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| 1 | Immune response,stress resistance and bacterial challenge in juvenile rainbow trouts Oncorhynchus mykiss fed diets containing chitosan-oligosaccharides显示文摘Effects of dietary supplementation of chitosan-oligosaccharides(COS)on the growth performance,immune response,stress resistance,and disease resistance of juvenile rainbow trout Oncorhynchus mykiss were studied.Four experimental diets containing 0,20,40,or 60 mg/kg COS(COS0,COS20,COS40,and COS60,respectively)were fed to juvenile rainbow trout(initial weight=5.2±0.3 g)for 8 weeks.By the end of the feeding trial,representative groups of fish from each dietary treatment were challenged with stressor(30 sec air exposure)and pathogen exposure(intraperitoneal injection with Aeromonas hydrophila).Results showed that supplementation of COS in diets did not affect production performance and body composition of rainbow trout.However,fish fed the COS40 diet demonstrated improved phagocytic activities,respiratory burst activities and decreased serum cortisol level.Additionally,survival following A.hydrophila challenge was significant higher among fish fed the COS-supplemented feeds,although there was no difference based on the level of supplementation.The present study suggests that COS can be used as an immuno-stimulant in rainbow trout feeds. | Lin LUO Xuefeng CAI Chuan HE Min XUE Xiufeng WU Haining CAO | 2009 | Current Zoology2009，55，6： | 22 |
| 2 | A regulated digital currency显示文摘The decentralized digital currency Bitcoin is an anonymous alternative to the centralized banking system and enjoys widespread and increasing adoption. Since Bitcoin created, many other electronic currencies have been developed. We propose a protocol for an electronic currency for making anonymous payments that can be supervised by an auditor, who has sole access to the transaction history. Other electronic currencies provide only anonymity, which is convenient for making illegal transactions without regulation. For users, miners, and banks, the transactions of our electronic currency are anonymous, and only auditors can see how it is used. We make use of POW(prove-of-work) technique that allows for distributed decisionmaking within a network, namely the Bitcoin blockchain protocol. We combine the POW and blockchain technology of Bitcoin to give better protection against double-spending attacks. | Yanbing WU Haining FAN Xiaoyun WANG Guangnan ZOU | 2019 | Science China(Information Sciences)2019，62，3： | 4 |
| 3 | Cep57, a NEDDl-binding pericentriolar material component, is essential for spindle pole integrity显示文摘 | Qixi Wu Runsheng He Haining Zhou Albert CH Yu Bo Zhang Junlin Teng Jianguo Chen | 2012 | Cell Research2012，22，9： | 2 |
| 4 | Rubidium and cesium ion adsorption by an ammonium molybdophosphate–calcium alginate composite adsorbent显示文摘 | Xiushen Ye Zhijian Wu Wu Li Haining Liu Quan Li Binju Qing Min Guo Fei Ge | 2009 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2009，，1： | 1 |
| 5 | Gridbased approach for working node selection in wireless sensor networks 显示文摘 | Chen Haining Wu Hongyi Tzeng Nianfeng | 2004 | IEEE Communications Society2004，6，： | 1 |
| 6 | Non-muscle Mlck is required for β-catenin- and FoxOl-dependent downregulation of Cldn5 in IL-1β-mediated barrier dysfunction in brain endothelial cells显示文摘 | Beard RS Jr Haines RJ Wu KY | 2014 | J Cell Sci2014，127，： | 1 |
| 7 | Protein tyrosine kinase 6 mediates TNFc~-induced endothelial barrier dysfunction 显示文摘 | Haines RJ Beard RS Jr Wu MH | 2015 | Biochem Biophys Res Commun2015，456，1： | 1 |
| 8 | Adsorption kinetics of an organic dye by wet hybrid gel monoliths显示文摘 | Haining Liu Laijiang You Xiushen Ye Wu Li Zhijian Wu | 2008 | Journal of Sol - Gel Science and Technology2008，，3： | 1 |
| 9 | Iodide Adsorption onto Three Organic-Inorganic Composite Adsorbents显示文摘 | Huifang Zhang Tan Guo Quan Li Haining Liu Xiushen Ye Zhijian Wu | 2012 | Adsorption Science & Technology2012，，5： | 1 |
| 10 | Adsorption of iodide ions on a calcium alginate–silver chloride composite adsorbent显示文摘 | Huifang Zhang Xiaolei Gao Tan Guo Quan Li Haining Liu Xiushen Ye Min Guo Zhijian Wu | 2011 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2011，，1： | 1 |
| 11 | Research progress on mechanisms about the effect of ionic strength on adsorption显示文摘 | Wu Zhijian Liu Haining Zhartg Huifang | 2012 | Environment Chemistry2012，29，6： | 1 |
| 12 | Two novel antimicrobial peptides from centipede venoms显示文摘 | Kanfu Peng Yi Kong Lei Zhai Xiongfei Wu Peng Jia Jingze Liu Haining Yu | 2009 | Toxicon2009，，2： | 1 |
