting,a dual-luciferase reporter assay,and RNA immunoprecipitation(RIP)assay.In the present study,it was found that miR-1294-5p was a significantly downregulated miRNA in lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC).The overexpression of miR-1294-5p inhibited glycolysis,lactate export,ECAR,and cell proliferation in NSCLC cells.Analysis with bioinformatic tools,Western Blotting,RT-qPCR,flow cytometry analysis,dual-luciferase reporter assay,and RIP assay showed that miR-1294-5p directly bound to complementary sites in the 3’-Untranslated Region(UTR)of TMPRSS11B resulted in downregulation of TMPRSS11B expression.In addition,transfection of recombinant TMPRSS11B rescued the functions of miR-1294-5p on glycolysis and proliferation of NSCLC cells.The findings provided novel insights for understanding the regulation of glycolytic metabolism in NSCLC. | JI ZHU XIYING BO GENGXI JIANG SHIHUA YAO TIEJUN ZHAO LING CHEN | 2021 | BIOCELL2021，45，3： | 0 |
| 12 | WHAT IS BASIC RESEARCH?显示文摘The main body of basic research in contemporary science has been evolved from mental adventures motivated by an individual’ s curiosity in Newtonian times into large-scale and normally wellorganized social behaviors driven by keen desires and always oriented by primary social demands. By now, very probably there are few people still claiming that basic research is unnecessary in this country. And the problem needing our discussion is how to develop our basic research projects most effectively by using the limited funding resources available. First of all, file current S&T system needs an institutional facelift and the rampant | Hu Gengxi(Shanghai Institute of Cell Biology, CAS) | 1998 | Bulletin of the Chinese Academy of Sciences1998，12，2： | 0 |
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