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| 1 | Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by Small Interfering RNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice显示文摘癌症侵略和转移,包含许多病理学的过程和 cytophysiological 变化,贡献肺癌症的高死亡。类型 1insulin-like 生长因素受体(IGF-1R ) ,把前进和侵略与癌症联系了,是在肺癌症的一个潜在的反侵略和反转移目标。禁止肺癌症房间的侵略性质,我们在由小介入 RNA ( siRNA )技术的 A549 人的肺癌症房间的成功地下面调整的 IGF-1R 基因表达,并且评估了它的效果oninvasion相关的基因表达,在 vitro 侵略的肿瘤房间,并且在有为 IGF-1R 表示发卡 siRNA 的一个原生质标志的异种皮移植 nudemice.A549 房间 transfected 的转移在 mRNA 水平以及蛋白质 level.Inbiological 试金显示出一个显著地减少的 IGF-1R 表达式, transfected A549 房间显示出 cell-matrixadhesion ,移植和侵略的重要减小。与这些结果一致,我们发现那条下面规定大减小附随地在侵略相关的基因表达伴随的 ofIGR-1R , includingMMP-2 , MMP-9 , u-PA ,并且为 IGF-1R 的 plasmidexpressing 发卡 siRNA 的 IGF-1R 特定的下游的 p-Akt.Direct 尾巴静脉注射显著地在结果显示出的 nudemice.Our 禁止了肺转移的形成是的 siRNA 的治疗学的潜力为在禁止肺癌症侵略和转移的基因治疗的一个方法。 | Jianfang QIAN~1 Aiqiang DONG~1 Minjian KONG~1 Zhiyuan MA~2 Junqiang FAN~1 and Guanyu JIANG~3 ~1Department of Cardiothoracic Surgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China ~2Department of Cardiovascular Surgery,Shanghai Jiaotong University Affiliated First People’s Hospital,Shanghai 200080,China ~3Department of Emergency,Second Affiliated Hospital School of Medicine,Zhejiang University,Hangzhou 310009,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 9 |
| 2 | Targeting BMI-1-mediated epitheliale-mesenchymal transition to inhibit colorectal cancer liver metastasis显示文摘Liver is the most common metastatic site for colorectal cancer(CRC),there is no satisfied approach to treat CRC liver metastasis(CRCLM).Here,we investigated the role of a polycomb protein BMI-1 in CRCLM.Immunohistochemical analysis showed that BMI-1 expression in liver metastases was upregulated and associated with T4 stage,invasion depth and right-sided primary tumor.Knockdown BMI-1 in high metastatic HCT116 and LOVO cells repressed the migratory/invasive phenotype and reversed epithelialemesenchymal transition(EMT),while BMI-1 overexpression in low metastatic Ls174 T and DLD1 cells enhanced invasiveness and EMT.The effects of BMI-1 in CRC cells were related to upregulating snail via AKT/GSK-3βpathway.Furthermore,knockdown BMI-1 in HCT116 and LOVO cells reduced CRCLM using experimental liver metastasis mice model.Meanwhile,BMI-1 overexpression in Ls174 T and DLD1 significantly increased CRCLM.Moreover,sodium butyrate,a histone deacetylase and BMI-1 inhibitor,reduced HCT116 and LOVO liver metastasis in immunodeficient mice.Our results suggest that BMI-1 is a major regulator of CRCLM and provide a potent molecular target for CRCLM treatment. | Zhiyao Xu Zhuha Zhou Jing Zhang Feichao Xuan Mengjing Fan Difan Zhou Zhenyu Liuyang Ximei Ma Yiyang Hong Yihong Wang Sherven Sharma Qinghua Dong Guanyu Wang | 2021 | Acta Pharmaceutica Sinica B2021，11，5： | 8 |
| 3 | Iso-UniK:lightweight multi-process unikernel through memory protection keys显示文摘Unikernel,specializing a minimalistic libOS with an application,is an attractive design for cloud computing.However,the Achilles’heel of unikernel is the lack of multi-process support,which makes it less flexible and applicable.Many applications rely on the process abstraction to isolate different components.For example,Apache with the multi-processing module isolates a request handler in a process to guarantee security.Prior art tackles the problem by simulating multi-process with multiple unikernels,which is incompatible with existing cloud providers and also introduces high overhead.This paper proposes Iso-UniK,a new unikernel design enabling multi-task applications with the support of both functionality and isolation.Iso-UniK leverages a recent hardware feature,named Intel Memory Protection Key(Intel MPK),to provide lightweight and efficient