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| 1 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 2 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 3 | Methylation Modifications in Eukaryotic Messenger RNA显示文摘RNA methylation modifications have been found for decades of years, which occur at different RNA types of numerous species, and their distribution is species-specific. However, people rarely know their biological functions. There are several identified methylation modifications in eukaryotic messenger RNA(mRNA), such as N7-methylguanosine(m7G) at the cap, N6-methyl-20-O-methyladenosine(m6Am), 20-O-methylation(Nm) within the cap and the internal positions, and internal N6-methyladenosine(m6A) and 5-methylcytosine(m5C). Among them, m7G cap was studied more clearly and found to have vital roles in several important mRNA processes like mRNA translation, stability and nuclear export. m6A as the most abundant modification in mRNA was found in the 1970s and has been proposed to function in mRNA splicing, translation, stability, transport and so on. m6A has been discovered as the first RNA reversible modification which is demethylated directly by human fat mass and obesity associated protein(FTO) and its homolog protein, alkylation repair homolog 5(ALKBH5). FTO has a special demethylation mechanism that demethylases m6A to A through two over-oxidative intermediate states: N6-hydroxymethyladenosine(hm6A) and N6-formyladenosine(f6A). The two newly discovered m6A demethylases, FTO and ALKBH5, significantly control energy homeostasis and spermatogenesis, respectively, indicating that the dynamic and reversible m6A,analogous to DNA and histone modifications, plays broad roles in biological kingdoms and brings us an emerging field 'RNA Epigenetics'. 5-methylcytosine(5mC) as an epigenetic mark in DNA has been studied widely, but m5C in mRNA is seldom explored. The bisulfide sequencing showed m5C is another abundant modification in mRNA, suggesting that it might be another RNA epigenetic mark.This review focuses on the main methylation modifications in mRNA to describe their formation, distribution, function and demethylation from the current knowledge and to provide future perspectives on functional studies. | Jun Liu Guifang Jia | 2014 | Journal of Genetics and Genomics2014，41，1： | 13 |
| 4 | Arab/c/opste N^(6)-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies显示文摘The biological functions of the epitranscriptomic modification N^(6)-methyladenosine(m^(6)A)in plants are not fully understood.CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m^(6)A-binding YTH domain.Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m^(6)A-binding function in alternative polyadenylation.Here,we charac-terized CPSF30-L as an Arabidopsis m^(6)A reader whose m^(6)A-binding function is required for the floral tran-sition and abscisic acid(ABA)response.We found that the m^(6)A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies,where CPSF30-L mainly recognizes m*A-modified far-upstream elements to control polyadenylation site choice.Deficiency of CPSF30-L lengthens the 3'untranslated region of three phenotypes-related transcripts,thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity.Collectively,this study uncovers a new molecular mechanism for m^(6)A-driven phase separation and polyadenylation in plants. | Peizhe Song Junbo Yang Chunling Wang Qiang Lu Linqing Shi Subiding Tayier Guifang Jia | 2021 | Molecular Plant2021，14，4： | 12 |
| 5 | Reversible RNA Modification N^1-methyladenosine(m^1A) in mRNA and tRNA显示文摘More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N^6-methyladenosine(m^6A), N^1-methyladenosine(m^1A) has been found as a reversible modification in tRNA and mRNA. m^1A occurs at positions 9, 14, and 58 of tRNA, with m^1A58 being critical for t RNA stability. Other than the hundreds of m^1A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m^1A also identifies>20 m^1A sites in mitochondrial genes. m^1A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of m^1A in mRNA and tRNA, covering high-throughput sequencing methods developed for m^1A methylome, m^1A-related enzymes(writers and erasers), as well as its functions in mRNA and tRNA. | Chi Zhang Guifang Jia | 2018 | Genomics, Proteomics & Bioinformatics2018，16，3： | 8 |