| 13 | Overview of the LAMOST survey in the first decade显示文摘The Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),also known as the Guoshoujing Telescope,is a major national scientific facility for astronomical research located in Xinglong,China.Beginning with a pilot survey in 2011,LAMOST has been surveying the night sky for more than 10 years.The LAMOST survey covers various objects in the Universe,from normal stars to peculiar ones. | Hongliang Yan Haining Li Song Wang Weikai Zong Haibo Yuan Maosheng Xiang Yang Huang Jiwei Xie Subo Dong Hailong Yuan Shaolan Bi Yaoquan Chu Xiangqun Cui Licai Deng Jianning Fu Zhanwen Han Jinliang Hou Guoping Li Chao Liu Jifeng Liu Xiaowei Liu Ali Luo Jianrong Shi Xuebing Wu Haotong Zhang Gang Zhao Yongheng Zhao | 2022 | The Innovation2022，3，2： | 0 |
| 14 | Diagnosis and Extinguishment of Camel Rabies显示文摘The diagnosis process and extinguishment measures of a case of camel rabies was introduced from the aspects of medical history,clinical examination,emergency and extinguishment measures,in order to provide some references for clinical veterinarians. | Wu Xinhua Ma Liqin Wu Yawen Wang Jinxiang Zhou Haining Liu Chaolong Wen Jianqiang | 2016 | Animal Husbandry and Feed Science2016，8，6： | 0 |
| 15 | Enzymatic pretreatment mitigates the dissemination of antibiotic resistance genes via regulating microbial populations and gene expressions during food waste fermentation显示文摘Food waste(FW)has been recognized as essential reservoir for resource recovery via anaerobic fermentation,which could also bring the potential risk of antibiotic resistance genes(ARGs)dissemination.Although the structural deficiency of FW could be stimulated by enzymatic pretreatment to enhance fermentation efficiency,the influences of enzymatic pretreatment on ARGs fate and microbial metabolic pathways involved in ARGs dissemination have rarely been reported.This work proved that enzymatic pretreatment could effectively decrease the total abundance of ARGs(reduced by 13.8%-24.5%)during long-term FW fermentation.It was found that enzymatic pretreatment significantly reduced the ARGs belonging to the efflux pump,which might be ascribed to its ability to increase membrane permeability.Furthermore,enzymatic pretreatment was in favor of reducing microbial diversity and various potential ARGs host(e.g.,Methanosarcina,Clostridium,Prevotella,Parabacteroides).Also,this pretreatment remarkably up-regulated the genetic expressions involved in ABC transporter(e.g.,eryF and mntA)and down-regulated the genetic expressions that participated in DNA replication,two-component systems(e.g.,uphA and cckA),and quorum sensing(e.g.,rpfF and lsrG),thereby decreasing ARGs transmission.This study would expand the insight of the influences of pretreatment method on ARGs fate during FW fermentation,and offer practical guidance on the sustainable management of FW. | Yang Wu Wanying Hu Haining Huang Xiong Zheng Lei Dong Yinguang Chen | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 16 | Molecular Marker Techniques Using Single Primers and Their Advances显示文摘Molecular marker techniques have been widely applied in the fields of genetic diversity analysis,germplasm resources identification,molecular fingerprint and genetic linkage map construction,QTL mapping and molecular assisted breeding.On the basis of stating the concept of molecular marker techniques based on single primer amplification reactions,this study focused on the sorting and induction of single-primer molecular marker techniques,and expounded their derivative development.Finally,the application prospect and future expectation of single-primer molecular marker techniques were described in detail.The purpose of this study was to clarify the types of molecular marker techniques based on single primer amplification reactions,so that researchers can quickly and conveniently select molecular marker techniques according to their own specific scientific research conditions. | Junxian LIU Jing LIU Jing JIANG Zhuqiang HAN Xiumei TANG Lihang QIU Ruichun ZHONG Liangqiong HE Haining WU Zhipeng HUANG Ronghua TANG Faqian XIONG | 2021 | Agricultural Biotechnology2021，10，2： | 0 |
| 17 | Boron separation by adsorption and flotation with Mg-Al-LDHs and SDBS from aqueous solution显示文摘Layered double hydroxides(LDHs)have been shown to be effective adsorbents for boron.However,solid-liquid separation is still a problem when separating boron from industrial radioactive waste liquid.In this research,three types of Mg-Al-LDHs including Mg-Al-LDH(NO_(3)^(-)),Mg-Al-LDH(Cl^(-))and Mg-Al-LDH(SO_(4)^(2-))were applied to adsorb boron,and moreover sodium dodecylbenzenesulfonate(SDBS)was used to float the LDH particles from aqueous solution after boron adsorption.The results showed that 60 min was sufficient for the equilibrium adsorption of the three LDHs.The boron adsorption capacity of three LDHs was determined as follows:Mg-Al-LDH(NO_(3)^(-))>Mg-Al-LDH(Cl^(-))>Mg-Al-LDH(SO_(4)^(2-)),and was 2.0,0.98 and 0.2 mmol·g^(-1),each ranging from 0 to 80 mmol·L^(-1)with the initial boron concentration.The efficiency of boron removal by Mg-Al-LDH(NO_(3)^(-))and SDBS can reach up to 89.7%.Furthermore,the boron flotation mechanism of SDBS and LDHs has been studied,since SDBS as a flotation agent can react with LDHs and penetrate into the interlayer of LDHs in addition to electrostatic attraction.Therefore,LDHs in solution can be floated onto the foam layer to be separated from the solution,and the clarified solution was obtained.The method is simple and promising for boron removal from aqueous solution. | Chun Bai Huifang Zhang Qinglong Luo Xiushen Ye Haining Liu Quan Li Jun Li Zhijian Wu | 2023 | Chinese Journal of Chemical Engineering2023，61，9： | 0 |
