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| 1 | Inhibition of KIT RNAi mediated with adenovirus in gastrointestinal stromal tumor xenograft显示文摘AIM: To investigate a therapeutic method for gastrointestinal stromal tumor (GIST) based on KIT RNA interference (RNAi) with AdMax adenovirus. METHODS: KIT short hairpin RNA (shRNA), whose lateral sides were decorated with restriction endonuclease sequences, was designed. T 4 DNA ligase catalyzed the joint of the KIT shRNA and the green fluorescent protein-containing PDC316-EGFP-U6 to form PDC316EGFP-U6-KIT. Homologous recombination of AdEGFPU6-KIT was performed with the AdMax system. Heterotopically transplanted GISTs were established in nude mice. AdEGFP-U6-KIT was intratumorally injected. The volume, inhibition ratio of tumor and CD117 expression of GIST graft tumor in nude mice were compared between test and control groups. RESULTS: The length of KIT shRNA was determined to be about 50bp by agarose electrophoresis. Gene se-quencing detected the designed KIT RNAi sequence in PDC316-EGFP-U6-KIT. After transfection with AdEGFPU6-KIT, 293 cells displayed green fluorescence. The physical and infective titers of AdEGFP-U6-KIT were 5 × 10 11 viral particles/mL and 5.67 × 10 7 plaque forming units/mL, respectively. The mean volume of the grafted tumor was significantly smaller in test mice than in control mice (75.3 ± 22.9 mm 3 vs 988.6 ± 30.5 mm 3 , t = -18.132, P < 0.05). The inhibition ratio of the tumors was 59.6% in the test group. CD117 positive expression was evident in two cases (20%) in the test group and 10 cases (100%) in the control group (χ 2 = 10.2083, P < 0.005). CONCLUSION: AdEGFP-U6-KIT is successfully constructed, and KIT RNAi mediated with Admax vector system can effectively inhibit the expression of the KIT gene and the growth of GIST in nude mice. | Tian-Bao Wang Wen-Sheng Huang Wei-Hao Lin HanPing Shi Wen-Guang Dong | 2010 | World Journal of Gastroenterology2010，16，40： | 6 |
| 2 | Nondestructive diagnostics of soluble sugar,total nitrogen and their ratio of tomato leaves in greenhouse by polarized spectra-hyperspectral data fusion显示文摘Polarized spectra-hyperspectral data fusion technique was used to estimate the soluble sugar(SS),total nitrogen(N),and their ratio(SS/N),of greenhouse tomato leaves.Fresh tomato leaves of five different growth stages(seedling,flowering,initial fruiting,mid-fruiting and picking stage)and five different nitrogen treatments(severe stress 25%,moderate stress 50%,mild stress 75%,normal 100%,and excess 150%)at every stage were collected for spectra acquisition and SS and N determination.Polarized reflectance spectra were acquired with a polarization reflectance spectrum spectro-goniophotometer system and four polarization degree features were extracted.Hyperspectral data were collected with a hyperspectral imaging system and four reflectance spectrum features and eight image features were extracted.Initially,models were built with polarization degree features,image features,and spectral features respectively.Linear and nonlinear fusion methods were comparatively used for modeling based on normalized data of the three sources.The results suggest that the performances of SS/N models are better than those of N and SS models,and the prediction capability of the Support Vector Machine(SVM)models of N and SS/N are superior to those obtained with single kind feature.This work indicates that the polarized spectrum-hyperspectral multidimensional information detecting method can feasibly judge the tomato nutrient stress conditions.Multi-features data fusion analysis technique can enhance the prediction accuracy of spectral diagnostics technology in precision agriculture. | Wenjing Zhu Jinyang Li Lin Li Aichen Wang Xinhua Wei Hanping Mao | 2020 | International Journal of Agricultural and Biological Engineering2020，13，2： | 5 |
| 3 | Studies on the Effects of the Immunosuppressant FK-506 on the High-risk Corneal Graft Rejection显示文摘Purpose: To evaluate the clinical efficacy of FK-506 on suppressing high-risk cornea transplantation rejection.Methods: In a randomized controlled clinical trial, 56 eyes of 56 patients with high-risk keratoplasty (including total corneal transplantation TCT, total corneal transplantation with circular lamellar sclera CST, vascularization corneal transplantation and corneal retransplantation) were divided into the experimental group and the control group(each with 28 eyes).The experimental group was treated by FK-506 eyedrops (0.5 mg/ml) and TobraDex eyedrops, compared with the control group that was treated by 1% CsA eyedrops and TobraDex eyedrops. In the average 8.1-month follow-up period, the visual acuity, graft transparent duration and Rejection Index (RI) of grafts were observed. Results: In the follow-up period, the graft rejection rate of the experimental and the control group was 63.6% and 95.2% respectively (χ2=4. 72, P < 0. 05) with significant difference.Conclusions: The local application of FK-506 suppressed effectively the graft rejection of corneal transplantation of the patients at high risk. | Minhua Wang, Yuesheng Lin, Jiaqi Chen, Yongming Liu, Hanping Xie, Chengtian YeZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | 眼科学报2002，18，3： | 3 |
