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| 1 | A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV) infected pneumonia(standard version)显示文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named '2019 novel coronavirus(2019-nCoV)' by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV. | Ying-Hui Jin Lin Cai Zhen-Shun Cheng Hong Cheng Tong Deng Yi-Pin Fan Cheng Fang Di Huang Lu-Qi Huang Qiao Huang Yong Han Bo Hu Fen Hu Bing-Hui Li Yi-Rong Li Ke Liang Li-Kai Lin Li-Sha Luo Jing Ma Lin-Lu Ma Zhi-Yong Peng Yun-Bao Pan Zhen-Yu Pan Xue-Qun Ren Hui-Min Sun Ying Wang Yun-Yun Wang Hong Weng Chao-Jie Wei Dong-Fang Wu Jian Xia Yong Xiong Hai-Bo Xu Xiao-Mei Yao Yu-Feng Yuan Tai-Sheng Ye Xiao-Chun Zhang Ying-Wen Zhang Yin-Gao Zhang Hua-Min Zhang Yan Zhao Ming-Juan Zhao Hao Zi Xian-Tao Zeng Yong-Yan Wang Xing-Huan Wang 无 | 2020 | Military Medical Research2020，7，1： | 161 |
| 2 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 3 | Application of bone transgenic zebrafish in anti-osteoporosis chemical screening显示文摘Background: The zebrafish(Danio rerio) has recently been shown to be an ideal model to study bone disease including osteoporosis. The zebrafish osteoporosis model could be induced by glucocorticoid treatment with chemical staining for reflecting the level of bone mineralization. However, this methodology was unstable.Here, we developed a novel methodology to directly evaluate the bone mass and density.Methods: We generated and used the bone of transgenic zebrafish Tg(ola.sp7:nlsGFP) to evaluate the bone mass and density by measuring the areal extent and the integrated optical density(IOD) of enhanced green fluorescent protein(eGFP).This methodology was further compared with the traditional chemically stained method showing the bone mineralization. Furthermore, genes related to zebrafish osteoporosis were analyzed by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results: Our results of new methods were consistent with those from chemically stained fish, following glucocorticoid-induction or epimedium flavonoid(FE)-rescue treatments. q RT-PCR analyses on mRNA levels revealed that glucocorticoid induces osteoporosis by downregulating the expression of osteoblast-related factors osterix,osteocalcin, and osteopontin, and upregulating the expression of osteoclast-related factor tartrate-resistant acid phosphatase. In FE-rescued fish, the expression of osteogenic factors osterix, osteocalcin, and osteopontin were increased.Conclusion: Compared to the traditional chemical staining methods, the new osteoporosis model using Tg(ola.sp7:nlsGFP) is more convenient and efficient for studying osteoporosis in vivo, and especially for high-throughput anti-osteoporosis drug screening. | Hong-xin Huang Hao Lin Fen Lan Yong-fu Wu Zhen-guo Yang Jing-jing Zhang | 2018 | Animal Models and Experimental Medicine2018，1，1： | 8 |
| 4 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 5 | Color-related chlorophyll and carotenoid concentrations of Chinese kale can be altered through CRISPR/Cas9 targeted editing of the carotenoid isomerase gene BoaCRTISO显示文摘The carotenoid isomerase gene(BoaCRTISO)of Chinese kale was targeted and edited using the CRISPR/Cas9 system in the present study.The results showed a high mutation rate(81.25%),and 13 crtiso mutants were obtained.Only two types of mutations,insertions and replacements,were found.Both the total and individual carotenoid and chlorophyll concentrations of the biallelic and homozygous mutants were reduced,and the total levels declined by 11.89–36.33%.The color of the biallelic and homozygous mutants changed from green to yellow,likely reflecting a reduction in the color-masking effect of chlorophyll on carotenoids.The expression levels of most carotenoid and chlorophyll biosynthesis-related genes,including CRTISO,were notably lower in the mutants than in the WT plants.In addition,the functional differences between members of this gene family were discussed.In summary,these findings indicate that CRISPR/Cas9 is a promising technique for the quality improvement of Chinese kale and other Brassica vegetables. | Bo Sun Min Jiang Hao Zheng Yue Jian Wen-Li Huang Qiao Yuan Ai-Hong Zheng Qing Chen Yun-Ting Zhang Yuan-Xiu Lin YanWang Xiao-Rong Wang Qiao-Mei Wang Fen Zhang Hao-Ru Tang | 2020 | Horticulture Research2020，7，1： | 7 |
