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| 1 | Virus-induced Phytoene Desaturase(PDS) Gene Silencing Using Tobacco Rattle Virus in Lilium × formolongi显示文摘Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing(VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco rattle virus(TRV)-based VIGS of a PHYTOENE DESATURASE(PDS) ortholog(LhPDS) can be achieved in Lilium × formolongi seedlings, with a survival rate of 92%, using the inoculation method of rubbing plus injection. Compared with untreated and mock-treated seedlings, the photobleached leaf phenotype of silenced plants significantly correlates with down-regulation of endogenous LhPDS(P ≤ 0.05). In addition, the silencing phenomenon can be observed in the growing points of plants, indicating that systemic viral infection was achieved using the protocol. The results indicate that TRV-based VIGS can be used to characterize gene function in Lilium × formolongi. This work lays the foundation for gene function analysis and molecular breeding in Lilium spp. | Hua Xu Leifeng Xu Panpan Yang Yuwei Cao Yuchao Tang Guoren He Suxia Yuan Jingyi Lei Jun Ming | 2019 | Horticultural Plant Journal2019，5，1： | 6 |
| 2 | Investigation on the crystalline morphologies of polyphenylene sulfide and interfacial effect in its fibre composites显示文摘 | Zeng Hanmin He Guoren | 1984 | Die Angewandte Makromolekulare Chemie1984，127，： | 1 |
| 3 | Adhesion and surface analysis of carbon fibres electrochemically oxidized in aqueous potassium nitrate显示文摘 | S. F Waseem S. D Gardner Guoren He Wenbo Jiang U Pittman | 1998 | Journal of Materials Science1998，，12： | 1 |
| 4 | A Survey of Web Information Systems and Applications显示文摘WISA 2006 has received 581 submissions and has accepted 65 papers for publication of this issue. These papers are involved in 8 research areas, including Web Information Mining and Retrieval, Semantic Web and Intelligent Web, Web Data Management and Information Integration, Web Application Framework and Architecture, Web Information Security, Web Services and Workflow Models, Text Processing and Decision Support, and Grid and Networking Technology. This paper gives an introduction to previous WISA conferences and a survey on the papers to be published in this issue. | WANG Guoren XU Lizhe XU Baowe YU Ge MENG Xiaofeng HE Yanxiang LI Ruixuan | 2006 | Wuhan University Journal of Natural Sciences2006，11，5： | 1 |
| 5 | Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Lilium spp.)显示文摘The Asiatic hybrid lily(Lilium spp.)is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns.However,the regulatory mechanism underlying lily flower color has been largely unexplored.Here,we identified a WRKY transcription factor from lily tepals,LhWRKY44,whose expression was closely associated with anthocyanin accumulation.Functional verification indicated that LhWRKY44 positively regulated anthocyanin accumulation.LhWRKY44 physically interacted with LhMYBSPLATTER and directly bound to the LhMYBSPLATTER promoter,which enhanced the effect of the LhMYBSPLATTER-LhbHLH2 MBW complex activator on anthocyanin accumulation.Moreover,EMSA and dual-luciferase assays revealed that LhWRKY44 activated and bound to the promoters of gene LhF3H and the intracellular anthocyanin-related glutathione S-transferase gene LhGST.Interestingly,our further results showed that LhWRKY44 participated in light and drought-induced anthocyanin accumulation,and improved the drought tolerance in lily via activating stress-related genes.These results generated a multifaceted regulatory mechanism for the LhWRKY44-meditaed enhancement by the environmental signal pathway of anthocyanin accumulation and expanded our understanding of the WRKY-mediated transcriptional regulatory hierarchy modulating anthocyanin accumulation in Asiatic hybrid lilies. | Mengmeng Bi Rui Liang Jiawen Wang Yuxiao Qu Xin Liu Yuwei Cao Guoren He Yue Yang Panpan Yang Leifeng Xu Jun Ming | 2023 | Horticulture Research2023，10，9： | 0 |
| 6 | The MYB transcription factor RcMYB1 plays a central role in rose anthocyanin biosynthesis显示文摘Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this study,we found that a key R2R3-MYB transcription factor,RcMYB1,plays a central role in rose anthocyanin biosynthesis.Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves.In 35S:RcMYB1 transgenic lines,a significant accumulation of anthocyanins occurred in leaves and petioles.We further identified two MBW complexes(RcMYB1-RcBHLH42-RcTTG1;RcMYB1-RcEGL1-RcTTG1)associated with anthocyanin accumulation.Yeast one-hybrid and luciferase assays showed that RcMYB1 could active its own gene promoter and those of other EBGs(early anthocyanin biosynthesis genes)and LBGs(late anthocyanin biosynthesis genes).In addition,both of the MBW complexes enhanced the transcriptional activity of RcMYB1 and LBGs.Interestingly,our results also indicate that RcMYB1 is involved in the metabolic regulation of carotenoids and volatile aroma.In summary,we found that RcMYB1 widely participates in the transcriptional regulation of ABGs(anthocyanin biosynthesis genes),indicative of its central role in the regulation of anthocyanin accumulation in rose.Our results provide a theoretical basis for the further improvement of the flower color trait in rose by breeding or genetic modification. | Guoren He Ren Zhang Shenghang Jiang Huanhuan Wang Feng Ming | 2023 | Horticulture Research2023，10，6： | 0 |
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