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| 1 | Identification of Salmonella and Listeria monocytogenes by Near-infrared Spectroscopy and PCA显示文摘Near-infrared spectra of pathogenic bacteria (salmonella and Listeria monocytogenes ) were determined, and the spectral data were analyzed by the projection discriminant analysis based on principal component analysis (PCA). The expected results were obtained. The results showed that salmonella and L. monocytogenes could be distinguished from each other by the near-infrared spectroscopy of the whole cells, cell walls or cytoplasm. | Jiang Chuan Zhang Xiaohong Zou Xiaolong Lu Guangying Liu Yangchun | 2019 | Meteorological and Environmental Research2019，10，4： | 2 |
| 2 | Modulating effects of Astragalus polysaccharide on immune disorders via gut microbiota and the TLR4/NF-κB pathway in rats with syndrome of dampness stagnancy due to spleen deficiency显示文摘The syndrome of dampness stagnancy due to spleen deficiency(DSSD)is relatively common globally.Although the pathogenesis of DSSD remains unclear,evidence has suggested that the gut microbiota might play a significant role.Radix Astragali,used as both medicine and food,exerts the effects of tonifying spleen and qi.Astragalus polysaccharide(APS)comprises a macromolecule substance extracted from the dried root of Radix Astragali,which has many pharmacological functions.However,whether APS mitigates the immune disorders underlying the DSSD syndrome via regulating gut microbiota and the relevant mechanism remains unknown.Here,we used DSSD rats induced by high-fat and low-protein(HFLP)diet plus exhaustive swimming,and found that APS of moderate molecular weight increased the body weight gain and immune organ indexes,decreased the levels of interleukin-1β(IL-1β),IL-6,and endotoxin,and suppressed the Toll-like receptor 4/nuclear factor-κB(TLR4/NF-κB)pathway.Moreover,a total of 27 critical genera were significantly enriched according to the linear discriminant analysis effect size(LEfSe).APS increased the diversity of the gut microbiota and changed its composition,such as reducing the relative abundance of Pseudoflavonifractor and Paraprevotella,and increasing that of Parasutterella,Parabacteroides,Clostridium XIVb,Oscillibacter,Butyricicoccus,and Dorea.APS also elevated the contents of short-chain fatty acids(SCFAs).Furthermore,the correlation analysis indicated that 12 critical bacteria were related to the body weight gain and immune organ indexes.In general,our study demonstrated that APS ameliorated the immune disorders in DSSD rats via modulating their gut microbiota,especially for some bacteria involving immune and inflammatory response and SCFA production,as well as the TLR4/NF-κB pathway.This study provides an insight into the function of APS as a unique potential prebiotic through exerting systemic activities in treating DSSD. | Wenxiao ZHAO Chenchen DUAN Yanli LIU Guangying LU Qin LYU Xiumei LIU Jun ZHENG Xuelian ZHAO Shijun WANG Haijun ZHAO | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，7： | 1 |
| 3 | Stability of planar waves in reaction-diffusion system显示文摘This paper is concerned with the asymptotic stability of planar waves in reaction-diffusion system on Rn, where n 2. Under initial perturbation that decays at space infinity, the perturbed solution converges to planar waves as t →∞. The convergence is uniform in Rn. Moreover, the stability of planar waves in reaction-diffusion equations with nonlocal delays is also established by transforming the delayed equations into a non-delayed reaction-diffusion system. | LU GuangYing WANG MingXin | 2011 | Science China Mathematics2011，54，7： | 1 |
| 4 | The crystal structure of bar-headed goose hemoglobin in deoxy form: the allosteric mechanism of a hemoglobin species with high oxygen affinity 1 1 Edited by A. Klug显示文摘 | Yuhe Liang Ziqian Hua Xue Liang Qian Xu Guangying Lu | 2001 | Journal of Molecular Biology2001，，1： | 1 |
