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| 1 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 2 | Nanostructured ZnO for biosensing applications显示文摘Based on the unique properties, nanostructured ZnO could provide a stable immobilization for biomolecules retaining their biological activity. It has been recently developed as a nice candidate for the construction of biosensors with enhanced analytical performance. In this paper, we reviewed the progress in adapting nanostructured ZnO for several predominantly in biosensing applications based on enzymic reaction, immunoreaction, and molecular compitation. We also described several important considerations when working with nanostructured ZnO mainly centered on the fabrications of ZnO and appropriate strategies for biosensor construction (e.g. modified electrodes and multilayered immobilization). | XU ChunXiang YANG Chi GU BaoXiang FANG ShengJiang | 2013 | Chinese Science Bulletin2013，58，21： | 4 |
| 3 | Effects of Mg Incorporation on Microstructure and Optical Properties of ZnO Thin Films Prepared by Sol-gel Method显示文摘Well-crystallized MgZnO alloy thin films with hexagonal wurtzite structure were fabricated by sol-gel method. With the band gap increases, the surface roughness and the grain size reduces. It is worth noting that the intensity of the band-edge luminescence of Mg doped films enhances with the increase of the Mg content. The microstructure and photoluminescence mechanism have been discussed based on X-ray diffraction patterns, atomic force microscopy images, ultraviolet-visible absorption spectra, photoluminescence spectra and Fourier transform infrared spectra. | Rui Ding Chunxiang Xu Baoxiang Gu Zengliang Shi Haitao Wang Long Ba Zhongdang Xiao | 2010 | Journal of Materials Science & Technology2010，26，7： | 1 |
| 4 | 显示文摘 | Xu Chunxiang Gu Baoxiang Yang Chi | 2011 | Southeast Univ (Med Sci Edi) :2011，30，1： | 1 |
| 5 | Pre-expanded Muscle-sparing Latissimus Dorsi Flaps for Reconstruction of Severe Scar Contractures on the Anterior Chest显示文摘Objective To investigate the utility of pre-expanded muscle-sparing latissimus dorsi flaps in the reconstruction of deformities secondary to severe scar contractures on the anterior chest.Methods The function of the latissimus dorsi was preserved with blood supply from the main or lateral branch of the thoracodorsal artery.The entire treatment period was divided into two stages,during which segmental latissimus dorsi flaps were pre-expanded in stage I and anterior chest scar deformities were reconstructed in stage II.During stage I,the musculocutaneous perforators arising from the lateral branch of the thoracodorsal artery were determined by ultrasound preoperatively;the flap design included the anterior segment of the latissimus dorsi supplied by the musculocutaneous perforators from the lateral branch;and a tissue expander was placed following flap dissection and then infused with saline intermittently for 4–6 months.In stage II,the chest scars were excised,and breast tissues were repositioned;the continuity of the medial branch of the thoracodorsal nerve to the muscle was preserved when reconstruction was performed using the segmental latissimus dorsi flaps supplied by the main or lateral branch of the thoracodorsal artery.Results From October 2010 to October 2019,21 patients(on 24 sides)underwent reconstructive procedures for extensive scar contractures on the anterior chest.All flaps survived,and their donor sites were sutured directly.During a follow-up of 3 months to 8 years,the flaps became soft and exhibited color similar to that of the adjacent tissues.The limited neck and shoulder movements improved,and postoperatively,all female patients were satisfied with the shape of their breasts.Additionally,neither apparent weakening on the adduction,internal rotation,or extension strength of the shoulder joint on the affected side nor marked depression deformity in the back was observed.Conclusion Pre-expanded muscle-sparing latissimus dorsi flaps with blood supply from the main or lateral branch of the thoracodorsal artery proved to be a desirable option for the reconstruction of extensive scar contractures on the anterior chest. | Zhichao WANG Dujuan LIU Shuchen GU Baoxiang TIAN Tao ZAN Bin GU | 2020 | Chinese Journal Of Plastic and Reconstructive Surgery2020，2，2： | 0 |
| 6 | Single-cell dissection,hdWGCNA and deep learning reveal the role of oxidatively stressed plasma cells in ulcerative colitis显示文摘Ulcerative colitis(UC)develops as a result of complex interactions between various cell types in the mucosal microenvironment.In this study,we aim to elucidate the pathogenesis of ulcerative colitis at the single-cell level and unveil its clinical significance.Using single-cell RNA sequencing and high-dimensional weighted gene co-expres-sion network analysis,we identify a subpopulation of plasma cells(PCs)with significantly increased infiltration in UC colonic mucosa,characterized by pronounced oxidative stress.Combining 10 machine learning approaches,we find that the PC oxidative stress genes accurately distinguish diseased mucosa from normal mucosa(independent external testing AUC=0.991,sensitivity=0.986,specificity=0.909).Using MCPcounter and non-negative matrix factorization,we identify the association between PC oxidative stress genes and immune cell infiltration as well as patient heterogeneity.Spatial transcriptome data is used to verify the infiltration of oxidatively stressed PCs in colitis.Finally,we develop a gene-immune convolutional neural network deep learning model to diagnose UC mucosa in different cohorts(independent external testing AUC=0.984,sensitivity=95.9%,specificity=100%).Our work sheds light on the key pathogenic cell subpopulations in UC and is essential for the development of future clinical disease diagnostic tools. | Shaocong Mo Xin Shen Baoxiang Huang Yulin Wang Lingxi Lin Qiuming Chen Meilin Weng Takehito Sugasawa Wenchao Gu Yoshito Tsushima Takahito Nakajima | 2023 | Acta Biochimica et Biophysica Sinica2023，55，11： | 0 |
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