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| 1 | A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice显示文摘A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice (Oryza sativa L.) varieties.In this study,we generated an F 2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64),an elite indica small-grain cultivar.Using QTL analysis,17 QTLs for five grain traits were detected on four different chromosomes.Eight of the QTLs were newly-identified in this study.In particular,qGL3-1,a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation,was fine-mapped to the 17 kb region of chromosome 3.A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL.A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10,resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid.This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat.Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect,and is suitable for use in molecular marker-assisted selection. | Zejun Hu Haohua He Shiyong Zhang Fan Sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang Xiaojin Luo | 2012 | Journal of Integrative Plant Biology2012，54，12： | 41 |
| 2 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 3 | Genetic Analysis and Fine Mapping of a Novel Semidominant Dwarfing Gene LB4D in Rice显示文摘一个矮子变异的、指明的 LB4D,在回交的子孙之中被获得到一根野米饭基因渗入线。LB4D 的基因分析显示矮子显型被使基因相形见绌的单个 semidominant 控制,它被称为 LB4D。异种根据 internode 减小的模式作为 dn 类型矮子异种被分类。另外,赤霉素(GA ) 反应测试证明 LB4D 植物对 GA 既不缺乏也不感觉迟钝。这研究发现由 LB4D 植物的那 tiller 形成被 40% 与野类型相比减少,与被识别了的另外的主导的矮子异种相对照,显示不同相形见绌机制可能涉及 LB4D 主导的异种。在 F1 植物的植物高度的减小在不同基因背景从 27.9% ~ 38.1%,证明 LB4D 施加了更强壮的主导的相形见绌效果。用从在异质接合的 LB4D 和装饰用的梨树栽培变种 Nipponbare 之间的一个十字导出的大 F2 和 F3 人口, LB4D 基因在染色体 11 的短手臂上对在标记 Indel 4 和 Indel G 之间的 46 kb 区域局部性,并且四预言的基因在目标区域作为候选人被识别。 | Fei Liang Xiaoyun Xin Zejun Hu Jiandi Xu Gang Wei Xiaoyin Qian Jinshui Yang Haohua He Xiaojin Luo | 2011 | Journal of Integrative Plant Biology2011，53，4： | 6 |
| 4 | Target-triggered inhibiting oxidase-mimicking activity of platinum nanoparticles for ultrasensitive colorimetric detection of silver ion显示文摘The development of efficient methods for the detection of hazardous and toxic elements is extremely important for environmental security and public health. In this work, we developed a facile colorimetric assaying system for Ag+ detection in aqueous solution. Chitosan-stabilized platinum nanoparticles(ChPtNPs) were synthesized and severed as an artificial oxidase to catalyze the oxidation of the substrate3,30,5,50-tetramethylbenzidine(TMB) and generate color signal. In the presence of Ag+, due to the strong metallophilic interactions between Ag+ and Pt2+ on the surface of Ch-PtNPs, Ag+ can weaken the affinity to the substrates and inactivate the catalytic activity of Ch-PtNPs, leading to decreased absorbance signal to varying degrees depending on Ag+ amount. Combing the specific binding between Ch-PtNPs and Ag+ with signal amplification procedure based on the Ch-PtNPs-catalyzed TMB oxidation, a sensitive,selective, simple, cost-effective, and rapid detection method for Ag+ can be realized. Ag+ ions in tap and lake waters have been successfully detected. We ensured that the proposed method can be a potential alternative for Ag+ determination in environmental samples. | Haohua Deng Shaobin He Xiuling Lin Lu Yang Zhen Lin Ruiting Chen Huaping Peng Wei Chen | 2019 | Chinese Chemical Letters2019，30，9： | 3 |
| 5 | Rice Heavy Metal P-type ATPase OsHMA6 Is Likely a Copper Efflux Protein显示文摘P1B-type heavy metal ATPases(HMAs)are transmembrane metal-transporting proteins that play a key role in metal homeostasis.We here reported the characterization of rice OsHMA6,a member of the P1B-type ATPase family.Phylogenetic tree analysis showed that OsHMA6 belonged to the Cu/Ag subgroup of the HMA family and had a close evolutionary relationship with OsHMA9.Amino acid sequence alignment showed 82.78%consistency between OsHMA6 and OsHMA9.OsHMA6 expressed in all organs at different growth stages,including spikelet,and abundant in leaf blades,however,OsHMA9 most strongly expressed in roots,but very low in spikelet.Excessive Cu^2+can up-regulate the expression of OsHMA6 and OsHMA9 in rice seedlings.The heterologous expression in yeast showed that OsHMA6 can significantly rescue the growth of yeast strain CM52 when supplied with 3 or 6 mmol/L Cu^2+.Compared with the empty vector pYES2,the Cu concentration in OsHMA6-pYES2 decreased by 23.4%and 30.3%under 3 or 6 mmol/L Cu2+,respectively.Subcellular localization revealed that OsHMA6 was located in the plasma membrane.These results suggested that OsHMA6,similar to OsHMA9,is likely a copper efflux protein located in the plasma membrane. | ZOU Wenli LI Chang ZHU Yajun CHEN Jingguang HE Haohua YE Guoyou | 2020 | Rice science2020，27，2： | 2 |