| 6 | Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acid显示文摘Kainic acid can simulate excitatory amino acids in vitro.Neural stem cells,isolated from newborn Wistar rats,were cultured in vitro and exposed to 100-4 000 μM kainic acid for 7 days to induce neuronal cell differentiation,causing the number of astrocytes to be significantly increased.Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone.Experimental findings suggest that gastrodin reduces the excitability of kainic acid and induces neural stem cell differentiation into neurons. | Guifang Sun Zhihao Yuan Boai Zhang Yanjie Jia Yangfei Ji Xingrong Ma Yu Liu Yanru Liu Quanqing Wen Yanling Zhao | 2012 | Neural Regeneration Research2012，7，12： | 4 |
| 7 | Optical fiber amplifiers for space-division multiplexing显示文摘最近,空间部门 multiplexing (SDM ) 技术使用多核心纤维(MCF ) 和很少模式纤维(FMF ) 被介绍了进光纤维通讯增加传播能力。光纤维放大器的二种主要类型基于 Erbiumdoped 纤维(EDF ) 和拉曼效果被开发了在 MCF 和 FMF 放大信号。在这份报纸,我们考察了这些放大器的原则和配置。 | Dagong JIA Haiwei ZHANG Zhe JI Neng BAI Guifang LI | 2012 | Frontiers of Optoelectronics2012，5，4： | 3 |
| 8 | 0.5981μm Sum Frequency Mixing in KTP Crystal显示文摘Based on accurate values of refractive indices,the phase matching angles(θ_(m))and effective nonlinear coeficients(d_(eff))for type I and I I sum frequency mixing of 1.0795 and 1.3414μm radiations were calculated in KTP crystal.The optimum phase matching conditions are thatθm=75.74o in the XZ plane(Ф=0)and deff=17.42 × 10^(-9) esu.The calculated results agree well with the experimental results.Thus,the cw 0.5981μm sum frequency radiation was achieved in KTP crystal using a 1.0795 and 1.3414μm double-wavelength Nd:YAP cw laser. | SHEN Hongyuan ZHOU Yuping LIN Wenxiong ZENG Ruirong YU Guifang HUANG Chenghui ZENG Zhengdong ZHANG Weijia JIA Shouquan JIANG Peizhi XIAO Chaoliang | 1991 | Chinese Physics Letters1991，8，4： | 2 |
| 9 | Neutron activation analysis and Mdssbauer spectroscopy research on coloring mechanism of ancient Chinese Guan porcelain显示文摘The content of 30 kinds of coloring elements in Guan porcelain in the Southern Song Dynasty has been determined by neutron activation analysis (NAA). Iron is the major coloring element. Mossbauer spectroscopy has shown that the iron of the glaze exists in the form of structural iron (Fe2+, Fe3+). The quantitative relationship between the main wavelength of every color glaze and the relative content (Fe2+/Fe3+) of structural iron has been determined. Thus the coloring mechanism of Guan porcelain in the Southern Song Dynasty has been revealed entirely. | ZHAO Weijuan, LI Guoxia, GAO Zhengyao, ZHANG bin, LI Rongwu, WU Xiaoli, YAO Guifang, JIA Xiuqin, HAN Song & HUANG Zhongxiang1. Department of Physics, Zhengzhou University, Zhengzhou 450052, China 2. Department of Architecture, Zhengzhou Technology University, Zhengzhou 450002, China 3. Department of Physics, Beijing Normal University, Beijing 100875, China 4. Hangzhou Museum of Guan kiln, Hangzhou 310008, China 5. Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China | 2000 | Chinese Science Bulletin2000，45，20： | 2 |
| 10 | The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis显示文摘The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress responses.The RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in Arabidopsis.However,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant mRNA.We demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(ABA)and that ALKBH9 B has m^(6)A demethylation activity on endogenous mRNA.Knocking out ALKBH9 B led to hypersensitivity to ABA treatment during seed germination and early seedling development.We further showed that ALKBH9 B removes the m^(6)A modification in the ABA INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following ABA treatment,affecting the stability of these mRNAs.Furthermore,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in ABA responses depended on ABI3 and ABI5.Our findings reveal the important roles of the m^(6)A modification in ABA responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating ABA responses post-transcriptionally. | Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia | 2022 | Journal of Integrative Plant Biology2022，64，12： | 1 |
