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| 1 | OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm显示文摘Pyruvate kinase(PK) is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice(Oryza sativa) mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpa_1, which encodes a plastidic isoform of PK(PKp). OsPKpa_1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpa_1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, atthe early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein–protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts. | Yue Cai Wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang Wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang Wang Ling Jiang William Terzaghi Chunming Wang Jianmin Wan | 2018 | Journal of Integrative Plant Biology2018，60，11： | 3 |
| 2 | Effects of dry fog humidification on pericarp browning and quality of litchi fruit stored at low temperature显示文摘Pericarp browning is the major cause of deterioration of harvested litchi fruit.Water loss plays a role in pericarp browning of litchi fruit.This study investigated the effects of humidification with dry fog on pericarp browning and quality of litchi fruit stored at low temperature.Litchi fruit were stored in a non-humidified cold chamber(control)or in a humidified cold room using Tabor atomizer system that generated 95%relative humidity(RH)without depositing water on the fruit surface at 4℃.Control fruit stored in cold room without added humidity underwent rapid weight loss,accompanied by severe pericarp browning after 25 d of storage.However,slight weight loss and no obvious pericarp browning were found in humidified-fruit.Moreover,humidification maintained well the integrity of cell membrane and inhibited polyphenol oxidase activity during early storage.In addition,respiration rate was obviously inhibited in humidified-fruit compared with control fruit.This study might provide a convenient approach to reduce pericarp browning of harvested litchi fruit by humidifying the fruit using the Tabor atomizer at low temperature instead of packaging with film. | Lu Xiao Taotao Li Guoxiang Jiang Afiya John Dandan Zhang Wenyuan Jin Xuewu Duan Yueming Jiang | 2019 | International Journal of Agricultural and Biological Engineering2019，12，4： | 2 |
| 3 | Alternative splicing of MaMYB16L regulates starch degradation in banana fruit during ripening显示文摘The alternative splicing of select genes is an important mechanism to regulate responses to endogenous and environmental signals in plants.However,the role of alternative splicing in regulating fruit ripening remains unclear.Here,we discovered that MaMYB16L,an R1-type MYB transcription factor,undergoes alternative splicing and generates two transcripts,the full-length isoform MaMYB16L and a truncated form MaMYB16S,in banana fruit.During banana fruit ripening,the alternative splicing process intensifies with downregulated MaMYB16L and upregulated MaMYB16S.Moreover,MaMYB16L is a transcriptional repressor that directly binds with the promoters of many genes associated with starch degradation and MaDREB2,a positive ripening regulator,and represses their expression.In contrast,MaMBY16S lacks a DNA-binding domain but competitively combines and forms non-functional heterodimers with functional MaMYB16L.MaMYB16L-MaMYB16S heterodimers decrease the binding capacity and transrepression activity of MaMYB16L.The downregulation of MaMYB16L and the upregulation of MaMYB16S,that is,a decreased ratio of active to non-active isoforms,facilitates the activation of ripening-related genes and thereby promotes fruit ripening.Furthermore,the transient overexpression of MaMYB16S promotes banana fruit ripening,whereas the overexpression of MaMYB16L delays this process.Therefore,the alternative splicing of MaMYB16L might generate a self-controlled regulatory loop to regulate banana fruit ripening. | Guoxiang Jiang Dandan Zhang Zhiwei Li Hanzhi Liang Rufang Deng Xinguo Su Yueming Jiang Xuewu Duan | 2021 | Journal of Integrative Plant Biology2021，63，7： | 2 |
| 4 | Novel Cardioprotective Role of a Small Heat-Shock Protein, Hsp20, Against Ischemia/Reperfusion Injury显示文摘 | Guo-Chang Fan Xiaoping Ren Jiang Qian Qunying Yuan Persoulla Nicolaou Yang Wang W Keith Jones Guoxiang Chu Evangelia G. Kranias | 2005 | Circulation2005，，14： | 1 |
| 5 | Flavonoid contents and antioxidant activities from Cinnamomum species显示文摘 | K. Nagendra Prasad Bao Yang Xinhong Dong Guoxiang Jiang Haiyan Zhang Haihui Xie Yueming Jiang | 2009 | Innovative Food Science and Emerging Technologies2009，，4： | 1 |
