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| 1 | Gentiopicroside targets PAQR3 to activate the PI3K/AKT signaling pathway and ameliorate disordered glucose and lipid metabolism显示文摘The obstruction of post-insulin receptor signaling is the main mechanism of insulin-resistant diabetes.Progestin and adipoQ receptor 3(PAQR3),a key regulator of inflammation and metabolism,can negatively regulate the PI3 K/AKT signaling pathway.Here,we report that gentiopicroside(GPS),the main bioactive secoiridoid glycoside of Gentiana manshurica Kitagawa,decreased lipid synthesis and increased glucose utilization in palmitic acid(PA) treated HepG2 cells.Additionally,GPS improved glycolipid metabolism in streptozotocin(STZ) treated high-fat diet(HFD)-induced diabetic mice.Our findings revealed that GPS promoted the activation of the PI3 K/AKT axis by facilitating DNA-binding protein 2(DDB2)-mediated PAQR3 ubiquitinated degradation.Moreover,results of surface plasmon resonance(SPR),microscale thermophoresis(MST) and thermal shift assay(TSA) indicated that GPS directly binds to PAQR3.Results of molecular docking and cellular thermal shift assay(CETSA) revealed that GPS directly bound to the amino acids of the PAQR3 NH2-terminus including Leu40,Asp42,Glu69,Tyr125 and Ser129,and spatially inhibited the interaction between PAQR3 and the PI3 K catalytic subunit(P110α) to restore the PI3 K/AKT signaling pathway.In summary,our study identified GPS,which inhibits PAQR3 expression and directly targets PAQR3 to restore insulin signaling pathway,as a potential drug candidate for the treatment of diabetes. | Haiming Xiao Xiaohong Sun Zeyuan Lin Yan Yang Meng Zhang Zhanchi Xu Peiqing Liu Zhongqiu Liu Heqing Huang | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 7 |
| 2 | Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material显示文摘Stem cell-based therapy has been used to treat ischaemic heart diseases for two decades.However,optimal cell types and transplantation methods remain unclear.This study evaluated the therapeutic effects of human umbilical cord mesenchymal stem cell(hUCMSC)sheet on myocardial infarction(MI).Methods:hUCMSCs expressing luciferase were generated by lentiviral transduction for in vivo bio-luminescent imaging tracking of cells.We applied a temperature-responsive cell culture surface-based method to form the hUCMSC sheet.Cell retention was evaluated using an in vivo bio-luminescent imaging tracking system.Unbiased transcriptional profiling of infarcted hearts and further immunohistochemical assessment of monocyte and macrophage subtypes were used to determine the mechanisms underlying the therapeutic effects of the hUCMSC sheet.Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function,angiogenesis and left ventricular remodelling.Results:When transplanted to the infarcted mouse hearts,hUCMSC sheet significantly improved the retention and survival compared with cell suspension.At the early stage of MI,hUCMSC sheet modulated inflammation by decreasing Mcp1-positive monocytes and CD68-positive macrophages and increasing Cx3cr1-positive non-classical macrophages,preserving the cardiomyocytes from acute injury.Moreover,the extracellular matrix produced by hUCMSC sheet then served as bioactive scaffold for the host cells to graft and generate new epicardial tissue,providing mechanical support and routes for revascularsation.These effects of hUCMSC sheet treatment significantly improved the cardiac function at days 7 and 28 post-MI.Conclusions:hUCMSC sheet formation dramatically improved the biological functions of hUCMSCs,mitigating adverse post-MI remodelling by modulating the inflammatory response and providing bioactive scaffold upon transplantation into the heart.Translational perspective:Due to its excellent availability as well as superior local cellular retention and survival,allogenic transplantation of hUCMSC sheets can more effectively acquire the biological functions of hUCMSCs,such as modulating inflammation and enhancing angiogenesis.Moreover,the hUCMSC sheet method allows the transfer of an intact extracellular matrix without introducing exogenous or synthetic biomaterial,further improving its clinical applicability. | Rui Guo Feng Wan Masatoshi Morimatsu Qing Xu Tian Feng Hang Yang Yichen Gong Shuhong Ma Yun Chang Siyao Zhang Youxu Jiang Heqing Wang Dehua Chang Hongjia Zhang Yunpeng Ling Feng Lan | 2021 | Bioactive Materials2021，6，9： | 6 |