| 11 | Investigating the Role of Raindrop Impact on Hydrodynamic Mechanism of Soil Erosion Under Simulated Rainfall Conditions显示文摘 | Juan An Fenli Zheng Jia Lu Guifang Li | 2012 | Soil Science2012，，8： | 1 |
| 12 | Xuefu Zhuyu Capsule in the treatment of cardiovascular and cerebrovascu- lar disease mechanism显示文摘 | Jia Jiehong Tang Guifang Jia Zhongbao | 2010 | Beijing doctor of traditional Chi- nese medicine2010，，19： | 1 |
| 13 | Xue fu Zhu yu Capsulein the treatment of cardiovascular and cerebrovaseular disease meehanism显示文摘 | Jia Jie hong Tang Guifang Jia Zhongbao | 2010 | Beijing doctor of traditional Chinese medicine2010，19，： | 1 |
| 14 | Author Correction: 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘In the initial published version of this article, there was a mistake in the P value for the correlation between gene-expression changes and 5 hmC changes in tumors. The correct P value should be same as the P value shown in Fig. S6A: 9.8?×?10?6 (mistakenly shown as “9.8?×?106” in the main text). This correction does not affect the description of results or the conclusions of this study, since the range of P value is between 0 and 1. | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2019 | Cell Research2019，29，7： | 1 |
| 15 | Stereoselective degradation of fungicide benalaxyl in soils and cucumber plants显示文摘 | Wang Xinquan Jia Guifang Qiu Jing | 2006 | Chirality2006，19，4： | 1 |
| 16 | Response of Chinese fir seedlings to low phosphorus stress and analysis of gene expression differences显示文摘Chinese fir(Cunninghamia lanceolata) is an excellent fast-growing timber species occurring in southern China and has significant value in the forestry industry. In order to enhance the phosphorus utilization efficiency in Chinese fir, four clones named X6, S3, S39 and FK were used, and low phosphorus(LP) stress experiments were performed to analyze the response of different clones to phosphorus deficiency. According to the results on seedling height, maximum root length, leaf blade aspect ratio, root ratio, malondialdehyde content, acid phosphates activity,proline content, soluble protein level, and chlorophyll a and b levels of the tested clones, compared to the control groups(CK), the phosphorus high efficiency clone X6 was screen out for transcriptome sequencing experiments. De novo RNA-seq was then used to sequence the root transcriptomes of X6 under LP stress and CK, and we then compared the gene expression differences under the two conditions. A total of 3416 SDEGs were obtained by comparing the LP and CK groups, among which 1742 were up-regulated and 1682 were down-regulated. All SDEGs obtained from the LP and CK treated samples were subjected to KEGG annotation and classification. Through classification statistical analysis using WEGO software,607 SDEGs obtained KEGG pathway annotations, which were related to 206 metabolic pathways. In Chinese fir subjected to LP stress, 53 SDEGs related with phosphorus metabolism, and phosphate uptake and transport were obtained from our transcriptome data. Based on the phosphorus metabolism pathway obtained by KEGG classification, combined with previously report on gene annotation related with phosphorus metabolism, the enzymes encoded by SDEG related with phosphorus metabolism and their expression pattern were mapped onto phosphorus metabolism pathway. | Jianhui Li Dingwei Luo Guifang Ma Licui Jia Jinliang Xu Huahong Huang Zaikang Tong Yong-Quan Lu | 2019 | Journal of Forestry Research2019，30，1： | 1 |
| 17 | Assessing environmental impact:Micro-energy network optimization in a Chinese industrial park显示文摘Micro-energy systems contribute significantly to environmental improvement by reducing dependence on power grids through the utilization of multiple renewable energy sources.This study quantified the environmental impact of a micro-energy network system in an industrial park through a life cycle assessment using the operation of the micro-energy network over a year as the functional unit and“cradle-to-gate”as the system boundary.Based on the baseline scenario,a natural gas generator set was added to replace central heating,and the light pipes were expanded to constitute the optimized scenario.The results showed that the