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| 1 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 2 | Gene diagnosis and targeted breeding for blast-resistant Kongyu 131without changing regional adaptability显示文摘The fungus Magnaporthe oryzae threatens the rice production of Kongyu 131 (KY131),a leading japonica variety in Northeast China.In this study,two rice lines,KP1 and KP2-Hd1,were obtained by introgressing the blast resistance genes Pi1 and Pi2 into KY131,respectively.However,both lines headed later than KY131.RICE60K SNP array analysis showed that Hd1 closely linked to Pi2 was introgressed into KP2-Hd1,and the linkage drag of Hd1 was broken by recombination.On the other hand,no known flowering genes were introgressed into KP1.Gene diagnosis by resequencing six flowering genes showed that KP1 carried functional Hd16 and Ghd8 alleles.Due to its suppression role in heading under long-day conditions,Ghd8 was chosen as the target for gene editing to disrupt its function.Four sgRNAs targeting different sites within Ghd8 were utilized to induce large-deletion mutations,which were easy to detect via agarose gel electrophoresis.All the ghd8-mutated KP1 lines were resistant to rice blast disease and headed earlier than the control KP1,even than KY131,under natural long-day conditions,which ensures its growth in Northeast China.This study confirmed that a combination of gene diagnosis and targeted gene editing is a highly efficient way to quickly eliminate undesired traits in a breeding line. | Xiangchun Zhou Gonghao Jiang Longwei Yang Lei Qiu Ping He Chunxiao Nong Yunyue Wang Yuqing He Yongzhong Xing | 2018 | Journal of Genetics and Genomics2018，45，10： | 7 |
| 3 | Global analysis of CCT family knockout mutants identifies four genes involved in regulating heading date in rice显示文摘Many genes encoding CCT domain-containing proteins regulate flowering time. In rice(Oryza sativa), 41 such genes have been identified, but only a few have been shown to regulate heading date. Here, to test whether and how additional CCT family genes regulate heading date in rice, we classified these genes into five groups based on their diurnal expression patterns. The expression patterns of genes in the same subfamily or in close phylogenetic clades tended to be similar. We generated knockout mutants of the entire gene family via CRISPR/Cas9. The heading dates of knockout mutants of only 4 of 14 genes previously shown to regulate heading date were altered, pointing to functional redundancy of CCT family genes in regulating this trait. Analysis of mutants of four other genes showed that OsCCT22, OsCCT38, and OsCCT41 suppress heading under long-day conditions and promote heading under short-day conditions. OsCCT03 promotes heading under both conditions and upregulates the expression of Hd1 and Ehd1, a phenomenon not previously reported for other such genes. To date, at least 18 CCT domaincontaining genes involved in regulating heading have been identified, providing diverse, flexible gene combinations for generating rice varieties with a given heading date. | Jia Zhang Xiaowei Fan Yong Hu Xiangchun Zhou Qin He Liwen Liang Yongzhong Xing | 2021 | Journal of Integrative Plant Biology2021，63，5： | 6 |
| 4 | Biodegradation of polycyclic aromatic hydrocarbons in sediments from the Daliao River watershed, China显示文摘The Daliao River, as an important water system in Northeast China, was reported to be heavily polluted by polycyclic aromatic hydrocarbons (PAHs). Aerobic biodegradations of four selected PAHs (naphthalene, phenanthrene, uorene and anthracene) alone or in their mixture in river sediments from the Daliao River water systems were studied in microcosm systems. Eects of additional carbon source, inorganic nitrogen and phosphorus, temperature variation on PAHs degradation were also investigated. Results showed that the degradation of phenanthrene in water alone system was faster than that in water-sediment combined system. Degradation of phenanthrene in sediment was enhanced by adding yeast extract and ammonium, but retarded by adding sodium acetate and not significantly inuenced by adding phosphate. Although PAHs could also be biodegraded in sediment under low temperature (5°C), much lower degradation rate was observed. Sediments from the three main streams of the Daliao River water system (the Hun River, the Taizi River and the Daliao River) demonstrated dierent degradation capacities and patterns to four PAHs. Average removal rates (15 or 19 d) of naphthalene, phenanthrene, uorene and anthracene by sediment were in the range of 0.062-0.087, 0.005-0.066, 0.008- 0.016 and 0-0.059 mg/(L·d), respectively. As a result, naphthalene was most easily degraded compound, anthracene was the hardest one. In multiple PAHs systems, the interactions between PAHs inuenced each PAH biodegradation. | QUAN Xiangchun, TANG Qian, HE Mengchang, YANG Zhifeng, LIN Chunye, GUO Wei State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Beijing Normal University, Beijing 100875, China. | 2009 | Journal of Environmental Sciences2009，21，7： | 2 |
