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| 1 | Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice显示文摘Dear Editor,Hearing loss is the most common sensory disorder in the world.Among cases of non-syndromic hearing loss,which account for 70%of all cases of genetic hearing loss,around 80%of cases arise from autosomal recessive loss-of-function mutations that require repair,rather than disruption,of the mutant allele.1 CRISPR/Cas9-mediated homology-directed repair(HDR)-based therapies have the potential to cure many genetic diseases because this class of therapeutics can achieve arbitrary base changes as well as the insertion or deletion of DNA fragments/However. | Xi Gu Xinde Hu Daqi Wang Zhijiao Xu Fang Wang Di Li Geng-lin Li Hui Yang Huawei Li Erwei Zuo Yilai Shu | 2022 | Cell Research2022，32，7： | 3 |
| 2 | Retro-enantio isomer of angiopep-2 assists nanoprobes across the blood-brain barrier for targeted magnetic resonance/fluorescence imaging of glioblastoma显示文摘Glioblastoma(GBM),one of the most common primary intracranial malignant tumours,is very difficult to be completely excised by surgery due to its irregular shape.Here,we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles(SPIONs)modified with indocyanine(Cy7)molecules and peptides(ANG orDANG)to locate malignant gliomas and guide accurate excision.Both peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro. | Ruoxi Xie Zijun Wu Fanxin Zeng Huawei Cai Dan Wang Lei Gu Hongyan Zhu Su Lui Gang Guo Bin Song Jinxing Li Min Wu Qiyong Gong | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 3 |
| 3 | Astaxanthine attenuates cisplatin ototoxicity in vitro and protects against cisplatin-induced hearing loss in vivo显示文摘Astaxanthine(AST) has important biological activities including antioxidant and antiinflammatory effects that could alleviate neurological and heart diseases, but its role in the prevention of cisplatin-induced hearing loss(CIHL) is not yet well understood. In our study, a steady interaction between AST and the E3 ligase adapter Kelch-like ECH-associated protein 1, a predominant repressor of nuclear factor erythroid 2-related factor 2(NRF2), was performed and tested via computer molecular docking and dynamics. AST protected against cisplatin-induced ototoxicity via NRF2 mediated pathwayusing quantitative PCR and Western blotting. The levels of reactive oxygen species(ROS) and mitochondrial membrane potential revealed that AST reduced ROS overexpression and mitochondrial dysfunction.Moreover, AST exerted anti-apoptosis effects in mouse cochlear explants using immunofluorescence staining and HEI-OC1 cell lines using quantitative PCR and Western blotting. Finally, AST combined with poloxamer was injected into the middle ear through the tympanum, and the protection against CIHL was evaluated using the acoustic brain stem test and immunofluorescent staining in adult mice. Our results suggest that AST reduced ROS overexpression, mitochondrial dysfunction, and apoptosis via NRF2-mediated pathway in cisplatin-exposed HEI-OC1 cell lines and mouse cochlear explants, finally promoting cell survival. Our study demonstrates that AST is a candidate therapeutic agent for CIHL. | Benyu Nan Zirui Zhao Kanglun Jiang Xi Gu Huawei Li Xinsheng Huang | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 2 |
| 4 | Salubrinal protects against Clostridium difficile toxin B-induced CT26 cell death显示文摘Clostridium 顽固(C。顽固) 被认为在动物和人是联系抗菌素的腹泻和 pseudomembranous 大肠炎的主要原因。C 的流行。自从 2000,顽固感染(CDI ) 一直在增加。C 的二外毒素。顽固,毒素 A (TcdA ) 和毒素 B (TcdB ) , CDI 的主要毒力因素,能在宿主 cytosol 导致 Rho GTPases 的 glucosylation,正在导致细胞词法变化,细胞 apoptosis,和细胞死亡。导致 TcdB 的房间死亡的机制被调查十年了,但是它仍然不完全被理解。TcdB 经由在 CT26 房间线(BALB/C 老鼠冒号肿瘤房间) 表明小径的 PERK-eIF2 导致 endoplasmic 蜂窝胃应力,这被报导了。在这研究,我们发现了那 salubrinal, eIF2 dephosphorylation 的一个选择禁止者,高效地保护 CT26 房间线免于导致 TcdB 的房间死亡并且试着探索在这保护的效果位于下面的机制。我们的结果证明那 salubrinal 保护 CT26 房间免受的伤害调停 TcdB 细胞毒素并且 cytopathic 效果,经由 caspase-9-dependent 小径, eIF2 发信号小径,和 autophagy 禁止暴露毒素的房间的 apoptosis 和死亡。这些调查结果将对 CDI 治疗的发展有用。 | Shuyi Chen Chunli Sun Huawei Gu Haiying Wang Shan Li Yi Ma Jufang Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 2 |
| 5 | Experimental study of stator clocking effects in an axial compressor显示文摘 | CHEN Fu GU Zhonghua LU Huawei | | 0，，04： | 1 |
