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| 1 | Novel Cold-adaptive Penicillium Strain FS010 Secreting Thermo-labile Xylanase Isolated from Yellow Sea显示文摘新奇寒冷适应的 xylanolytic 毛丛紧张 FS010 从黄海沉积被孤立。海洋的真菌从 4 ~ 20 度成长了很好;一更低(0 度) 或(37 度) 更高,温度限制它的生长。紧张作为毛丛 chrysogenum 被识别。与 mesophilic P 相比。chrysogenum,寒冷适应的真菌分泌了显示出高水疗院的寒冷活跃的 xylanase (XYL ) 在低温度(2-15 度) 的 lysin 的活动和到高温度(>50 度) 的高敏感。XYL 基因从寒冷适应的 P 被孤立。chrysogenum FS010 并且指定了为 xyl。xyl 编码的蛋白质的推出的氨基酸顺序与 glycoside 水疗院的顺序显示出高相同放射激光家庭 10。基因是克隆进表示向量 pGEX-4T-1 的潜水艇,编码蛋白质完了在 Escherichia 关口 i BL21 与 glutathione-S-transferase 表示了为熔化蛋白质。表示产品被净化并且使遭到了到酶的描述。为 recombinant XYL 的最佳的温度和 pH 是 25 度并且 5.5 分别地。Recombinant XYL 在 4 度显示出将近 80% 它的最大的活动并且在 pH 范围 3.0-9.5 是活跃的。 | Yun-Hua HOU Tian-Hong WANG Hao LONG Hui-Yuan ZHU | 2006 | Acta Biochimica et Biophysica Sinica2006，38，2： | 15 |
| 2 | TCM-based new drug discovery and development in China显示文摘Over the past 30 years, China has significantly improved the drug development environment by establishing a series of policies for the regulation of new drug approval. The regulatory system for new drug evaluation and registration in China was gradually developed in accordance with international standards. The approval and registration of TCM in China became as strict as those of chemical drugs and biological products. In this review, TCM-based new drug discovery and development are introduced according to the TCM classification of nine categories. | WU Wan-Ying HOU Jin-Jun LONG Hua-Li YANG Wen-Zhi LIANG Jian GUO De-An | 2014 | Chinese Journal of Natural Medicines2014，12，4： | 15 |
| 3 | Laparoscopic hepaticoplasty using gallbladder as a subcutaneous tunnel for hepatolithiasis显示文摘AIM:To investigate the feasibility,efficacy and safety of laparoscopic hepaticoplasty using gallbladder as subcutaneous tunnel and sphincter-of-Oddi preservation for hepatolithiasis.METHODS:From January 2010 to July 2013,six patients with hepatolithiasis were treated at our institution.All the patients underwent laparoscopic surgery.The procedures included common hepatic duct exploration,stone clearance by fiberoptic choledochoscopy,hilar bile duct hepaticoplasty with preservation of the sphincter of Oddi,anastomosis between the hilar bile duct and neck of the gallbladder,and establishment of a subcutaneous tunnel with the gallbladder.Two patients underwent left lateral hepatectomy simultaneously.Clinical data including operation time,intraoperative blood loss,operative morbidity,hospital mortality,stone clearance,and recurrence rate were analyzed.RESULTS:All patients successfully completed laparoscopic surgery.The mean length of hospital stay was4.5±0.9 d(range:3-6 d).The mean blood loss of the hepatectomy was 450 mL(range:200-700 mL),and the blood loss of the other four was 137±151 mL (range:50-400 mL).The mean operative time was 318±68 min(range:236-450 min).The operative morbidity and hospital mortality were zero.The immediate stone clearance rate was 100%.All patients were followed up for an average of 17 mo(range:7-36 mo).One of the six patients had abdominal mass with pain,and subcutaneous tunnel cholangiography showed severe gallbladder-biliary anastomotic stricture at 4 mo postoperatively.There was no stone recurrence and no cholangitis during follow-up.CONCLUSION:Laparoscopic hepaticoplasty using gallbladder with a subcutaneous tunnel and preserving the sphincter of Oddi is feasible,safe and effective for hepatholithiasis. | Long Cui Zhi Xu Xiao-Feng Ling Li-Xin Wang Chun-Sheng Hou Gang Wang Xiao-Si Zhou | 2014 | World Journal of Gastroenterology2014，20，12： | 13 |