| 5 | The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution显示文摘Meiotic recombination is essential for sexual reproduction and its regulation has been extensively studied in many taxa.However,genome-wide recombination landscape has not been reported in ciliates and it remains unknown how it is affected by the unique features of ciliates:the synaptonemal complex(SC)-independent meiosis and the nuclear dimorphism.Here,we show the recombination landscape in the model ciliate Tetrahymena thermophila by analyzing single-nucleotide polymorphism datasets from 38 hybrid progeny.We detect 1021 crossover(CO)events(35.8 per meiosis),corresponding to an overall CO rate of 9.9 cM/Mb.However,gene conversion by non-crossover is rare(1.03 per meiosis)and not biased towards G or C alleles.Consistent with the reported roles of SC in CO interference,we find no obvious sign of CO interference.CO tends to occur within germ-soma common genomic regions and many of the 44 identified CO hotspots localize at the centromeric or subtelomeric regions.Gene ontology analyses show that CO hotspots are strongly associated with genes responding to environmental changes.We discuss these results with respect to how nuclear dimorphism has potentially driven the formation of the observed recombination landscape to facilitate environmental adaptation and the sharing of machinery among meiotic and somatic recombination. | Lu Fu Chen Gu Kazufumi Mochizuki Jie Xiong Wei Mia Guangying Wang | 2024 | Journal of Genetics and Genomics2024，51，3： | 0 |
| 6 | Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD–ACE2 complexes in living cells by competitive binding显示文摘To the Editor:The current coronavirus disease-19(COVID-19)pandemic spurs the development of antiviral drugs for SARS-CoV-2,as the number of patients with viral infections continues to rise globally in the context of widespread vaccination.Targeting the interaction between the receptor binding domain(RBD)of SARS-CoV-2 spike protein and the host cell ACE2 is a promising therapeutic strategy to effectively inhibit viral entry. | Lu Miao Wei Zhou Chunyu Yan Yuebin Zhang Qinglong Qiao Xuelian Zhou Yingzhu Chen Guangying Wang Zhendong Guo Jun Liu Hailong Piao Xia Pan Mengxue Yan Weijie Zhao Guohui Li Yueqing Li Zhaochao Xu | 2022 | Acta Pharmaceutica Sinica B2022，12，9： | 0 |
| 7 | Three-dimensional Structure of Rice Dwarf Virus by Electron Cryomicroscopy显示文摘The three-dimensional(3D)structure of intact rice dwarf virus(RDV)has been deter-mined to 2.6 nm resolution by 400kV electron cryomicroscopy and computer reconstructiontechniques.The intact virion of 69.8 nm in diameter was seen to be made up of an outer shellof 6.9 nm in thickness and an inner shell of 2.5 nm in thickness at a diameter of 54 nm.Theouter shell surface forms a T=13 icosahedral lattice with holes at 5-and 6-coordinated posi-tions.The 780 capsomeres at local 3-fold positions appear trimeric.The interface betweenthese two shells could be clearly delineated and the interaction was maintained by a cylindri-cal density whose upper domain connects subunits of the trimeric capsomeres by an interlock-ing mechanism.The 3D structure of inner shell was also reconstructed to 3.3 nm from100kV images of purified inner shell particles,confirming our interpretation of the multilayerstructure of intact RDV. | Lu Guangying~(①②) Z.H.Zhou~② J.Jakana~② Cai Deyou~① Chen Shengxiang~③ Wei Xincheng~① Gu Xiaocheng~① W.Chiu~② (①College of Life Sciences,Peking University,Beijing 100871,P.R.China) (②W.M.Keck Center for Computational Biology and Verna & Marts McLean Dept.of Biochem.,Baylor College of Medicine,Houston,TX 77030,USA) (③Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,P.R.China) | 1995 | High Technology Letters1995，1，1： | 0 |
| 8 | Empowering higher energy sodium-ion battery cathode by oxygen chemistry显示文摘Sodium(Na)ion batteries(SIBs)promise low-cost energy storage systems but are still restricted by insufficient energy density.Introducing oxygen(O)redox into the design of the Na-storage cathode is presently considered an effective avenue to generate extra capacity in solving the energy density bottleneck.The succeeding issues are how to overcome the irreversible electrochemical behavior accompanied by O release.Meanwhile,the O redox chemistry and subsequent structural evolution remain ambiguous so far.Here,we deliberate on the O redox mechanism in Na-storage transition metal oxides.Challenges associated with the reaction irreversibility and structural collapse are summarized by virtue of the advanced characterization techniques.Beyond that,strategies that potentially enhance the electrochemical properties of O redox and future research perspectives on exploring useable O redox cathode materials are outlined. | Guangying Wan Wendi Dou Hanye Zhu Wu Zhang Tiefeng Liu Liguang Wang Jun Lu | 2023 | Interdisciplinary Materials2023，2，3： | 0 |
