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| 1 | Small RNA Profiling Reveals Phosphorus Deficiency as a Contributing Factor in Symptom Expression for Citrus Huanglongbing Disease显示文摘Huanglongbing (HLB ) 是与类 Candidatus Liberibacter 的细菌被联系的破坏柠檬疾病(Ca。L.) 。强大的诊断工具和管理策略被需要控制 HLB。小 RNA (sRNA ) 玩的主人在调整主人回答到病原体感染的一个重要角色并且为许多人的疾病被用作早诊断的标记,包括癌症。决定是否柠檬 sRNAs 调整主人回答到 HLB, sRNAs 从柠檬 sinensis 被介绍有 Ca 的 10 和 14 星期柱子 grafting。L。asiaticus (Las ) 积极或健康的织物。十新 microRNAs (miRNAs ) , 76 保存 miRNAs,和许多小介入 RNA (siRNAs ) 被发现。几 miRNAs 和 siRNAs 被 Las 感染高度导致,并且能潜在地被开发进 HLB 的早诊断标记。miR399,被磷饥饿在另外的植物种类导致,被与类似于 HLB 的症状引起柠檬 stubborna 疾病的 Las 然而并非 Spiroplasma citri 的感染明确地导致。我们与健康的树相比在 Las 积极的柠檬树上发现了磷的 35% 减小。HLB 积极的香甜的橘子树的适用的磷 oxyanion 答案减少了 HLB 症状严厉并且显著地在西南佛罗里达在 3 年的地试用期间改进了水果生产。我们的分子,生理,并且地数据建议那磷缺乏被连接到 HLB 疾病 symptomology。 | Hongwei Zhao Ruobai Sun Ute Albrecht Chellappan Padmanabhan Airong Wanga Michael D. Coffey Thomas Girke Zonghua Wang Timothy J. Close Mikeal Roose Raymond K. Yokomi Svetlana Folimonova Georgios Vidalakis Robert Rouse Kim D. Bowman Hailing Jin | 2013 | Molecular Plant2013，6，2： | 12 |
| 2 | Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825 and activating defense-related genes in Arabidopsis显示文摘Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato(Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs(mi RNAs) that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor(TIR)-nucleotide binding site(NBS) and leucine-rich repeat(LRR)type resistance(R) genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted. | Dongdong Niu Jing Xia Chunhao Jiang Beibei Qi Xiaoyu Ling Siyuan Lin Weixiong Zhang Jianhua Guo Hailing Jin Hongwei Zhao | 2016 | Journal of Integrative Plant Biology2016，58，4： | 6 |
| 3 | Effective methods for isolation and purification of extracellular vesicles from plants显示文摘Plant extracellular vesicles(EVs)play critical roles in the cross-kingdom trafficking of molecules from hosts to interacting microbes,most notably in plant defense responses.However,the isolation of pure,intact EVs from plants remains challenging.A variety of methods have been utilized to isolate plant EVs from apoplastic washing fluid(AWF).Here,we compare published plant EV isolation methods,and provide our recommended method for the isolation and purification of plant EVs.This method includes a detailed protocol for clean AWF collection from Arabidopsis thaliana leaves,followed by EV isolation via differential centrifugation.To further separate and purify specific subclasses of EVs from heterogeneous vesicle populations,density gradient ultracentrifugation and immunoaffinity capture are then utilized.We found that immunoaffinity capture is the most precise method for specific EV subclass isolation when suitable specific EV biomarkers and their corresponding antibodies are available.Overall,this study provides a guide for the selection and optimization of EV isolation methods for desired downstream applications. | Yifan Huang Shumei Wang Qiang Cai Hailing Jin | 2021 | Journal of Integrative Plant Biology2021，63，12： | 5 |