| 4 | Realizing over 10% efficiency in polymer solar cell by device optimization显示文摘The low band gap polymer based on benzodithiophene(BDT)-thieno[3,4-b]thiophene(TT)backbone,PBDT-TS1,was synthesized following our previous work and the bulk heterojunction(BHJ)material comprising PBDT-TS1/PC71BM was optimized and characterized.By processing the active layer with different additives i.e.1,8-diiodooctane(DIO),1-chloronaphthalene(CN)and 1,8-octanedithiol(ODT)and optimizing the ratio of each additive in the host solvent,a high PCE of 9.98%was obtained under the condition of utilizing 3%DIO as processing additive in CB.The effect of varied additives on photovoltaic performance was illustrated with atomic force microscopy(AFM)and transmission electron microscope(TEM)measurements that explained changes in photovoltaic parameters.These results provide valuable information of solvent additive choice in device optimization of PBDTTT polymers,and the systematic device optimization could be applied in other efficient photovoltaic polymers.Apparently,this work presents a great advance in single junction PSCs,especially in PSCs with conventional architecture. | Shaoqing Zhang Long Ye Wenchao Zhao Bei Yang Qi Wang Jianhui Hou | 2015 | Science China Chemistry2015，58，2： | 12 |
| 5 | Major advances in studies of the physical geography and living environment of China during the past 70 years and future prospects显示文摘The natural environment provides material essentials for human survival and development. The characteristics,processes, regional differentiation and forcing mechanisms of the elements of the natural environment(e.g. geomorphology,climate, hydrology, soil, etc.) are the main objects of research in physical geography. China has a complex natural environment and huge regional differentiation and therefore it provides outstanding reserach opportunities in physical geography. This review summarizes the most important developments and the main contributions of research in the physical geography and human living environment in China during the past 70 years. The major topics addressed are the uplift of the Tibetan Plateau and the evolution of its cryosphere, the development of fluvial systems, the acidification of the vast arid region of the Asian interior, variations in the monsoon and westerly climate systems on multiple timescales, the development of lakes and wetlands, the watershed system model, soil erosion, past human-environment interactions, biogeography, and physical geographic zonality. After briefly introducing international research developments, we review the history of research in physical geography in China, focusing on the major achievements and major academic debates, and finally we summarize the status of current research and the future prospects. We propose that in the context of the national demand for the construction of an ecological civilization, we should make full use of the research findings of physical geography, and determine the patterns and mechanisms of natural environmental processes in order to continue to promote the continued contribution of physical geography to national development strategies, and to further contribute to the theory of physical geography from a global perspective. | Fahu CHEN Bojie FU Jun XIA Duo WU Shaohong WU Yili ZHANG Hang SUN Yu LIU Xiaomin FANG Boqiang QIN Xin LI Tingjun ZHANG Baoyuan LIU Zhibao DONG Shugui HOU Lide TIAN Baiqing XU Guanghui DONG Jingyun ZHENG Wei YANG Xin WANG Zaijun LI Fei Wang Zhenbo HU Jie WANG Jianbao LIU Jianhui CHEN Wei HUANG Juzhi HOU Qiufang CAI Hao LONG Ming JIANG Yaxian HU Xiaoming FENG Xingguo MO Xiaoyan YANG Dongju ZHANG Xiuhong WANG Yunhe YIN Xiaochen LIU | 2019 | Science China Earth Sciences2019，62，11： | 11 |
| 6 | Space experimental studies of microgravity fluid science in China显示文摘Microgravity fluid physics is an important part of microgravity sciences, which consists of simple fluids of many new systems, gas-liquid two-phase flow and heat transfer, and complex fluid mechanics. In addition to the importance of itself in sciences and applications, microgravity fluid physics closely relates to microgravity combustion, space biotechnology and space materials science, and promotes the developments of interdisciplinary fields. Many space microgravity experiments have been performed on board the recoverable satellites and space ships of China and pushed the rapid development of microgravity sciences in China. In the present paper, space experimental studies and the main results of the microgravity fluid science in China in the last 10 years or so are introduced briefly. | HU WenRui LONG Mian KANG Qi XIE JingChang HOU MeiYing ZHAO JianFu DUAN Li WANG ShuangFeng | 2009 | Chinese Science Bulletin2009，54，22： | 10 |