| 6 | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding显示文摘Semi-dwarfing improves the lodging resistance and yield of rice,and the vast majority of modern rice varieties harbor the sd1 allele to decrease plant height,resulting in reduced genetic diversity and negative agronomic traits.Thus,exploring alternative sources of dwarfism is imperative for rice breeding.Here,we identified a novel RGA1 allele,d1-w,from a local indica variety Xiaolixiang(XLX)using a map-based cloning approach.Compared with other rice varieties,RGA1 in XLX contained a unique single nucleotide polymorphism that resulted in an additional transcript and reduced functional RGA1 transcript level.The RGA1 from Nipponbare was introduced into XLX to estimate the value of d1-w in rice breeding.Compared with transgenic XLX plants(XLX^(D1)),XLX exhibited reduced plant height,increased stem strength,lower reactive oxygen species accumulation,delayed senescence,stronger photosynthesis,higher grain yield and quality(including external,milling and nutritional qualities),and enhanced resistance to drought and Rhizoctonia solani.Therefore,we proposed that the d1-w allele has potential as an excellent dwarfism resource for rice breeding. | LIU Yantong LI Ting JIANG Zhishu ZENG Chuihai HE Rong QIU Jiao LIN Xiaoli PENG Limei SONG Yongping ZHOU Dahu CAI Yicong ZHU Changlan FU Junru HE Haohua XU Jie | 2022 | Rice science2022，29，6： | 2 |
| 7 | Properties of the pigment in black rice显示文摘Pigment was extracted from pericarp of black rice variety Zixiangnuo. The effects of several factors, including pH, light, chemical reagents and foodstuff additives on the color, solubility and heat stability of the pigment were observed. The color of the pigment varied with different acid conditions. When the pH was below 3, a stronger absorption peak at 495nm was observed. If the pH was between 3 and 10, there was a platform in 400-495nm. No obvious absorption peak in visible light region was found when pH was above 10. As the pH increased, the color of the pigment changed, from deep red to light red until to yellowishbrown, e.g. pH 0-2, deep red; pH3-4, red pH5-6, quite red; pH9-10, faint red; pH11, faint brown yellow; pH12, light brown yellow; pH13, yellowish brown; pH14, deep yellowish brown. The change of color was reversible. The pigment was quite stable under nature light conditions. At pH 1, it was basically unchanged within 10 d under sunlight or within 60 d under indoor nature light. However, | HE Haohua,PAN Xiaoyun,RAO Zhixiang,and LIU Yibao,Dept of Agro,Jiangxi Agri Univ,Nanchang 330045,China | 1996 | Chinese Rice Research Newsletter1996，4，2： | 1 |
| 8 | Electrochemical polymerization of 3,4-ethylenedioxythiophene in aqueous micellar solution containing biocompatible amino acid-based surfactant显示文摘 | Wen Yangping Xu Jingkun He Haohua | 2009 | J Electroanal Chem2009，634，： | 1 |
| 9 | SUPEROXIDE DISMUTASE FAMILY GENES IN WATERMELON AND THEIR RESPONSES TO DIFFERENT ABIOTIC STRESSES显示文摘Superoxide dismutase(SOD)is an important enzyme in the antioxidant system of plants and plays a vital role in stress responses by maintaining the dynamic balance of reactive oxygen species(ROS)concentrations.Genome-wide analysis of the SOD gene family in various plant species has been conducted but little is known about this gene family in watermelon(Citrullus lanatus).Here,eight SOD genes were identified in the watermelon genome and are designated Cl CSD1-5,Cl FSD1-2 and Cl MSD according to their metal cofactors.Phylogenetic analysis shows that SOD proteins from various plant species can be classified into five groups and members in the same group possess the same metal cofactor and similar subcellular localizations.Expression analysis of the Cl SOD genes indicates that they had tissue-specific expression patterns with high expression in different tissues including the leaves,flowers and fruits.In addition,the expression of Cl SOD genes differed appreciably under salinity,drought and abscisic acid(ABA)treatments,indicating that they may be involved in ROS scavenging under different abiotic stresses via an ABA-dependent signaling pathway.These results lay the foundation for elucidating the function of Cl SOD genes in stress tolerance and fruit development in watermelon. | Yong ZHOU Linjuan OUYANG Dahu ZHOU Yicong CAI Haohua HE | 2021 | Frontiers of Agricultural Science and Engineering2021，8，4： | 1 |