| 4 | Enhancement of transfection efficiency for HeLa cells via incorporating arginine moiety into chitosan显示文摘Arginine-rich peptides have attracted considerable attention due to their distinct internalization mechanism. It was reported that arginine and guanidino moieties were able to translocate through cell membranes and played a critical role in the process of membrane permeation. In this work, arginine was conjugated to the backbone of chitosan to form a novel chitosan derivative, arginine modified chitosan (Arg-CS). Arg-CS/DNA complexes were prepared according to the method of coacervation process. The physicochemical properties of Arg-CS and Arg-CS/DNA complexes were characterized and the transfection activity and efficiency mediated by Arg-CS/DNA complexes were investigated taking HeLa cells as target cells. Arg-CS was characterized by FTIR and 13C NMR. Arg-CS/DNA polye- lectrolyte complexes were investigated by agarose gel retardation, dynamic light scattering (DLS) and atomic force microscopy (AFM). The results revealed that the Arg-CS/DNA complexes started to form at N/P ratio of 2:1, and the size of particles varied from 100 to 180 nm. The cytotoxicity of Arg-CS and their complexes with plasmid DNA were determined by MTT assay for HeLa cells, and the results suggested that Arg-CS/DNA complexes were slightly less toxic than Arg-CS. Moreover, the derivative alone and their complexes showed significantly lower toxicity than PEI and PEI/DNA complexes, respectively. Taking HeLa cells as target cells and using pGL3-control as reporter gene, the luciferase expression mediated by Arg-CS was greatly enhanced to about 100 folds compared with the luciferase expression mediated by chitosan at different pH media. These results suggest that Arg-CS is a promising candi- date as a safe and efficient vector for gene delivery and transfection. | ZHU DunWan ZHANG HaiLing BAI JinGen LIU WenGuang LENG XiGang SONG CunXian YANG Jian LI XiaoWei JIN Xu SONG LiPing LIU LanXia LI XiuLan ZHANG Yang YAO KangDe | 2007 | Chinese Science Bulletin2007，52，23： | 4 |
| 5 | Comparison of Clinical and Epidemiological Characteristics of Asymptomatic and Symptomatic SARS-CoV-2 Infection in Children显示文摘To understand the epidemiological and clinical features of the symptomatic and asymptomatic pediatric cases of COVID-19,we carried out a prospective study in Shanghai during the period of January 19 to April 30,2020.A total of 49 children(mean age 11.5±5.12 years)confirmed with SARS-CoV-2 infection were enrolled in the study,including 11(22.4%)domestic cases and 38(77.6%)imported cases.Nine(81.8%)local cases and 12(31.6%)imported cases had a definitive epidemiological exposure.Twenty-eight(57.1%)were symptomatic and 21(42.9%)were asymptomatic.Neither asymptomatic nor symptomatic cases progressed to severe diseases.The mean duration of viral shedding for SARS-CoV-2 in upper respiratory tract was 14.1±6.4 days in asymptomatic cases and 14.8±8.4 days in symptomatic cases(P>0.05).Forty-five(91.8%)cases had viral RNA detected in stool.The mean duration of viral shedding in stool was 28.1±13.3 days in asymptomatic cases and 30.8±18.6 days in symptomatic participants(P>0.05).Children<7 years shed viral RNA in stool for a longer duration than school-aged children(P<0.05).Forty-three(87.8%)cases had seropositivity for antibodies against SARS-CoV-2 within 1–3 weeks after confirmation with infection.In conclusion,asymptomatic SARS-CoV-2 infection may be common in children in the community during the COVID-19 pandemic wave.Asymptomatic cases shed viral RNA in a similar pattern as symptomatic cases do.It is of particular concern that asymptomatic individuals are potentially seed transmission of SARS-CoV-2 and pose a challenge to disease control. | Jiehao Cai Xiangshi Wang Jun Zhao Yanling Ge Jin Xu He Tian Hailing Chang Aimei Xia Jiali Wang Jinqiang Zhang Zhongqiu Wei Jingjing Li Chuning Wang Jianshe Wang Qirong Zhu Xiaowen Zhai Mei Zeng | 2020 | Virologica Sinica2020，35，6： | 4 |