| 3 | Controlled growth and photoluminescence of highly oriented arrays of ZnO nanocones with different diameters显示文摘Large-scale oriented ZnO nanocone arrays were directly grown on zinc substrate through a hydro-thermal reaction of Zn foil with aqueous butylamine solution(3 mol/L) at 100-180 ℃ for 12 h.The syn-thesized products were characterized with X-ray diffraction,Raman spectrum,scanning electron mi-croscopy and transmission electron microscopy.The results showed that the ZnO nanocones were single crystalline with the wurtzite structure and grown along the [0001] direction.The diameter of nanocones is decreased with increasing the reaction temperature.A possible growth mechanism was also proposed to account for the formation of the ZnO nanocone arrays.The photoluminescence spectra of the ZnO nanocone arrays were studied at room temperature,two UV emission bands at 377 and 396 nm assigned to free exciton emission and exciton-exciton collision,respectively,and phonon replicas associated with 2-E2 phonon were observed in the PL spectra. | MA JunHu YANG HeQing SONG YuZhe LI Li XIE XiaoLi LIU RuiNi WANG LinFang | 2009 | Science China(Technological Sciences)2009，52，5： | 4 |
| 4 | Cardiorespiratory Effects of Indoor Ozone Exposure Associated with Changes in Metabolic Profiles among Children:A Repeated-Measure Panel Study显示文摘Ozone is one of the major gaseous pollutants associated with short-term adverse cardiopulmonary effects,even at concentrations below the current indoor air quality limits.However,the underlying biological mechanisms of cardiorespiratory changes with exposure to ozone remain unclear.To further explore molecular linkages between indoor ozone exposure and relevant cardiorespiratory effects,a repeated-measure panel study including 46 schoolchildren was conducted and real-time exposure measurements including ozone were performed inside classrooms every weekday during the study period.Repeated health measurements and urine sample collection were conducted in each participant.Ultra-high-performance liquid chromatography/tandem mass spectrometry and meet-in-metabolite approach were used in metabolomics analysis.Methods including mixed-effect models were adopted to identify metabolites associated with ozone exposure or health indices.Nine metabolites were found to be associated with ozone after mixed-effect model analysis,which are mainly involved in amino acid and bile acid metabolism.Boys may have a greater decrease in bile acid and RNA related metabolites.Four of the nine ozone-related metabolites were also associated with cardiorespiratory function indices.Furthermore,26.67%of the positive association between ozone and heart rate was mediated by cholestane-3,7,12,25-tetrol-3-glucuronide.Exposure to ozone below the current indoor standards was associated with the deteriorated cardiovascular function by disturbing bile acid and endogenous nitric oxide-related oxidation and inflammation,and associated with the exacerbated airway inflammation by reducing GPx-related anti-oxidation.The results provide metabolic evidence of the cardiorespiratory effects of indoor ozone exposure.Indoor ozone pollution should be controlled further,and more attention should be paid to preventing its adverse health effects,especially in children. | Shan Liu Qingyu Huang Xi Zhang Wei Dong Wenlou Zhang Shaowei Wu Di Yang Bingru Nan Jie Zhang Heqing Shen Xinbiao Guo Furong Deng | 2021 | The Innovation2021，2，1： | 1 |
| 5 | 显示文摘 | Yang Heqing Yang Ruili Wan Xiuqin | 2004 | J Crystal Growth2004，261，： | 1 |
