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| 1 | Microelectronics-embedded channel bridging and signal regeneration of injured spinal cords显示文摘Due to the dificulty in spinal cord regeneration with biological methods,the microelectronic neural bridge,a new concept based on microelectronic technology,is presented.The microelectronic system has been realized in the forms of hybrid and integrated circuits.The integrated circuits forneural signal detection,stimulation,and regeneration are realized in a CMOS process.In animal experiments with 100 toads,48 rats,and 3 rabbits,nerve signals have been successfully detected from spinal cords and sciatic nerves,and functional electrical stimulation has been carried out for spinal cords and sciatic nerves.When the microelectronic system is bridged between the controlling and stimulated nerve,the relevant motion of legs and nerve signal waveforms,which are stimulated by the evoked or spontaneous nerve signal through such a system,have been observed.Therefore,the feasibility of the presented method was demonstrated. | Zhigong Wang Xiaosong Gu Xiaoying Lǔ Zhenglin Jiang Wenyuan Li Guangming Lǔ Yufeng Wang Xiaoyan Shen Xintai Zhao Huiling Wang Zhenyu Zhang Hongmei Shen Yang Wu Weixing Shen Jingyang Zhang Dong Chen Xiaoyi Mao Huaxiang Shen | 2009 | Progress in Natural Science:Materials International2009，19，10： | 5 |
| 2 | A 3D Geological Model Constrained by Gravity and Magnetic Inversion and its Exploration Implications for the World-class Zhuxi Tungsten Deposit, South China显示文摘The Zhuxi tungsten deposit in Jiangxi Province,South China,contains a total W reserve of about 2.86 Mt at an average grade of 0.54 wt%WO3,representing the largest W deposit in the world.Numerous studies on the metallogeny of the deposit have included its timing,the ore-controlling structures and sedimentary host rocks and their implications for mineral exploration.However,the deep nappe structural style of Taqian-Fuchun metallogenic belt that hosts the W deposit,and the spatial shape and scale of deeply concealed intrusions and their sedimentary host rocks are still poorly defined,which seriously restricts the discovery of new deposits at depth and in surrounding areas of the W deposit.Modern 3 D geological modeling is an important tool for the exploration of concealed orebodies,especially in brownfield environments.There are obvious density contrast and weak magnetic contrast in the ore-controlling strata and granite at the periphery of the deposit,which lays a physical foundation for solving the 3 D spatial problems of the ore-controlling geological body in the deep part of the study area through gravity and magnetic modeling.Gravity data(1:50000)and aeromagnetic data(1:50000)from the latest geophysical surveys of 2016-2018 have been used,firstly,to carry out a potential field separation to obtain residual anomalies for gravity and magnetic interactive inversion.Then,on the basis of the analysis of the relationship between physical properties and lithology,under the constraints of surface geology and borehole data,human-computer interactive gravity and magnetic inversion for 18 cross-sections were completed.Finally,the 3 D geological model of the Zhuxi tungsten deposit and its periphery have been established through these 18 sections,and the spatial shape of the intrusions and strata with a depth of 5 km underground were obtained,initially realizing―transparency‖for ore-controlling bodies.According the analysis of the geophysical,geochemical,and geological characteristics of the Zhuxi tungsten deposit,we discern three principles for prospecting and prediction in the research area,and propose five new exploration targets in its periphery. | YAN Jiayong LÜ Qingtian QI Guang FU Guangming ZHANG Kun LAN Xueyi GUO Xin WEI Jin LUO Fan WANG Hao WANG Xu | 2020 | Acta Geologica Sinica(English Edition)2020，94，6： | 3 |
| 3 | Development of porcine embryos and offspring after itttracytoplasmic sperm injection withliposome transfected or nontransfected sperm into in vitro matured oocytes显示文摘 | Lai L Qingyuan S Guangming W | 2001 | Zygote2001，9，： | 1 |
