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| 1 | Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1显示文摘AIM:To evaluate the protective effects of fucoidan on oxidative stress-induced barrier disruption in human intestinal epithelial cells.METHODS:In Caco-2 cell monolayer models,the disruption of barrier function by oxidative stress is mediated by H2O2.The integrity of polarized Caco-2 cell monolayers was determined by measuring the transepithelial resistance(TER)and permeability was estimated by measuring the paracellular transport of FITC-labeled4-kDa dextran(FD4).The protective effects of fucoidan on epithelial barrier functions on polarized Caco-2 cell monolayers were evaluated by TER and FD4 flux.The expression of tight junction(TJ)proteins was assessed using reverse-transcription polymerase chain reaction(RT-PCR)and immunofluorescence staining.RESULTS:Without H2O2treatment,fucoidan significantly increased the TER compared to control(P<0.05),indicating a direct enhancement of intestinal epithelial barrier function.Next,H2O2disrupted the epithelial barrier function in a time-dependent manner.Fucoidan prevented the H2O2-induced destruction in a dosedependent manner.Fucoidan significantly decreased H2O2-induced FD4 flux(P<0.01),indicating the prevention of disruption in paracellular permeability.RTPCR showed that Caco-2 cells endogenously expressed claudin-1 and-2,and occludin and that H2O2reduced the mRNA expression of these TJ proteins.Treatment with fucoidan attenuated the reduction in the expressions of claudin-1 and claudin-2 but not occludin.Immunofluorescence staining revealed that the expression of claudin-1 was intact and high on the cell surface.H2O2disrupted the integrity of claudin-1.Treatment with fucoidan dramatically attenuated the expression of claudin-1.CONCLUSION:Fucoidan enhanced intestinal epithelial barrier function by upregulating the expression of claudin-1.Thus,fucoidan may be an appropriate therapy for the treatment of inflammatory bowel diseases. | Atsushi Iraha Hiroshi Chinen Akira Hokama Takumi Yonashiro Tetsu Kinjo Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2013 | World Journal of Gastroenterology2013，19，33： | 15 |
| 2 | Different natural courses of chronic hepatitis B with genotypes B and C after the fourth decade of life显示文摘AIM: To investigate the different impact of genotypes B and C on the development of liver cirrhosis (LC) among different age groups of patients with chronic hepatitis B (CH-B). METHODS: We examined the outcome of 121 patients with CH-B, divided by age and genotype. Univariate analyses were used to compare different groups. The Cox proportional hazard model was employed to evaluate factors affecting the development of LC. RESULTS: In patients < 30 years old, there were no significant predictors for development of LC. However, in patients ≥ 30 years old, genotype C was the only significant predictor. In the genotype C group, 8 of 12 patients who progressed to LC were 30-49 years old at initial diagnosis of chronic hepatitis (7 patients were positive for HBeAg). In the genotype B group, 4 of 8 patients who developed LC were ≥ 50 years old at initial diagnosis and were HBeAg-negative. CONCLUSION: The rate of development of LC was comparable in patients infected with genotypes B and C when CH-B occurred at < 30 years old. However, CH-B patients infected with genotype C showed poor prognosis if they were 30-49 years old and were positive for HBeAg. Age-specific natural course of CH-B should be considered when patients with CH-B are treated with antiviral drugs. | Tatsuji Maeshiro Shingo Arakaki Takako Watanabe Hajime Aoyama Joji Shiroma Tsuyoshi Yamashiro Tetsuo Hirata Akira Hokama Fukunori Kinjo Tomofumi Nakayoshi Tomokuni Nakayoshi Masashi Mizokami Jiro Fujita Hiroshi Sakugawa | 2007 | World Journal of Gastroenterology2007，13，34： | 7 |