| 9 | Zeolitic germanosilicate analogue to pharmacosiderite crystallized in an acidic medium显示文摘Zeolites are typically synthesized in alkaline or fluoride-containing near-neutral media.Sophisticated organic structure-directing agents have been investigated for such systems with the aim of discovering materials with unprecedented structures and properties for novel technical applications.In contrast,zeolite crystallization in strongly acidic media has yet to be explored.This study demonstrates that a zeolitic silicate phase crystallizes from acidic gels using trimethylamine as an organic additive with the composition 1 SiO_(2):0.3 TMA:0.3 HCl:0.15 HF:55 H_(2)O:(0.1-0.4)GeO_(2).This phase has an interrupted four-connected framework analog to the octahedron/tetrahedron-mixed framework of the mineral family pharmacosiderite.In comparison to the pharmacosiderite-type HK_(3)(Ge_(7)O_(16))(H_(2)O)_(4),the four GeO_(6)-octahedra forming the central[HGe_(4)O_(4)O_(12)]-cluster are replaced by four SiO_(4)-tetrahedra in a[Si_(4)O_(6)(OH)2.89]-unit in the new phase.However,the structure is distorted and may contain connectivity and point defects;thus,healing by the occasional incorporation of GeO_(6)-units is necessary.The refined unit cell has a cubic symmetry,space group P-43m(#215),with a=7.7005(1)Å.Acidic-medium synthesis is a useful way to find new zeolites that move in a fundamentally different direction from sophisticated organic structure-directing agents. | Guangying Fu Qiaolin Lang Xiaolong Liu Haonuan Zhao Yiqing Sun Lei Zhao Ahmed Omran Peng Lu Xiaobo Yang Bing Yu Valentin Valtchev | 2023 | Green Carbon2023，1，2： | 0 |
| 10 | Regulation and management of the biosecurity for synthetic biology显示文摘Synthetic biology(SynBio)is a high-profile interdiscipline combining engineering with science.As a dual-purpose discipline,SynBio is bringing large changes to many fields and providing great benefits to humans.However,due to its characteristic of complexity and uncertainty,SynBio also presents potential biosafety and biosecurity risks.Biosecurity risks refer to unauthorized access,loss,theft,misuse,diversion or intentional release.If a biosecurity accident happens,it would pose a huge threat to humans and nature.Therefore,it is crucial to establish a set of regulations and management practices for the biosecurity risks of SynBio.In this paper,we summarized the sources of the biosecurity risks of SynBio,from its research materials,products,technologies,information to Do-it-yourself synthetic biology.We reviewed and analyzed the current situation of regulation and management of biosecurity for SynBio in the international community and in China.We found that in most countries and regions,SynBio risks commonly follow the regulation and management of Genetically Modified Organisms which has loopholes if applied to the regulation for SynBio without any amendments.Here,we proposed suggestions for the Chinese-featured regulation and management of biosecurity for SynBio,including a top-to-bottom governing framework,a think-tank implementation mechanism,a Synthetic Biology Laboratory Biosecurity Manual safeguarding system,and strengthening biosecurity education on synthetic biology and self-regulation awareness among relevant personnel.Through this work,we aim to improve the standardized process of biosecurity regulation and management for SynBio in China and thereby map out a peaceful,profitable,and practical development path for synthetic biology. | Xiaomei Zeng Hailun Jiang Guangying Yang Yakun Ou Shan Lu Jia Jiang Ruipeng Lei Li Su | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 0 |
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