| 6 | Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria显示文摘Only a small proportion of patients with triple-negative breast cancer benefit from immune checkpoint inhibitor(ICI)targeting PD-1/PD-L1 signaling in combination with chemotherapy.Here,we discovered that therapeutic response to ICI plus paclitaxel was associated with subcellular redistribution of PD-L1.In our immunotherapy cohort of ICI in combination with nab-paclitaxel,tumor samples from responders showed significant distribution of PD-L1 at mitochondria,while non-responders showed increased accumulation of PD-L1 on tumor cell membrane instead of mitochondria.Our results also revealed that the distribution pattern of PD-L1 was regulated by an ATAD3A-PINK1 axis.Mechanistically,PINK1 recruited PD-L1 to mitochondria for degradation via a mitophagy pathway.Importantly,paclitaxel increased ATAD3A expression to disrupt proteostasis of PD-L1 by restraining PINK1-dependent mitophagy.Clinically,patients with tumors exhibiting high expression of ATAD3A detected before the treatment with ICI in combination with paclitaxel had markedly shorter progression-free survival compared with those with ATAD3A-low tumors.Preclinical results further demonstrated that targeting ATAD3A reset a favorable antitumor immune microenvironment and increased the efficacy of combination therapy of ICI plus paclitaxel.In summary,our results indicate that ATAD3A serves not only as a resistant factor for the combination therapy of ICI plus paclitaxel through preventing PD-L1 mitochondrial distribution,but also as a promising target for increasing the therapeutic responses to chemoimmunotherapy. | Xiao-Qing Xie Yi Yang Qiang Wang Hao-Fei Liu Xuan-Yu Fang Cheng-Long Li Yi-Zhou Jiang Shuai Wang Hong-Yu Zhao Jing-Ya Miao Shuai-Shuai Ding Xin-Dong Liu Xiao-Hong Yao Wen-Tao Yang Jun Jiang Zhi-Ming Shao Guoxiang Jin Xiu-Wu Bian | 2023 | Cell Research2023，33，3： | 1 |
| 7 | Superhydrophobic Purple Orchid Leaves:Variation in Surface Morphology During the Vegetation Stages Leading to Diversity in Wettability显示文摘Learning hydrophobic phenomena from nature is always a promising approach to design the superhydrophobic surface.Purple orchid leaf which processes superhydrophobicity is an ideal plant model,and through mimicking its structure,the surface with excellent hydrophobicity is able to be obtained.However,the unclear of the diversity in wettability during the different vegetation stages and the absence of its relation to the surface morphology limits the further enhancement of the inspired structure.Here,we analyze the wettability difference as the leaf grows from tender to mature and then to senescent.Combining with the variation of surface morphology and chemical composition,the well-developed micro-scale basic unit bumps with dense nano-scale waxy layer on the surface are proven to be responsible for the best hydrophobicity of the mature leaf.The presence of the undeveloped or damaged micro-nano hierarchical structure reduces the formation of air pockets at the interface,leading to the decrease of the wettability for leaves at other stages.Moreover,by fabricating artificial leaves,the nano-waxy layer is proved to be more effective than that of the micro-bumps on the surface wettability.The results of study are of a great significance for guiding the design and fabrication of plant-inspired bionic superhydrophobic surface. | Longsheng Lu Guoxiang Jiang Kaikai Li Yingxi Xie Jiao Gao | 2023 | Journal of Bionic Engineering2023，20，6： | 0 |
| 8 | Cutting performance of surgical electrodes by constructing bionic microstriped structures显示文摘Surgical electrodes rely on thermal effect of high-frequency current and are a widely used medical tool for cutting and coagulating biological tissue.However,tissue adhesion on the electrode surface and thermal injury to adjacent tissue are serious problems in surgery that can affect cutting performance.A bionic microstriped structure mimicking a banana leaf was constructed on the electrode via nanosecond laser surface texturing,followed by silanization treatment,to enhance lyophobicity.The effect of initial,simple grid-textured,and bionic electrodes with different wettabilities on tissue adhesion and thermal injury were investigated using horizontal and vertical cutting modes.Results showed that the bionic electrode with high lyophobicity can effectively reduce tissue adhesion mass and thermal injury depth/area compared with the initial electrode.The formation mechanism of adhered tissue was discussed in terms of morphological features,and the potential mechanism for antiadhesion and heat dissipation of the bionic electrode was revealed.Furthermore,we evaluated the influence of groove depth on tissue adhesion and thermal injury and then verified the antiadhesion stability of the bionic electrode.This study demonstrates a promising approach for improving the cutting performance of surgical electrodes. | Kaikai LI Longsheng LU Huaping CHEN Guoxiang JIANG Huanwen DING Min YU Yingxi XIE | 2023 | Frontiers of Mechanical Engineering2023，18，1： | 0 |
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