| 3 | Early effects of oral administration of omeprazole and roxatidine on intragastric pH显示文摘Objective:The ideal medication for the treatment of acid-related diseases,e.g.,peptic ulcers,stress-related gastric bleeding,functional dyspepsia,and gastroesophageal reflux disease,should have a rapid onset of action to promote hemostasis and relieve the symptoms.The aim of our study was to investigate the inhibitory effects on gastric acid secretion of a single oral administration of a proton pump inhibitor,omeprazole 20 mg,and an H2-receptor antagonist,roxatidine 75 mg.Methods:Ten Helicobacter pylori-negative male subjects participated in this randomized,two-way crossover study.Intragastric pH was monitored continuously for 6 h after single oral administration of omeprazole 20 mg and roxatidine 75 mg.Each administration was separated by a 7-d washout period.Results:During the 6-h study period,the average pH after administration of roxatidine was higher than that after administration of omeprazole(median:4.45 vs.2.65;P=0.0367).Also during the 6-h study period,a longer duration of maintenance at pH above 2,5,and 6 was observed after administration of roxatidine 75 mg than after administration of omeprazole 20 mg(median:90.6% vs.55.2%,P=0.0284;43.7% vs.10.6%,P=0.0125;40.3% vs.3.3%,P=0.0125;respectively).Conclusions:In Helicobacter pylori-negative healthy male subjects,oral administration of roxatidine 75 mg increased the intragastric pH more rapidly than that of omeprazole 20 mg. | Hiroshi IIDA Shingo KATO Yusuke SEKINO Eiji SAKAI Takashi UCHIYAMA Hiroki ENDO Kunihiro HOSONO Yasunari SAKAMOTO Koji FUJITA Masato YONEDA Tomoko KOIDE Hirokazu TAKAHASHI Chikako TOKORO Ayumu GOTO Yasunobu ABE Noritoshi KOBAYASHI Kensuke KUBOTA Eiji GOTOH Shin MAEDA Atsushi NAKAJIMA Masahiko INAMORI | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，1： | 6 |
| 4 | Imaging pitfalls of pancreatic serous cystic neoplasm and its potential mimickers显示文摘The aim of this article is to clarify diagnostic pitfalls of pancreatic serous cystic neoplasm(SCN) that may result in erroneous characterization. Usual and unusual imaging findings of SCN as well as potential SCN mimickers are presented. The diagnostic key of SCN is to look for a cluster of microcysts(honeycomb pattern), which may not be always found in the center. Fibrosis in SCN may be mistaken for a mural nodule of intraductal papillary mucinous neoplasm(IPMN). The absence of cyst wall enhancement may be helpful to distinguish SCN from mucinous cystic neoplasm. However, oligocystic SCN and branch duct type IPMN may morphologically overlap. In addition, solid serous adenoma, an extremely rare variant of SCN, is difficult to distinguish from neuroendocrine tumor. | Kousei Ishigami Akihiro Nishie Yoshiki Asayama Yasuhiro Ushijima Yukihisa Takayama Nobuhiro Fujita Shunichi Takahata Takao Ohtsuka Tetsuhide Ito Hisato Igarashi Shuji Ikari Catherine M Metz Hiroshi Honda | 2014 | World Journal of Radiology2014，6，3： | 6 |
