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| 1 | Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions显示文摘在 Arabidopsis, nodulin 26-like 内在的蛋白质(捏) aquaporin 蛋白质的亚科由九个成员,组成哪个中的五个(NIP1; 1, NIP1; 2, NIP5; 1, NIP6; 1,并且 NIP7; 1 ) 以前被识别对亚砷酸盐可渗透。然而,角色在植物在亚砷酸盐的 root-to-shoot translocation 捏仍然保持糟糕理解。在这研究,用反向的基因策略, Arabidopsis NIP3; 1 被识别在亚砷酸盐压力条件下面在砷举起和 root-to-shoot 分发起一个重要作用。nip3; 1 loss-of-function 异种在亚砷酸盐忍耐显示了明显的改进为未葬生长和积累的更少砷在野类型的植物比那些射击,而 nip3; 1 nip1; 1 两倍异种显示出强壮的亚砷酸盐忍耐并且在亚砷酸盐压力条件下面改进了根和射击的生长。一个倡导者 -- glucuronidase 分析揭示了那 NIP3; 1 几乎专门在根被表示(与根尖端的异常) ,并且在酵母 Saccharomyces cerevisiae 的 heterologous 表示表明了那 NIP3; 1 能调停亚砷酸盐运输。一起拿,我们的结果建议那 NIP3; 1 在 Arabidopsis 涉及亚砷酸盐举起和 root-to-shoot translocation,可能作为被动、双向的亚砷酸盐 transporter。 | WenzhongXu Wentao Dai Huili Yan Sheng Li Hongling Shen Yanshan Chen Hua Xu Yangyang Sun Zhenyan He Mi Ma | 2015 | Molecular Plant2015，8，5： | 13 |
| 2 | Evidence of vacuolar compartmentalization of arsenic in the hyperaccumulator Pteris vittata显示文摘Pteris vittata can hyperaccumulate arsenic (As) to >1% of its dry weight without showing any signs of toxicity, indicating the existence of effective plant-internal detoxification mechanisms. Although vacuolar compartmentalization is known to play an important role in heavy metal detoxification and tolerance, direct evidence of arsenic compartmentalization in this hyperaccumulator was lacking. Here we report the subcellular localization of As in the callus of P. vittata. The callus induced from gametophytes of P. vittata exposed to 0.2 mmol/L arsenate for 20 days were examined by directly isolating cell walls, protoplasts and vacuoles, and determining arsenic concentrations. Of the total As in the callus, about 94% was in the protoplast, and of that, 91% was present in the vacuoles, indicating that vacuoles are a major storage site for As in P. vittata. In addition, the changes in the chemical components of vacuoles were analyzed by Fourier transform infrared spectroscopy (FTIR). | YANG XueXi CHEN Hui DAI XiaoJing XU WenZhong HE ZhenYan MA Mi | 2009 | Chinese Science Bulletin2009，54，22： | 8 |
| 3 | Effects of soaking process on arsenic and other mineral elements in brown rice显示文摘One of the main food crops in the world,rice can accumulate high levels of arsenic from flooded paddy soils,which seriously threatens human health.Soaking,a common processing method for brown rice products,especially for brown rice noodles,was investigated in this study.Japonica rice(Dao Hua Xiang No.2)and Indica rice(Ye Xiang You No.3)were selected for studying the effects of soaking on arsenic concentrations,species,and distributions.Results revealed that soaking can efficiently remove arsenic in these two rice varieties,and the main part of removal is endosperm with the maximal rate of about 40%.Inorganic arsenic(I-As)(about 85%)is the main species of arsenic reduction.Meanwhile,the variations of four other elements(i.e.,Mg,Ca,Zn,and Fe)were analyzed.Collectively,the findings of this study indicate that soaking can efficiently remove arsenic in brown rice under controlled soaking conditions,which thereby reduces the arsenic intake for brown rice customers. | Fan Zhang Fengying Gu Huili Yan Zhenyan He Bolun Wang Hao Liu Tingting Yang Feng Wang | 2020 | Food Science and Human Wellness2020，9，2： | 5 |
| 4 | FUNCTIONAL CHARACTERIZATION OF CADMIUM-RESPONSIVE GARLIC GENE ASMT2B: A NEW MEMBER OF METALLOTHIONEIN FAMILY显示文摘A new gene of metallothionein (MT) family was cloned from garlic (Allium sativum) seed-lings using RACE method and designated AsMT2b. The full length of AsMT2b cDNA was 520 bp encod-ing 80 amino acids. The deduced amino acids of AsMT2b showed that AsMT2b contained the char-acteristic structure of type 2 MT proteins, but the number and arrangement of the cysteine residues in the N- and C-terminal domains was different from other type 2 MT proteins. Semi-quantitative reverse transcriptase-PCR showed that transcript levels of AsMT2b were enhanced only in response to higher concentrations or longer incubation time of Cd. Such an expression pattern of AsMT2b greatly differed from that of other type 2 MT genes. Yeast cells transformed with this gene had improved resistance to Cd. AsMT2b overexpressing Arabidopsis showed stronger Cd tolerance and higher Cd accumulation compared with wild-type plants. These results sug-gest that AsMT2b should be useful in phytoremedia-tion of Cd-polluted soil in the future. | ZHANG Haiyan XUWenzhong DAI Wentao HE Zhenyan MA Mi | 2006 | Chinese Science Bulletin2006，51，4： | 5 |