| 7 | A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways显示文摘Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD < 3%), precise(RSD 0.63%-2.05%), and linear(R > 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root). | ZHANG Yi-Bei DA Juan ZHANG Jing-Xian LI Shang-Rong CHEN Xin LONG Hua-Li WANG Qiu-Rong CAI Lu-Ying YAO Shuai HOU Jin-Jun WU Wan-Ying GUO De-An | 2017 | Chinese Journal of Natural Medicines2017，15，4： | 10 |
| 8 | Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients显示文摘AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome(IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients. METHODS: Visceral hypersensitivity was developed using the maternal separation(MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16 S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort.RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and postinflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls. CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research. | Xiao-Yan Zhou Ming Li Xia Li Xin Long Xiu-Li Zuo Xiao-Hua Hou Ying-Zi Cong Yan-Qing Li | 2016 | World Journal of Gastroenterology2016，22，22： | 10 |
| 9 | The long rather than the short allele of 5-HTTLPR predisposes Han Chinese to anxiety and reduced connectivity between prefrontal cortex and amygdala显示文摘The short allele of the serotonin-transporter gene is associated with higher risk for anxiety and depression in Caucasians, but this association is still unclear in Asians. Here, we addressed this issue using behavioral and multi-modal MRI approaches in a large group of healthy Han Chinese participants (n = 233). In contrast to findings in Caucasians, we found that long-allele (L) carriers had higher anxiety scores. In another group (n = 64) experiencing significant levels of depression or anxiety, the L-allele frequency was also significantly higher. In healthy participants, L-carriers had reduced functional and anatomical connectivity between the amygdala and prefrontal cortex (PFC), which was correlated with anxiety or depression scores. Our findings demonstrated that in Chinese Han participants, in contrast to Caucasians, the L-allele confers vulnerability to anxiety or depression and weakens top-down emotional control between the PFC and amygdala. Therefore, ethnic background should be taken into account in gene-related studies and their potential clinical applications. | Haixia Long Bing Liu Bing Hou Chao Wang Jin Li Wen Qin Dawei Wang Yuan Zhou Keith M. Kendrick Chunshui Yu Tianzi Jiang | 2013 | Neuroscience Bulletin2013，29，1： | 9 |
| 10 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 11 | Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation显示文摘有长期的 prostatitis/chronic 的人的表示 prostatic 分泌物(EPS ) 骨盆的疼痛症候群(CP/CPPS ) ,不肥沃的人和正常的人受到 microbiological 学习。EPS 从这些题目被收集,它包括了 26 个正常的人, 11 个不肥沃的病人和 51 个 CP/CPPS 病人。DNA 从每个标本被提取,并且 16S rRNA 基因的 V3 区域用通用细菌的教材被放大。结果证明在 CP/CPPS 和不肥沃的病人的基因积极的率多是的 EPS 16S rRNA 比在正常的人高,但是没有在三个耐心的组之中的任何差别。使中毒的坡度胶化电气泳动(DGGE ) 方法被用来描绘从有 CP/CPPS 或不孕的病人的前列腺液体的细菌的社区结构发出的 EPS。主要部件分析(PCA ) 和部分最少的广场(请) PCR-DGGE 侧面的分析表明 EPS 细菌的社区结构在三个组之中不同。三个乐队作为为在 CP/CPPS 病人和不肥沃的病人之间的差异负责的关键因素被识别(P< 0.05 ) 。二个乐队在范畴 IIIA 和范畴 IIIB 前列腺炎病人的差异作为优先级因素被识别(P< 0.05 ) 。根据这研究,当看作了微型环境时,前列腺和低尿道道的生态的平衡可能在一条健康的男繁殖的道的维护起一个重要作用。 | Dong-Sheng Hou Wen-Min Long Jian Shen Li-Ping Zhao Xiao-Yan Pang Chen Xu | 2012 | Asian Journal of Andrology2012，14，4： | 8 |
| 12 | Blocking of N-acetylglucosaminyltransferase V induces cellular endoplasmic reticulum stress in human hepatocarcinoma 7721 cells显示文摘N-acetylglucosaminyltransferase V (GnT-V ) 是重要 tumorigenesis 和联系转移的酶。为了学习它的简历,工作, GnT-V 稳定地压制了房间线(GnT-V-AS/7,721 ) 在以前的学习从 7,721 个 hepatocarcinoma 房间被构造。在这研究, cDNA 数组基因表示侧面在 GnT-V-AS/7,721 之间被比较并且父母 7,721 个房间。数据显示 GnT-V-AS/7,721 显示出与 ER 压力一致的一个典型表达式模式。ER 压力的分子的机制被分析在二展开的蛋白质回答(UPR ) 在关键分子上在 GnT-V-AS/7,721 探索小径。为 ATF6 和 Ire1/XBP-1 小径,它被 BIP 的起来规定在 mRNA 和蛋白质证实 XBP-1 的拼接的形式的水平,和外观。至于 PERK/eIF2alpha 小径,激活嗯 eIF2alpha kinase 津贴被观察。从 GnT-V-AS/7,721 房间证实结果,在 UPR 的关键分子在 7,721 个房间再被检验由特定的 RNAi 处理防碍 GnT-V。结果与从 GnT-V-AS/7721 的那些是类似的,显示那块 GnT-V 能明确地激活嗯在 7,721 个房间的应力。率(3 )H 人加入与率改正了(3 ) 在 GnT-V-AS/7,721 的 H 列伊加入与控制相比极大地是下面调整的,它表明了综合的酶的缺乏的功能在堵住的 GnT-V 以后的 N-glycans。而且,与 underglycosylation 联系的女伴 GRP94 的更快的移居形式,和细胞内部的 glycoproteins 的广泛地改变的 N-glycans 结构是也在 GnT-V-AS/7,721 检测了。这些结果支持了机制损害的那块 GnT-V 表达式女伴和 N-glycan-synthesizing 酶工作,它在 vivo 引起了 UPR。 | Huan Fang Wei Huang Ying Ying Xu Zong Hou Shen Chao Qun Wu Shou Yi Qiao Yan Xu Long Yu Hui Li Chen | 2006 | Cell Research2006，16，1： | 7 |
