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| 1 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 2 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 3 | Methylation Modifications in Eukaryotic Messenger RNA显示文摘RNA methylation modifications have been found for decades of years, which occur at different RNA types of numerous species, and their distribution is species-specific. However, people rarely know their biological functions. There are several identified methylation modifications in eukaryotic messenger RNA(mRNA), such as N7-methylguanosine(m7G) at the cap, N6-methyl-20-O-methyladenosine(m6Am), 20-O-methylation(Nm) within the cap and the internal positions, and internal N6-methyladenosine(m6A) and 5-methylcytosine(m5C). Among them, m7G cap was studied more clearly and found to have vital roles in several important mRNA processes like mRNA translation, stability and nuclear export. m6A as the most abundant modification in mRNA was found in the 1970s and has been proposed to function in mRNA splicing, translation, stability, transport and so on. m6A has been discovered as the first RNA reversible modification which is demethylated directly by human fat mass and obesity associated protein(FTO) and its homolog protein, alkylation repair homolog 5(ALKBH5). FTO has a special demethylation mechanism that demethylases m6A to A through two over-oxidative intermediate states: N6-hydroxymethyladenosine(hm6A) and N6-formyladenosine(f6A). The two newly discovered m6A demethylases, FTO and ALKBH5, significantly control energy homeostasis and spermatogenesis, respectively, indicating that the dynamic and reversible m6A,analogous to DNA and histone modifications, plays broad roles in biological kingdoms and brings us an emerging field 'RNA Epigenetics'. 5-methylcytosine(5mC) as an epigenetic mark in DNA has been studied widely, but m5C in mRNA is seldom explored. The bisulfide sequencing showed m5C is another abundant modification in mRNA, suggesting that it might be another RNA epigenetic mark.This review focuses on the main methylation modifications in mRNA to describe their formation, distribution, function and demethylation from the current knowledge and to provide future perspectives on functional studies. | Jun Liu Guifang Jia | 2014 | Journal of Genetics and Genomics2014，41，1： | 13 |
| 4 | Segmentation of Hematoxylin-Eosin stained breast cancer histopathological images based on pixel-wise SVM classifier显示文摘Hematoxylin-Eosin(HE) staining is the routine diagnostic method for breast cancer(BC), and large amounts of HE stained histopathological images are available for analysis. It is emergent to develop computational methods to efficiently and objectively analyze these images, with the aim of providing potentially better diagnostic and prognostic information for BC. This work focuses on analyzing our in-house HE stained histopathological images of breast cancer tissues. Since tumor nests(TNs) and stroma morphological characteristics can reflect the biological behaviors of breast invasive ductal carcinoma(IDC), accurate segmentation of TNs and the stroma is the first step towards the subsequent quantitative analysis. We first propose a method based on the pixel-wise support vector machine(SVM) classifier for segmenting TNs and the stroma, then extract four morphological characters related to the TNs from the images and investigate their relationships with the patients' 8-year disease free survival(8-DFS). The evaluation result shows that the classification based segmentation method is able to distinguish between TNs and stroma with 87.1% accuracy and 80.2% precision,suggesting that the proposed method is promising in segmenting HE stained IDC histopathological images. The Kaplan-Meier survival curves show that three morphological characters(number of TNs, total perimeter, and average area of TNs) in the images have statistical correlations with 8-DFS of the patients, illustrating that the segmented images can help to identify new morphological factors in IDC TNs for the prediction of BC prognosis. | QU AiPing CHEN JiaMei WANG LinWei YUAN JingPing YANG Fang XIANG QingMing MASKEY Ninu YANG GuiFang LIU Juan LI Yan | 2015 | Science China(Information Sciences)2015，58，9： | 12 |