| 5 | Levovist ultrasonography imaging in intracystic hemorrhage of simple liver cyst显示文摘The differential diagnosis between intracystic hemorrhage and cystadenocarcinoma of the liver is often difficult even with the use of various imaging modalities. A 73-year-old woman was admitted to our hospital with the complaint of right upper quadrant pain. Ultrasonography (US) demonstrated a heterogeneous echogenic cyst measuring 11 cm × 8 cm in size in S2 of the liver, indicated intracystic hemorrhage of simple liver cyst or cystadenocarcinoma, but the differential diagnosis was considerably difficult. Levovist (Schering, Berlin, Germany) US revealed no enhancement of the intracystic structures, suggesting a clot in the case of intracystic hemorrhage. An operation was performed and the resected lesion showed a solitary benign liver cyst, measuring 5.5 cm × 4.7 cm × 8.5 cm containing a large blood clot. The patient had an uneventful recovery after the surgery. Levovist US may play an important role in discrimination between intracystic hemorrhage of simple hepatic cysts and cystadenocarcinoma of the liver. | Tomoyuki Akiyama Masahiko Inamori Satoru Saito Hirokazu Takahashi Masato Yoneda Koji Fujita Toshio Fujisawa Yasunobu Abe Hiroyuki Kirikoshi Kensuke Kubota Michio Ueda Kuniya Tanaka Shinji Togo Norio Ueno Hiroshi Shimada Atsushi Nakajima | 2008 | World Journal of Gastroenterology2008，14，5： | 4 |
| 6 | Shape of Barrett's epithelium is associated with prevalence of erosive esophagitis显示文摘AIM:To test the hypothesis that the shape and length of Barrett's epithelium are associated with prevalence of erosive esophagitis.METHODS:A total study population comprised 869 patients who underwent endoscopy during a health checkup at our hospital.The presence and extent of Barrett's epithelium were diagnosed based on the Prague C & M Criteria.We originally classified cases of Barrett's epithelium into two types based on its shape,namely,flamelike and lotus-like Barrett's epithelium,and into two groups based on its length,its C extent < 2 cm,and ≥ 2 cm.Correlation of shape and length of Barrett's epithelium with erosive esophagitis was examined.RESULTS:Barrett's epithelium was diagnosed in 374 cases(43%).Most of these were diagnosed as shortsegment Barrett's epithelium.The prevalence of erosive esophagitis was significantly higher in subjects with flame-like than lotus-like Barrett's epithelium,and in those with a C extent of ≥ 2 cm than < 2 cm.CONCLUSION:Flame-like rather than lotus-like Barrett's epithelium,and Barrett's epithelium with a longer segment were more strongly associated with erosive esophagitis. | Tomoyuki Akiyama Masahiko Inamori Hiroshi Iida Hiroki Endo Kunihiro Hosono Yasunari Sakamoto Koji Fujita Masato Yoneda Hirokazu Takahashi Tomoko Koide Chikako Tokoro Ayumu Goto Yasunobu Abe Takeshi Shimamura Noritoshi Kobayashi Kensuke Kubota Satoru Saito Atsushi Nakajima | 2010 | World Journal of Gastroenterology2010，16，4： | 4 |
| 7 | Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria显示文摘Objective: To investigate the effect of the roselle calyx extract(RCE)(Hibiscus sabdariffa L.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. Methods:RCE was prepared by soaking roselle calyx powder with ethyl alcohol for 24 h at room temperature. After centrifugation, the extract was lyophilized. Then, the extract was dissolved in phosphate-buffered saline, the p H was adjusted, and the extract was aseptically filtered. We used Streptococcus mutans, Streptococcus sanguinis, Lactobacillus casei, Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in this study. The antibacterial activity of the RCE was determined by treating the cells of these bacteria with the extract for 10 or 20 min at room temperature. The minimum inhibitory concentration(MIC) and minimum bactericidal concentration was determined using the micro dilution method, and the effect of the RCE on the ability to form biofilm was determined using a polystyrene micro plate assay. In