| 6 | Antidepressant effect of Chaibu-Shugan-San extract and its constituent in rat models of depression 显示文摘 | Sun-Hee Kim Jin Hail Dae-Hyum Seog | 2005 | Life Sciences2005，7，6： | 1 |
| 7 | Multifunctionality and diversity within the plant MYB-gene family 显示文摘 | Jin Hailing Cathie Martin | 1999 | Plant Molecular Bi- ology1999，41，： | 1 |
| 8 | Efects on the Early Development of the Pearl Sac by Cytochrome c,Yolk Lecithin and Polyvinyplyrro显示文摘EfectsontheEarlyDevelopmentofthePearlSacbyCytochromec,YolkLecithinandPolyvinyplyroZhengJunying,ZhangMuxian,LiuTingColegeofLif... | Jin Hailing Qi Yipeng Jin Tianquan Mallam Nockjoshua Huang Yongxiu | 1997 | Wuhan University Journal of Natural Sciences1997，2，2： | 1 |
| 9 | Bacteria-responsive microRNAs regulate plant innate immunity by modulating plant hormone networks显示文摘 | Weixiong Zhang Shang Gao Xiang Zhou Padmanabhan Chellappan Zheng Chen Xuefeng Zhou Xiaoming Zhang Nyssa Fromuth Gabriela Coutino Michael Coffey Hailing Jin | 2011 | Plant Molecular Biology (-)2011，，1： | 1 |
| 10 | Virus- induced gene silencing in Solanum species 显示文摘 | Brigneti G MartiwHerndndez A M Jin Hailing | 2004 | The Plant Jour- nal2004，39，2： | 1 |
| 11 | Multifunctionality and diversity within the plant MYB-gene family显示文摘 | Hailing Jin Cathie Martin | 1999 | Plant Molecular Biology1999，，5： | 1 |
| 12 | Arabidopsis Argonaute 2 Regulates Innate Immunity via miRNA393 ? -Mediated Silencing of a Golgi-Localized SNARE Gene, MEMB12显示文摘 | Xiaoming Zhang Hongwei Zhao Shang Gao Wei-Chi Wang Surekha Katiyar-Agarwal Hsien-Da Huang Natasha Raikhel Hailing Jin | 2011 | Molecular Cell2011，，3： | 1 |
| 13 | Mild Cytokine Elevation, Moderate CD4^+ T Cell Response and Abundant Antibody Production in Children with COVID-19显示文摘Children with Coronavirus Disease 2019(COVID-19) were reported to show milder symptoms and better prognosis than their adult counterparts, but the difference of immune response against SARS-CoV-2 between children and adults hasn’t been reported. Therefore we initiated this study to figure out the features of immune response in children with COVID-19.Sera and whole blood cells from 19 children with COVID-19 during different phases after disease onset were collected.The cytokine concentrations, SARS-CoV-2 S-RBD or N-specific antibodies and T cell immune responses were detected respectively. In children with COVID-19, only 3 of 12 cytokines were increased in acute sera, including interferon(IFN)-cinduced protein 10(IP10), interleukin(IL)-10 and IL-16. We observed an increase in T helper(Th)-2 cells and a suppression in regulatory T cells(Treg) in patients during acute phase, but no significant response was found in the IFN-cproducing or tumor necrosis factor(TNF)-a-producing CD8?T cells in patients. S-RBD and N IgM showed an early induction, while S-RBD and N IgG were prominently induced later in convalescent phase. Potent S-RBD IgA response was observed but N IgA seemed to be inconspicuous. Children with COVID-19 displayed an immunophenotype that is less inflammatory than adults, including unremarkable cytokine elevation, moderate CD4?T cell response and inactive CD8?T cell response, but their humoral immunity against SARS-CoV-2 were as strong as adults. Our finding presented immunological characteristics of children with COVID-19 and might give some clues as to why children develop less severe disease than adults. | Ran Jia Xiangshi Wang Pengcheng Liu Xiaozhen Liang Yanling Ge He Tian Hailing Chang Hao Zhou Mei Zeng Jin Xu | 2020 | Virologica Sinica2020，35，6： | 1 |