| 13 | Validation and application of water levels derived from Sentinel-3A for the Brahmaputra River显示文摘For the first time ever, this study aims at applying Sentinel-3 A to the Great Brahmaputra River(GBR) and validating water levels derived from this newly-launched altimetry satellite mission. The GBR is divided into three primary parts:(1) a large section of the Yarlung Zangbo River in China, also termed the Upper Brahmaputra River(UBR) in this study, featured by high elevation,complex terrain, narrow river widths(from less than 100 to 400 m), and limited in situ measurements;(2) the Middle Brahmaputra River(MBR) with widths varying from ~400 m to ~1 km;(3) the Lower Brahmaputra River(LBR), dominated by braided channels with wide river channels(up to several kilometers). For the altimetry data, both waveform retracking and hooking effect correction were applied to mitigate the influence caused by land contamination and to improve the accuracy of water level retrievals. Water levels were derived from 41 virtual stations(VSs) across the GBR and different retracking algorithms were compared with in situ data from two gauging stations in the UBR. Time series of altimetry-based water levels were categorized into three types based on the quality: no data(type 1), degraded(type 2), and good(type 3). Results showed that the VSs(type 1) only existed in the mountainous regions, accounting for ~ a half of the total in the UBR. Validation with the gauged water levels showed that the TIC algorithm performed best among all of the retrackers applied, followed by the Ice-1 algorithm. The standard deviation of the difference between the gauged and TIC-derived water levels ranged from 0.41 to 0.76 m among four different VSs(type 3). In addition, the quality of VSs in the LBR was best, followed by the MBR. Our study has demonstrated the capability of Sentinel-3 A in monitoring water levels in the GBR, thereby paving the way for future applications such as discharge estimation and hydrologic/hydrodynamic applications. | HUANG Qi LI XingDong HAN PengFei LONG Di ZHAO FanYu HOU AiZhong | 2019 | Science China(Technological Sciences)2019，62，10： | 6 |
| 14 | An improved modeling of precipitation phase and snow in the Lancang River Basin in Southwest China显示文摘Precipitation phase(e.g., rainfall and snowfall) and snow(e.g., snowpack and snowmelt runoff) in high-mountain regions may largely affect runoff generation, which is critical to water supply, hydropower generation, agricultural irrigation, and ecosystems downstream. Accurately modeling precipitation phase and snow is therefore fundamental to developing a better understanding of hydrological processes for high-mountain regions and their lower reaches. The Lancang River(LR, or the Upper Mekong River)in China, among the most important transboundary rivers originating from the Tibetan Plateau, features active dam construction and complex water resources allocation of various stakeholders in Southeast Asian countries under climate change. This study aims to improve precipitation phase and snow modeling for the LR basin with a hydrological model and multisource remotely sensed data. Results show that joint use of the Moderate Resolution Imaging Spectroradiometer(MODIS) land surface temperature product with high spatial resolution(1 km×1 km) and an air temperature product can more precisely distinguish precipitation phase than air and wet-bulb temperature products in the LR basin. Snowfall and snowmelt were found to be controlled primarily by rainfall and snowfall temperature thresholds in snow modeling. The rainfall and snowfall temperature thresholds derived from the