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| 1 | Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals. | YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang | 2006 | Chinese Science Bulletin2006，51，14： | 4 |
| 2 | 0.5981μm Sum Frequency Mixing in KTP Crystal显示文摘Based on accurate values of refractive indices,the phase matching angles(θ_(m))and effective nonlinear coeficients(d_(eff))for type I and I I sum frequency mixing of 1.0795 and 1.3414μm radiations were calculated in KTP crystal.The optimum phase matching conditions are thatθm=75.74o in the XZ plane(Ф=0)and deff=17.42 × 10^(-9) esu.The calculated results agree well with the experimental results.Thus,the cw 0.5981μm sum frequency radiation was achieved in KTP crystal using a 1.0795 and 1.3414μm double-wavelength Nd:YAP cw laser. | SHEN Hongyuan ZHOU Yuping LIN Wenxiong ZENG Ruirong YU Guifang HUANG Chenghui ZENG Zhengdong ZHANG Weijia JIA Shouquan JIANG Peizhi XIAO Chaoliang | 1991 | Chinese Physics Letters1991，8，4： | 2 |
| 3 | Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial–mesenchymal transition显示文摘 | Bin Zhang Yan Yang Xiaoting Shi Wanyu Liao Min Chen Alfred Sze-Lok Cheng Hongli Yan Cheng Fang Shu Zhang Guifang Xu Shanshan Shen Shuling Huang Guangxia Chen Ying Lv Tingsheng Ling Xiaoqi Zhang Lei Wang Yuzheng Zhuge Xiaoping Zou | 2014 | Cancer Letters2014，，： | 2 |
| 4 | Effect of Crystallinity Level on the Double Yielding Behavior of Polyamide 6显示文摘 | Guifang Shen Wei Yang Mingbo Yang | 2006 | Polymer Testing2006，25，4： | 1 |
| 5 | Molecular phylogeny of the domesticated silkworm,Bombyx mori, based on the sequences of mitochondrialcytochrome b genes显示文摘 | Ailing Li Qiaoling Zhao Shunming Tang Zhifang Zhang Shenyuan Pan Guifang Shen | 2005 | Journal of Genetics2005，，2： | 1 |
| 6 | Clinical and genetic analysis of two Chinese families with benign familial neonatal convulsions显示文摘Benign familial neonatal convulsions (BFNC) is a rare autosomal dominant inherited epilepsy syndrome. Two voltage-gated potassium channel genes, KCNQ2 and KCNQ3, have been identified as the genes responsible for BFNC. Here we report two Chinese families with clinical histories of typical BFNC. Using six microsatellite markers, two located at KCNQ2 locus and four at KCNQ3 locus, linkage analysis was performed in the two families, which excluded the linkage of BFNC to KCNQ3, but could not exclude the linkage to KCNQ2. Direct DNA sequencing of the KCNQ2 gene in the two families was performed, and two formerly unknown polymorphisms were identified, but no KCNQ2 mutation was found in the two families. Our study suggests the genetic heterogeneity in Chinese families with BFNC and proves the existence of a new gene locus for BFNC. | LI Haiyan TANG Beisha XIA Kun CAO Guifang SHEN Lu JIANG Hong PAN Qian SONG Yanmin CAI Fang | 2005 | Progress in Natural Science:Materials International2005，15，8： | 0 |
| 7 | Changes in erythrocyte membrane ATPase and plasma lipid peroxides during perioperative period显示文摘Changes in erythrocyte membrane ATPases and plasma lipid peroxides(LPO) were studiedperioperatively in 15 abdominal surgical patients under intravenous procaine-balanced anesthesia.The resultsrevealed that erythrocyte membrne Na+-K+ATPase,Mg2+ATPase and Ca2+-Mg2+ATPase activities werenot significantly changed at 60~90 min after incision as compared with 30 min before anesthesia,but weredecreased markedly at 10 rain and 24 hours after completion of operation(P<0.01).Plasma LPO wereincreased significantly by 24 hours after surgery(P<0.01) following an initially marked but transientreduction.Plasma LPO changes were not correlated with erythrocyte membrane ATPase activities,r=-0.0396,-0.0097 and-0.4383 respeetively(P>0.05).These correlations are suggested to be inducedby neuroendoerine responses after anesthesia and/or surgical trauma. | Tu Weifeng Lin Guifang Shen Jianfan Department of Anesthesiology,Jinling Hospital,Nanjing,China Department of Anesthesiology,First Affiliated Hospital of Nanjing Medical College,Nanjing,China | 1993 | 医学研究生学报1993，6，S1： | 0 |