| 6 | Micro RNA profiles and potential regulatory pattern during the early stage of spermatogenesis in mice显示文摘Spermatogenesis is a complicated and poorly understood process that relies on the precise regulation of the self-renewal and differentiation of spermatogonia. In many organisms, micro RNAs(mi RNAs) are involved in multiple developmental processes as critical regulators of transcriptional and post-transcriptional gene silencing. This study investigated the expression pattern of mi RNAs in type B spermatogonia cells(BSc) and primary spermatocytes(PSc) of mice, using a high-throughput small RNA sequencing system. The results revealed that the expression levels of Let-7 family mi RNAs were remarkably high in both cell types. Furthermore, the expression levels of mi R-21, mi R-140-3p, mi R-103, mi R-30 a, mi R-101 b and mi R-99 b were decreased during the transformation from BSc to PSc. These mi RNAs target vital genes that participate in apoptosis, cell proliferation and differentiation, junction assembly and cell cycle regulation. These results highlight the indispensable role of mi RNAs in spermatogenesis. | LUO MengMeng HAO LiLi HU Fen DONG YaNan GOU LiXia ZHANG WenDian WANG Xin ZHAO YuHui JIA MengChun HU SongNian ZHANG XiuJun | 2015 | Science China(Life Sciences)2015，58，5： | 6 |
| 7 | Variability of microphysical characteristics in the “21·7” Henan extremely heavy rainfall event显示文摘In this study, significant rainfall microphysical variability is revealed for the extremely heavy rainfall event over Henan Province in July 2021(the “21·7” Henan EHR event) using a dense network of disdrometers and two polarimetric radars.The broad distributions of specific drop size distribution(DSD) parameters are identified in heavy rainfall from the disdrometer observations, indicating obvious microphysical variability on the surface. A K-means clustering algorithm is adopted to objectively classify the disdrometer datasets into separate groups, and distinct DSD characteristics are found among these heavy rainfall groups. Combined with the supporting microphysical structures obtained through radar observations, comprehensive microphysical features of the DSD groups are derived. An extreme rainfall group is dominantly formed in the deep convection over the plain regions, where the high number of concentrations and large mean sizes of surface raindrops are underpinned by both active ice-phase processes and efficient warm-rain collision-coalescence processes in the vertical direction. Convection located near orographic regions is characterized by restricted ice-phase processes and high coalescence efficiency of liquid hydrometeors, causing the dominant DSD group to comprise negligible large raindrops. Multiple DSD groups can coexist within certain precipitation episodes at the disdrometer stations, indicating the potential microphysical variability during the passage of convective system on the plain regions. | Gang CHEN Kun ZHAO Yinghui LU Yuanyuan ZHENG Ming XUE Zhe-Min TAN Xin XU Hao HUANG Haonan CHEN Fen XU Ji YANG Shushi ZHANG Xueqi FAN | 2022 | Science China Earth Sciences2022，65，10： | 4 |
| 8 | Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells显示文摘INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agent independently and almost simultaneouslyand named hepatitis G virus and GB virus C,respectively[4-7]. | Hao Ren Fen Lu Zhu Shi Ying Zhu Yan Bin Song Zhong Tian Qi Department of Microbiology, Second Military Medical University, Shanghai 200433, China | 2001 | World Journal of Gastroenterology2001，7，1： | 3 |
| 9 | A molecular roadmap for induced multi-lineage transdifferentiation of fibroblasts by chemical combinations显示文摘 | Xiaoping Han Hao Yu Daosheng Huang Yang Xu Assieh Saadatpour Xia Li Lengmei Wang Jie Yu Luca Pinello Shujing Lai Mengmeng Jiang Xueying Tian Fen Zhang Yanhong Cen Yuko Fujiwara Wei Zhu Bin Zhou Tianhua Zhou Hongwei Ouyang Jianan Wang Guo-Cheng Yuan Shumin Duan Smart H Orkin Guoji Guo | 2017 | Cell Research2017，27，3： | 2 |