| 4 | Using the wavelet transform to detect singularities of the thin bed seismic signals 显示文摘 | Guangming Z Qingchun L | 2000 | SEG Expanded Abstracts2000，，： | 1 |
| 5 | Astrocyte reactivity in related brain regions in a mouse model of MPTP-induced Parkinson's disease显示文摘BACKGROUND:Severe injury to dopaminergic neuronal cell bodies and their axon terminals in the substantia nigra pars compacta(SNC) has been observed in both Parkinson's disease(PD) patients or in 1-methy-4-phenyl-1,2,3,6-tetrahydropyrindine(MPTP)-induced PD animal models, but only slight injury occurs in the adjacent ventral tegmental area(VTA).The mechanisms underlying this selective injury remain poorly understood. OBJECTIVE:To comparatively observe astrocyte reactivity in the SNC,caudate putamen(CPu), VTA,and frontal association cortex(FrA). DESIGN,TIME AND SETTING:A cellular and molecular biology,randomized,controlled experiment was performed at the Institute of Neurobiology,Department of Human Anatomy, Medical School of Nantong University,between December 2006 and September 2008. MATERIALS:A total of 80 healthy adult male C57BL/6 mice were included in this study.MPTP was purchased from Sigma,USA. METHODS:Mice were randomly divided into a model group(n=64) and a sham-operated group (n=16).PD was induced in the mice from the model group by intraperitoneal injection of 20 mg/kg MPTP,once every three hours,for a total of 4 times. MAIN OUTCOME MEASURES:Tyrosine hydroxylase(TH)-immunoreactive neurons and glial fibrillary acidic protein(GFAP)-immunoreactive astrocytes were examined by dual immunofluorescence labeling.GFAP-immunoreactive astrocytes in the CPu and FrA were determined by immunofluorescent staining.GFAP mRNA expression in the SNC,CPu,VTA,and FrA was detected using real-time polymerase chain reaction.TH protein levels in the TH-immunoreactive axon terminals of the CPu and FrA were detected by Western blotting. RESULTS:Numbers of TH-immunoreactive neurons in the SNC,and TH protein level in the CPu, markedly decreased(by approximately 68%) 1 day after MPTP injection,and gradually increased at 3 days.Simultaneously,astrocyte reactivity was strengthened,in particular at 7 days.However, after MPTP injection,decreases in the numbers of TH-immunoreactive neurons in the VTA,and TH protein levels in the FrA,were less apparent(approximately 15%).Also,no obvious astrocyte reactivity was observed. CONCLUSION:In a mouse model of PD,astrocyte reactivity was apparent in the SNC and CPu, but not the VTA or FrA.In addition,astrocyte reactivity was greater in regions where injury to dopaminergic neurons was more severe. | Zhijun Zhang Chunlin Xia Yulin Dong Guangming Lü Juan Liu Lin Ding Hengjian Ni | 2009 | Neural Regeneration Research2009，4，2： | 1 |
| 6 | Adaptive wavelet thresholding for time varying SNR signal denoising 显示文摘 | S Guangming L Xiaoping J Licheng Z Wei | 2002 | IEEE International Symposium on Circuits and Systems ISCAS 20022002，4，： | 1 |
| 7 | Overcoming muhidrug - re- sistance in vitro and in vivo using the novel P - glycoprotein inhibi- tor1416显示文摘 | Yan XU Feng ZHI Guangming X L | 2012 | Biosci2012，，32： | 1 |
| 8 | The comparative study of tumor angiogenesis and CT enhancement in pancreatic carcinoma显示文摘 | Zhongqiu W Guangming L Jieshou L | 2004 | Europ J Radio2004，49，3： | 1 |
| 9 | MorphoSys:an integrated reconfigurable system for data-parallel and computation-intensive applications 显示文摘 | Singh H Ming-Hau L Guangming L | 2000 | IEEE Transactions on Computers2000，49，5： | 1 |
| 10 | Three-dimensional reconstruction of human spinal cord based on histological serial sections显示文摘BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at ×25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with 'Curves', 'Autocontrast', ' Gray scale 8 bit', 'Invert', 'Image resize to 50%' steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and mac- rosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord. | Guangming Lü Huiqun Wu Lemin Tang Xiao Han Dafeng Ji Xiaosong Gu | 2008 | Neural Regeneration Research2008，3，7： | 1 |
| 11 | Long-term therapeutic effects of corticoamygdalohippocampectomy for bilateral mesial temporal lobe epilepsy 显示文摘 | Guangming Z Wenjing Z Jiuluan L | 2013 | Surg Neurol Int2013，4，： | 1 |
