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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Cell surface antigen CD109 is a novel member of the ct 2' maeroglobulin/C3, C4, C5 family of thioester-containing proteins 显示文摘 | Lin M Sutherland D R Horsfall W | 2002 | Blood2002，99，5： | 1 |
| 3 | Effect of bioaugmentation on the performance of activated sludge reactors 显示文摘 | Hung Y T Shah D B Horsfall F L | 1987 | ProcessBiochemistry1987，，7： | 1 |
| 4 | Expression and localization of homeodomain proteins DLX/HB9 in normal and malignant human breast tissues 显示文摘 | Neufling PJ Kalionis B Horsfall D J | 2003 | Anticancer Res2003，23，： | 1 |
| 5 | Medication beliefs predict medication adherence in ambulatory patients with decompensated cirrhosis显示文摘AIM To investigate the impact of medication beliefs, illness perceptions and quality of life on medication adherence in people with decompensated cirrhosis.METHODS One hundred adults with decompensated cirrhosis completed a structured questionnaire when they attended for routine outpatient hepatology review. Measures of self-reported medication adherence(Morisky Medication Adherence Scale), beliefs surrounding medications(Beliefs about Medicines Questionnaire), perceptions of illness and medicines(Brief Illness Perception Questionnaire), and quality of life(Chronic Liver Disease Questionnaire) were examined. Clinical data were obtained via patient history and review of medical records. Least absolute shrinkage and selection operator and stepwise backwards regression techniques were used to construct the multivariable logistic regression model. Statistical significance was set at alpha = 0.05.RESULTS Medication adherence was ' High ' in 42 % o f participants, 'Medium' in 37%, and 'Low' in 21%. Compared to patients with 'High' adherence, those with 'Medium' or 'Low' adherence were more likely to report difficulty affording their medications(P < 0.001), lower perception of treatment helpfulness(P = 0.003) and stronger medication concerns relative to medication necessity beliefs(P = 0.003). People with 'Low' adherence also experienced greater symptom burden and poorer quality of life, including more frequent abdominal pain(P = 0.023), shortness of breath(P = 0.030), and emotional disturbances(P = 0.050). Multivariable analysis identified having stronger medication concerns relative to necessity beliefs(Necessity-Concerns Differential ≤ 5, OR = 3.66, 95%CI: 1.18-11.40) and more frequent shortness of breath(shortness of breath score ≤ 3, OR = 3.87,95%CI: 1.22-12.25) as independent predictors of 'Low'adherence.CONCLUSION The association between 'Low' adherence and patients having strong concerns or doubting the necessity or helpfulness of their medications should be explored further given the clinical relevance. | Kelly L Hayward Patricia C Valery Jennifer H Martin Antara Karmakar Preya J Patel Leigh U Horsfall Caroline J Tallis Katherine A Stuart Penny L Wright David D Smith Katharine M Irvine Elizabeth E Powell W Neil Cottrell | 2017 | World Journal of Gastroenterology2017，23，40： | 1 |
| 6 | Fine specificity of the autoimmune response to the Ro/SSA and La/SSB ribonucle- oproteins 显示文摘 | Scofield RH Farris D Horsfall AC | 1999 | Arthritis Rheum1999，42，： | 1 |
| 7 | Heavy metal contaminants and processing effects on the composition,storage stability and fatty acid profiles of five common commercially available fish species in Oron Local Government,Nigeria显示文摘 | Eboh L Horsfall D M Mayo B E | 2006 | Food Chemistry2006，，97： | 1 |
| 8 | Experimental transmission of pneumonia in sheep with a myeoplasm isolated from pneumonie sheep lung 显示文摘 | George T D Sullivan N D Love J A Horsfall N | 1971 | Australian Veterinary Journal1971，，47： | 1 |
| 9 | Cell surface antigen CDI09 is a novel member of the alpha2-mac- roglobulin/C3, CA, C5 family of thioester-containing proteins 显示文摘 | Lin M Sutherland D R Horsfall W | 2002 | Blood2002，99，5： | 1 |
| 10 | Sorption kinetics of Pb2 ~ and Cu2 ~ ions from aqueous solution by Nipah palm ( Nypa fruticans Wurmb) shoot biomass 显示文摘 | Wankasi D Horsfall Jr Spiff A I | 2006 | Electronic Journal Biotechnology2006，9，： | 1 |
| 11 | Relationship between ploidy and steroid hormone receptors in primary invasive breast cancer显示文摘 | Horsfall D J Tilley W D Orell S R | 1986 | Rr J Cancer1986，53，1： | 1 |
| 12 | The effect of through-thickness cracks on the ballistic performance of ceramic armour systems 显示文摘 | Horsfall I Buckley D | 1996 | Int J Impact Engng1996，18，3： | 1 |
| 13 | Cell surface antigen CD109 is a novel member of the alpha(2) macroglobulin/C 3, C 4, C 5 family of thioester-containing proteins显示文摘 | Lin M Sutherland D R Horsfall W | 2002 | Blood2002，99，5： | 1 |
| 14 | Cell surface antigen CD109is a novel member of the alpha 2macroglobulin/C3,C4,C5fami-ly of thioester-containing proteins显示文摘 | Lin M Sutherland D R Horsfall W | 2002 | Blood2002，99，5： | 1 |
| 15 | Sorption kinetics of Pb2+ and Cu2+ ions from aqueous solution byNipah palm (Nypa fruticans Wurmb) shoot biomass显示文摘 | WANKASI D HORSFALL JNR M IBUTEME SPIFF A | 2006 | Electronic Journal of Biotechnology2006，9，5： | 1 |
| 16 | Medical Tourism:A cost or benefit to the NHS?显示文摘 | Hanefeld J Horsfall D Lunt N | 2013 | Plos One2013，8，10： | 1 |
| 17 | Expression of the an- drogen receptor and an androgen-responsive protein, apolipoprotein D, in human breast cancer 显示文摘 | Hall RE Aspinall JO Horsfall D J | 1996 | Br J Cancer1996，74，8： | 1 |
| 18 | Concurrent infection with influenza A and B viruses in a single epidemic of influenza 显示文摘 | Kilbourne E D Anderson H C Horsfall F L Jr | 1951 | J Immunol1951，67，： | 1 |
| 19 | Effects of oestradiol- 17βand 5α-dihydrotestosterone on guinea-pig prostate smooth muscle cell proliferation and steroid receptor expression in vitro 显示文摘 | Ricciardelli C Horsfall D J Sykes PJ | 1994 | J Endocrin1994，140，3： | 1 |
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