|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Vaccination against Schistosoma japonicum Infection by DNA Vaccine Encoding Sj22.7 Antigen显示文摘在鼠标的效果, recombinant 原生质标志 pcDNA3-Sj22.7 与调停 liposome 的方法习惯于 transfectHeLa 房间, Sj22.7 mRNA 和蛋白质的表达式用反向的抄写聚合酶链反应,钠 dodecylsulfate-polyacrylamide 凝胶电泳和蛋白质印迹被检验。然后, pcDNA3-Sj22.7 的能力在鼠标的种痘以后根据蠕虫减小率和鸡蛋减小率被分析免于 Schistosomajaponicum 挑战感染。特定的 IgG 抗体和 T 淋巴细胞增长反应的浆液层次也是坚定的。在挑战感染以后,驾驶 Sj22.7 的干扰素(IFN )-gamma andinterleukin (IL )-4 也被确定。结果证明 pcDNA3-Sj22.7 能表示 Sj22.7 mRNAand 在 vitro.Immunization 的蛋白质导致了29.70%的蠕虫减小率;鸡蛋减小率of47.25%(肝)并且51.73%(肠),并且25.90%的鸡蛋减小率(鸡蛋每女性),在 连接group.Enzyme 的免疫吸着剂试金和蛋白质印迹分析显示了的 pcDNA3-Sj22.7 建议重要反肥沃的正式就职那使对从与 pcDNA3-Sj22.7 使免疫的鼠标的 Sj22.7.Tlymphocytes 的产生特定的 IgG 显示出的鼠标免疫怒气房间的一种重要增长反应 torSj22.7.The 文化证明 IFN-gamma 的那分泌物增加了但是 IL-4decreased.The 结果显示由 pcDNA3-Sj22.7 的 DNA 种痘对足够对 S.japonicum 感染得到保护的免疫的重要层次。DNA 疫苗能导致重要细胞、体液的有免疫力的反应,并且显示占优势的 T 助手房间类型 1 类型免疫者回答,它在鼠标对挑战感染贡献保护的免疫。 | Gan DAI Shiping WANG Junlong YU Shaorui XU Xianchu PENG Zhuo HE Xueqin LIU Songhua ZHOU and Fen LIU Institute of Schistosomiasis Research.Xiangya School of Medicine,Central South University,Changsha 410078 China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，1： | 5 |
| 2 | Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity. | YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China | 2007 | Science China(Life Sciences)2007，50，2： | 4 |
| 3 | Marsdenia tenacissima injection induces the apoptosis of prostate cancer by regulating the AKT/GSK3β/STAT3 signaling axis显示文摘Marsdenia tenacissima injection,a standard Marsdenia tenacissima extract(MTE),has been approved as an adjuvant therapeutic agent for various cancers.Our previous study showed that MTE inhibited the proliferation and metastasis of prostate cancer(PCa)cells.However,the underlying mechanisms and active ingredients of MTE against PCa were not completely understood.This study revealed that MTE induced significant decreases in cell viability and clonal growth in PCa cells.In addition,MTE induced the apoptosis of DU145 cells by reducing the mitochondrial membrane potential and increasing the expression of Cleaved Caspase 3/7,Cyt c,and Bax.In vivo,DU145 xenografted NOD-SCID mice treated with MTE showed significantly decreased tumor size.TUNEL staining and Western blot confirmed the pro-apoptotic effects of MTE.Network pharmacology analysis collected 196 ingredients of MTE linked to 655 potential targets,and 709 PCa-associated targets were retrieved,from which 149 overlapped targets were screened out.Pathway enrichment analysis showed that the HIF-1,PI3K-AKT,and ErbB signaling pathways were closely related to tumor apoptosis.Western blot results confirmed that MTE increased the expression of p-AKT^(Ser473) and p-GSK3β^(Ser9),and decreased the expression of p-STAT3^(Tyr705) in vitro and in vivo.A total of 13 compounds in MTE were identified by HPLC-CAD-QTOF-MS/MS and UPLCQTOF-MS/MS.Molecular docking analysis indicated that six compounds may interact with AKT,GSK3β,and STAT3.In conclusion,MTE induces the endogenous mitochondrial apoptosis of PCa by regulating the AKT/GSK3β/STAT3 signaling axis,resulting in inhibition of PCa growth in vitro and in vivo. | LI Xiaolan HE Songhua LIANG Wei ZHANG Weiquan CHEN Xin LI Qiaofeng YANG Xin LIU Yanying ZHU Dan LI Li LIU Buming SU Zhiheng CHEN Jie GUO Hongwei | 2023 | Chinese Journal of Natural Medicines2023，21，2： | 2 |
