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| 1 | PDCD5 regulates iNKT cell terminal maturation and iNKT1 fate decision显示文摘Invariant natural killer T1(iNKT1)cells are characterized by the preferential expression of T-box transcription factor T-bet(encoded by Tbx21)and the production of cytokine IFN-γ,but the relationship between the developmental process and iNKT1 lineage diversification in the thymus remains elusive.We report in the present study a crucial role of programmed cell death 5(PDCD5)in iNKT cell terminal maturation and iNKT1 fate determination.Mice with T cell-specific deletion of PDCD5 had decreased numbers of thymic and peripheral iNKT cells with a predominantly immature phenotype and defects in response toα-galactosylceramide.Loss of PDCD5 also selectively abolished the iNKT1 lineage by reducing T-bet expression in iNKT cells at an early thymic developmental stage(before CD44 upregulation).We further demonstrated that TOX2,one of the high mobility group proteins that was highly expressed in iNKT cells at stage 1 and could be stabilized by PDCD5,promoted the permissive histone H3K4me3 modification in the promoter region of Tbx21.These data indicate a pivotal and unique role of PDCD5/TOX2 in iNKT1 lineage determination.They also suggest that the fate of iNKT1 may be programmed at the developmental stage of iNKT cells in the thymus. | Ke Wang Xinwei Zhang Yifan Wang Gaowen Jin Mingyang Li Shusong Zhang Jie Hao Rong Jin Xiaojun Huang Hounan Wu Jun Zhang Yingyu Chen Qing Ge | 2019 | Cellular & Molecular Immunology2019，16，9： | 4 |
| 2 | A Novel Artificial Neuron-Like Gas Sensor Constructed from CuS Quantum Dots/Bi2S3 Nanosheets显示文摘Real-time rapid detection of toxic gases at room temperature is particularly important for public health and environmental monitoring.Gas sensors based on conventional bulk materials often suffer from their poor surface-sensitive sites,leading to a very low gas adsorption ability.Moreover,the charge transportation efficiency is usually inhibited by the low defect density of surface-sensitive area than that in the interior.In this work,a gas sensing structure model based on CuS quantum dots/Bi_(2)S_(3) nanosheets(CuS QDs/Bi_(2)S_(3) NSs)inspired by artificial neuron network is constructed.Simulation analysis by density functional calculation revealed that CuS QDs and Bi_(2)S_(3) NSs can be used as the main adsorption sites and charge transport pathways,respectively.Thus,the high-sensitivity sensing of NO_(2) can be realized by designing the artificial neuron-like sensor.The experimental results showed that the CuS QDs with a size of about 8 nm are highly adsorbable,which can enhance the NO_(2) sensitivity due to the rich sensitive sites and quantum size effect.The Bi_(2)S_(3) NSs can be used as a charge transfer network channel to achieve efficient charge collection and transmission.The neuron-like sensor that simulates biological smell shows a significantly enhanced response value(3.4),excellent responsiveness(18 s)and recovery rate(338 s),low theoretical detection limit of 78 ppb,and excellent selectivity for NO_(2).Furthermore,the developed wearable device can also realize the visual detection of NO2 through real-time signal changes. | Xinwei Chen Tao Wang Jia Shi Wen Lv Yutong Han Min Zeng Jianhua Yang Nantao Hu Yanjie Su Hao Wei Zhihua Zhou Zhi Yang Yafei Zhang | 2022 | Nano-Micro Letters2022，14，1： | 3 |
| 3 | Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice显示文摘Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(Pi)starvation.However,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of Pi and nitrogen remain largely unclear.Here,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental responses.We found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-Pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in rice.Interestingly,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under Pi deficiency.We also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote Pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in rice.Importantly,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus conditions.Taken together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to Pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-Pi environments. | Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang Liquan Kou Xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang | 2023 | Molecular Plant2023，16，11： | 2 |