| 5 | Arab/c/opste N^(6)-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies显示文摘The biological functions of the epitranscriptomic modification N^(6)-methyladenosine(m^(6)A)in plants are not fully understood.CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m^(6)A-binding YTH domain.Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m^(6)A-binding function in alternative polyadenylation.Here,we charac-terized CPSF30-L as an Arabidopsis m^(6)A reader whose m^(6)A-binding function is required for the floral tran-sition and abscisic acid(ABA)response.We found that the m^(6)A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies,where CPSF30-L mainly recognizes m*A-modified far-upstream elements to control polyadenylation site choice.Deficiency of CPSF30-L lengthens the 3'untranslated region of three phenotypes-related transcripts,thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity.Collectively,this study uncovers a new molecular mechanism for m^(6)A-driven phase separation and polyadenylation in plants. | Peizhe Song Junbo Yang Chunling Wang Qiang Lu Linqing Shi Subiding Tayier Guifang Jia | 2021 | Molecular Plant2021，14，4： | 12 |
| 6 | Effects of Laser Shock Processing on Mechanical Properties of Laser Welded ANSI 304 Stainless Steel Joint显示文摘With the rapid development of engineering component with integration,high-speed and multi-parameter,traditional techniques haven't met practical needs in extreme service environment.Laser welding,a new welding technology,has been widely used.However,it would generate the drop of mechanical properties for laser welded joint due to its thermal effect.Laser shock processing(LSP) is one of the most effective methods to improve the mechanical properties of laser welded ANSI 304 stainless steel joint.In this paper,the effects of LSP on the mechanical properties of laser welded ANSI 304 stainless steel joint have been investigated.The welded joint on the front of the tensile samples is treated by LSP impacts,and the overlapping rate of the laser spot is 50%.The tensile test of the laser welded joint with and without LSP impacts is carried out,and the fracture morphology of the tensile samples is analyzed by scanning electron microscope(SEM).Compared with the yield strength of 11.70 kN,the tensile strength of 37.66 kN,the yield-to-tensile strength ratio of 0.310 7,the elongation of 25.20%,the area reduction of 32.68% and the elastic modulus of 13 063.876 MPa,the corresponding values after LSP impacts are 14.25 kN,38.74 kN,0.367 8,26.58%,42.29% and 14 754.394 MPa,respectively.Through LSP impacts,the increasing ratio of the yield strength and tensile strength are 121.79% and 102.87%,respectively;the elongation and area reduction are improved by 5.48% and 29.38%,respectively.By comparing with coarse fracture surface of the welded joint,the delamination splitting with some cracks in the sharp corner of the welded joint and asymmetric dimples,LSP can cause brighter fracture surface,and finer and more uniform dimples.Finally,the schematic illustration of dimple formation with LSP is clearly described.The proposed research ensures that the LSP technology can clearly improve the yield strength,tensile strength,yield-to-tensile strength ratio,elongation,area reduction and elastic modulus of the welded joint.The enhancement mechanism of LSP on laser welded ANSI 304 stainless steel joint is mainly due to the fact that the refined and uniform dimples effectively delay the fracture of laser welded joints. | ZHANG Yongkang ZHANG Lei LUO Kaiyu SUN Guifang LU Jinzhong DAI Fengze ZHONG Junwei | 2012 | Chinese Journal of Mechanical Engineering2012，25，2： | 11 |
| 7 | Inflammation and tumor progression:signaling pathways and targeted intervention显示文摘Cancer development and its response to therapy are regulated by inflammation,which either promotes or suppresses tumor progression,potentially displaying opposing effects on therapeutic outcomes.Chronic inflammation facilitates tumor progression and treatment resistance,whereas induction of acute inflammatory reactions often stimulates the maturation of dendritic cells(DCs)and antigen presentation,leading to anti-tumor immune responses.In addition,multiple signaling pathways,such as nuclear factor kappa B(NF-kB),Janus kinase/signal transducers and activators of transcription(JAK-STAT),toll-like receptor(TLR)pathways,cGAS/STING,and mitogen-activated protein kinase(MAPK);inflammatory factors,including cytokines(e.g.,interleukin(IL),interferon(IFN),and tumor necrosis factor(TNF)-a),chemokines(e.g.,C-C motif chemokine ligands(CCLs)and C-X-C motif chemokine ligands(CXCLs)),growth factors(e.g.,vascular endothelial growth factor(VEGF),transforming growth factor(TGF)-P),and inflammasome;as well as inflammatory metabolites including prostaglandins,leukotrienes,thromboxane,and specialized proresolving mediators(SPM),have been