hydrological model through calibration with remotely sensed snowpack at basin scales were considerably lower than those derived from in situ observations. Rainfall and snowfall temperature thresholds derived from in situ observations could lead to the overestimation of snowmelt runoff due mostly to the lack of representation of point-based measurements at basin scales. This study serves as a basis for better modeling and predicting snow for the LR basin and potentially other similar basins globally. | HAN ZhongYing LONG Di HAN PengFei HUANG Qi DU MingDa HOU AiZhong | 2021 | Science China(Technological Sciences)2021，64，7： | 6 |
| 15 | Effect of tin addition on corrosion behavior of a low-alloy steel in simulated costal-industrial atmosphere显示文摘The effect of tin addition on the atmospheric corrosion behavior of a low-alloy steel in sim ulated coastalindustrial atm osphere has been investigated by indoor wet/dry cyclic corrosion test (CCT). The results indicate that tin addition can obviously make the steel substrate more resistant to atmospheric corrosion by suppressing the cathodic H^+ reduction reaction, and but tin addition is not of obvious beneficial effect when the steel is covered with a thicker rust layer during long-term corrosion process. The reason lies in the fact that the presence of un-reduced H^+ can lower the electrolyte pH value and lead to a loose and porous rust layer on tin-containing steel sample than that on tin-free steel sample. In addition, the 120 CCT cycles corrosion process of the two steels can be divided into three stages. Both the tin-free and tin-containing steels show an increasing corrosion rate during the initial corrosion stage and then exhibit a decreasing corrosion rate during the second and third corrosion stages. Moreover, tin addition makes the tin-containing steel rust layer have a higher amount of α-FeOOH and lower am ount of γ-FeOOH and Fe3O4 than the tin-free steel rust layer. | Bo Liu Xin Mu Ying Yang Long Hao Xueyong Ding Junhua Dong Zhe Zhang Huaxing Hou Wei Ke | 2019 | Journal of Materials Science & Technology2019，35，7： | 6 |
| 16 | Oddi sphincter preserved cholangioplasty with hepaticosubcutaneous stoma for hepatolithiasis显示文摘AIM: To evaluate the long-term outcomes of Oddi sphincter preserved cholangioplasty with hepaticosubcutaneous stoma(OSPCHS) and risk factors for recurrence in hepatolithiasis.METHODS: From March 1993 to December 2012, 202 consecutive patients with hepatolithiasis underwent OSPCHS at our department. The Oddi sphincter preserved procedure consisted of common hepatic duct exploration, stone extraction, hilar bile duct plasty, establishment of subcutaneous stoma to the bile duct. Patients with recurrent stones can undergo stone extraction and/or biliary drainage via the subcutaneous stoma which can be incised under local anesthesia. The long-term results were reviewed. Cox regression model was employed to analyze the risk factors for stone recurrence.RESULTS: Ninety-seven(48.0%) OSPCHS patients underwent hepatic resection concomitantly. The rate of surgical complications was 10.4%. There was no perioperative death. The immediate stone clearance rate was 72.8%. Postoperative cholangioscopic lithotomy raised the clearance rate to 97.0%. With a median follow-up period of 78.5 mo(range: 2-233 mo), 24.8% of patients had recurrent stones, 2.5% had late development of cholangiocarcinoma, and the mortality rate was 5.4%. Removal of recurrent stones and/or drainage of inflammatory bile via subcutaneous stoma were conducted in 44(21.8%) patients. The clearance rate of recurrent stones was 84.0% after subsequent choledochoscopic lithotripsy via subcutaneous stoma.Cox regression analysis showed that residual stone was an independent prognostic factor for stone recurrence.CONCLUSION: In selected patients with hepatolithiasis, OSPCHS achieves excellent long-term outcomes, and residual stone is an independent prognostic factor for stone recurrence. | Yu-Gui Lian Wei-Tao Zhang Zhi Xu Xiao-Feng Ling Li-Xin Wang Chun-Sheng Hou Gang Wang Long Cui Xiao-Si Zhou | 2015 | World Journal of Gastroenterology2015，21，45： | 6 |