| 4 | Recognition of high-specificity hERG K+ channel inhibitor-induced arrhythmia in cardiomyocytes by automated template matching显示文摘Cardiovascular disease(CVD)is the number one cause of death in humans.Arrhythmia induced by gene mutations,heart disease,or hERG K+channel inhibitors is a serious CVD that can lead to sudden death or heart failure.Conventional cardiomyocyte-based biosensors can record extracellular potentials and mechanical beating signals.However,parameter extraction and examination by the naked eye are the traditional methods for analyzing arrhythmic beats,and it is difficult to achieve automated and efficient arrhythmic recognition with these methods.In this work,we developed a unique automated template matching(ATM)cardiomyocyte beating model to achieve arrhythmic recognition at the single beat level with an interdigitated electrode impedance detection system.The ATM model was established based on a rhythmic template with a data length that was dynamically adjusted to match the data length of the target beat by spline interpolation.The performance of the ATM model under long-term astemizole,droperidol,and sertindole treatment at different doses was determined.The results indicated that the ATM model based on a random rhythmic template of a signal segment obtained after astemizole treatment presented a higher recognition accuracy(100%for astemizole treatment and 99.14%for droperidol and sertindole treatment)than the ATM model based on arrhythmic multitemplates.We believe this highly specific ATM method based on a cardiomyocyte beating model has the potential to be used for arrhythmia screening in the fields of cardiology and pharmacology. | Hao Wang Hongbo Li Xinwei Wei Tao Zhang Yuting Xiang Jiaru Fang Peiran Wu Xi Xie Ping Wang Ning Hu | 2021 | Microsystems & Nanoengineering2021，7，2： | 2 |
| 5 | TRAF3IP3 at the trans-Golgi network regulates NKT2 maturation via the MEK/ERK signaling pathway显示文摘Thymic natural killer T(NKT)2 cells are a subset of invariant NKT cells with PLZF^(hi)GATA3^(hi)IL-4^(+).The differentiation of NKT2 cells is not fully understood.In the present study,we report an important role of TRAF3-interacting protein 3(TRAF3IP3)in the functional maturation and expansion of committed NKT2s in thymic medulla.Mice with T-cell-specific deletion of TRAF3IP3 had decreased thymic NKT2 cells,decreased IL-4-producing peripheral iNKTs,and defects in response toα-galactosylceramide.Positive selection and high PLZF expression in CD24^(+)CD44^(−) and CCR7^(+)CD44^(−) immature iNKTs were not affected.Only CD44^(hi)NK1.1^(−) iNKTs in Traf3ip3^(−/−) mice showed reduced expression of Egr2,PLZF,and IL-17RB,decreased proliferation,and reduced IL-4 production upon stimulation.This Egr2 and IL-4 expression was augmented by MEK1/ERK activation in iNKTs,and TRAF3IP3 at the trans-Golgi network recruited MEK1 and facilitated ERK phosphorylation and nuclear translocation.LT βR-regulated bone marrow-derived nonlymphoid cells in the medullary thymic microenvironment were required for MEK/ERK activation and NKT2 maturation.These data demonstrate an important functional maturation process in NKT2 differentiation that is regulated by MEK/ERK signaling at the trans-Golgi network. | Xinwei Zhang Ke Wang Weijia Zhao Li Cao Shusong Zhang Rong Jin Xiuyuan Sun Jie Hao Xiaojun Huang Mingzhao Zhu Hounan Wu Hongshan Zhao Qing Ge | 2020 | Cellular & Molecular Immunology2020，17，4： | 2 |
| 6 | Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy——lysosomal pathway显示文摘Nonalcoholic fatty liver disease(NAFLD) is characterized by hepatic steatosis and insulin resistance and there are currently no approved drugs for its treatment.Hyperactivation of mTOR complex1(mTORCl) and subsequent impairment of the transcription factor EB(TFEB)-mediated autophagy-lysosomal pathway(ALP) are implicated in the development of NAFLD.Accordingly,agents that augment hepatic TFEB transcriptional activity may have therapeutic potential against NAFLD.The objective of this study was to investigate the effects of nuciferine,a major active component from lotus leaf,on NAFLD and its underlying mechanism of action.Here we show that nuciferine activated ALP and alleviated steatosis,insulin resistance in the livers of NAFLD mice and palmitic acid-challenged hepatocytes in a TFEB-dependent manner.Mechanistic investigation revealed that nuciferine interacts with the Ragulator subunit hepatitis B X-interacting protein and impairs the interaction of the Ragulator complex with Rag GTPases,thereby suppressing lysosomal localization and activity of mTORC1,which activates TFEB-mediated ALP and further ameliorates hepatic steatosis and insulin resistance.Our present results indicate that nuciferine may be a potential agent for treating NAFLD and that regulation of the mTORCl-TFEB-ALP axis could represent a novel pharmacological strategy to combat NAFLD. | Xiliang Du Chiara Di Malta Zhiyuan Fang Taiyu Shen Xiaodi Niu Meng Chen Bo Jin Hao Yu Lin Lei Wenwen Gao Yuxiang Song Zhe Wang Chuang Xu Zhijun Cao Guowen Liu Xinwei Li | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 2 |
