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| 1 | 番茄植株与白粉病菌互作过程中叶片胞间蛋白变化的初步分析显示文摘利用SDS -PAGE方法分析番茄抗白粉病过程中番茄叶片胞间蛋白 (IntercellularProtein)的变化趋势 ,比较了含有Ol 1的抗病品种和感病品种间的差异 ,从分泌蛋白的角度研究了番茄抗白粉病的机理。 | 李成伟 王晓武 Guusje Bonnema | 2003 | 中国蔬菜2003，，3： | 5 |
| 2 | Genome-wide transcriptome analysis reveals molecular pathways involved in leafy head formation of Chinese cabbage(Brassica rapa)显示文摘Chinese cabbage plants go through seedling and rosette stages before forming their leafy head.Chinese cabbage plants resemble pak-choi plants at their seedling stage,but in their rosette stage the leaves of Chinese cabbage differentiate,as they increase in size with shorter petioles.In order to understand the molecular pathways that play a role in leafy head formation,transcript abundance of young emerging leaves was profiled during development of two Chinese cabbage genotypes and a single pak-choi genotype.The two Chinese cabbages differed in many aspects,among others earliness,leaf size and shape,leaf numbers,and leafy head shape.Genome-wide transcriptome analysis clearly separated the seedling stages of all three genotypes together with the later stages from pak-choi,from the later developmental stages of both Chinese cabbages(rosette,folding,and heading).Weighted correlation network analysis and hierarchical clustering using Euclidean distances resulted in gene clusters with transcript abundance patterns distinguishing the two Chinese cabbages from pak-choi.Three clusters included genes with transcript abundance affected by both genotype and developmental stage,whereas two clusters showed only genotype effects.This included a genotype by developmental stage cluster highly enriched with the MapMan category photosynthesis,with high expression during rosette and folding in Chinese cabbages and low expression in the heading inner leaves that are not exposed to light.The other clusters contained many genes in the MapMan categories Cell,showing again differences between pak-choi and both Chinese cabbages.We discuss how this relates to the differences in leaf blade growth between Chinese cabbage and pak-choi,especially at the rosette stage.Overall,comparison of the transcriptome between leaves of two very different Chinese cabbages with pak-choi during plant development allowed the identification of specific gene categories associated with leafy head formation. | XiaoXue Sun Ram Kumar Basnet Zhichun Yan Johan Bucher Chengcheng Cai Jianjun Zhao Guusje Bonnema | 2019 | Horticulture Research2019，6，1： | 4 |
| 3 | Genetic relationships within Brassica rapa as inferred from AFLP fingerprints显示文摘 | Jianjun Zhao Xiaowu Wang Bo Deng Ping Lou Jian Wu Rifei Sun Zeyong Xu Jaap Vromans Maarten Koornneef Guusje Bonnema | 2005 | Theoretical and Applied Genetics2005，，7： | 1 |
| 4 | What makes turnips:anatomy,physiology and transcriptome during early stages of its hypocotyl-tuber development显示文摘Brassica species are characterized by their tremendous intraspecific diversity,exemplified by leafy vegetables,oilseeds,and crops with enlarged inflorescences or above ground storage organs.In contrast to potato tubers that are edible storage organs storing energy as starch and are the vegetative propagation modules,the storage organs of turnips,grown from true seed,are swollen hypocotyls with varying degrees of root and stem that mainly store glucose and fructose.To highlight their anatomical origin,we use the term“hypocotyl-tuber”for these turnip vegetative storage organs.We combined cytological,physiological,genetic and transcriptomic approaches,aiming to identify the initial stages,molecular pathways and regulatory genes for hypocotyl-tuber induction in turnips(B.rapa subsp.rapa).We first studied the development of the hypocotyl zone of turnip and Pak choi and found that 16 days after sowing(DAS)morphological changes occurred in the xylem which indicated the early tuberization stage.Tissue culture experiments showed a clear effect of auxin on hypocotyl-tuber growth.Differentially expressed genes between 1 and 6 weeks after sowing in turnip hypocotyls,located in genomic regions involved in tuber initiation and/or tuber growth defined by QTL and selective sweeps for tuber formation,were identified as candidate genes that were studied in more detail for their role in hypocotyl-tuber formation.This included a Bra-FLOR1 paralogue with increased expression 16 DAS,when the hypocotyl starts swelling,suggesting dual roles for duplicated flowering time genes in flowering and hypocotyltuber induction.Bra-CYP735A2 was identified for its possible role in tuber growth via trans-zeatin.Weigthed Coexpression Network Analysis(WGCNA)identified 59 modules of co-expressed genes.Bra-FLOR1 and Bra-CYP735A2 were grouped in a module that included several genes involved in carbohydrate transport and metabolism,cell-wall growth,auxin regulation and secondary metabolism that serve as starting points to illuminate the transcriptional regulation of hypocotyl-tuber formation and development. | Mengyang Liu Niccolo Bassetti Stefan Petrasch Ningwen Zhang Johan Bucher Shuxing Shen Jianjun Zhao Guusje Bonnema | 2019 | Horticulture Research2019，6，1： | 1 |
| 5 | The Brassica rapa FLC homologue FLC2 is a key regulator of flowering time, identified through transcriptional co-expression networks显示文摘 | Dong Xiao Jian J. Zhao Xi L. Hou Ram K. Basnet Dunia P.D. Carpio Ning W. Zhang Johan Bucher Ke Lin Feng Cheng Xiao W. Wang Guusje Bonnema | 2013 | Journal of Experimental Botany2013，，14： | 1 |
| 6 | Genetic relationships within Brassica rapa as inferred from AFLP fingerprints显示文摘 | Jianjun Zhao Xiaowu Wang Bo Deng Ping Lou Jian Wu Rifei Sun Zeyong Xu Jaap Vromans Maarten Koornneef Guusje Bonnema | 2005 | Theoretical and Applied Genetics2005，，7： | 1 |
| 7 | Genetic relationships within Brassica rapa as inferred from AFLP fingerprints显示文摘 | Jianjun Zhao Xiaowu Wang Bo Deng Ping Lou Jian Wu Rifei Sun Zeyong Xu Jaap Vromans Maarten Koornneef Guusje Bonnema | 2005 | Theoretical and Applied Genetics2005，，7： | 1 |
| 8 | Genetic analysis of the “head top shape” quality trait of Chinese cabbage and its association with rosette leaf variation显示文摘The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the heading leaves curve inward and overlap at the top,is the shape preferred by consumers.To understand the genetic regulation of HTS,we generated a large segregating F 2 population from a cross between pak choi and Chinese cabbage,with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves.HTS was correlated with plant height,outer/rosette leaf length,and petiole length.A high-density genetic map was constructed.Quantitative trait locus(QTL)analysis resulted in the identification of 22 QTLs for leafy head-related traits,which included five HTS QTLs.Bulked segregant analysis(BSA)was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms.Interestingly,the HTS QTLs colocalized with QTLs for plant height,outer/rosette leaf,and petiole length,consistent with the observed phenotypic correlations.Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait. | Xiaoxue Sun Ying Gao Yin Lu Xiaomeng Zhang Shuangxia Luo Xing Li Mengyang Liu Daling Feng Aixia Gu Xueping Chen Shuxin Xuan Yanhua Wang Shuxing Shen Guusje Bonnema Jianjun Zhao | 2021 | Horticulture Research2021，8，1： | 0 |
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