| 10 | Literature review and economic evaluation of oral and intramuscular ziprasidone treatment among patients with schizophrenia in China显示文摘Background Over 10 million Chinese are affected by schizophrenia. The annual cost of schizophrenia in China was estimated at US$2586 per patient.Aims The study has two aims:(1) to conduct a targeted literature review of the economic literature on oral ziprasidone in China, and(2) to develop an inpatient economic model that compared the cost of intramuscular ziprasidone with other regimens including electroconvulsive therapy(ECT) for the management of acute agitation in patients with schizophrenia from a hospital's perspective in China.Methods A targeted literature review was conducted using PubMed and the Chinese literature databases for studies published between January 2007 and December2017. Studies that assessed costs associated with oral ziprasidone treatment for schizophrenia in China were summarised. In the inpatient economic model,cost measures included hospital room and board,antipsychotics,ECT and medications for the management of extrapyramidal symptoms(EPS). Input for standard antipsychotic regimens and unit cost were obtained from the literature. Hospital length of stay(LOS), utilisation of ECT and incidence of EPS were derived from the literature and supplemented/validated with a survey of psychiatrists in China. Cost was presented in 2017 Chinese yuan.Results The average estimated LOS was 29 days with ziprasidone, 33 days with risperidone+benzodiazepine,32 days with olanzapine, 35 days with haloperidol and 29 days with ECT. The cost of antipsychotics was $1260 with ziprasidone, $137 with risperidone+benzodiazepine, $913 with olanzapine and $210 with haloperidol; ECT treatment costs $785. The base-case analysis suggested that higher antipsychotic cost with ziprasidone was offset by savings with shorter LOS. Using intramuscular ziprasidone for acute management was associated with a total cost of $11157, the lowest among all antipsychotic regimens($11 424 with risperidone+benzodiazepine, $11 711 with olanzapine and $11 912 with haloperidol) and slightly higher than ECT($10 606). The cost of antipsychotics and ECT accounted for 1 %-11 % of the total cost. Varying LOS between the lower and upper bounds of the 95% CI, the total cost was comparable between these regimens.Conclusions Overall, the cost for the management of acute agitation was similar between intramuscular ziprasidone and other antipsychotics. Compared with other antipsychotics, the higher medication cost of intramuscular ziprasidone can be offset by savings with shorter hospital stay. The results from this economic analysis were complementary to the findings in the published literature assessing the economic outcomes of oral ziprasidone. | Yu-Chen Yeh Xin Yu Congpei Zhang Wei Hao Fen Du Dongdong Liu Lili Yang Xin Gao | 2018 | General Psychiatry2018，31，6： | 2 |
| 11 | In situ electron paramagnetic resonance (EPR) study of surface oxygen species on Au/ZnO catalyst for low-temperature carbon monoxide oxidation显示文摘 | Hao Z Fen L Lu G Q et ol | 2001 | Appl Catal A2001，213，2： | 1 |
| 12 | The cyclic voltammetry study of Ag seed layer on p-silicon(100) substrate 显示文摘 | JING Fenning TONG Hao WANG Chunming | 2004 | J Solid State Electrochem2004，8，： | 1 |
| 13 | Epidemiological Survey of Hemoglobinopathies Based on Next-Generation Sequencing Platform in Hunan Province,China显示文摘Objective This study was aimed at investigating the carrier rate of,and molecular variation in,α-andβ-globin gene mutations in Hunan Province.Methods We recruited 25,946 individuals attending premarital screening from 42 districts and counties in all 14 cities of Hunan Province.Hematological screening was performed,and molecular parameters were assessed.Results The overall carrier rate of thalassemia was 7.1%,including 4.83%forα-thalassemia,2.15%forβ-thalassemia,and 0.12%for bothα-andβ-thalassemia.The highest carrier rate of thalassemia was in Yongzhou(14.57%).The most abundant genotype ofα-thalassemia andβ-thalassemia was-α^(3.7)/αα(50.23%)andβ^(IVS-Ⅱ-654)/β^(N)(28.23%),respectively.Fourα-globin mutations[CD108(ACC>AAC),CAP+29(G>C),Hb Agrinio and Hb Cervantes]and sixβ-globin mutations[CAP+8(C>T),IVS-Ⅱ-848(C>T),-56(G>C),beta nt-77(G>C),codon 20/21(-TGGA)and Hb Knossos]had not previously been identified in China.Furthermore,this study provides the first report of the carrier rates of abnormal hemoglobin variants andα-globin triplication in Hunan Province,which were 0.49%and 1.99%,respectively.Conclusion Our study demonstrates the high complexity and diversity of thalassemia gene mutations in the Hunan population.The results should facilitate genetic counselling and the prevention of severe thalassemia in this region. | XI Hui LIU Qin XIE Dong Hua ZHOU Xu TANG Wang Lan TANG De Guo ZENG Chun Yan WANG Qiong NIE Xing Hui PENG Jin Ping GAO Xiao Ya WU Hong Liang ZHANG Hao Qing QIU Li FENG Zong Hui WANG Shu Yuan ZHOU Shu Xiang HE Jun ZHOU Shi Hao ZHOU Fa Qun ZHENG Jun Qing WANG Shun Yao CHEN Shi Ping ZHENG Zhi Fen MA Xiao Yuan FANG Jun Qun LIANG Chang Biao WANG Hua | 2023 | Biomedical and Environmental Sciences2023，36，2： | 1 |
