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| 1 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 2 | Collagen-binding basic fibroblast growth factor improves functional remodeling of scarred endometrium in uterine infertile women: a pilot study显示文摘Intrauterine adhesion(IUA) is a common cause of uterine infertility and one of the most severe clinical features is endometrial fibrosis namely endometrial scarring for which there are few cures currently. Blocked angiogenesis is the main pathological change in the scarred endometrium. The fibroblast growth factor 2(b FGF), a member of FGF family, is usually applied to promote healing of refractory ulcer and contributes to angiogenesis of tissues. In this study, the sustained-release system of b FGF100 μg was administrated around scarred endometrium guiding by ultrasound every 4 weeks in 18 patients(2–4 times). Results showed that after treatment, the menstrual blood volume, endometrial thickness and the scarred endometrial area were improved.Histological study showed blood vessel density increased obviously. Three patients(3/18) achieved pregnancy over 20 gestational weeks. Therefore, administrating the b FGF surrounding scarred endometrium may provide a new therapeutic approach for the patients with endometrial fibrosis. | Peipei Jiang Xiaoqiu Tang Huiyan Wang Chenyan Dai Jing Su Hui Zhu Minmin Song Jingyu Liu Ziqing Nan Tong Ru Yaling Li Jingmei Wang Jun Yang Bing Chen Jianwu Dai Yali Hu | 2019 | Science China(Life Sciences)2019，62，12： | 16 |
| 3 | Mutation profile and its correlation with clinicopathology in Chinese hepatocellular carcinoma patients显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most common causes of cancer worldwide.Although many studies have focused on oncogene characteristics,the genomic landscape of Chinese HCC patients has not been fully clarified.Methods:A total of 165 HCC patients,including 146 males and 19 females,were enrolled.The median age was 55 years(range,27-78 years).Corresponding clinical and pathological information was collected for further analysis.A total of 168 tumor tissues from these patients were selected for next-generation sequencing(NGS)-based 450 panel gene sequencing.Genomic alterations including single nucleotide variations(SNV),short and long insertions and deletions(InDels),copy number variations,and gene rearrangements were analyzed.Tumor mutational burden(TMB)was measured by an algorithm developed in-house.The top quartile of HCC was classified as TMB high.Results:A total of 1,004 genomic alterations were detected from 258 genes in 168 HCC tissues.TMB values were identified in 160 HCC specimens,with a median TMB of 5.4 Muts/Mb(range,0-28.4 Muts/Mb)and a 75%TMB of 7.7 Muts/Mb.The most commonly mutated genes were TP53,TERT,CTNNB1,AXIN1,RB1,TSC2,CCND1,ARID1A,and FGF19.SNV was the most common mutation type and C:G>T:A and guanine transformation were the most common SNVs.Compared to wild-type patients,the proportion of Edmondson grade III-IV and microvascular invasion was significantly higher in TP53 mutated patients(P<0.05).The proportion of tumors invading the hepatic capsule was significantly higher in TERT mutated patients(P<0.05).The proportion of Edmondson grade I-II,alpha fetoprotein(AFP)<25μmg/L,and those without a history of hepatitis B was significantly higher in CTNNB1 mutated patients(P<0.05).CTNNB1 mutations were associated with TMB high in HCC patients(P<0.05).Based on correlation analysis,the mutation of TP53 was independently correlated with microvascular invasion(P=0.002,OR=3.096)and Edmondson grade III-IV(P=0.008,OR=2.613).The mutation of TERT was independently correlated with tumor invasion of the liver capsule(P=0.001,OR=3.030),and the mutation of CTNNB1 was independently correlated with AFP(<25μmg/L)(P=0.009,OR=3.414).Conclusions:The most frequently mutated genes of HCC patients in China were TP53,TERT,and CTNNB1,which mainly lead to the occurrence and development of HCC by regulating the P53 pathway,Wnt pathway,and telomere repair pathway.There were more patients with microvascular invasion and Edmondson III-IV grade in TP53 mutated patients and more patients with hepatic capsule invasion in TERT mutated patients,while in CTNNB1 mutated patients,there were more patients with Edmondson I-II grade,AFP<25μmg/L,and a non-hepatitis B background.Also,the TMB values were significantly higher in CTNNB1 mutated patients than in wild type patients. | Shuo Wang Huasheng Shi Tao Liu Manjiang Li Sanshun Zhou Xuan Qiu Zusen Wang Weiyu Hu Weidong Guo Xiaoqian Chen Honglin Guo Xiaoliang Shi Junping Shi Yunjin Zang Jingyu Cao Liqun Wu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，2： | 8 |