key impact categories for both scenarios were global warming,fine particulate matter formation,human carcinogenic toxicity,and human non-carcinogenic toxicity.The overall environmental impact of the optimized scenario was reduced by 68%compared to the baseline scenario.A sensitivity analysis of the key factors showed that electricity from the power grid was the key impact factor in both scenarios,followed by central heating and natural gas.Therefore,to reduce the environmental impact of network systems,it is necessary to further optimize the grid power structure.The research approach can be used to optimize micro-energy networks and evaluate the environmental impact of different energy systems. | Guanzhun Cao Chuan Feng Tong Li Hongjuan Zhang Xiaoyao Guo Wen Li Yanshuang Jia Leping Chen Yuan Xu Qingsong Wang Guifang Chen Xueliang Yuan | 2024 | Chinese Journal of Population,Resources and Environment2024，22，1： | 0 |
| 18 | Diploid chromosome-level reference genome and population genomic analyses provide insights into Gypenoside biosynthesis and demographic evolution of Gynostemma pentaphyllum (Cucurbitaceae)显示文摘Gynostemma pentaphyllum(Thunb.)Makino is a perennial creeping herbaceous plant in the family Cucurbitaceae,which has great medicinal value and commercial potential,but urgent conservation efforts are needed due to the gradual decreases and fragmented distribution of its wild populations.Here,we report the high-quality diploid chromosome-level genome of G.pentaphyllum obtained using a combination of next-generation sequencing short reads,Nanopore long reads,and Hi-C sequencing technologies.The genome is anchored to 11 pseudo-chromosomes with a total size of 608.95 Mb and 26588 predicted genes.Comparative genomic analyses indicate that G.pentaphyllum is estimated to have diverged from Momordica charantia 60.7 million years ago,with no recent whole-genome duplication event.Genomic population analyses based on genotyping-by-sequencing and ecological niche analyses indicated low genetic diversity but a strong population structure within the species,which could classify 32 G.pentaphyllum populations into three geographical groups shaped jointly by geographic and climate factors.Furthermore,comparative transcriptome analyses showed that the genes encoding enzyme involved in gypenoside biosynthesis had higher expression levels in the leaves and tendrils.Overall,the findings obtained in this study provide an effective molecular basis for further studies of demographic genetics,ecological adaption,and systematic evolution in Cucurbitaceae species,as well as contributing to molecular breeding,and the biosynthesis and biotransformation of gypenoside. | Xiao Zhang Yuhe Zhao Yixuan Kou Xiaodan Chen Jia Yang Hao Zhang Zhe Zhao Yuemei Zhao Guifang Zhao Zhonghu Li | 2023 | Horticulture Research2023，10，1： | 0 |
| 19 | New Edges of RNA Adenosine Methylation Modifications显示文摘Recently an article published in Molecular Cell reveals the mechanism of a nuclear N6-methyladenosine(m^6A)reader,the YTH domain-containing protein 1(YTHDC1),in regulating pre-m RNA splicing[1].Meanwhile,two additional articles published in Nature and Nature Chemical Biology report | Ye Wang Guifang Jia | 2016 | Genomics, Proteomics & Bioinformatics2016，14，3： | 0 |
| 20 | Protective effects of gastrodine on dendritic spines in kainic acid-injured neurons显示文摘EphB2 affects neuronal cells by altering the dendritic spines. The present study analyzed the neu-roprotective effects of gastrodine by measuring EphB2 expression in rat neural cells cultured in vitro and injured by kainic acid. Gastrodine (12.5, 25, and 50 mg/L) improved morphology in kainic acid-injured neurons, reduced lactate dehydrogenase leakage, decreased neuronal apoptosis, and increased EphB2 expression in neuronal cells. A moderate dose of 25 mg/L gastrodine resulted in the most significant effects. These results suggested that gastrodine suppressed the neurotoxic effects of excitatory amino acids and provided neuroprotection by remodeling neuronal dendritic spines. | Yangfei Ji Boai Zhang Yanjie Jia Guifang Sun Yu Liu Tao Peng Yanru Liu Xingrong Ma | 2011 | Neural Regeneration Research2011，6，36： | 0 |
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