| 4 | Intercalation and hybrid heterostructure integration of two-dimensional atomic crystals with functional organic semiconductor molecules显示文摘Van der Waals(vdW)integration affords semiconductor heterostructures without constrains of lattice matching and opens up a new realm of functional devices by design.A particularly interesting approach is the electrochemical intercalation of two-dimensional(2D)atomic crystal and formation of superlattices,which can provide scalable production of novel vdW heterostructures.However,this approach has been limited to the use of organic cations with non-functional aliphatic chains,therefore failed to take the advantage of the vast potentials in molecular functionalities(electronic,photonic,magnetic,etc.).Here we report the integration of 2D crystal(MoS_(2),WS_(2),highly oriented pyrolytic graphite(HOPG),WSe_(2) as model systems)with electrochemically inert organic molecules that possess semiconducting characteristics(including perylene-3,4,9,10-tetracarboxylic dianhydride(PTCDA),pentacene and fullerene),through on-chip electrochemical intercalation.An unprecedented long-range spatial feature of intercalation has been achieved,which allowed facile assembly of a vertical MoS_(2)-PTCDA-Si junction.The intercalated heterostructure shows significant modulation of the lateral transport,and leads to a molecular tunneling characteristic at the vertical direction.The general intercalation of charge neutral and functional molecules defines a versatile platform of inorganic/organic hybrid vdW heterostructures with significantly extended molecular functional building blocks,holding great promise in future design of nano/quantum devices. | Wen He Han Zang Songhua Cai Zhangyan Mu Cheng Liu Mengning Ding Peng Wang Xinran Wang | 2020 | Nano Research2020，13，11： | 2 |
| 5 | Synthesis and properties of CrN x /amorphous-WC nanocomposites prepared using hybrid arc ion plating and direct current magnetron sputtering显示文摘 | Shihong Zhang Mingxi Li Yizhu He Tongyul Cho Huigon Chun Jaehong Yoon Songhua Si Huisheng Li | 2010 | Thin Solid Films2010，，2： | 1 |
| 6 | Analysis of damage efficiency of HPMW on typical active radar guided missile显示文摘 | GUO Hanzhong HE Songhua OU Jianping | 2013 | Applied Mechanics and Materials2013，26，： | 1 |
| 7 | Determination of Chlorogenic Acid in Stems of Mussaenda pubescens Ait.f.by High Performance Liquid Chromatography显示文摘[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens. | Qianhua ZHANG Dongping TU Lichun ZHAO Yi LUO Songhua HE Tao ZHU | 2018 | Agricultural Biotechnology2018，7，6： | 1 |
| 8 | Development and characterization of polymorphic microsatellite markers in Linum usitatissimum显示文摘 | Xin Deng Songhua Long Dongfeng He Xiang Li Yufu Wang Jia Liu Xinbo Chen | 2010 | Journal of Plant Research2010，，1： | 1 |
| 9 | Flexible piezoelectric PVDF nanofiber film sensor by blow spinning显示文摘Flexible sensors can be widely applied in the area of lightweight,wearable and intelligent electronic devices.In aerospace,for example,flexible sensors are suitable for measuring pressure in complex aircraft structures.In recent years,flexible sensors have been well developed;however,most of them lack circuit parts,meaning that the sensors have to be connected to an external electrical source.Additionally,the current traditional design limits their flexibility.Thus,this article proposes a self-powered flexible sensor based on PVDF nanofiber film,which is created through an efficient blow spinning method.The piezoelectric PVDF nanofiber films were prepared,and the flexible pressure sensor was assembled.The PVDF nanofiber film sensor showed good sensitivity and stability,and it can be applied for the detection of human physiological signals. | HE Wei DAI ZhiJun ZOU KangKang LI XueYing HAO SongHua WANG HaoLun | 2023 | Science China(Technological Sciences)2023，66，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员