| 6 | High-mobility group box 1 protein contributes to the immunogenicity of rTcdB-treated CT26 cells显示文摘 | Huawei Gu Ji Liu Shuyi Chen Haonan Qi Kan Shi Shan Li Yi Ma Jufang Wang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 1 |
| 7 | First Principles Study-D8_8 Lattice Stability ofLow-Rate Metalloid Ti_5Si_3显示文摘First principles study is reported for the band structures, site and angular momentum decomposed density of states. and the electronic charge density distributions in high-temperature structural materials D88-Ti5Si3 with the addition of low-rate metalloid: carbon, boron, nitrogen and oxygen. It shows that how the addition of metalloid atoms can stabilize Nowotny phase of Ti,Si3 from the viewpoint of the electron structure. | Hong Lihua Ye Yiying(Department of Aanalysis and Measurement Science, Wuhan University, Wuhan 430072, China)Gu Huawei(Henan Basic and Applied Science Research Institute, Zhengzhou University, Zhengzhou 450052, China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，4： | 0 |
| 8 | pH-Responsive and Buffering Macromolecule Aqueous Absorbent and Mathematic Model-Based Feasibility Evaluation for SO_2 Capture显示文摘An organic macromolecule, poly(1-vinylimidazole), with an appropriate polymerization degree was proposed and mixed with water to form a novel aqueous absorbent for SO_2 capture. This aqueous solution absorbent has the advantages of simple preparation, good physicochemical properties, environment-friendliness, high ability in deep removal of SO_2, and excellent reusability. Moreover, pH-responsive behavior, pH buffering absorption mechanism, and their synergistic effect on absorption performance were revealed. The solubilities of SO_2 in the absorbent were measured in detail, and the results demonstrated excellent absorption capacity and recyclability. Then, mathematic models that describe SO_2 absorption equilibrium were established, and the corresponding parameters were estimated. More importantly, on the basis of model and experimental data, the absorption and desorption could maintain high efficiency within a wide operating region. In summary, this work provided a low-cost, efficient, and unique absorbent for SO_2 capture and verified its technical feasibility in industrial application. | Wei Feng Wenhao Gu Lühong Zhang Xiaowei Tantai Bin Jiang Huawei Yang Hongjie Zhang | 2019 | Transactions of Tianjin University2019，25，3： | 0 |
| 9 | Small protein Cgl2215 enhances phenolic tolerance by promoting MytA activity in Corynebacterium glutamicum显示文摘Corynebacterium glutamicum is a promising chassis microorganism for the bioconversion of lignocellulosic biomass owing to its good tolerance and degradation of the inhibitors generated in lignocellulosic pretreatments.Among the identified proteins encoded by genes within the C.glutamicum genome,nearly 400 are still functionally unknown.Based on previous transcriptome analysis,we found that the hypothetical protein gene cgl2215 was highly upregu-lated in response to phenol,ferulic acid,and vanillin stress.The cgl2215 deletion mutant was shown to be more sensitive than the parental strain to phenolic compounds as well as other environmental factors such as heat,ethanol,and oxidative stresses.Cgl2215 interacts with C.glutamicum mycoloyltransferase A(MytA)and enhances its in vitro esterase activity.Sensitivity assays of theΔmytA andΔcgl2215ΔmytA mutants in response to phenolic stress estab-lished that the role of Cgl2215 in phenolic tolerance was mediated by MytA.Furthermore,transmission electron microscopy(TEM)results showed that cgl2215 and mytA deletion both led to defects in the cell envelope structure of C.glutamicum,especially in the outer layer(OL)and electron-transparent layer(ETL).Collectively,these results indicate that Cgl2215 can enhance MytA activity and affect the cell envelope structure by directly interacting with MytA,thus playing an important role in resisting phenolic and other environmental stresses. | Huawei Gu Xinwei Hao Ruirui Liu Zhenkun Shi Zehua Zhao Fu Chen Wenqiang Wang Yao Wang Xihui Shen | 2022 | Stress Biology2022，2，1： | 0 |
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