| 7 | Metal contamination incampus dust of Xi) an, China: a study based on multivariatestatistics and spatial distribution显示文摘 | CHEN Hao’LU Xinwei LI L Y | 2014 | Science of the Total En-vironment2014，4841，： | 1 |
| 8 | Effect of CeO2 on High Temperature Carburization Behavior of Mn-Cr-O Spinel and Chromium Oxide 显示文摘 | Li Hao Cui Xinwei Chen Weixing | 2010 | J Electrochem Soc2010，157，10： | 1 |
| 9 | Bubble Formation on Superhydrophobic-IVlicropatterned Copper Surfaces 显示文摘 | WANG Xinwei ZHAO Siwei WANG Hao | 2012 | Applied Thermal Engineering2012，35，11： | 1 |
| 10 | Observa- tion of Nucleate Boiling on a Fine Copper Wire V~ith Su- perhydrophobic Mieropatterns 显示文摘 | WANG Xinwei SONG Yongxin 'WANG Hao | 2012 | China Physics Letters2012，29，11： | 1 |
| 11 | Assessment of metals pollution and health risk in dust from nursery schools in Xi’an, China显示文摘 | Xinwei Lu Xiaolan Zhang Loretta Y. Li Hao Chen | 2014 | Environmental Research2014，，： | 1 |
| 12 | Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis显示文摘 | Xiaohui Feng Hao Wen Zhaoxia Zhang Xinhua Chen Xudong Ma Jinping Zhang Xinwei Qi Helen Bradshaw Dominique Vuitton Philip S. Craig | 2009 | Acta Tropica2009，，2： | 1 |
| 13 | Cardiomyocyte electrical-mechanical synchronized model for high-content, dose-quantitative and time-dependent drug assessment显示文摘Cardiovascular diseases have emerged as a significant threat to human health.However,drug development is a time-consuming and costly process,and few drugs pass the preclinical assessment of safety and efficacy.The existing patch-clamp,Ca2+imaging,and microelectrode array technologies in cardiomyocyte models for drug preclinical screening have suffered from issues of low throughput,limited long-term assessment,or inability to synchronously and correlatively analyze electrical and mechanical signals.Here,we develop a high-content,dose-quantitative and time-dependent drug assessment platform based on an electrical-mechanical synchronized(EMS)biosensing system.This microfabricated EMS can record both firing potential(FP)and mechanical beating(MB)signals from cardiomyocytes and extract a variety of characteristic parameters from these two signals(FP–MB)for further analysis.This system was applied to test typical ion channel drugs(lidocaine and isradipine),and the dynamic responses of cardiomyocytes to the tested drugs were recorded and analyzed.The high-throughput characteristics of the system can facilitate simultaneous experiments on a large number of samples.Furthermore,a database of various cardiac drugs can be established by heat map analysis for rapid and effective screening of drugs.The EMS biosensing system is highly promising as a powerful tool for the preclinical development of new medicines. | Jiaru Fang Xinwei Wei Hongbo Li Ning Hu Xingxing Liu Dongxin Xu Tao Zhang Hao Wan Ping Wang Xi Xie | 2021 | Microsystems & Nanoengineering2021，7，2： | 1 |
| 14 | Cholesky-based model averaging for covariancematrix estimation显示文摘Estimation of large covariance matrices is of great importance in multivariate analysis.The modified Cholesky decomposition is a commonly used technique in covariance matrix estimation given a specific order of variables.However,information on the order of variables is often unknown,or cannot be reasonably assumed in practice.In this work,we propose a Choleskybased model averaging approach of covariance matrix estimation for high dimensional datawith proper regularisation imposed on the Cholesky factor matrix.The proposed method not only guarantees the positive definiteness of the covariance matrix estimate,but also is applicable in general situations without the order of variables being pre-specified.Numerical simulations are conducted to evaluate the performance of the proposed method in comparison with several other covariance matrix estimates.The advantage of our proposed method is further illustrated by a real case study of equity portfolio allocation. | Hao Zheng Kam-Wah Tsui Xiaoning Kang Xinwei Deng | 2017 | Statistical Theory and Related Fields2017，1，1： | 0 |