| 14 | The Energy Consumption and Environmental Impacts of Biomass Power Generation System in China显示文摘 | Song Jing Hui Huang Ze Hao Kan Wei Min Zhan Zhi Gang Liao Yan Fen Ma Xiao Qian | 2012 | 2012 (512)2012，，512： | 1 |
| 15 | Characteristics of Viral Shedding in Respiratory Samples and Specific Antibodies Production in 564 COVID-19 Patients显示文摘Positive nucleic acid(NA)results have been found in recovered and discharged COVID-19 patients,but the proportion is unclear.This study was designed to analyze the recurrent positive rate of NA results after consecutively negative results,and the relationship between the specific antibody production and positive NA rate.According to Strengthening the Reporting of Observational Studies in Epidemiology guidelines,data of inpatients in Sino-French New City Branch of Tongji Hospital between Jan.28 and Mar.6,2020 were collected.A total of 564 COVID-19 patients over 14 years old who received the examinations of NA and antibodies against SARS-CoV-2 were included.Days of viral shedding and specific antibodies were recorded and assessed.Among NA tests in respiratory samples(throat swabs,nasopharyngeal swabs,sputum and flushing fluid in alveoli),the patients with all-negative NA results accounted for 17.20%,those with single-positive results for 46.63%,and those with multiple-positive results for 36.17%respectively.Besides,the recurrent positive NA results after consecutively negative results appeared in 66 patients(11.70%).For multiple-positive patients,median viral shedding duration was 20 days(range:1 to 57 days).Of the 205 patients who received 2 or more antibody tests,141(68.78%)had decreased IgG and IgM concentrations.IgM decreased to normal range in 24 patients,with a median of 44 days from symptom onset.Viral shedding duration was not significantly correlated with gender,age,disease severity,changes in pulmonary imaging,and antibody concentration.It is concluded that antibody level and antibody change had no significant correlation with the positive rate of NA tests and the conversion rate after continuous negative NA tests.In order to reduce the recurrent positive proportion after discharge,3 or more consecutive negative NA test results with test interval more than 24 h every time are suggested for the discharge or release from quarantine. | Jing GONG Hui DONG Ding-kun WANG Fu-er LU Zhao-yi HUANG Ke FANG Wen-ya HUANG Fen YUAN Xing CHEN Qing-song XIA Le-yi MA Fan WU Hao SU Min-min GONG Yue-heng TANG Ke-xin NIE Zhi WANG Sheng-hao TU Ming-min ZHANG Jing-bin LI | 2021 | Current Medical Science2021，41，1： | 1 |
| 16 | Variable-rate con- trolled sprinklers for precision irrigation 显示文摘 | Han Wenting Fen Hao Wu Pute | 2007 | ASABE Annual In- ternational Meeting Technical Papers2007，，6： | 1 |
| 17 | Finite Element Simulation of Bending Fretting Fatigue Considering Ratchetting and Cyclic Hardening显示文摘 | Yi Long Bai Guang Wen Ma Sheng Wang Hao Meng Fen Xia Fu Jiu Ke | 2013 | Key Engineering Materials2013，，535： | 1 |
| 18 | In situ electron paramagnetic resonance (EPR) study of surface oxygen species on Au/ZnO catalyst for low-temperature carbon monoxide oxidation 显示文摘 | Hao Zh Fen L Lu G Q | 2001 | Applied Catalysis A General2001，213，： | 1 |
| 19 | The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples显示文摘 | XING‐BIN XIE SHEN LI RUI‐FEN ZHANG JING ZHAO YING‐CHUN CHEN QIANG ZHAO YU‐XIN YAO CHUN‐XIANG YOU XIAN‐SHENG ZHANG YU‐JIN HAO | 2012 | Plant Cell & Environment2012，，11： | 1 |
| 20 | BRAF mutation is a prognostic biomarker for colorectal liver metastasectomy显示文摘 | Hao‐Wei Teng Yu‐Chung Huang Jen‐Kou Lin Wei‐Shone Chen Tzu‐Chen Lin Jeng‐Kai Jiang Chueh‐Chuan Yen Anna Fen‐Yau Li Hsei‐Wei Wang Shih‐Ching Chang Yuan‐Tzu Lan Chun‐Chi Lin Huann‐Sheng Wang Shung‐Haur Yang | 2012 | J Surg Oncol2012，，2： | 1 |
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