addition, we used the WST-1 assay to determine the cytotoxicity of the RCE on HGF, Ca9-22 and KB cells. Results: The RCE had antibacterial activity against oral bacteria used in this study. In particular, most significant antibacterial activity was observed against Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The MIC and minimum bactericidal concentration were 7.2 mg/m L–28.8 mg/m L and 14.4 to >57.6 mg/m L. The RCE had an inhibitory effect on biofilm formation at the MIC and sub-MIC levels. In addition, the RCE had low cytotoxic effects on HGF, Ca9-22 and KB cells. Conclusions: Thus, our results indicate that the RCE may be used for preventing oral diseases. | Herastuti Sulistyani Mari Fujita Hiroshi Miyakawa Futoshi Nakazawa | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，2： | 3 |
| 8 | Outcome of endoscopic mucosal resection for early gastric cancer: review of the Japanese literature显示文摘 | Toshihiro Kojima Adolfo Parra-Blanco Hiroshi Takahashi Rikiya Fujita | 1998 | Gastrointestinal Endoscopy1998，，5： | 3 |
| 9 | NON-STATIONARY STOKES FLOWS UNDER LEAK BOUNDARY CONDITIONS OF FRICTION TYPE显示文摘This paper is concerned with the initial value problem for non-stationary Stokes flows, under a certain non-linear boundary condition which can be called the leak boundary condition of friction type. Theoretically, our main purpose is to show the strong solvability (i.e.,the unique existence of the L2-strong solution) of this initial value problem by means of the non-linear semi-group theory originated with Y. Komura. The method of analysis can be applied to other boundary or interface conditions of friction type. It should be noted that the result yields a sound basis of simulation methods for evolution problems involving these conditions., | Hiroshi Fujita (The Research institute of Educational Development, Tokai University, Shibuya-ku, Tokyo 151-0063, Japan) | 2001 | Journal of Computational Mathematics2001，19，1： | 3 |
| 10 | Image quality and diagnostic performance of free-breathing diffusion-weighted imaging for hepatocellular carcinoma显示文摘AIM To retrospectively evaluate the diagnostic performance of free-breathing diffusion-weighted imaging(FB-DWI) with modified imaging parameter settings for detecting hepatocellular carcinomas(HCCs).METHODS Fifty-one patients at risk for HCC were scanned with both FB-DWI and respiratory-triggered DWI with the navigator echo respiratory-triggering technique(RTDWI).Qualitatively,the sharpness of the liver contour,the image noise and the chemical shift artifacts on each DWI with b-values of 1000 s/mm2 were independently evaluated by three radiologists using 4-point scoring.Wecompared the image quality scores of each observer between the two DWI methods,using the Wilcoxon signed-rank test.Quantitatively,we compared the signal-to-noise ratios(SNRs) of the liver parenchyma and lesion-to-nonlesion contrast-to-noise ratios(CNRs) after measuring the signal intensity on each DWI with a b-factor of 1000 s/mm2.The average SNRs and CNRs between the two DWI methods were compared by the paired t-test.The detectability of HCC on each DWI was also analyzed by three radiologists.The detectability provided by the two DWI methods was compared using Mc Nemar's test.RESULTS For all observers,the averaged image quality scores of FB-DWI were:Sharpness of the liver contour [observer(Obs)-1,3.08 ± 0.81;Obs-2,2.98 ± 0.73;Obs-3,3.54 ± 0.75],those of the distortion(Obs-1,2.94 ± 0.50;Obs-2,2.71 ± 0.70;Obs-3,3.27 ± 0.53),and the chemical shift artifacts(Obs-1,3.38 ± 0.60;Obs-2,3.15 ± 1.07;Obs-3,3.21 ± 0.85).The averaged image quality scores