identified as pivotal regulators of the initiation and resolution of inflammation.Nowadays,local irradiation,recombinant cytokines,neutralizing antibodies,small-molecule inhibitors,DC vaccines,oncolytic viruses,TLR agonists,and SPM have been developed to specifically modulate inflammation in cancer therapy,with some of these factors already undergoing clinical trials.Herein,we discuss the initiation and resolution of inflammation,the crosstalk between tumor development and inflammatory processes.We also highlight potential targets for harnessing inflammation in the treatment of cancer. | Huakan Zhao Lei Wu Guifang Yan Yu Chen Mingyue Zhou Yongzhong Wu Yongsheng Li | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 11 |
| 8 | Measurement Uncertainty Evaluation of Conicity Error Inspected on CMM显示文摘The cone is widely used in mechanical design for rotation, centering and fixing. Whether the conicity error can be measured and evaluated accurately will directly influence its assembly accuracy and working performance. According to the new generation geometrical product specification(GPS), the error and its measurement uncertainty should be evaluated together. The mathematical model of the minimum zone conicity error is established and an improved immune evolutionary algorithm(IIEA) is proposed to search for the conicity error. In the IIEA, initial antibodies are firstly generated by using quasi-random sequences and two kinds of affinities are calculated. Then, each antibody clone is generated and they are self-adaptively mutated so as to maintain diversity. Similar antibody is suppressed and new random antibody is generated. Because the mathematical model of conicity error is strongly nonlinear and the input quantities are not independent, it is difficult to use Guide to the expression of uncertainty in the measurement(GUM) method to evaluate measurement uncertainty. Adaptive Monte Carlo method(AMCM) is proposed to estimate measurement uncertainty in which the number of Monte Carlo trials is selected adaptively and the quality of the numerical results is directly controlled. The cone parts was machined on lathe CK6140 and measured on Miracle NC 454 Coordinate Measuring Machine(CMM). The experiment results confirm that the proposed method not only can search for the approximate solution of the minimum zone conicity error(MZCE) rapidly and precisely, but also can evaluate measurement uncertainty and give control variables with an expected numerical tolerance. The conicity errors computed by the proposed method are 20%-40% less than those computed by NC454 CMM software and the evaluation accuracy improves significantly. | WANG Dongxia SONG Aiguo WEN Xiulan XU Youxiong QIAO Guifang | 2016 | Chinese Journal of Mechanical Engineering2016，29，1： | 10 |
| 9 | Ground Surface Ruptures and Near-Fault,Large-Scale Displacements Caused by the Wenchuan Ms8.0 Earthquake Derived from Pixel Offset Tracking on Synthetic Aperture Radar Images显示文摘The 12 May 2008 Wenchuan Ms8.0 earthquake produced surface displacements along the causative fault,the Yingxiu-Beichuan Fault,which are up to several meters near the fault.Because of the large gradient,satellite synthetic aperture radar(SAR) interferometric data are strongly incoherent;the usual SAR interferometry method does not allow such displacements to be measured.In the present study,we employed another approach,the technique based on pixel offset tracking,to solve this problem.The used image data of six tracks are from the Advanced Land Observing Satellite,Phased Array type L-band Synthetic Aperture Radar(ALOS/PALSAR) dataset of Japan.The results show that the entire surface rupture belt is 238 km long,extending almost linearly in a direction of 42° north-east.It is offset left laterally by a north-west-striking fault at Xiaoyudong,and turns at Gaochuan,where the rupture belt shifts toward the south by 5 km,largely keeping the original trend.In terms of the features of the rupture traces,the rupture belt can be divided into five sections and three types.Among them,the Beichuan-Chaping and Hongkou-Yingxiu sections are relatively complex,with large widths and variable traces along the trend.The Pingtong-Nanba and Qingping-Jingtang sections appear uniform,characterized by straight traces and small widths.West of Yingxiu,the rupture traces are not clear.North of the rupture belt,surface displacements are 2.95 m on average,mostly 2-3.5 m,with 7-9 m the maximum near Beichuan.South of the rupture belt,the average displacement is 1.75 m,dominated by 1-2 m,with 3-4 m at a few sites.In the north,the displacements in the radar line of sight are of subsidence,and in the south,they are uplifted,in accordance with a right-slip motion that moves the northern wall of the fault to the east,and the southern wall to the west,respectively.Along the Guanxian-Jiangyou Fault,there is a uplift zone in the radar line of sight,which is 66 km long,1.5-6 km wide,and has vertical displacements of approximately 2 m,but no observable rupture traces. | QU Chunyan SHAN Xinjian LIU Yunhua ZHANG Guohong SONG Xiaogang ZHANG Guifang GUO Liming HAN Yufei | 2012 | Acta Geologica Sinica(English Edition)2012，86，2： | 10 |