| 12 | Cystic schwannoma of the pancreas显示文摘 | Guangming Tan Kenneth Vitellas Carl Morrison Wendy L Frankel | 2003 | Annals of Diagnostic Pathology2003，，5： | 1 |
| 13 | Nonlocally Centralized Sparse Representation for Image Restoration 显示文摘 | Weisheng D Lei Z Guangming S Xin L | 2013 | IEEE Transac- tions on Image Processing2013，22，4： | 1 |
| 14 | Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats显示文摘BACKGROUND: There are many methods for myelin staining,mordant,or the special reaction of osmic acid with lipoid is used according to different principles.The commonly used methods are classic Well staining ,classic lithium carbonate-haematine staining,fast green staining,silver staining ,etc.Luxol Fast Blue can brightly stain myelin sheath,and has certain specificity .The background can be very clean if there is proper differentiation,whereas Luxol Fast Blue is cheap and convenient to operate,thus it is an ideal staining reagent for routine myelin sheath.OBJECTIVE: To show the coricospinal tract of normal adult rats with Luxol Fast Blue shaining method.DESIGN:A repetitive measurement design.SETTINGS: Institute of Nuerobiology,Nantong University;Department of Rehabilitation Medicine,Affiliated Hospital of Nantong University.MATERIALS: Six healthy adult male SD rats of clean dergree,weighing averagely 300 g.were provided by the experimental animal center of Nantong University.1 g/L Luxol Fast Blue solution was provided by Sigma Company;Leica CM1900 cryostat microtome by Leica Company;Leica DMR microscope by Leica Company.METHODS:The experiment was carried out in the Staff Room of Human Anatomy,Nantong University in May 2005.The rats were given intraperitoneal injection of combined anesthetic(2 mL/kg),then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed,then changed to phosphate buffer(pH 7.4)containing 300 g/L saccharu at 4 ℃.and stayed overnight,tissue blocks at pyramid,decussation of pyramid and cervical,thoracic,lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections(30 μm) after sedimentation,the sections were dried at room temperature.The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method,and observed under Leica DMR microscope.MAIN OUTCOME MEASURES:Positive fibers in Luxol Fast Blue staining.RESULTS:After the Luxol Fast Blue staining,the labeled myelinated nerve fibers were bright blue.They located in the pyramid,decussation of pyramid and the ventral part of posterior funiculus in cervical,thoracic,lumbar and sacral segments of spinal cord.CONCLUSION:Luxol Fast Blue staining method may manifest the distribution of corticospinal tract with clear distinct in adult rats. | Su Liu1, Guangyu Shen1, Guangming Lü2, Xiaosong Gu3 1Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China 2Department of Human Anatomy, Nantong University, Nantong 226001, Jiangsu Province, China 3Jiangsu Key Laboratory of Nerve Regeneration, Nantong 226001, Jiangsu Province, China | 2006 | Neural Regeneration Research2006，1，7： | 1 |
| 15 | Newdamage-locating method for bridges subjected to amoving load显示文摘 | Fushun L Huajun L Guangming Y | 2007 | Periodical of University of China2007，6，19： | 1 |
| 16 | Spatio-temporal expression of inducible nitric oxide synthase in and surrounding a region of rat frontal lobe damaged with a sharp instrument显示文摘BACKGROUND:Inducible nitric oxide synthase(iNOS) cannot be detected in the neurons and glial cells of normal rats,but iNOS can be found in some neurons and glial cells of rats following ischemic,traumatic,neurotoxic or inflammatory damage. OBJECTIVE:To investigate iNOS expression and iNOS-positive cell types at various time points following damage to the rat frontal lobe using a sharp instrument. DESIGN:A nerve molecular biology,randomized,controlled study. TIME AND SETTING:This experiment was performed at the Department of Human Anatomy, Institute of Neurobiology,Medical School of Nantong University,between April 2006 and December 2007. MATERIALS:Rabbit anti-iNOS antibody(Santa Cruz,USA),biotin labeled goat anti-rabbit antibody(Sigma,USA),reverse transcription kit(Biouniquer,Hong Kong,China) and horseradish peroxidase labeled goat anti-rabbit antibody(Pierce,USA) were used for