| 4 | PIP5k1β controls bone homeostasis through modulating both osteoclast and osteoblast differentiation显示文摘PIP5k1βis crucial to the generation of phosphotidylinosotol(4,5)P2.PIP5k1βparticipates in numerous cellular activities,such as B cell and platelet activation,cell phagocytosis and endocytosis,cell apoptosis,and cytoskeletal organization.In the present work,we aimed to examine the function of PIP5k1βin osteoclastogenesis and osteogenesis to provide promising strategies for osteoporosis prevention and treatment.We discovered that PIP5k1β deletion in mice resulted in obvious bone loss and that PIP5k1β was highly expressed during both osteoclast and osteoblast differentiation.Deletion of the gene was found to enhance the proliferation and migration of bone marrow-derived macrophage-like cells to promote osteoclast differentiation.PIP5k1β-/-osteoclasts exhibited normal cytoskeleton architecture but stronger resorption activity.PIP5kip deficiency also promoted activation of mitogen-activated kinase and Akt signaling,enhanced TRAF6 and c-Fos expression,facilitated the expression and nuclear translocation of NFATC1,and upregulated Grb2 expression,thereby accelerating osteoclast differentiation and function.Finally,PIP5k1β enhanced osteoblast differentiation by upregulating master gene expression through triggering smad1/5/8 signaling.Therefore,PIP5k1βmodulates bone homeostasis and remodeling. | Xiaoying Zhao Penglei Cui Guoli Hu Chuandong Wang Lei Jiang Jingyu Zhao Jiake Xu Xiaoling Zhang | 2020 | Journal of Molecular Cell Biology2020，12，1： | 4 |
| 5 | Gap-free genome assembly and comparative analysis reveal the evolution and anthocyanin accumulation mechanism of Rhodomyrtus tomentosa显示文摘Rhodomyrtus tomentosa is an important f leshy-fruited tree and a well-known medicinal plant of the Myrtaceae family that is widely cultivated in tropical and subtropical areas of the world.However,studies on the evolution and genomic breeding of R.tomentosa were hindered by the lack of a reference genome.Here,we presented a chromosome-level gap-free T2T genome assembly of R.tomentosa using PacBio and ONT long read sequencing.We assembled the genome with size of 470.35 Mb and contig N50 of∼43.80 Mb with 11 pseudochromosomes.A total of 33382 genes and 239.31 Mb of repetitive sequences were annotated in this genome.Phylogenetic analysis elucidated the independent evolution of R.tomentosa starting from 14.37MYA and shared a recent WGD event with other Myrtaceae species.We identified four major compounds of anthocyanins and their synthetic pathways in R.tomentosa.Comparative genomic and gene expression analysis suggested the coloring and high anthocyanin accumulation in R.tomentosa tends to be determined by the activation of anthocyanin synthesis pathway.The positive selection and up-regulation of MYB transcription factors were the implicit factors in this process.The copy number increase of downstream anthocyanin transport-related OMT and GST gene were also detected in R.tomentosa.Expression analysis and pathway identification enriched the importance of starch degradation,response to stimuli,effect of hormones,and cell wall metabolism during the f leshy fruit development in Myrtaceae.Our genome assembly provided a foundation for investigating the origins and differentiation of Myrtaceae species and accelerated the genetic improvement of R.tomentosa. | angping Li Shiqiang Xu Zitong Xiao Jingming Wang Yu Mei Haifei Hu Jingyu Li Jieying Liu Zhuangwei Hou Junliang Zhao Shaohai Yang Jihua Wang | 2023 | Horticulture Research2023，10，3： | 3 |
| 6 | Integrative Analysis Reveals Enhanced Regulatory Effects of Human Long Intergenic Non-Coding RNAs in Lung Adenocarcinoma显示文摘Although there is an accumulating appreciation of the key roles that long intergenic non-coding RNAs(linc RNAs) play in diverse cellular processes, our knowledge of how linc RNAs function in cancer remains sparse. Here, we present a comprehensive landscape of RNA-seq transcriptome profiles of lung adenocarcinomas and their paired normal counterparts to unravel gene regulation rules of linc RNAs.Consistent with previous findings of co-expression between neighboring protein-coding genes, linc RNAs were typically co-expressed with their neighboring genes, which was found in both cancerous and normal