| 10 | A Transgenic Study on Affecting Potato Tuber Yield by Expressing the Rice Sucrose Transporter Genes OsSUT5Z and OsSUT2M显示文摘在许多植物,蔗糖 transporters 为从来源和进口的两个蔗糖出口是必要的进水池,显示功能在吸收划分。为了调查蔗糖 transporters 是否能改进淀粉的收益,种,土豆植物(茄属 tuberosum L。cv。 | Aijun Sun Yan Dai Xinsheng Zhang Chunmin Li Kun Meng Honglin Xu Xiaoli Wei Guifang Xiao Pieter B.F, Ouwerkerk Mei Wang Zhen Zhu | 2011 | Journal of Integrative Plant Biology2011，53，7： | 9 |
| 11 | Reversible RNA Modification N^1-methyladenosine(m^1A) in mRNA and tRNA显示文摘More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N^6-methyladenosine(m^6A), N^1-methyladenosine(m^1A) has been found as a reversible modification in tRNA and mRNA. m^1A occurs at positions 9, 14, and 58 of tRNA, with m^1A58 being critical for t RNA stability. Other than the hundreds of m^1A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m^1A also identifies>20 m^1A sites in mitochondrial genes. m^1A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of m^1A in mRNA and tRNA, covering high-throughput sequencing methods developed for m^1A methylome, m^1A-related enzymes(writers and erasers), as well as its functions in mRNA and tRNA. | Chi Zhang Guifang Jia | 2018 | Genomics, Proteomics & Bioinformatics2018，16，3： | 8 |
| 12 | MiRNA-203 suppresses tumor cell proliferation, migration and invasion by targeting Slug in gastric cancer显示文摘 | Liuqing Yang Hongwei Liang Yanbo Wang Shanting Gao Kai Yin Zhijian Liu Xi Zheng Ying Lv Lei Wang Chen-Yu Zhang Xi Chen Guifang Xu Weijie Zhang Xiaoping Zou | 2016 | Protein & Cell2016，7，5： | 7 |
| 13 | More than Carbon Stocks:A Case Study of Ecosystem-based Benefits of REDD+ in Indonesia显示文摘During the 15th Conference of the Parties(COP 15),Parties agreed that reducing emissions from deforestation and forest degradation and enhancing 'removals of greenhouse gas emission by forests'(REDD+) in developing countries through positive incentives under the United Nations Framework Convention on Climate Change(UNFCCC) was capable of dealing with global emissions.As REDD+ seeks to lower emissions by stopping deforestation and forest degradation with an international payment tier according to baseline scenarios,opportunities for ecosystem benefits such as slowing habitat fragmentation,conservation of forest biodiversity,soil conservation may be also part of this effort.The primary objective of this study is to evaluate ecosystem-based benefits of REDD+,and to identify the relationships with carbon stock changes.To achieve this goal,high resolution satellite images are combined with Normalized Difference Vegetation Index(NDVI) to identify historical deforestation in study area of Central Kalimantan,Indonesia.The carbon emissions for the period of 2000-2005 and 2005-2009 are 2.73 × 10 5 t CO 2 and 1.47 × 10 6 t CO 2 respectively,showing an increasing trend in recent years.Dring 2005-2009,number of patches(NP),patch density(PD),mean shape index distribution(SHAPE_MN) increased 30.8%,30.7% and 7.6%.Meanwhile,largest patch index(LPI),mean area(AREA_MN),area-weighted mean of shape index distribution(SHAPE_AM),neighbor distance(ENN_MN) and interspersion and juxtaposition index(IJI) decreased by 55.3%,29.7%,15.8%,53.4% and 21.5% respectively.The area regarding as positive correlation between carbon emissions and soil erosion was approximately 8.9 × 10 3 ha corresponding to 96.0% of the changing forest.These results support the view that there are strong synergies among carbon loss,forest fragmentation and soil erosion in tropical forests.Such mechanism of REDD+ is likely to present opportunities for multiple benefits that fall outside the scope of carbon stocks. | LU Heli YAN Weiyang QINYaochen LIU Guifang | 2012 | Chinese Geographical Science2012，22，4： | 6 |