of RTDWI were:Sharpness of the liver contour(Obs-1,2.33 ± 0.65;Obs-2,2.37 ± 0.74;Obs-3,2.75 ± 0.81),distortion(Obs-1,2.81 ± 0.56;Obs-2,2.25 ± 0.74;Obs-3,2.96 ± 0.71),and the chemical shift artifacts(Obs-1,2.92 ± 0.59;Obs-2,2.21 ± 0.85;Obs-3,2.77 ± 1.08).All image quality scores of FB-DWI were significantly higher than those of RT-DWI(P < 0.05).The average SNR of the normal liver parenchyma by FB-DWI(11.0 ± 4.8) was not significantly different from that shown by RT-DWI(11.0 ± 5.0);nor were the lesion-to-nonlesion CNRs significantly different(FB-DWI,21.4 ± 17.7;RT-DWI,20.1 ± 15.1).For all three observers,the detectability of FB-DWI(Obs-1,43.6%;Obs-2,53.6%;and Obs-3,45.0%) was significantly higher than that of RT-DWI(Obs-1,29.1%;Obs-2,43.6%;and Obs-3,34.5%)(P < 0.05).CONCLUSION FB-DWI showed better image quality and higher detectability of HCC compared to RT-DWI,without significantly reducing the SNRs of the liver parenchyma and lesionto-nonlesion CNRs. | Yukihisa Takayama Akihiro Nishie Yoshiki Asayama Kousei Ishigami Daisuke Kakihara Yasuhiro Ushijima Nobuhiro Fujita Ken Shirabe Atsushi Takemura Hiroshi Honda | 2017 | World Journal of Hepatology2017，9，14： | 3 |
| 11 | Effects of increased adrenomedullary activity and taurine in young patientswith borderline hypertension显示文摘 | TOSHIRO FUJITA KATSUYUKi ANDO HIROSHi NODA YASUSHI ITO YUJI SATO | 1987 | Circulation1987，，3： | 2 |
| 12 | Mucosal-associated invariant T cells from induced pluripotent stem cells:A novel approach for modeling human diseases显示文摘Mice have frequently been used to model human diseases involving immune dysregulation such as autoimmune and inflammatory diseases.These models help elucidatethe mechanisms underlying the disease and in the development of novel therapies.However,if mice are deficient in certain cells and/or effectors associated with human diseases,how can their functions be investigated in this species?Mucosal-associated invariant T(MAIT)cells,a novel innate-like T cell family member,are a good example.MAIT cells are abundant in humans but scarce in laboratory mice.MAIT cells harbor an invariant T cell receptor and recognize nonpeptidic antigens vitamin B2metabolites from bacteria and yeasts.Recent studies have shown that MAIT cells play a pivotal role in human diseases such as bacterial infections and autoimmune and inflammatory diseases.MAIT cells possess granulysin,a human-specific effector molecule,but granulysin and its homologue are absent in mice.Furthermore,MAIT cells show poor proliferation in vitro.To overcome these problems and further our knowledge of MAIT cells,we have established a method to expand MAIT cells via induced pluripotent stem cells(iP SCs).In this review,we describe recent advances in the field of MAIT cell research and our approach for human disease modeling with iP SCderived MAIT cells. | Chie Sugimoto Hiroyoshi Fujita Hiroshi Wakao | 2016 | World Journal of Stem Cells2016，8，4： | 2 |
| 13 | Optimum Treatment Strategy for Superficial Esophageal Cancer: Endoscopic Mucosal Resection versus Radical Esophagectomy显示文摘 | Hiromasa Fujita Susumu Sueyoshi Hideaki Yamana Koji Shinozaki Uhi Toh Yuichi Tanaka Takashi Mine Masahiro Kubota Kazuo Shirouzu Atsushi Toyonaga Hiroshi Harada Sigeki Ban Masahide Watanabe Yukihiko Toda Emi Tabuchi Naofumi Hayabuchi Hiroki Inutsuka | 2001 | World Journal of Surgery2001，，4： | 2 |
| 14 | The CagA protein of Helicobacter pylori suppresses the functions of dendritic cell in mice显示文摘 | Hiroshi Tanaka Masaru Yoshida Shin Nishiumi Naomi Ohnishi Kazuki Kobayashi Koji Yamamoto Tsuyoshi Fujita Masanori Hatakeyama Takeshi Azuma | 2010 | Archives of Biochemistry and Biophysics2010，，1： | 2 |
