| 1 | Research Progress on Agrobacterium tumefaciens-based Transgenic Technology in Brassica rapa显示文摘Brassica rapa L.is cultivated globally and consumed in many areas worldwide.Using the transgenic Agrobacterium-mediated transformation method,which is a reproducible and efficient technique,genes can be transferred into various B.rapa species.This review summarizes the processes involved in Agrobacterium-mediated transformation of B.rapa,including surface seed sterilization,co-cultivation with A.tumefaciens,induction of callus/shoot/root formation,and confirmation of transgenic plants.In addition,factors such as the Agrobacterium strain,plant genotype,explant age,transformation efficiency of the hybrid or inbred line,and the concentrations of N6-benzyl amino purine and naphthalene acetic acid,are discussed.And this review shows clearly how to do it,what to do,and what not to do in the transgenic Agrobacterium-mediated in Brassica rapa.The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenic B.rapa production,thereby benefiting both basic and applied research. | LI Guoliang YUE Lixin LI Fei ZHANG Shifan ZHANG Hui QIAN Wei FANG Zhiyuan WU Jian WANG Xiaowu ZHANG Shujiang SUN Rifei | 2018 | Horticultural Plant Journal2018，4，3： | 3 |
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