| 5 | Dietary Leucine Requirement for Juvenile Large Yellow Croaker Pseudosciaena crocea (Richardson,1846)显示文摘Dietary leucine requirement for juvenile large yellow croaker, Pseudosciaena crocea Richardson 1846 (initial body weight 6.0 g±0.1 g) was determined using dose-response method.Six isonitogenous (crude protein 43%) and isoenergetic (19 kJ g-1) practical diets containing six levels of leucine (Diets 1-6) ranging from 1.23% to 4.80% (dry matter) were made at about 0.7% increment of leucine.Equal amino acid nitrogen was maintained by replacing leucine with glutamic acid.Triplicate groups of 60 individuals were fed to apparent satiation by hand twice daily (05:00 and 17:30).The water temperature was 26-32℃, salinity 26-30 and dissolved oxygen approximately 7 mg L-1 during the experimental period.Final weight (FW) of large yellow croaker initially increased with increasing level of dietary leucine but then decreased at further higher level of leucine.The highest FW was obtained in fish fed diet with 3.30% Leucine (Diet 4).FW of fish fed the diet with 4.80% Leucine (Diet 6) was significantly lower than those fed Diet 4.However, no significant differences were observed between the other dietary treatments.Feed efficiency (FE) and whole body composition were independent of dietary leucine contents (P>0.05).The results indicated that leucine was essential for growth of juvenile large yellow croaker.On the basis of FW, the optimum dietary leucine requirement for juvenile large yellow croaker was estimated to be 2.92% of dry matter (6.79% of dietary protein). | LI Yan AI Qinghui MAI Kangsen XU Wei CHENG Zhenyan HE Zhigang | 2010 | Journal of Ocean University of China2010，9，4： | 4 |
| 6 | Cadmium accumulation and oxidative burst in garlic ( Allium sativum )显示文摘 | Haiyan Zhang Yingnan Jiang Zhenyan He Mi Ma | 2005 | Journal of Plant Physiology2005，，9： | 1 |
| 7 | Hyperaccumulation of arsenic by callus, sporophytes and gametophytes of Pteris vittata cultured in vitro显示文摘 | Xuexi Yang Hui Chen Wenzhong Xu Zhenyan He Mi Ma | 2007 | Plant Cell Reports2007，，10： | 1 |
| 8 | Cadmium accumulation and oxidative burst in garlic(Allium sativum)显示文摘 | Zhang Haiyan Jiang Yingnan He Zhenyan | 2005 | Journal of plant physiology2005，162，： | 1 |
| 9 | Activating Connexin43 gap junctions primes adipose tissue for therapeutic intervention显示文摘Adipose tissue is a promising target for treating obesity and metabolic diseases.However,pharmacological agents usually fail to effectively engage adipocytes due to their extraordinarily large size and insufficient vascularization,especially in obese subjects.We have previously shown that during cold exposure,connexin43(Cx43)gap junctions are induced and activated to connect neighboring adipocytes to share limited sympathetic neuronal input amongst multiple cells.We reason the same mechanism may be leveraged to improve the efficacy of various pharmacological agents that target adipose tissue.Using an adipose tissue-specific Cx43 overexpression mouse model,we demonstrate effectiveness in connecting adipocytes to augment metabolic efficacy of theβ_(3)-adrenergic receptor agonist Mirabegron and FGF21.Additionally,combing those molecules with the Cx43 gap junction channel activator danegaptide shows a similar enhanced efficacy.In light of these findings,we propose a model in which connecting adipocytes via Cx43 gap junction channels primes adipose tissue to pharmacological agents designed to engage it.Thus,Cx43 gap junction activators hold great potential for combination with additional agents targeting adipose tissue. | Yi Zhu Na Li Mingyang Huang Xi Chen Yu AAn Jianping Li Shangang Zhao Jan-Bernd Funck Jianhong Cao Zhenyan He Qingzhang Zhu Zhuzhen Zhang Zhao VWang Lin Xu Kevin W.Williams Chien Li Kevin Grove Philipp E.Scherer | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 0 |
