|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Characterization of Basic Nitrogen Aromatic Species Obtained during Fluid Catalytic Cracking by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry显示文摘The basic-nitrogen aromatic compounds in feedstocks and liquid products from the micro-reactor and soluble components of coke obtained during fluid catalytic cracking (FCC) process were analyzed by the micro-electrospray ionization (ESI) 9.4T Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with an average mass resolving power of 300 000 at a mass range of 100-1 200. The analytical results revealed that the coker gas oil (CGO) contained a higher abundance of basic-nitrogen aromatic compounds with the type of -5N to -9N compared with those in deasphalted oil (DAO) and mixed FCC feedstock. After catalytic cracking, the abundance of lowly condensed basic-nitrogen aromatic compounds was much less than those of highly condensed aromatics in the liquid products, with the carbon number mainly ranging from 6 to 25 and the average carbon number of the side-chains equating to 1-5. On the contrary, with respect to the soluble components of coke, the abundance of lowly condensed basic-nitrogen aromatic compounds was more than those of highly condensed aromatics, and the carbon number ranged from 12 to 30, which was much smaller than that of the mixed FCC feedstock but slightly larger than that of the cracked liquid products. These results have provided some fundamental information on FCC process. | Liu Yingrong Wang Wei Hu Qiuling Zhu Yuxia Deng Jinghui Tian Songbai | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，2： | 8 |
| 2 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 3 | A Multicenter Application and Evaluation of the Oxford Classification of IgA Nephropathy in Adult Chinese Patients显示文摘 | Cai-Hong Zeng Weibo Le Zhaohui Ni Minfang Zhang Lining Miao Ping Luo Rong Wang Zhimei Lv Jianghua Chen Jiong Tian Nan Chen Xiaoxia Pan Ping Fu Zhangxue Hu Lining Wang Qiuling Fan Hongguang Zheng Dewei Zhang Yaping Wang Yanhong Huo Hongli Lin Shuni Chen Sh | 2012 | American Journal of Kidney Diseases2012，，5： | 2 |
| 4 | Regulation of mTORC1 by amino acids in mammalian cells: A general picture of recent advances显示文摘The mechanistic target of rapamycin complex 1(mTORC1)integrates various types of signal inputs,such as energy,growth factors,and amino acids to regulate cell growth and proliferation mainly through the 2 direct downstream targets,eukaryotic translation initiation factor 4 E-binding protein 1(4 EBP1)and ribosomal protein S6 kinase 1(S6 K1).Most of the signal arms upstream of mTORCl including energy status,stress signals,and growth factors converge on the tuberous sclerosis complex(TSC)-Ras homologue enriched in brain(Rheb)axis.Amino acids,however,are distinct from other signals and modulate mTORCl using a unique pathway.In recent years,the transmission mechanism of amino acid signals upstream of mTORCl has been gradually elucidated,and some sensors or signal transmission pathways for individual amino acids have also been discovered.With the help of these findings,we propose a general picture of recent advances,which demonstrates that various amino acids from lysosomes,cytoplasm,and Golgi are sensed by their respective sensors.These signals converge on mTORCl and form a huge and complicated signal network with multiple synergies,antagonisms,and feedback mechanisms. | Shizhe Zhang Xueyan Lin Qiuling Hou Zhiyong Hu Yun Wang Zhonghua Wang | 2021 | Animal Nutrition2021，，4： | 2 |
| 5 | Responses in splanchnic and mammary amino acid metabolism to short-term graded removal of methionine in lactating goats显示文摘Four multi-catheterized lactating goats were used in a 4×4 Latin square experiment to investigate the responses of amino acid metabolism in portal-drained viscera(PDV),liver,and mammary glands to short-term varying supplies of methionine(Met).During the last 45 h in each experimental period,goats were fasted for 12 h and then abomasally infused with an amino acid(AA)mixture plus glucose for 33 h.Treatments consisted of graded removal of Met from an infused AA mixture to achieve Met content in the infusate of 100%(complete),60%,30%,or 0%that in casein.Graded Met removal