| 17 | Cloning,Sequencing and Expression Analysis of the First Cellulase Gene Encoding Cellobiohydrolase 1 from a Cold-adaptive Penicillium chrysogenum FS010显示文摘cellobiohydrolase 1 基因(cbh1 ) 从毛丛 chrysogenumFS010 被克隆由一修改了 cbhl 有开的读的 .DNAsequencing 表演 1590 bp 装裱的热不对称的交叉聚合酶链反应(TAIL-PCR ) ,编码 529amino 酸残余的通常认为的蛋白质。推出的氨基酸顺序表明 CBHI 与 56 kDa 的一个预言的分子的团有模块化的结构并且由 threonine 与催化的域分开的一个真菌的类型糖类绑定模块组成富有的连接器区域。通常认为的基因产品对在家庭 7 glycosyl hydrolases.A 小说 cbh1 倡导者( 1.3 kb )的真菌的 cellobiohydrolases 相应也被克隆并且定序,它为各种各样的碳来源的碳新陈代谢作用之副产物抑压者 CRE1.Effect 包含七个通常认为的结合部位(5'-SYGGRG-3')到 P.chrysogenum 的 cbh1 抄写被北分析检验,建议 cbh1 的表达式在 transcriptional 被调整水平。在寒冷适应的真菌 P.chysogenum 的 cbh1 基因 accumulatedintracellularly 在 Saccharomyces cerevisiae H158.The recombinant CBHI 被表示为活跃的酶并且不能被分泌进媒介。 | Yunhua HOU~(1,2) Tianhong WANG~1 Hao LONG~1 and Huiynan ZHU~(1,2) ~1State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China ~2Department of Food and Biologic Engineering,Shandong Institute of Light Industry,Jinan 250323,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 6 |
| 18 | Robot assisted rehabilitation of the arm after stroke:prototype design and clinical evaluation显示文摘Robot assisted rehabilitation training is a promising tool for post-stroke patients' recovery, and some new challenges are imposed on robot design, control, and clinical evaluation. This paper presents a novel upper limb rehabilitation robot that can provide safe and compliant force feedbacks to the patient for the benefits of its stiff and low-inertia parallel structure, highly backdrivable capstan-cable transmission, and impedance control method in the workspace. The 'assist-as-needed'(AAN) clinical training principle is implemented through the 'virtual tunnel' force field design, the 'assistance threshold' strategy, as well as the virtual environment training games, and preliminary clinical results show its effectiveness for motor relearning for both acute and chronic stroke patients, especially for coordinated movements of shoulder and elbow. | Liang PENG Zeng-Guang HOU Long PENG Lincong LUO Weiqun WANG | 2017 | Science China(Information Sciences)2017，60，7： | 6 |
| 19 | Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro显示文摘 | Jin-ming LI Wei CHEN Hao WANG Chen JIN Xian-jun YU Wei-yue LU Long CUI De-liang FU Quan-xing NI Hui-min HOU | 2009 | Acta Pharmacologica Sinica2009，30，9： | 5 |
| 20 | Anti-proliferation activity of terpenoids isolated from Euphorbia kansui in human cancer cells and their structure-activity relationship显示文摘Euphorbia kansui is a commonly used traditional Chinese medicine for the treatment of edema,pleural effusion,and asthma,etc.According to the previous researches,terpenoids in E.kansui possess various biological activities,e.g.,anti-virus,anti-allergy,antitumor effects.In this work,twenty five terpenoids were isolated from E.kansui,including thirteen ingenane-and eight jatrophane-type diterpenoids(with two new compounds,kansuinin P and Q)and four triterpenoids.Eighteen of them were analyzed by MTS assay for in vitro anticancer activity in five human cancer cell lines.Structure-activity relationship for 12 ingenane-type diterpenoids in colorectal cancer Colo205 cells were preliminary studied.Significant anti-proliferation activities were observed in human melanoma cells breast cancer MDA-MB-435 cells and Colo205 cells.More than half of the isolated ingenane-type diterpenoids showed inhibitory activities in MDA-MB-435 cells.Eight ingenane-and one jatrophane-type diterpenoids possessed much lower IC_(50) values in MDA-MB-435 cells than positive control staurosporine.Preliminary structure-activity relationship analysis showed that substituent on position 20 was important for the activity of ingenane-type diterpenoids in Colo205 cells and substituent on position 3 contributed more significant biological activity of the compounds than that on position 5 in both MDA-MB-435 and Colo205 cells. | HOU Jin-Jun SHEN Yao YANG Zhou FANG Lin CAI Lu-Ying YAO Shuai LONG Hua-Li WU Wan-Ying GUO De-An | 2017 | Chinese Journal of Natural Medicines2017，15，10： | 5 |
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