this study. METHODS:A total of 112 healthy rats aged 3 months were randomly assigned into a sham operation group(n=28) and a damage group(n=84).Rat models of frontal lobe damage were induced in the damage group using a sharp instrument to make an incision in the frontal lobe cortex. In the sham operation group,the rat bone window was opened but brain tissues were left intact. MAIN OUTCOME MEASURES:Parameters were measured at 3,6,12,24,72,120 and 168 hours following damage in both groups.Pathological changes were observed using Nissl staining and hematoxylin-eosin staining.Expression of iNOS mRNA,iNOS protein and iNOS-positive cells were examined by RT-PCR,Western blot analysis and immunohistochemistry,respectively. RESULTS:A large number of inflammatory cells infiltrated the damaged region 12 and 24 hours following damage.iNOS mRNA and iNOS protein expression increased in and around the damaged region 3 hours following damage,reached a peak at 24 hours,and then gradually decreased.The changes in iNOS-positive cell number reflected the changes in iNOS mRNA and iNOS protein expression after damage.iNOS was mainly found in neural cells at 3 and 6 hours,in macrophages at 12 and 24 hours,and in glial cells at 72 and 120 hours after damage. iNOS-positive cells were few in and surrounding the damaged region at 168 hours.There were a few iNOS-positive neural cells in the rat frontal lobe cortex in the sham operation group. CONCLUSION:Neurons,macrophages and glial cells can express iNOS following rat frontal lobe damage caused by a sharp instrument.The levels of iNOS expression,and the cell types expressing iNOS,change with time. | Zhixian He Zhijun Zhang Yulin Dong Guangming Lü Ting Wang Hengjian Ni | 2009 | Neural Regeneration Research2009，4，2： | 0 |
| 17 | Crustal Electrical Structure and Deep Metallogenic Potential in Northern Wuyi Area(South China),based on Magnetotelluric Data显示文摘The northern Wuyi area,which is located in the northern Wuyi metallogenic belt,has superior mineralization conditions.The Pingxiang-Guangfeng-Jiangshan-Shaoxing fault(PSF)extends across the whole region regardless of whether or how the PSF relates to the near-surface mineralization.We carried out an MT survey in the region and obtained a reliable 2D model of the crustal electrical structure to a depth of 30 km.In the resistivity model,we inferred that a continuous high conductivity belt that ranges from the shallow to deep crust is a part of the PSF.Then,we estimated the fluid content and pressure gradient to identify the deep sources of fluid as well as its pattern of motion pattern.Finally,we proposed a model for the deep metallogenic migration processes that combines geological data,fluid content data,pressure gradient data,and the subsurface resistivity model.The model analysis showed that the Jiangnan orogenic belt and the Cathaysia block formed the PSF during the process of com.The deep fluid migrated upward through the PSF to the shallow crust.Therefore,we believe that the PSF is an ore-forming fluid migration channel and that it laid the material basis for large-scale mineralization in the shallow crust. | LUO Fan Lü Qingtian ZHANG Kun YAN Jiayong Colin GFARQUHARSON ZHANG Chong FU Guangming | 2022 | Acta Geologica Sinica(English Edition)2022，96，3： | 0 |
| 18 | TWO KINDS OF VARIANT IN SHAPE MEMORY MATERIAL显示文摘In shape memory materials,that have been trained to have a two way shape memory effect(TWSM),themartensitic variants are classified into two groups,i.e.,preferentially oriented variants and self-accommodatingvariants.Applied stress may promote or constrain the transition of preferentially oriented variants and so changetransformation temperatures but has no essential effect on self-accommodating variants.According to the pointof view mentioned above,some experimental phenomena during thermocycling of a TWSM device may be ex-plained,such as the absence of synchronization between the plot of resistance change.temperature and the plotof memory strain vs.temperature. | Lin,Guangming Lai,J L Huan,Yuanshi Zhongshan University,Guangzhou 510275,China | 1993 | 中国有色金属学会会刊:英文版1993，3，2： | 0 |
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