|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses显示文摘The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins(S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81(monkey kidney), Huh-7(human liver), and PK-15(pig kidney) cells efficiently. CCL94(cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening. | Jingjing Wang Feng Deng Gang Ye Wanyu Dong Anjun Zheng Qigai He Guiqing Peng | 2016 | Virologica Sinica2016，31，1： | 2 |
| 2 | Two Inhibitors Against the 3C-Like Proteases of Swine Coronavirus and Feline Coronavirus显示文摘Coronaviruses(CoVs)are important human and animal pathogens that cause respiratory and gastrointestinal diseases.Porcine epidemic diarrhoea(PED),characterized by severe diarrhoea and vomiting in pigs,is a highly lethal disease caused by porcine epidemic diarrhoea virus(PEDV)and causes substantial losses in the swine industry worldwide.However,currently available commercial drugs have not shown great therapeutic effects.In this study,a fluorescence resonance energy transfer(FRET)-based assay was applied to screen a library containing 1,590 compounds and identified two compounds,3-(aminocarbonyl)-1-phenylpyridinium and 2,3-dichloronaphthoquinone,that target the 3C-like protease(3CL^(pro))of PEDV.These compounds are of low molecular weight(MW)and greatly inhibited the activity of this enzyme(IC_(50) values were obtained in this study).Furthermore,these compounds exhibited antiviral capacity against another member of the CoV family,feline infectious peritonitis virus(FIPV).Here,the inhibitory effects of these compounds against CoVs on Vero cells and feline kidney cells were identified(with EC_(50) values)and cell viability assays were performed.The results of putative molecular docking models indicate that these compounds,labeled compound 1 and compound 2,contact the conserved active sites(Cys144,Glu165,Gln191)of 3CL^(pro) via hydrogen bonds.These findings provide insight into the antiviral activities of compounds 1 and 2 that may facilitate future research on anti-CoV drugs. | Mengxin Zhou Yutong Han Mengxia Li Gang Ye Guiqing Peng | 2021 | Virologica Sinica2021，36，6： | 1 |
| 3 | Hole polarons in poly(G)-poly(C) and poly(A)-poly(T) DNA molecules显示文摘The polaron might play an important role in the process of charge migration through duplex DNA stack. In the present work, we investigate properties of hole polarons in DNA molecules containing identical base pairs, such as poly(G)-poly(C) and poly(A)-poly(T), An extended tight-binding model (extended Su-Schrieffer-Heeger model), which involves the effect of an electric field in the direction of DNA stack, will be introduced. The transfer integral and electron-phonon coupling parameters in this model are obtained according to ab initio calculation for different base pair dimers. Calculations reveal that the polaron in poly(A)-poly(T) has a wider shape and a higher mobility under a specific electric field than that in poly(G)-poly(C) DNA stack. | CUI Peng WU Jian ZHANG GuiQing LIU ChengBu | 2008 | Science China Chemistry2008，51，12： | 1 |
| 4 | Occurrence Regularity of Tobacco Cutworm Moth (Spodoptera Litura) in Low-Altitude Tobacco Cultivation Areas in Lincang显示文摘[Objective] The paper was to study the occurrence and damage regularity of tobacco cutworm moth (Spodoptera Litura Fabricius) in low-altitude area in Lincang city. [Method] The adult sex attractant monitoring, field investigation of occurrence and damage, and observation of continuous generation development of natural populations were carried out in natural flue-cured tobacco fields. [Result] In field growth period of flue-cured tobacco under low-altitude of 1 000-1 300 m, tobacco cutworm moth occurred for 4 generations, the duration of each generation was about 33 d, and the main injurious generation was the third generation. [Conclusion] The paper provided reference for comprehensive control of tobacco cutworm moth in the low altitude areas. | Mingwen YANG Xuebing ZHANG Ping YA Guiqing PENG Ping CHEN Jianjun YU Dongcheng ZHANG | 2012 | Plant Diseases and Pests2012，3，2： | 0 |
| 5 | Nano-bubble hydrogen water:An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model显示文摘Since the anti-inflammatory effect of hydrogen has been widely known,it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions.However,hydrogen is slightly soluble in water,which leads to poor effect of oral hydrogen-rich water therapy.In this study,the nano-bubble hydrogen water(nano-HW)(about 0.7 ppm)was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus(SVCV)-infected zebrafish as model.Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group(control group).The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40%after treatment with nano-bubble hydrogen water,and q RT-PCR results showed that SVCV replication was significantly inhibited.Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water.Futhermore,SVCV infection caused reactive oxygen species(ROS)accumulation was significantly reduced upon nano-HW treatment.The level of proinflammatory cytokines IL-1β,IL-8,and TNF-αwas remarkably reduced in the nano-HW-treated group in vivo and in vitro.Taken together,our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish,which suggests that nano-HW can be applied to antiviral research,and provides a novel therapeutic strategy for virus-caused inflammation related disease. | Chen Li Yiran Cao Fukuda Kohei Haihong Hao Guiqing Peng Can Cheng Jing Ye | 2022 | Virologica Sinica2022，37，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员