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| 1 | Dietary nano-selenium alleviated intestinal damage of juvenile grass carp(Ctenopharyngodon idella)induced by high-fat diet:Insight from intestinal morphology,tight junction,inflammation,anti-oxidization and intestinal microbiota显示文摘In recent years,high-fat diet(HFD)has been widely applied in aquaculture,which reduces the intestinal health of cultured fish.The current study evaluated the protective effects of nano-selenium(nano-Se)on intestinal health of juvenile grass carp(Ctenopharyngodon idella)fed with HFD.A total of 135 experimental fish were fed with a regular diet(Con),a HFD(HFD)and a HFD containing nano-Se at 0.6 mg/kg(HSe)for 10 weeks.The results showed that dietary nano-Se significantly improved the survival rate and feed efficiency which were reduced by HFD in juvenile grass carp(P<0.05).Also,nano-Se(0.6 mg/kg)supplement alleviated intestinal damage caused by the HFD,thus maintaining the integrity of the intestine.Moreover,it significantly up-regulated the expression of genes related to tight junction(ZO-1,claudin-3 and occludin),anti-oxidization(GPx4a and GPx4b),and the protein of ZO-1 in the intestine of juvenile grass carp,which were depressed by the HFD(P<0.05).Furthermore,nano-Se supplementation significantly suppressed the expressions of genes related to the inflammation,including inflammatory cytokines(IL-8,IL-1β,IFN-γ,TNF-αand IL-6),signaling molecules(TLR4,p38 MAPK and NF-kB p65),and protein expression of NF-kB p65 and TNF-αin the intestine of juvenile grass carp which were induced by the HFD(P<0.05).Besides,dietary nano-Se normalized the intestinal microbiota imbalance of juvenile grass carp caused by the HFD through increasing the abundance of the beneficial bacteria,e.g.,Fusobacteria.Finally,dietary nano-Se increased the production of short chain fatty acids(SCFA)in the intestine,especially for butyric acid and caproic acid,which were negatively related to the increase of intestinal permeability and inflammation.In summary,supply of nano-Se(0.6 mg/kg)in HFD could effectively alleviate intestinal injury of juvenile grass carp by improving intestinal barrier function and reducing intestinal inflammation and oxidative stress.These positive effects may be due to the regulation of nano-Se on intestinal microbiota and the subsequently increased beneficial SCFA levels. | Sha Liu Haibo Yu Pengju Li Chi Wang Guohao Liu Xiaotian Zhang Cheng Zhang Meng Qi Hong Ji | 2022 | Animal Nutrition2022，，1： | 4 |
| 2 | Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration显示文摘胰腺的癌症是有高死亡率的恶意的癌症。在胰腺的癌症房间的糜蛋白酶 C 的数量仅仅是在正常房间发现了那中的 20% 个。糜蛋白酶 C 被报导了涉及癌症房间 apoptosis,但是它胰腺的癌症房间移植上的效果是不清楚的。我们执行了房间迁居擦伤试金和 Transwell 实验,并且发现那个房间迁居能力是在胰腺的癌症 Aspc-1 房间的 downregulated 那 overexpressed 糜蛋白酶 C,而房间迁居能力是在糜蛋白酶 C 在被压制的 Aspc-1 房间的 upregulated。在胶化电气泳动 / 团 spectrometry 方法微分的二维的荧光被用来识别是在是有 plasmids 的 transfected 导致任何一个 overexpression 或压制了糜蛋白酶 C 的表示的 Aspc-1 房间表示的差别的蛋白质。在 26 识别微分蛋白质之中, 18 最显然是的 cytokeratin 与糜蛋白酶 C 表示相关。Cytokeratin 18 在癌症的早阶段在发展纸巾被表示,并且高度在大多数癌被表示。我们推测了那糜蛋白酶 C 可能在与 cytokeratin 的关系调整胰腺的癌症房间移植 18 表示。 | Haibo Wang Wei Sha Zhixue Liu Cheng-Wu Chi | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 2 |
| 3 | Construction of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue显示文摘Vascularization and bone regeneration are two closely related processes during bone reconstruction.A three-dimensional(3D)scaffold with porous architecture provides a suitable microenvironment for vascular growth and bone formation.Here,we present a simple and general strategy to construct a nanofibrous poly(L-lactide)/poly(ε-caprolactone)(PLLA/PCL)scaffold with interconnected perfusable microchannel networks(IPMs)based on 3D printing technology by combining the phase separation and sacrificial template methods.The regular and customizable microchannel patterns within the scaffolds(spacings:0.4 mm,0.5 mm,and 0.6 mm;diameters:0.8 mm,1 mm,and 1.2 mm)were made to investigate the effect of microchannel