tissues. By building a mathematical model based on correlated gene expression, we distinguished an additional subset of linc RNAs termed 'regulatory linc RNAs', representing their dominant roles in gene regulation. The number of regulatory linc RNAs was significantly higher in cancerous compared to normal tissues, and most of them positively regulated protein-coding genes in trans. Functional validation, using knockdown, determined that regulatory linc RNA,GAS5, affected its predicted protein-coding targets. Moreover, we discovered hundreds of differentially expressed regulatory linc RNAs with inclusion of some cancer-associated linc RNAs. Our integrated analysis reveals enhanced regulatory effects of linc RNAs and provides a resource for the study of regulatory linc RNAs that play critical roles in lung adenocarcinoma. | You Zhou Kai Wu Jianping Jiang Jinfei Huang Peiwei Zhang Yufei Zhu Guohong Hu Jingyu Lang Yufang Shi Landian Hu Tao Huang Xiangyin Kong | 2015 | Journal of Genetics and Genomics2015，42，8： | 3 |
| 7 | Novel ACTG1 mutation causing autosomal dominant non-syndromic hearing impairment in a Chinese family显示文摘The γ-actin (ACTG1) gene is a cytoplasmic nonmuscle actin gene, which encodes a major cytoskeletal protein in the sensory hair cells of the cochlea. Mutations in ACTG1 were found to cause autosomal dominant, progressive, sensorineural hearing loss linked to the DFNA 20/26 locus on chromosome 17q25.3 in European and American families, respectively. In this study, a novel missense mutation (c.364A>G; p.I122V) co-segregated with the affected individuals in the family and did not exist in the unaffected family members and 150 unrelated normal controls. The alteration of residue Ile122 was predicted to damage its interaction with actin-binding proteins, which may cause disruption of hair cell organization and function. These findings strongly suggested that the I122V mutation in ACTG1 caused autosomal dominant non-syndromic hearing impairment in a Chinese family and expanded the spectrum of ACTG1 mutations causing hearing loss. | Ping Liu Hu Li Xiang Ren Haiyan Mao Qihui Zhu Zhengfeng Zhu Rong Yang Wenlin Yuan Jingyu Liu Qing Wang Mugen Liu | 2008 | Journal of Genetics and Genomics2008，35，9： | 3 |
| 8 | Fluoro-Hydroxylation of gem-Difluoroalkenes:Synthesis of 18O-labeled α-CF3 Alcohols显示文摘in this paper,a fluoro-hydroxylation of gem-difluoroalkenes is demonstrated.This protocol uses sequential electrophilic fluorination with Selectfluor and nucleophilic hydroxylation with H218o,which can be used to prepare 18Oo-labeled α-CF3 alcohols from gem-difluorostyrenes.The reaction is typically carried out at room temperature within 4h in good to excellent yields.Other nucleophiles (besides H218o),such as alcohols,carboxylic acid, acetonitrile and N,N-dimethylformamide,are also suitable for this difunctionalization of gem-difluoroalkenes. | Jingyu Hu Yide Yang Zhengzhao Lou Chuanfa Ni Jinbo Hu | 2018 | Chinese Journal of Chemistry2018，36，12： | 3 |
| 9 | Mechanism and effects of snow accumulations and controls by lightweight snow fences显示文摘This paper investigates the snowdrifts caused by lightweight fences along the lines on the flatland through the computational fluid dynamics method. The characteristic ambient flows around the solid fences and the porous fences with varied heights and bottom wind gaps are simulated in the numerical model, and the working mechanism of ‘‘interception'' and ‘‘scouring'' of the lightweight fences are analyzed. Based on the friction velocities near the ground, two sets of criteria are proposed to evaluate the deposition and erosion effects of different fences. According to flow separation and reattachment, the simplified relationships between the most likely positions for snow accumulations and fence parameters are developed. The study indicates that the capabilities for snow interception by the solid fence without wind gap and the distance from which to the second snow coverage center both increase with the fence height. Furthermore, it is found that the scouring range for snow surface increases significantly with the size of wind gap, and the snow accumulation rate on the leeward side decreases with the increasing fence porosity. | Duote Liu Yongle Li Bin Wang Peng Hu Jingyu Zhang | 2016 | Journal of Modern Transportation2016，24，4： | 2 |