isolation for multi-process in unikernel.Our design has three benefits compared with previous approaches.First,Iso-UniK does not need hypervisor support and is thus compatible with existing cloud computing platforms;second,Iso-UniK promises fast system calls with only 45 cycles;last,a process can be isolated with a flexible configuration.We have implemented a prototype based on OSv,a unikernel system supporting unmodified applications.Iso-UniK can achieve fast fork operation with only 66μs for multi-process applications.Our evaluation shows that the isolation and multi-process support in Iso-UniK will not damage the applications’performance. | Guanyu Li Dong Du Yubin Xia | 2018 | Cybersecurity2018，1，1： | 0 |
| 4 | Emerging drug design strategies in anti-influenza drug discovery显示文摘Influenza is an acute respiratory infection caused by influenza viruses(IFV),According to the World Health Organization(WHO),seasonal IFV epidemics result in approximately 3-5 million cases of severe illness,leading to about half a million deaths worldwide,along with severe economic losses and social burdens.Unfortunately,frequent mutations in IFV lead to a certain lag in vaccine development as well as resistance to existing antiviral drugs.Therefore,it is of great importance to develop anti-IFV drugs with high efficiency against wild-type and resistant strains,needed in the fight against current and future outbreaks caused by different IFV strains.In this review,we summarize general strategies used for the discovery and development of antiviral agents targeting multiple IFV strains(including those resistant to available drugs).Structure-based drug design,mechanism-based drug design,multivalent interaction-based drug design and drug repurposing are amongst the most relevant strategies that provide a framework for the development of antiviral drugs targeting IFV. | Chuanfeng Liu Lide Hu Guanyu Dong Ying Zhang Edeildo Ferreira da Silva-Jinior Xinyong Liu Luis Menendez-Arias Peng Zhan | 2023 | Acta Pharmaceutica Sinica B2023，13，12： | 0 |
| 5 | Iso-UniK:lightweight multi-process unikernel through memory protection keys显示文摘Unikernel,specializing a minimalistic libOS with an application,is an attractive design for cloud computing.However,the Achilles’heel of unikernel is the lack of multi-process support,which makes it less flexible and applicable.Many applications rely on the process abstraction to isolate different components.For example,Apache with the multi-processing module isolates a request handler in a process to guarantee security.Prior art tackles the problem by simulating multi-process with multiple unikernels,which is incompatible with existing cloud providers and also introduces high overhead.This paper proposes Iso-UniK,a new unikernel design enabling multi-task applications with the support of both functionality and isolation.Iso-UniK leverages a recent hardware feature,named Intel Memory Protection Key(Intel MPK),to provide lightweight and efficient isolation for multi-process in unikernel.Our design has three benefits compared with previous approaches.First,Iso-UniK does not need hypervisor support and is thus compatible with existing cloud computing platforms;second,Iso-UniK promises fast system calls with only 45 cycles;last,a process can be isolated with a flexible configuration.We have implemented a prototype based on OSv,a unikernel system supporting unmodified applications.Iso-UniK can achieve fast fork operation with only 66μs for multi-process applications.Our evaluation shows that the isolation and multi-process support in Iso-UniK will not damage the applications’performance. | Guanyu Li Dong Du Yubin Xia | 2020 | Cybersecurity2020，3，1： | 0 |
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