| 6 | In Situ Growth of Self-Assembled and Single In203 Nanosheets on the Surface of Indium Grains显示文摘 | YANG Heqing ZhANG Ruigang DONG Hongxing | 2008 | Cryst Growth Des2008，8，9： | 1 |
| 7 | Synthesis of 5-(5-nitro-2-pyridylazo)-2,4-diaminotoluene and its application to spectrophotometric determination of microamounts of palladium显示文摘 | Heqing Yang Guang Zhang Linlin Zhang Genqi Liu Xinhuai Zhang | 1996 | Talanta1996，，5： | 1 |
| 8 | Synthesis of 5-(5-nitro-2-pyridyaz)-2,4-diaminotoluene and its applica-tion to spectrephotometric determination of microamounts of palladium显示文摘 | Heqing Yang Guang Zhang Linlin Zhang | 1996 | Taltanta1996，43，： | 1 |
| 9 | Synthesis of 5-( 5-nitro-2-pyridylazo )-2, 4-diaminotoluene and its application to spectrophotometric determination of micro-amounts of palladium 显示文摘 | Yang Heqing Zhang Guang Zhang Xiaolin | 1996 | Talanta1996，43，： | 1 |
| 10 | Synthesis of 5- ( 5-nitro-2-pyridylazo)-2,4-diaminotoluene and its application to spectrophotometric determination of microamounts of palladium显示文摘 | Yang Heqing Zhang Guang Zhang Xiaolin | 1996 | Talanta1996，43，： | 1 |
| 11 | Synthesis of 5-( 5-nitro-2-pyridylazo)-2, 4-diamin- otoluene and its application to spectrophotometric determination of microamounts of palladium显示文摘 | Yang Heqing Zhang Guang Zhang Linlin | 1996 | Ta- lanta1996，43，5： | 1 |
| 12 | Preparation and photocatalytic activity of hollow ZnSe microspheres via Ostwald ripening显示文摘 | Lihui Zhang Heqing Yang Xiaoli Xie | | 0，，： | 1 |
| 13 | Supercontinuum single-photon detector using multilayer superconducting nanowires显示文摘High-efficiency superconducting nanowire single-photon detectors(SNSPDs), which have numerous applications in quantum information systems, function by using the optical cavity and the ultrasensitive photon response of their ultra-thin superconducting nanowires. However, the wideband response of superconducting nanowires is limited due to the resonance of the traditional optical cavity. Here, we report on a supercontinuum SNSPD that can efficiently detect single photons over an ultra-broad spectral range from visible to mid-infrared light. Our detection approach relies on using multiple cavities with well-separated absorbed resonances formed by fabricating multilayer superconducting nanowires on metallic mirrors with silica acting as spacer layers. Thus, we are able to extend the absorption spectral bandwidth while maintaining considerable efficiency, as opposed to a conventional single-layer SNSPD. Our calculations show that the proposed supercontinuum SNSPD exhibits an extended absorption bandwidth with increased nanowire layers. Its absorption efficiency is greater than 70%over the entire range from 400 to 2500 nm(or 400 to 3000 nm), when using two-layer(or three-layer) nanowires.As a proof of principle, the SNSPD with bilayer nanowires is fabricated based on the proposed detector architecture with simplified geometrical parameters. The detector achieves broadband detection efficiency over 60%from 950 to 1650 nm. This type of detector may replace multiple narrow band detectors in a system and find uses in the emerging and rapidly advancing field of atomic and molecular broadband spectroscopy. | HAO LI YONG WANG LIXING YOU HEQING WANG HUI ZHOU PENG HU WEIJUN ZHANG XIAOYU LIU XIAOYAN YANG LU ZHANG ZHEN WANG XIAOMING XIE | 2019 | Photonics Research2019，7，12： | 1 |
| 14 | Variation of the Energy Field of Longmenshan Fault Zone before the Wenchuan M_S 8. 0 Earthquake显示文摘During the process of preparation and occurrence of a large earthquake,the stress-strain state along the fault zone has close relation with the weak seismicity around the fault zone. The seismic energy release near the fault zone before an earthquake can better reflect the dynamic process of earthquake preparation. Thus,in this paper,the method of natural orthogonal function expansion has been adopted to discuss the time variation about the energy field of the seismic activity along the Longmenshan fault zone before the Wenchuan M_S 8. 