| 10 | OsHG3 Affects Rice Palea Development, Grain Yield and Quality显示文摘Floral development is one of the most important determining factors of grain yield in cereal crops(Immink et al,2010).The rice inflorescence is composed of the spikelet,a specific structural unit in poaceae,which is greatly different from typical eudicots.However,several genes encoding the homologous of the proteins in ABCDE model were also found in rice genome(Luo and Zhu,2002).Lemma and palea are grass-specific floret tissues which interlock with each other to protect the inner floral organs and seeds from attack by pathogens,insects or abiotic stresses.Meanwhile,they have photosynthetic ability and provide amino acids and carbohydrates to grouting seeds. | Ouyang Linjuan Xu Minyu Hu Jiang Zhu Lixin Li Ting Xu Yilei Zhu Changlan Peng Xiaosong Chen Xiaorong He Haohua Xu Jie | 2020 | Rice science2020，27，5： | 0 |
| 11 | Natural variation of an autophagy-family gene among rice subspecies affects grain size and weight显示文摘Elucidating the genetic basis of natural variation in grain size and weight among rice varieties can help breeders develop high-yielding varieties.We identified a novel gene,GW3a(Grain Weight 3a)(LOC_Os03g27350),that affects rice grain size and weight.gw3a mutants showed higher total starch content and dry matter accumulation than the wild type(WT),Nipponbare,suggesting that GW3a negatively regulates grain size and weight.Moreover,our study found that GW3a interacted with OsATG8 by cleaving it,suggesting that GW3a may be involved in the assembly of autophagosomes and starch degradation in plants.The haplotype analysis of GW3a showed functional differences between indica and japonica rice.Taken together,we conclude that GW3a is expressed in the autophagosome pathway regulating starch metabolism in rice,affecting yield-related traits,such as grain size,grain weight and thousand grain weight(TGW).Our findings also shed new light on autophagy-mediated yield trait regulation,proposing a possible strategy for the genetic improvement of high-yield germplasm in rice. | Shiying Huang Peng Wang Caijing Li Qingwen Zhou Tao Huang Yicong Cai Qin Cheng Hui Wang Qi Zhong Zhihao Chen Liping Chen Haohua He Jianmin Bian | 2024 | The Crop Journal2024，12，1： | 0 |
| 12 | Intron Retention Fine-Tunes the Resistance of the Rice Mutant pls4 to Rice Sheath Blight(Rhizotonia solani AG I.1a)显示文摘OsPLS4 encodes aβ-ketoacyl carrier protein reductase(KAR).The role of OsPLS4 in rice sheath blight(Rhizoctonia solani)remains unclear.Our preliminary studies showed that premature leaf senescence mutants(pls4)were highly susceptive to sheath blight in the early stage of rice development.To explore the role of this gene in the development of rice sheath blight,the transcriptome profiles of the rice pls4 mutant and wild type were compared by RNA-seq.The results revealed 2,569 differentially expressed genes(DEGs).The down-regulated genes were significantly enriched in the defense response-related biological processes.These down-regulated genes included the chitinase genes and WRKY genes,which were significantly changed in pls4 mutants.Furthermore,467 genes induced significant alternative splicing(AS)events.Among them,intron retention(IR)affected gene expression levels and functions of the vitamin B6(VB6)metabolism pathway related to sheath blight.This result suggests that IR plays an important role in the sheath blight resistance of mutant pls4.Together,these results indicate that pls4 could be involved in the biological process of sheath blight via DEGs and the fine-tuning of IR.The present study provides a molecular basis for further investigation of the resistance of rice to sheath blight. | Shaochun Liu Jiamin Hu Haohua He Junru Fu Xu Jie Dahu Zhou Haihui Fu | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 0 |
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