| 4 | Application of Complex-valued Wavelet Transform to Image Matching显示文摘 | Jiang Hanping Wang Jianmin | 2001 | Journal of Jiangxi Institute of Education (Natural Science)2001，21，6： | 1 |
| 5 | Anomaly secure detection methods by analyzing dynamic characteristics of the network traffic in cloud communications显示文摘 | Wei Xiong Hanping Hu Naixue Xiong Laurence T. Yang Wen-Chih Peng Xiaofei Wang Yanzhen Qu | 2013 | Information Sciences2013，，： | 1 |
| 6 | Separation-enrichment method for airborne disease spores based on microfluidic chip显示文摘Airborne diseases are likely to cause crop yield reduction,which has aroused widespread concern.In this study,a two-stage separation-enrichment structure microfluidic chip with a compound field for separation and enrichment of the greenhouse crops airborne disease spores directly from gas flow was developed.The chip is mainly composed of three parts:arc structure pretreatment channel,semicircular electrode structure and collection tank.COMSOL 5.1 software was used to simulate the designed microfluidic chip.30μm particles were used to represent P.xanthii spores,25μm particles were used to represent P.cubensis spores,and 16μm particles were used to represent B.cinerea spores.The simulation results showed that the separation and enrichment efficiency of 16μm particles,25μm particles,and 30μm particles was 88%,91%,and 94%,respectively.The experimental verification results were observed under a microscope.The results showed that the separation and enrichment efficiency of B.cinereal spores,P.cubensis spores and P.xanthii spores was 75.7%,83.8%and 89.4%,respectively.As a result,the designed microfluidic chip can be used to separate and enrich the spores of airborne diseases of greenhouse crops,which can provide a basis for the research of real-time monitoring technology for greenhouse airborne diseases. | Yafei Wang Xiaodong Zhang Ning Yang Guoxin Ma Xiaoxue Du Hanping Mao | 2021 | International Journal of Agricultural and Biological Engineering2021，14，5： | 1 |
| 7 | Assessment of pyrolysis polygeneration of biomass based on major components: product characterization and elucidation of degradation pathways显示文摘 | Xin Shanzhi Yang Haiping Chen Yingquan Wang Xianhua Chen Hanping | 2013 | Fuel2013，113，： | 1 |
| 8 | Allicin protects against myocardial apoptosis and fibrosis in streptozotocin-induced diabetic rats显示文摘 | LIU Yang QI Hanping WANG Ye | 2012 | Phytomedicine2012，19，： | 1 |
| 9 | "Partial pressures" of humid air in wide pressure and temperature ranges显示文摘在潮湿的空中的部分压力是科学家非常担心的一个问题,没有令人满意的答案迄今为止被发现了。这份报纸建议一个新奇方法在潮湿的空中获得水蒸汽和干燥空气的部分压力。建议方法获得的结果与由道尔顿部分压力法律获得了那相当不同。水蒸汽和干燥空气的基本行为在宽压力和温度范围深入地被学习。半渗透的膜模型被建议并且申请浸透并且不饱和的潮湿的空气。改进因素被开发到份量上描述在不一样的分子之间的相互作用的大小。一发现是在浸透的潮湿的空中的水蒸汽的部分压力等于 P s,而非(f ? ?????? 吗?? | Zidong WANG Hanping CHEN Shilie WENG | 2013 | Frontiers in Energy2013，7，4： | 1 |
| 10 | Experimental study on the effects of shear induced structure in a drag-reducing surfactant solution flow显示文摘 | Zhang Hongxia Wang Dezhong Chen Hanping | 2009 | Archive of Applied Mechonics2009，79，8： | 1 |
| 11 | A stochastic model for workflow QoS evaluation显示文摘 | Xia Yun-ni Wang Hanp Huang Y | 2006 | Scientific Programming2006，14，34： | 1 |
| 12 | Significance of hypoxia-inducible factor?1α expression with atrial fibrosisin rats induced with isoproterenol显示文摘 | Fangju Su Weize Zhang Yongqing Chen Ling Ma Hanping Zhang Fei Wang | 2014 | Experimental and Therapeutic Medicine2014，，6： | 1 |
| 13 | Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis显示文摘 | ZHANG YUNJIAN SHI HANPING WANG WENJIAN | 2011 | Journal of Vascular Surgery2011，53，3： | 1 |
| 14 | Mooren^s ulcer inChina:a study of clinical characteristics and treatment 显示文摘 | Jiaqi Chen Hanping Xie Zhen Wang | 2000 | Br JOphthalmol2000，84，： | 1 |
| 15 | Status and conservation of reeves shad resources in China显示文摘 | WANG Hanping | 1996 | ICLARM Q1996，19，： | 1 |
| 16 | On elementary submodels of a lattic-valued model for language L(Q)显示文摘 | WANG Hanping XIE Huiyang | 2001 | Universities Pekinensis(Natural Science)2001，37，5： | 1 |
| 17 | The influence of microwave dying on biomass pyrolysis 显示文摘 | XIANHUA WANG HANPING CHEN KAI LUO | 2008 | Energy & Fuel2008，22，1： | 1 |
| 18 | Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China显示文摘 | Chengtai Yang Ling Cao Weimin Wang Yi Yang Khalid Abbas Binlun Yan Hanping Wang Li Su Yuhua Sun Huanling Wang | 2009 | Environmental Biology of Fishes2009，，1： | 1 |
| 19 | Narrow Frequency and Sharp Angular Defect Mode in One-Dimensional Photonic Crystals from a Photonic Heterostructure显示文摘 | LIANG G Q HANP WANG H Z | 2004 | Optics Letters2004，29，2： | 1 |
| 20 | Asymmetri electrowetting moving droplets by a square wave显示文摘 | Shih-Kang Fan Hanping Yang Tsu Te Wang Wensyang Hsu | | 0，，： | 1 |
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