0 earthquake,2008. The results show that evident short-term rise changes appeared in the time factors of the typical field corresponding to several key eigenvalues of the energy field along the Longmenshan fault zone before the Wenchuan earthquake,probably being the short-term anomaly message for this earthquake. Through contrastive analysis of earthquake examples such as the 1976 Tangshan earthquake,the authors think that the study of time variation of energy field of seismicity along active fault zone will be helpful for conducting intentional and intensive earthquake monitoring and forecast in active fault regions with high seismic risk based on medium- and long-term earthquake trend judgment. | Yang Mingzhi Ma Heqing | 2012 | Earthquake Research in China2012，26，3： | 0 |
| 15 | Effect of reduction-oxidation treatment on structure and catalytic properties of ordered mesoporous Cu-Mg-Al composite oxides显示文摘Ordered mesoporous Cu–Mg–Al composite oxides were synthesized via the one-pot evaporation-induced self-assembly strategy. Using this method, copper was first homogeneously incorporated into the ordered mesoporous spinel matrix. After H2 reduction treatment,according to X-ray diffraction(XRD) and transmission electron microscopy(TEM) results, copper existed as metallic nanoparticles with the size of 6–10 nm that well decorated the parent mesoporous skeleton. The metallic nanoparticles were then re-oxidized to copper oxide when exposed to air or during CO oxidation reaction at low temperatures. Thus, copper migrated from bulk spinel phase to the surface after the reduction–oxidation treatment. Moreover, the copper on the surface was re-incorporated into the bulk spinel phase by further thermaltreatment at much higher temperature in the presence of air. The correlation between the state of copper in the mesoporous composite oxides and the catalytic performance toward CO oxidation was studied. It was found that copper existed as oxide nanoparticles on the surface of mesoporous Mg–Al skeleton is much more active than that existed as lattice Cu ions in spinel phase. | Jingting Lu Yan Zhang Chengli Jiao Suresh Kumar Megarajan Dong Gu Guocheng Yang Heqing Jiang Chunjiang Jia Ferdi Schiith | 2015 | Science Bulletin2015，60，12： | 0 |
| 16 | Harris Hawks Algorithm Incorporating Tuna Swarm Algorithm and Differential Variance Strategy显示文摘Because of the low convergence accuracy of the basic Harris Hawks algorithm,which quickly falls into the local optimal,a Harris Hawks algorithm combining tuna swarm algorithm and differential mutation strategy(TDHHO)is proposed.The escape energy factor of nonlinear periodic energy decline balances the ability of global exploration and regional development.The parabolic foraging approach of the tuna swarm algorithm is introduced to enhance the global exploration ability of the algorithm and accelerate the convergence speed.The difference variation strategy is used to mutate the individual position and calculate the fitness,and the fitness of the original individual position is compared.The greedy technique is used to select the one with better fitness of the objective function,which increases the diversity of the population and improves the possibility of the algorithm jumping out of the local extreme value.The test function tests the TDHHO algorithm,and compared with other optimization algorithms,the experimental results show that the convergence speed and optimization accuracy of the improved Harris Hawks are improved.Finally,the enhanced Harris Hawks algorithm is applied to engineering optimization and wireless sensor networks(WSN)coverage optimization problems,and the feasibility of the TDHHO algorithm in practical application is further verified. | XU Xiaohan YANG Haima ZHENG Heqing LI Jun LIU Jin ZHANG Dawei HUANG Hongxin | 2023 | Wuhan University Journal of Natural Sciences2023，28，6： | 0 |
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