| 10 | Characterization of DNA damage response deficiency in pancreatic cancer patients from China显示文摘To the editor,Pancreatic ductal adenocarcinoma(PDAC)has the worst prognosis among all common malignant solid tumors,with a 5-year overall survival(OS)rate of less than 10%[1].Few effective targets for anticancer ther-apy have been confirmed in pancreatic cancer.Recently,it was substantiated that pancreatic cancer patients carry-ing deleterious mutations of the DNA damage response(DDR)genes are more likely to benefit from platinum-based chemotherapy[2]and poly(adenosine diphosphate-ribose)polymerase(PARP)inhibitor[3]. | Xiaofei Zhang Tiebo Mao Bei Zhang Haiyan Xu Jiujie Cui Feng Jiao Dongqin Chen Yu Wang Jiong Hu Qing Xia Shumin Li Ming Yue Jingyu Ma Jiayu Yao Yongchao Wang Xiao Zhang Shiqing Chen Yuezong Bai Yuexiang Wang Xuebin Zhang Qiang Liu Yongwei Sun Deliang Fu Yingbin Liu Lei Xiong Liwei Wang | 2022 | Cancer Communications2022，42，1： | 2 |
| 11 | Optimization of a precise integration method for seismic modeling based on graphic processing unit显示文摘General purpose graphic processing unit(GPU) calculation technology is gradually widely used in various fields.Its mode of single instruction,multiple threads is capable of seismic numerical simulation which has a huge quantity of data and calculation steps.In this study,we introduce a GPU-based parallel calculation method of a precise integration method(PIM) for seismic forward modeling.Compared with CPU single-core calculation,GPU parallel calculating perfectly keeps the features of PIM,which has small bandwidth,high accuracy and capability of modeling complex substructures,and GPU calculation brings high computational efficiency,which means that high-performing GPU parallel calculation can make seismic forward modeling closer to real seismic records. | Jingyu Li Genyang Tang Tianyue Hu | 2010 | Earthquake Science2010，23，4： | 2 |
| 12 | Metastable liquid properties and rapid crystal growth of Ti-Ni-Al alloy investigated by electrostatic levitation and molecular dynamics simulation显示文摘Electrostatic levitation technique and molecular dynamics simulation were performed to investigate the thermophysical properties,liquid structure and crystal growth dependence on undercooling of Ti_(85)Ni_(10)Al_(5) alloy.The liquid Ti_(85)Ni_(10)Al_(5) alloy was substantially undercooled up to 335 K(0.18T_(L)).As undercooling increased,the potential energy of the liquid alloy decreased and the alloy entered into a high metastable state.At this state,the atoms tended to bond with each other and the clusters were inclined to convert into high-coordinated clusters,as confirmed by the fraction of the high-coordinated clusters variation.The enlarged clusters and enhanced local structure stability contributed to the increase of the thermophysical parameters and crystal growth velocity,and eventually dendrite refinement.The density,the specific heat and the surface tension of liquid alloy exhibited a linear relation with temperature and the shear viscosity of liquid alloy showed exponential variation which showed good agreement with the calculation results by molecular dynamics simulation.The growth velocity first increased slowly and then dramatically once the undercooling exceeded the threshold.The achieved maximum crystal growth velocity was 12.4 m s^(−1) and it was up to 326 times of the value at 94 K undercooling. | XIAO RuiLin RUAN Ying LIN MaoJie QIN JingYu LI Hui HU Liang WEI BingBo | 2021 | Science China(Technological Sciences)2021，64，10： | 2 |
| 13 | RuO_(2) clusters derived from bulk SrRuO_(3):Robust catalyst for oxygen evolution reaction in acid显示文摘Developing highly efficient oxygen evolution reaction(OER)catalyst for the acidic corrosive operating conditions is a challenging task.Herein,we report the synthesis of uniform RuO_(2)clusters with~2 nm in size via electrochemical leaching of Sr from SrRuO_(3) ceramic in acid.The RuO_(2)clusters exhibit ultrahigh OER activity with overpotential of~160 mV at 10 mA·cm_(geo)^(−2) in 1.0 M HClO4 solution for 30-h testing.The extended X-ray absorption fine structure measurement reveals enlarged Jahn-Teller distortion of Ru-O octahedra in the RuO_(2)clusters compared to its bulk counterpart.Density function theory calculations show that the enhanced Jahn-Teller distortion can improve the intrinsic OER activity of RuO_(2). | Muwei Ji Xin Yang Shengding Chang Wenxing Chen Jin Wang Dongsheng He Yao Hu Qian Deng You Sun Bo Li Jingyu Xi Tomoaki Yamada Jiatao Zhang Hai Xiao Caizhen Zhu Jia Li Yadong Li | 2022 | Nano Research2022，15，3： | 2 |