| 18 | Preparation of Silver Nanoparticles Film and the Adsorption of Iodide显示文摘Iodine and iodine compounds are widely applied in medicaments,dyes,energy materials,food additives etc.The development and utilization of iodine resources have attracted much attention.A large amount of iodide | GAO Can HU Yaoqiang ZHANG Huifang YE Xiushen LI Quan LIU Haining WU Zhijian | 2014 | Acta Geologica Sinica(English Edition)2014，88，S1： | 0 |
| 19 | Illumining phase transformation dynamics of vanadium oxide cathode by multimodal techniques under operando conditions显示文摘Subtle structural changes during electrochemical processes often relate to the degradation of electrode materials.Characterizing the minute-variations in complementary aspects such as crystal structure,chemical bonds,and electron/ion conductivity will give an in-depth understanding on the reaction mechanism of electrode materials,as well as revealing pathways for optimization.Here,vanadium pentoxide (V2O5),a typical cathode material suffering from severe capacity decay during cycling,is characterized by in-situ X-ray diffraction (XRD) and in-situ Raman spectroscopy combined with electrochemical tests.The phase transitions of V2O5 within the 0-1 LiN ratio are characterized in detail.The V--O and V-V distances became more extended and shrank compared to the original ones after charge/discharge process,respectively.Combined with electrochemical tests,these variations are vital to the crystal structure cracking,which is linked with capacity fading.This work demonstrates that chemical bond changes between the transition metal and oxygen upon cycling serve as the origin of the capacity fading. | Guobin Zhang Tengfei Xiong Xuelei Pan Yunlong Zhao Mengyu Yan Haining Zhang Buke Wu Kangning Zhao Liqiang Mai | 2019 | Nano Research2019，12，4： | 0 |
| 20 | A Non-toxic and Efficient Method for Extracting DNA and RNA from Peanut显示文摘[Objectives]To establish a non-toxic and efficient method for extracting DNA and total RNA from peanuts and laying a solid foundation for the molecular biology study of peanuts.[Methods]Based on the principle and method of purifying nucleic acids by silica gel adsorption at high salt and low pH condition,a non-toxic and efficient method to extract peanut DNA and total RNA using cetyltrimethyl ammonium bromide(CTAB)extraction solution was designed.The quality and purity of nucleic acids were detected by agarose gel electrophoresis and nucleic acids protein analyzer,respectively.The quality of DNA was further verified by enzyme digestion and PCR amplification using molecular marker techniques.The quality of total RNA was further verified by reverse transcription(RT)-PCR of actin gene and cDNA-SCoT gene differential display technique.[Results]The agarose gel electrophoresis test showed that the peanut DNA extracted by a low-toxic and effective method is free of contamination and degradation.Through the detection by the nucleic acid protein analyzer,the DNA concentration,yield,A260/A280 and A260/A230 of 5 peanut varieties were 419.6-498.2 ng/μL,20.98-24.91μg/g,1.89-1.96 and 2.03-2.28,respectively.The DNA was of high quality and can be completely digested by EcoRI restriction enzymes,and also can be used for SCoT and SRAP molecular marker technology analysis.The RNA extracted from different tissues of peanuts showed no visible DNA bands by non-denaturing agarose gel electrophoresis.The separated 28S bands were brighter than 18S.The ratio of A260/A280 and A260/A230 showed that the RNA quality was good and can be used for reverse transcription,RT-PCR of actin gene and amplification of cDNA-SCoT gene differential display technique.[Conclusions]This experiment established a low-toxic and effective method for extracting DNA and total RNA from peanuts.Compared with traditional methods,this method is more time-saving and cheaper than commercial kits.The most important point is that this method does not use toxic reagents such as phenol,chloroform and isopropanol.Thus,it is expected to be widely applied in molecular biology research. | Jing LIU Congcong WANG Jing JIANG Zhuqiang HAN Xiumei TANG Lihang QIU Liangqiong HE Ruichun ZHONG Zhipeng HUANG Haining WU Ronghua TANG Junxian LIU Faqian XIONG | 2021 | Asian Agricultural Research2021，13，1： | 0 |
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