| 14 | A phaseⅠtrial of an oral subtype-selective histone deacetylase inhibitor,chidamide,in combination with paclitaxel and carboplatin in patients with advanced non-small cell lung cancer显示文摘Objective: This phase Ⅰ study was to evaluate safety, maximum tolerated dose, pharmacokinetics and preliminary antitumor activity of chidamide, a novel subtype-selective histone deacetylase(HDAC) inhibitor, in combination with paclitaxel and carboplatin in patients with advanced non-small cell lung cancer(NSCLC).Methods: Ten patients received oral chidamide 20, 25, or 30 mg twice per week continuously with paclitaxel(175 mg/m^2) and carboplatin [area under the curve(AUC) 5 mg/m L/min] administered in a 3-week cycle. Patients with response and stable disease after four cycles maintained chidamide monotherapy until disease progression or unacceptable toxicity. Blood samples were collected for pharmacokinetic analysis after the first single oral of chidamide and first combination treatment in cycle 1 from all patients.Results: Two dose-limiting toxicities were recorded in the 30 mg cohort, including thrombocytopenia and prolonged neutropenia in the first cycle. Grade 3/4 neutropenia in any cycle was observed in all patients, but was not associated with significant complications. Other grade 3/4 hematologic toxicities included thrombocytopenia and leucopenia. No significant changes were observed in pharmacokinetic parameters for both chidamide and paclitaxel. One patient in the 20 mg cohort had confirmed partial response(PR). Two out of 5 patients with brain metastases had intracranial complete remission after 4-cycle treatment.Conclusions: Chidamide combined with paclitaxel and carboplatin was generally tolerated without unanticipated toxicities or clinically relevant pharmacokinetic interactions. The recommended dose for chidamide in this combination was established at 20 mg, and a phase Ⅱ trial is ongoing with this regimen in patients with advanced NSCLC. | Xingsheng Hu Lin Wang Lin Lin Xiaohong Han Guifang Dou Zhiyun Meng Yuankai Shi | 2016 | Chinese Journal of Cancer Research2016，28，4： | 6 |
| 15 | A novel mechanism controls anther opening and closing in Paris polyphylla var. Yunnanensis显示文摘The phenomena of anther opening and closing in Paris polyphylla var. yunnanensis (Franch) Hand.-Mazz were described in detail, and the effects of ecological factors on those phenomena related to anther opening, closing and the fly-pollination mechanism were discussed. Anthers of P. polyphylla var. yunnanensis open in the morning and close in the evening every day during an over 20 days’ period of anthesis. Light was detected as the main factor controlling this daily anther opening and closing. Anther opening can be reversed in response to rain and re-opens when rain stops. Relative humidity (RH) has a slight effect on anther opening and closing. Flower spans can be prolonged under laboratory conditions. This novel behavior of anther is likely to be a mechanism to enhance the male fitness in this species, of which has no inherited structure to protect pollen from the perianth. | WANG DingKang SUN GuiFang WANG LiFang ZHAI ShuHua CEN XiaoJiang | 2009 | Chinese Science Bulletin2009，54，2： | 5 |
| 16 | Preparation and characteristics of flexible all-organic thin-film field-effect transistor显示文摘All-organic thin-film field-effect transistor was prepared on flexible poly(ethylene-terephthalate) (PET) substrate. Poly(methyl-methacrylate) (PMMA) and pentacene are used as a dielectric layer and a semiconductor layer, respectively. The hole mobility of the transistor can reach 2.10×10-2 cm2/Vs, and the on/off current ratio was larger than 105. The performances of the transistor, when the substrate is cured under different radius, were also measured. It was found that the device performance did not change when the curly direction was vertical to the channel length direction and when the curly direction was parallel to the channel length direction with 3.67 cm curvature radius, the mobility of the device increased by more than 20% and the on/off ratio decreased more than one order. | QIU Yong, HU Yuanchuan, Dong Guifang, WANG Liduo, Xie Junfeng & MA Yaning Organic Optoelectronic Laboratory,Department of Chemistry, Tsinghua University, Beijing 100084, China (e-mail: qiuy@mail.Tsinghua.edu.cn) | 2003 | Chinese Science Bulletin2003，48，15： | 5 |