| 14 | Face recognition based on uncorrelated discriminant transformation 显示文摘 | JIN Zhong YANG Jingyu HU Zhongshan LOU Zhen | 2001 | Pattern Recognition2001，34，7： | 1 |
| 15 | A Preliminary Investigation of Pathogenic Fungi from Lotus(Nelumbo nucifera Gaertn)in Nanchang City显示文摘[Objectives]Lotus(Nelumbo nucifera Gaertn)is an economically important aquatic plant in China.Fungal disease is a serious problem in lotus cultivation.In this study,the pathogenic fungi on lotus in Nanchang City were investigated to lay the foundation for the disease control.[Methods]Lotus leaves and stems in ponds of Nanchang City were collected,the fungi on leave/stem spots were isolated and purified.Colonies morphological characters and ITS sequences were used to identify the strains.[Results]49 strains were isolated and identified to 20 species,belonging to 12 genera.[Discussion]15 species may firstly be reported on lotus in this study,i.e.,Alternaria angustiovoidea,Alternaria compacta,Alternaria ricini,Alternaria tenuissima,Arthrinium arundinis,Botryosphaeria dothidea,Curvularia spicifera,Diaporthe australiana,Diaporthe eres,Diaporthe tectonae,Epicoccum nigrum,Fusarium fujikuroi,Neofusicoccum parvum,Nigrospora sphaerica,and Phomopsis eucommii. | HUANG Jingyue TANG Hong GU Bo TU Juanjuan HUANG Jiawen ZHONG Cheng HU Dianming | 2021 | 生物灾害科学2021，44，2： | 1 |
| 16 | Evaluation of the effect of vacuum on mold filling in the magnesium EPC process显示文摘 | Zili Liu Jingyu Hu Wenjiang Ding | 2002 | Journal of Materials Processing Technology2002，120，： | 1 |
| 17 | Metabolic reprogramming and redox adaptation in sorafenib-resistant leukemia cells:detected by untargeted metabolomics and stable isotope tracing analysis显示文摘Background:Internal tandem duplications(ITD)within the juxtamembrane domain of FMS-like tyrosine kinase 3(FLT3)represent a poor prognostic indicator in acute myeloid leukemia(AML).Therapeutic benefits of tyrosine kinase inhibitors,such as sorafenib,are limited due to the emergence of drug resistance.While investigations have been con-ducted to improve the understanding of the molecular mechanisms underlying the resistance to this FLT3 inhibitor,a profile of cell functioning at the metabolite level and crosstalk between metabolic pathways has yet to be created.This study aimed to elucidate the alteration of metabolomic profile of leukemia cells resistant to the FLT3 inhibitor.Methods:We established two sorafenib-resistant cell lines carrying FLT3/ITD mutations,namely the murine BaF3/ITD-R and the human MV4-11-R cell lines.We performed a global untargeted metabolomics and stable isotope-labe-ling mass spectrometry analysis to identify the metabolic alterations relevant to the therapeutic resistance.Results:The resistant cells displayed fundamentally rewired metabolic profiles,characterized by a higher demand for glucose,accompanied by a reduction in glucose flux into the pentose phosphate pathway(PPP);and by an increase in oxidative stress,accompanied by an enhanced glutathione synthesis.We demonstrated that the highest scoring network of altered metabolites in resistant cells was related to nucleotide degradation.A stable isotope tracing experiment was performed and the results indicated a decrease in the quantity of glucose entering the PPP in resistant cells.Further experiment suggested that the inhibition of major enzymes in the PPP consist of glucose-6-phosphate dehydrogenase deficiency(G6PD)in the oxidative arm and transketolase(TKT)in the non-oxidative arm.In addition,we observed that chronic treatment with sorafenib resulted in an increased oxidative stress in FLT3/ITD-positive leu-kemia cells,which was accompanied by decreased cell proliferation and an enhanced antioxidant response.Conclusions:Our data regarding comparative metabolomics characterized a distinct metabolic and redox adaptation that may contribute to sorafenib resistance in FLT3/ITD-mutated leukemia cells. | Xin You Weiye Jiang Wenhua Lu Hui Zhang Tiantian Yu Jingyu Tian Shijun Wen Guillermo Garcia-Manero Peng Huang Yumin Hu | 2019 | Cancer Communications2019，39，1： | 1 |
| 18 | Determination of trace elements in superalloy by ICP-MS显示文摘 | Hu Jingyu Wang Haizhou | 2001 | Microchimica Acta2001，137，34： | 1 |
| 19 | Determination of trace silverin supralloys and steel by ICP-MS 显示文摘 | Hu Jingyu Liu Zheng Wang Haizhou | 2002 | Anal Chim Acta2002，451，2： | 1 |
| 20 | Determination of Trace Elements in super alloy by ICP-MS显示文摘 | Hu Jingyu Wang Haizhou | 2001 | Mikrochim Acta2001，137，： | 1 |
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