decreased the pro-duction of milk,milk protein,lactose,and fat linearly whilst also decreasing arterial Met concentration linearly(P<0.05).Meanwhile,net PDV uptake and liver removal of Met decreased linearly(P<0.05)due to decreased Met affinity of PDV and liver(P<0.05).Net mammary uptake of Met(P>0.1)was maintained as Met supply declined.This was achieved through increased mammary affinity(P<0.05)and increased mammary blood flow(P<0.05)totally offsetting the negative effect of decreased circu-lating Met concentration.Graded removal of Met from the infusate linearly decreased mammary uptake-to-milk output ratios of Met(P<0.05)and tended to decrease essential amino acid(EAA)linearly(0.05 | Yantao Li Mark D.Hanigan Xueyan Lin Zhiyong Hu Zhengui Yan Qiuling Hou Yun Wang Zhonghua Wang | 2023 | Animal Nutrition2023，，2： | 0 |
| 6 | Prediction of fibril formation by early-stage amyloid peptide aggregation显示文摘Amyloid fibrils are found in systemic amyloidosis diseases such as Alzheimer's disease,Parkinson's disease,and type II diabetes.Currently,these diseases are diagnosed by observation of fibrils or plaques,which is an ineffective method for early diagnosis and treatment of disease.The goal of this study was to develop a simple and quick method to predict the possibility and speed of fibril formation before its occurrence.Oligomers generated from seven representative peptide segments were first isolated and detected by ion-mobility mass spectrometry(IM-MS).Then,their assemblies were disrupted using formic acid(FA).Interestingly,oligomers that showed small ion intensity changes upon FA addition had rapid fibril formation.By contrast,oligomers that had large ion intensity changes generated fibrils slowly.Two control peptides(aggregation/no fibrils and no aggregation/no fibrils)did not show changes in their ion intensities,which confirmed the ability of this method to predict amyloid formation.In summary,the developed method correlated MS intensity ratio changes of peptide oligomers on FA addition with their amyloid propensities.This method will be useful for monitoring peptide/protein aggregation behavior and essential for their mechanism studies. | Jiaojiao Hu Huiyong Sun Haiping Hao Qiuling Zheng | 2020 | Journal of Pharmaceutical Analysis2020，10，2： | 0 |
| 7 | IGF2-NR4A2 Signaling Regulates Macrophage Subtypes to Attenuate Liver Cirrhosis显示文摘Background and Aims:Liver cirrhosis can lead to liver failure and eventually death.Macrophages are the main contributors to cirrhosis and have a bidirectional role in regulating matrix deposition and degradation.Macrophage-based cell therapy has been developed as an alternative to liver transplantation.However,there is insufficient evidence regarding its safety and efficacy.In this study,we aimed to explore the effect of combining insulin-like growth factor 2(IGF2)with bone marrow-derived macrophages(BMDMs)to treat mice with liver cirrhosis.Methods:We assessed liver inflammation,fibrosis regression,liver function,and liver regeneration in mice with CCl4-induced cirrhosis and treated with BMDM only or IGF2+BMDM.We performed in vitro experiments in which activated hepatic stellate cells(HSCs)were co-cultured with macrophages in the presence or absence of IGF2.The polarity of macrophages and the degree of inhibition of HSCs were examined.The effect of IGF2 on macrophages was also verified by the overexpression of IGF2.Results:Combining IGF2 with BMDM reduced liver inflammation and fibrosis and increased hepatocyte proliferation.Combining IGF2 with BMDM was more effective than using BMDM alone.In vitro experiments demonstrated that IGF2 inhibited HSCs activation by upregulating NR4A2 to promote the anti-inflammatory macrophages phenotype.IGF2 also increased the synthesis of matrix metalloproteinases(MMPs)by macrophages,which may explain why administering IGF2 combined with BMDM was more effective than administering BMDM only.Conclusions:Our study provides a theoretical basis for the future use of BMDM-based cell therapy to treat liver cirrhosis. | Lichao Yao Xue Hu Mengqin Yuan Qiuling Zhang Pingji Liu Lian Yang Kai Dai Yingan Jiang | 2023 | Journal of Clinical and Translational Hepatology2023，11，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员