| 5 | Heavy metal contamination in the sediment of the Second Songhua River显示文摘 | Chunye LIN Mengchang HE Baixing YAN Xiangchun QUAN Yuxiang ZHOU Wei GUO Zhifeng YANG | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 1 |
| 6 | Distribution of polycyclic aromatic hydrocarbons in water, suspended particulate matter and sediment from Daliao River watershed, China显示文摘 | Wei Guo Mengchang He Zhifeng Yang Chunye Lin Xiangchun Quan Haozheng Wang | 2006 | Chemosphere2006，，1： | 1 |
| 7 | Enhancement of organic pollutant biodegradation by unltrasound irradiation in a biological activated carbon membrane reactor 显示文摘 | Liu Hong He Yunhua Quan Xiangchun | 2005 | Process Biochem2005，40，9： | 1 |
| 8 | Aliphatic and polycyclic aromatic hydrocarbons in the Xihe River, an urban river in China’s Shenyang City: Distribution and risk assessment显示文摘 | Wei Guo Mengchang He Zhifeng Yang Chunye Lin Xiangchun Quan | 2010 | Journal of Hazardous Materials2010，，2： | 1 |
| 9 | Effects of the 6VS.6AL translocation on agronomic traits and dough properties of wheat显示文摘 | Guiping Li Peidu Chen Shouzhong Zhang Xiue Wang Zhonghu He Yan Zhang He Zhao Huiyao Huang Xiangchun Zhou | 2007 | Euphytica2007，，3： | 1 |
| 10 | Multi-affect(ed):improving recommendation with similarity-enhanced user reliability and influence propagation显示文摘Traditional recommendation algorithms predict the latent interest of an active user by collecting rating information from other similar users or items.Recently,more and more recommendation systems attempt to involve social relations to improve recommendation performance.However,the existing works either leave out the user reliability or cannot capture the correlation between two users who are similar but not socially connected.Besides,they also take the trust value between users either 0 or 1,thus degenerating the prediction accuracy.In this paper,we propose an efficient social affect model,multi-affect(ed),for recommendation via incorporating both users'reliability and influence propagation.Specifically,the model contains two main components,i.e.,computation of user reliability and influence propagation,designing of user-shared feature space.Firstly,a reliability calculation strategy based on user similarity is developed for measuring the recommendation accuracy between users.Then,the factor of influence propagation relationship among users is taken into consideration.Finally,the multi-affect(ed)model is developed with user-shared feature space to generate the predicted ratings.Experimental results demonstrate that the proposed model achieves better accuracy than other counterparts recommendation techniques. | Lele HUANG Huifang MA Xiangchun HE Liang CHANG | 2021 | Frontiers of Computer Science2021，15，5： | 0 |
| 11 | Building and Application of the“Relay”Volunteer Teaching Model for Pre-service Teachers under“Internet Plus”显示文摘The educational practice of pre-service teachers is an essential part of teacher training.As an important form of educational practice,volunteer teaching can effectively improve the quality of teacher training and bridge the serving of basic education.Upon the literature review on existing volunteer teaching models,this paper develops a teaching community of teachers under“Internet Plus”and the prototype of a“relay”volunteer teaching model towards the integrated design of training undergraduate pre-teachers and improving the educational quality of small rural schools.The sound“relay”volunteer teaching model has been formed upon revisions around checking its completeness,optimizing its operability,and enhancing its stability using the design-based research paradigm according to the research path of“planning-application-reflection-improvement.”The practice has indicated positive effects of the new model in promoting the academic development of students in small rural schools,enhancing the classroom teaching competency of rural teachers,and improving the professionalism of pre-service teachers.The quality and efficiency of the new model can be improved through initiatives such as providing timely and efficient technical support,establishing a favorable and harmonious relationship between teachers and students,adopting guidance with double-qualified instructors for instant companionship,and implementing flexible incentives and restraints. | SHEN Junru GUO Shaoqing HE Xiangchun WANG Dong WANG Jiayang | 2022 | Frontiers of Education in China2022，17，3： | 0 |
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