| 15 | PERCIST用于头颈部鳞状细胞癌患者放化疗疗效评价及预后预测显示文摘目的探讨PET实体瘤疗效评价标准(PERCIST)评价头颈部鳞状细胞癌(HNSCC)患者放化疗疗效及预测复发和死亡的价值。方法42例患者(平均年龄63岁,范围20~79岁)在放化疗前及放化疗后约3个月(平均95d,范围70~119d)行脱氧葡萄糖(FDG)-PET/CT显像,其中包括10例鼻咽癌、13例口咽癌、11例下咽癌及8例喉癌。与PERCIST相关的无进展生存时间(PFS)和总生存时间(OS)采用对数秩检验和Cox回归分析进行统计学处理。结果PERCIST确定的完全代谢反应(CMR,即病灶FDG摄取降低至肝脏的平均活度水平以下或病灶FDG摄取与周围组织无明显差别)、部分代谢反应[PMR,即经去脂体质量校正的高峰标准摄取值(SULpeak)下降30%及以上]、稳定代谢反应(SMD,即FDG摄取无明显变化)和病灶代谢增加(PMD,即SULpeak增加30%及以上)的患者数分别为30例(71.4%)、9例(21.4%)、3例(7.1%)和0例。 | 唐军(译) Takayuki Katsuura Kazuhiro Kitajirma Masayuki Fujiwara Tomonori Terada Nobuhiro Uwa Kazuma Noguchi Hiroshi Doi Yukihisa Tamaki Rika Yoshida Tatsuya Tsuchitani Masahiro Fujita Koichiro Yamakado | 2018 | 中华核医学与分子影像杂志2018，38，12： | 2 |
| 16 | Hyperresponsivity to Low-Dose Endotoxin during Progression to Nonalcoholic Steatohepatitis Is Regulated by Leptin-Mediated Signaling显示文摘 | Kento Imajo Koji Fujita Masato Yoneda Yuichi Nozaki Yuji Ogawa Yoshiyasu Shinohara Shingo Kato Hironori Mawatari Wataru Shibata Hiroshi Kitani Kenichi Ikejima Hiroyuki Kirikoshi Noriko Nakajima Satoru Saito Shiro Maeyama Sumio Watanabe Koichiro Wada Atsus | 2012 | Cell Metabolism2012，，1： | 2 |
| 17 | Submucosal hematoma 是一高度暗示的作出对 \O 有利的裁决淀粉的轻链的淀粉样变性病: 二份案例报告显示文摘 The clinical and endoscopic features of amyloid lightchain(AL) amyloidosis are diverse and mimic various other diseases.Endoscopically,few reports on submucosal hematomas of the gastrointestinal(GI) tract are available in the literature.Here,we report two cases of AL amyloidosis presenting as submucosal hematomas in the absence of clinical disease elsewhere in the body.The 2 cases were referred to our hospital because of hematochezia.The endoscopic findings in both cases were similar in submucosal hematoma formation.However,the clinical courses differed.In the first case,there was no evidence of systemic amyloidosis and the disease was conservatively managed.In the second case,the disease progressed to systemic amyloidosis and the patient died within a short time.We conclude that the endoscopic detection of a submucosal hematoma in the setting of GI bleeding should raise suspicion of AL amyloidosis.Referral to a hematologist should be done immediately for treatment while the involvement is limited to the GI tract. | Shinji Yoshii Katsuhiro Mabe Katsuhiko Nosho Hiroyuki Yamamoto Hiroshi Yasui Hiroyuki Okuda Akira Suzuki Masahiro Fujita Toshihiro Sato | 2012 | World Journal of Gastrointestinal Endoscopy2012，4，9： | 2 |
| 18 | Serum surfactant protein D is increased in acute and chronic inflammation in mice显示文摘 | Masaki Fujita John M. Shannon Hiroshi Ouchi Dennis R. Voelker Yoichi Nakanishi Robert J. Mason | 2005 | Cytokine2005，，1： | 2 |
| 19 | Comparison of Perioperative and Long-term Outcomes of Total and Proximal Gastrectomy for Early Gastric Cancer: A Multi-institutional Retrospective Study显示文摘 | Toru Masuzawa Shuji Takiguchi Motohiro Hirao Hiroshi Imamura Yutaka Kimura Junya Fujita Isao Miyashiro Shigeyuki Tamura Masahiro Hiratsuka Kenji Kobayashi Yoshiyuki Fujiwara Masaki Mori Yuichiro Doki | 2014 | World Journal of Surgery2014，，5： | 2 |
| 20 | A comparison of postoperative quality of life and dysfunction after Billroth I and Roux-en-Y reconstruction following distal gastrectomy for gastric cancer: results from a multi-institutional RCT显示文摘 | Shuji Takiguchi Kazuyoshi Yamamoto Motohiro Hirao Hiroshi Imamura Junya Fujita Masahiko Yano Kenji Kobayashi Yutaka Kimura Yukinori Kurokawa Masaki Mori Yuichiro Doki | 2012 | Gastric Cancer2012，，2： | 2 |
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