structure on angiogenesis and osteogenesis.The results of subcutaneous embedding experiment showed that 0.5/0.8-IPMs(spacing/diameter=0.5/0.8)and 0.5/1-IPMs(spacing/diameter=0.5/1)scaffolds exhibited more vascular network formation as compared with other counterparts.After loading with vascular endothelial growth factor(VEGF),VEGF@IPMs-0.5/0.8 scaffold prompted better human umbilical vein endothelial cells(HUVECs)migration and neo-blood vessel formation,as determined by Transwell migration,scratch wound healing,and chorioallantoic membrane(CAM)assays.Furthermore,the microangiography and rat cranial bone defects experiments demonstrated that VEGF@IPMs-0.5/0.8 scaffold exhibited better performance in vascular network formation and new bone formation compared to VEGF@IPMs-0.5/1 scaffold.In summary,our results suggested that the microchannel structure within the scaffolds could be tailored by an adjustable caramel-based template strategy,and the combination of interconnected perfusion microchannel networks and angiogenic factors could significantly enhance vascularization and bone regeneration. | Jiani Gu Qianqian Zhang Mengru Geng Weizhong Wang Jin Yang Atta ur Rehman Khan Haibo Du Zhou Sha Xiaojun Zhou Chuanglong He | 2021 | Bioactive Materials2021，6，10： | 1 |
| 4 | Phosphatase of Regenerating Liver-3 Promotes Motility and Metastasis of Mouse Melanoma Cells显示文摘 | Xiaopeng Wu Hu Zeng Xianming Zhang Ying Zhao Haibo Sha Xiaomei Ge Minyue Zhang Xiang Gao Qiang Xu | 2004 | The American Journal of Pathology2004，，6： | 1 |
| 5 | Disruption of a novel regu- latory locus results in decreased Bdnf expression, obesity, and type 2 diabetes in mice显示文摘 | Haibo Sha Jingyue Xu Jing Tang | 2007 | Physiol Genomics2007，31，： | 1 |
| 6 | Safety and feasibility of laparoscopic surgery for colorectal and gastric cancer under the Chinese multi-site practice policy:admittance standards of competence are needed显示文摘Background:The multi-site practice(MSP)policy has been practiced in China over 10 years.This study aimed to investigate the safety and feasibility of performing laparoscopic surgery for colorectal cancer(LSCRC)and gastric cancer(LSGC)under the Chinese MSP policy.Methods:We collected and analysed the data from 1,081 patients who underwent LSCRC or LSGC performed by one gastrointestinal surgeon in his original hospital(n=573)and his MSP institutions(n=508)between January 2017 and December 2020.Baseline demographics,intraoperative outcomes,post-operative recovery,and pathological results were compared between the original hospital and MSP institutions,as well as between MSP institutions with and without specific competence(surgical skill,operative instrument,perioperative multi-discipline team).Results:In our study,690 patients underwent LSCRC and 391 patients underwent LSGC.The prevalence of post-operative complications was comparable for LSCRC(11.5%vs 11.1%,P=0.89)or LSGC(15.2%vs 12.6%,P=0.46)between the original hospital and MSP institutions.However,patients in MSP institutions without qualified surgical assistant(s)and adequate instruments experienced longer operative time and greater intraoperative blood loss.The proportion of patients with inadequate lymph-node yield was significantly higher in MSP institutions than in the original hospital for both LSCRC(11.5%vs 21.2%,P<0.01)and LSGC(9.8%vs 20.5%,P<0.01).Conclusion:For an experienced gastrointestinal surgeon,performing LSCRC and LSGC outside his original hospital under the MSP policy is safe and feasible,but relies on the precondition that the MSP institutions are equipped with qualified surgical skills,adequate operative instruments,and complete perioperative management. | Zhenghao Cai Haiqin Song Zhenfeng Huang Abraham Fingerhut Ximo Xu Hao Zhong Zhigang Li Yingjie Zhang Dachong Sha Dandan Bao Haibo Wang Binghua Cai Shangbo Hua Yanhui Zhang Jianguang Sun Ke Ye Jianwen Li Yong Lu Bo Feng | 2022 | Gastroenterology Report2022，10，1： | 0 |
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