| 14 | Multifunctionality and diversity within the plant MYB-gene family显示文摘 | Hailing Jin Cathie Martin | 1999 | Plant Molecular Biology1999，，5： | 1 |
| 15 | Multifunctionality and diversity within the plant MYB-gene family显示文摘 | Hailing Jin Cathie Martin | 1999 | Plant Molecular Biology1999，，5： | 1 |
| 16 | Antidepressant effect of Chaibu-Shugan-San extract and its constituent in rat models of depression显示文摘 | Sun-Hee Kim Jin HaIl Dae-Hyum Seog | 2005 | Life Sciences2005，7，6： | 1 |
| 17 | Antidepressant effect of Chaibu-Shugan-San extract and its constituent in rat models of depression显示文摘 | Sun- Hee Kim Jin HaIl Dae-Hyum Seog | 2005 | Life Sciences2005，76，： | 1 |
| 18 | Multifunctionality and diversity within the plant MYB-gene family显示文摘 | Hailing Jin Cathie Martin | 1999 | Plant Molecular Biology1999，，5： | 1 |
| 19 | Enhanced thermoelectric properties of NbCoSn half-Heuslers through in-situ nanocrystallization of amorphous precursors during the consolidation process显示文摘Tailoring nanostructures is a general approach used to obtain enhanced thermoelectric properties for halfHeusler compounds because the wide areas of grain and phase boundaries could be scattering centers that lower lattice thermal conductivity.However,a common fabrication method based on the sintering of crystalline precursors crushed from as-cast alloy ingots has limitations in obtaining a homogeneous microstructure without microsized impurity phases,owing to residual elemental segregation from casting.In this study,we used amorphous NbCoSn alloys as a precursor for the sintered specimen to obtain a homogeneous NbCoSn bulk specimen without microsized impurity phases and segregation,which led to the enhanced Seebeck coefficient due to the high purity of the half-Heusler phase after crystallization.Moreover,superplasticity originating from amorphous features enabled the powders to be largely deformed during the sintering process,even at a low sintering temperature(953 K).This resulted in less oxidation at both,the grain boundary and the interior,as the O diffusion pathway was blocked during the sintering process.As a result,the NbCoSn0.95Sb0.05 specimen using an amorphous precursor exhibited an enhanced zT of 0.7,due to the increase in the power factor and a decrease in lattice thermal conductivity compared to the specimen using a crystalline precursor. | Chanwon Jung Kyuseon Jang Hail Park Jeongin Jang Hanhwi Jang Byungchul Kang Kitae Park Siyuan Zhang Ruben Bueno Villoro SuDong Park Ho Jin Ryu Yeon Sik Jung Min-Wook Oh Christina Scheu Seong-Hoon Yi Pyuck-Pa Choi | 2023 | Journal of Materials Science & Technology2023，，34： | 0 |
| 20 | Mechanisms of Small RNA Generation from Cis-NATs in Response to Environmental and Developmental Cues显示文摘真核细胞的染色体的一个大比例从 DNA 的积极、否定的海滨被抄录并且可以因此产生重叠感觉和 antisense 抄本。一些这些所谓的自然 antisense 抄本(NAT ) 可能被共同调整。当重叠感觉和 antisense 抄本响应各种各样的发展、环境的暗示在一样的房间同时被表示时;他们可以形成双 stranded RNA,它能被小 RNA 生物的续生说机械认出并且处理了成小介入 RNA (siRNAs ) 。cis-NAT-derived siRNAs (nat-siRNAs ) 在植物,动物,和真菌是在场的。在植物, nat-siRNAs 的存在不仅被北污点和基因分析,而且由有在 cis-NATs 的重叠区域和在植物的产生 siRNA cis-NATs 的 19%29% 的 siRNAs 的全面六倍的丰富的事实支持仅仅在他们的重叠区域产生 siRNAs。Silencing 由 nat-siRNAs 调停了是为调整 cis-NATs 的表示的机制之一。这评论集中于与 nat-siRNAs 的生物的续生说机制以及规定和察觉有关的挑战性的问题。包括直接 RNA 定序并且海滨特定的 RNA 定序,为检测 cis-NATs 的新技术的优点和限制也被讨论。 | Xiaoming Zhang Yifan Lii Zhigang Wu Anton Polishko Huiming Zhang Viswanathan Chinnusamy Stefano Lonardi Jian-Kang Zhu Renyi Liu Hailing Jin | 2013 | Molecular Plant2013，6，3： | 0 |
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