| 17 | Application of a new feature extraction and optimization method to surface defect recognition of cold rolled strips显示文摘Considering that the surface defects of cold rolled strips are hard to be recognized by human eyes under high-speed cir- cumstances, an automatic recognition technique was discussed. Spectrum images of defects can be got by fast Fourier transform (FFT) and sum of valid pixels (SVP), and its optimized center region, which concentrates nearly all energies, are extracted as an original feature set. Using genetic algorithm to optimize the feature set, an optimized feature set with 51 features can be achieved. Using the optimized feature set as an input vector of neural networks, the recognition effects of LVQ neural networks have been studied. Experiment results show that the new method can get a higher classification rate and can settle the automatic recognition problem of surface defects on cold rolled strips ideally. | Guifang Wu Ke Xu Jinwu Xu | 2007 | Journal of University of Science and Technology Beijing2007，14，5： | 5 |
| 18 | Stem cell lineage in body layer specialization and vascular patterning of rice root and leaf显示文摘Since the first appearance of vascular plants during evolution, the plant body has become specialized for adaption to land conditions. Much of our knowledge of plant body specialization and the origins of tissues from stem cells have been obtained from studies on the dicot Arabidopsis thaliana. However, less is known about plant body specialization in monocots, another important branch of angiosperms. In this study, we analyzed stem cell lineage and differentiation during development of the root and leaf of the monocot model plant rice(Oryza sativa). Our results showed that three body layers of rice are established from stem cells accompanied by progressively reduced pluripotency. Layer 1(L1) is a single-cell layer of epidermis; L2 is the cortex/endodermis in the root and the mesophyll in the leaf; and L3 is the site of vascular initiation. At least two common steps in vascular development are shared between rice root and leaf. The preprocambium divides to form the procambium and root pericycle or leaf outer sheath. The procambium further differentiates into the xylem, phloem and circumambient cells. We found that the outer sheath of leaf vascular bundles originates not only from the preprocambium of L3,but also from the mesophyll precursor cells of L2. In addition, WUSCHEL-RELATED HOMEOBOX(WOX)genes are expressed in not only the stem cell niche but also metaxylem precursor in rice. This pattern differs from that of homologs in Arabidopsis, suggesting that WOX functions have been recruited in different stem cells in dicots and monocots. | Minhuan Zeng Bo Hu Jiqin Li Guifang Zhang Ying Ruan Hai Huang Hua Wang Lin Xu | 2016 | Science Bulletin2016，61，11： | 5 |
| 19 | Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals. | YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang | 2006 | Chinese Science Bulletin2006，51，14： | 4 |
| 20 | Underwater Laser Welding/Cladding for High-performance Repair of Marine Metal Materials:A Review显示文摘With the rapid developments of marine resource exploitation,mounts of marine engineering equipment are settled on the ocean.When it is not possible to move the damaged equipment into a dry dock,welding operations must be performed in underwater environments.The underwater laser welding/cladding technique is a promising and advanced technique which could be widely applied to the maintenance of the damaged equipment.The present review paper aims to present a critical analysis and engineering overview of the underwater laser welding/cladding technique.First,we elaborated recent advances and key issues of drainage nozzles all over the world.Next,we presented the underwater laser processing and microstructural-mechanical behavior of repaired marine materials.Then,the newly developed powder-feeding based and wire-feeding based underwater laser direct metal deposition techniques were reviewed.The differences between the convection,conduction,and the metallurgical kinetics in the melt pools during underwater laser direct metal deposition and in-air laser direct metal deposition were illustrated.After that,several challenges that need to be overcame to achieve the full potential of the underwater laser welding/cladding technique are proposed.Finally,suggestions for future directions to aid the development of underwater laser welding/cladding technology and underwater metallurgical theory are provided.The present review will not only enrich the knowledge in the underwater repair technology,but also provide important guidance for the potential applications of the technology on the marine engineering. | Guifang Sun Zhandong Wang Yi Lu Mingzhi Chen Kun Yang Zhonghua Ni | 2022 | Chinese Journal of Mechanical Engineering2022，35，1： | 4 |
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