| 8 | Feasibility of establishing model of Parkinson disease by injecting 6-hydroxydopamine at different parts of the nigrostriatal pathway in the brain of rats显示文摘BACKGROUND: Previous researches found that animal models with Parkinson disease (PD) could be established by injecting 6-hydroxydopamine (6-OHDA) into medial forebrain bundle (MFB), substantia nigra compacta (SNC) and caudate-putamen complex (CPU) of the nigrostriatal pathway. OBJECTIVE: To compare behavioral, biochemical and histological properties of these rats undergoing the 6-OHDA injections in the areas of MFB, SNC and CPU respectively. DESIGN: Controlled observational study. SETTING: Department of Neurology, First Affiliated Hospital of Guangxi Medical University. MATERIALS: A total of 64 adult female SD rats weighing 180-230 g were provided by the Animal Experimental Center of Guangxi Medical University. 6-OHDA (Sigma Company, USA); Brain solid positioner (Standard model 51600, Stoelting Co., IL, USA); rotational monitoring of little animal (type QL-1, USA); high liquid chromatography (HLC, Waters Company). METHODS: The experiment was carried out in the Medical Experimental Center of Guangxi Medical University from February to December 2005. ① According to digital table, 64 SD rats were divided into MFB group, SNC group, CPU group and control group with 16 in each group. On the basis of the brain atlas of Paxinos, rats in the first three groups were injected with 5 μL 6-OHDA into right MFB (0 mm of line of incisor tooth, A/P 4.4 mm, L/R 1.2 mm, O/V -7.8 mm), SNC (line of incisor tooth just equal to horizon, A/P -4.8 mm, L/R 1.6 mm, O/V -7.8 mm) and CPU (0 mm of line of incisor tooth, A/P 1.2 mm, L/R 2.7 mm, O/V -5.4 mm), respectively. The rats in control group were injected with 5 μL ascorbic acid solution (2 g/L). One week after operation, 0.1 g/L apomorphine (Apo, 0.05 mg/kg) was subcutaneously injected into neck and then rotational behavior induced by Apo was recorded once a week for 8 weeks. The PD models were considered successful only when rotational times more than or equal to 7 times per minute. ② Eight weeks after operation, micro-perfusion was used to obtain micro-perfusate in bilateral CPU and contents of 3,4-dihydroxyphenylacetic acid (3,4-DOPAC) and homovanillic acid (HVA) were also measured. In addition, amount of tyrosine hydroxylase positive cells (TH+) in SNC was counted with immunohistochemical staining. MAIN OUTCOME MEASURES: ① Successful rate of PD models; ② contents of dopamine and its metabolite in MFB, SNC and CPU groups and TH+ amount. RESULTS: All 64 SD rats were involved in the final analysis. ① Successful rate and rotational behavior: One week after operation, there were 6 successful models both in SNC and MFB groups; in the 2nd week, there were 6 both in SNC and MFB groups and 1 in CPU group; in the 3rd week, there were 1 in MFB group and 3 in CPU group; in the 4th week, there were 3 in CPU group. Otherwise, no successful case was found out in the next 3 weeks. Abnormal rotational behavior was not observed in control group. Four weeks after operation, successful rates were 81% (13/16) in MFB group, 75% (12/16) in SNC group and 44% (7/16) in CPU group. ② Contents of 3, 4-DOPAC and HVA: Eight weeks after operation, contents in the SNC area of the injured side were lower than those on non-lesion side (P < 0.01). ③ Changes of TH+ amount: Eight weeks after operation, TH+ amount in the SNC area of the lesion side was lower than that on non-lesion side (P < 0.01). CONCLUSION: Injecting 6-OHDA into MFB, SNC and CPU can damage dopaminergic cells and establish successful PD models. | Yuefei Shen Xuean Mo Guifang Long | 2006 | Neural Regeneration Research2006，1，2： | 0 |
| 9 | Establishment of Chlamydomonas reinhardtii chloroplast expression显示文摘An expression vector pACⅢ containing the chimeric gene HAV- VP3P1 and HCV-C gene has been constructed and transferred to C. reinhardtii by the biolistic method. The trans-formants have been identified by PCR, Southern-blotting, Northern-blotting and Western-blotting assays after selecting on resistant medium and incubating in the dark. The results | Guochang Fan Ning Su Zhonglin Zhang Xiangfu Wu Guifang Shen | 1999 | Chinese Science Bulletin1999，44，18： | 0 |
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