| 10 | Bacterial biogeography in China and its association to land use and soil organic carbon显示文摘●6102 high-quality sequencing results of soil bacterial samples were re-analyzed.●The type of land use was the principal driver of bacterial richness and diversity.●SOC content is positively correlated with key bacteria and total nitrogen content.Soil organic carbon(SOC)is the largest pool of carbon in terrestrial ecosystems and plays a crucial role in regulating atmospheric CO_(2) concentrations.Identifying the essential relationship between soil bacterial communities and SOC concentration is complicated because of many factors,one of which is geography.We systematically re-analyzed 6102 high-quality bacterial samples in China to delineate the bacterial biogeographic distribution of bacterial communities and identify key species associated with SOC concentration at the continental scale.The type of land use was the principal driver of bacterial richness and diversity,and we used machine learning to calculate its influence on microbial composition and their co-occurrence relationship with SOC concentration.Cultivated land was much more complex than forest,grassland,wetland and wasteland,with high SOC concentrations tending to enrich bacteria such as Rubrobacter,Terrimonas and Sphingomona.SOC concentration was positively correlated with the amounts of soil total nitrogen and key bacteria Xanthobacteraceae,Streptomyces and Acidobacteria but was negatively correlated with soil pH,total phosphorus and Micrococcaceae.Our study combined the SOC pool with bacteria and indicated that specific bacteria may be key factors affecting SOC concentration,forcing us to think about microbial communities associated with climate change in a new way. | Tao Lu Nuohan Xu Chaotang Lei Qi Zhang Zhenyan Zhang Liwei Sun Feng He Ning-Yi Zhou Josep Peñuelas Yong-Guan Zhu Haifeng Qian | 2023 | Soil Ecology Letters2023，5，4： | 0 |
| 11 | A novel nest-based scheduling method for mobile wireless body area networks显示文摘Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers from interference,which causes serious problems,such as wasting energy and degrading throughput.In reality,not all of the sensors in WBAN need to be active at the same time.Therefore,they can be divided into different groups so that each group works in turn to avoid interference.In this paper,a Nest-Based WBAN Scheduling(NBWS)algorithm is proposed to cluster sensors of the same types in a single or multiple WBANs into different groups to avoid interference.Particularly,we borrow the graph coloring theory to schedule all groups to work using a Time Division for Multimodal Sensor(TDMS)group scheduling model.Both theoretical analysis and experimental results demonstrate that the proposed NBWS algorithm performs better in terms of frequency of collisions,transmission delay,system throughput,and energy consumption compared to the counterpart methods. | Zhijun Xie Guangyan Huang Roozbeh Zarei Zhenyan Ji Hongwu Ye Jing He | 2020 | Digital Communications and Networks2020，6，4： | 0 |
| 12 | Polydiacetylene-based poly-ion complex enabling aggregation-induced emission and photodynamic therapy dual turn-on for on-demand pathogenic bacteria elimination显示文摘Poly-ion complex(PIC)integrating non-antibiotic theranostics holds great promise in the combat against drug-resistant bacteria.Photosensitizers with aggregation-induced emission(AIE)characteristic are particularly intriguing theranostic agents,but incorporating them into antibacterial PIC to enable both fluorescence and reactive oxygen species(ROS)generation turn-on is deemed a great challenge.Here we report the development of a PIC that can dually boost the fluorescence and ROS generation in the presence of pathogen bacteria.The PIC is constructed based on an anionic polydiacetylene poly(deca-4,6-diynedioic acid)(PDDA),which completely degrades in the presence of ROS.A cationic polymer quaternized poly(2-(dimethylamino)ethyl methacrylate)(PQDMA)that can disrupt bacterial membrane is co-loaded together with a highly efficient AIE photosensitizer TPCI in the PIC.PIC is nonfluorescent initially in that PDDA can quench the AIE of TPCI in PIC.When pathogenic bacteria are present,they can disturb the assembly of PIC to release TPCI,whose fluorescence turns on sensitively to indicate the existence of bacteria.The on-demand irradiation can be subsequently applied to excite TPCI,which generates ROS to degrade PDDA and deform the PIC.As a result,TPCI and PQDMA are completely released to eliminate bacteria through a synergy of turned-on photodynamic therapy(PDT)and membrane disruption.The highly efficient detection and inhibition against both Gramnegative and Gram-positive bacteria have validated this polydiacetylene-based PIC system as an effective non-antibiotic antibacterial theranostic platform as well as a new strategy to enable“turn-on”fluorescence sensing and imaging of AIE fluorophores. | Sidan Tian Yuan Lu Zhenyan He Qiang Yue Zhiyong Zhuang Yingzhou Wang Fanling Meng Liang Luo | 2022 | Science China Chemistry2022，65，9： | 0 |
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