| 15 | Small protein Cgl2215 enhances phenolic tolerance by promoting MytA activity in Corynebacterium glutamicum显示文摘Corynebacterium glutamicum is a promising chassis microorganism for the bioconversion of lignocellulosic biomass owing to its good tolerance and degradation of the inhibitors generated in lignocellulosic pretreatments.Among the identified proteins encoded by genes within the C.glutamicum genome,nearly 400 are still functionally unknown.Based on previous transcriptome analysis,we found that the hypothetical protein gene cgl2215 was highly upregu-lated in response to phenol,ferulic acid,and vanillin stress.The cgl2215 deletion mutant was shown to be more sensitive than the parental strain to phenolic compounds as well as other environmental factors such as heat,ethanol,and oxidative stresses.Cgl2215 interacts with C.glutamicum mycoloyltransferase A(MytA)and enhances its in vitro esterase activity.Sensitivity assays of theΔmytA andΔcgl2215ΔmytA mutants in response to phenolic stress estab-lished that the role of Cgl2215 in phenolic tolerance was mediated by MytA.Furthermore,transmission electron microscopy(TEM)results showed that cgl2215 and mytA deletion both led to defects in the cell envelope structure of C.glutamicum,especially in the outer layer(OL)and electron-transparent layer(ETL).Collectively,these results indicate that Cgl2215 can enhance MytA activity and affect the cell envelope structure by directly interacting with MytA,thus playing an important role in resisting phenolic and other environmental stresses. | Huawei Gu Xinwei Hao Ruirui Liu Zhenkun Shi Zehua Zhao Fu Chen Wenqiang Wang Yao Wang Xihui Shen | 2022 | Stress Biology2022，2，1： | 0 |
| 16 | Transcriptional regulation of bark freezing tolerance in apple(Malus domestica Borkh.)显示文摘Freezing tolerance is a significant trait in plants that grow in cold environments and survive through the winter.Apple(Malus domestica Borkh.)is a cold-tolerant fruit tree,and the cold tolerance of its bark is important for its survival at low temperatures.However,little is known about the gene activity related to its freezing tolerance.To better understand the gene expression and regulation properties of freezing tolerance in dormant apple trees,we analyzed the transcriptomic divergences in the bark from 1-year-old branches of two apple cultivars,“Golden Delicious”(G)and“Jinhong”(H),which have different levels of cold resistance,under chilling and freezing treatments.“H”can safely overwinter below−30℃in extremely low-temperature regions,whereas“G”experiences severe freezing damage and death in similar environments.Based on 28 bark transcriptomes(from the epidermis,phloem,and cambium)from 1-year-old branches under seven temperature treatments(from 4 to−29°C),we identified 4173 and 7734 differentially expressed genes(DEGs)in“G”and“H”,respectively,between the chilling and freezing treatments.A gene coexpression network was constructed according to this expression information using weighted gene correlation network analysis(WGCNA),and seven biologically meaningful coexpression modules were identified from the network.The expression profiles of the genes from these modules suggested the gene regulatory pathways that are responsible for the chilling and freezing stress responses of“G”and/or“H.”Module 7 was probably related to freezing acclimation and freezing damage in“H”at the lower temperatures.This module contained more interconnected hub transcription factors(TFs)and cold-responsive genes(CORs).Modules 6 and 7 contained C-repeat binding factor(CBF)TFs,and many CBF-dependent homologs were identified as hub genes.We also found that some hub TFs had higher intramodular connectivity(KME)and gene significance(GS)than CBFs.Specifically,most hub TFs in modules 6 and 7 were activated at the beginning of the early freezing stress phase and maintained upregulated expression during the whole freezing stress period in“G”and“H”.The upregulation of DEGs related to methionine and carbohydrate biosynthetic processes in“H”under more severe freezing stress supported the maintenance of homeostasis in the cellular membrane.This study improves our understanding of the transcriptional regulation patterns underlying freezing tolerance in the bark of apple branches. | Yinghai Liang Shanshan Wang Chenhui Zhao Xinwei Ma Yiyong Zhao Jing Shao Yuebo Li Honglian Li Hongwei Song Hong Ma Hao Li Bingbing Zhang Liangsheng Zhang | 2020 | Horticulture Research2020，7，1： | 0 |
| 17 | Evaluating the efficacy and cardiotoxicity of EGFR-TKI AC0010 with a novel multifunctional biosensor显示文摘Non-small cell lung cancer(NSCLC)is a leading cause of cancer mortality worldwide.Although epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs)have dramatically improved the life expectancy of patients with NSCLC,concerns about TKI-induced cardiotoxicities have increased.AC0010,a novel third-generation TKI,was developed to overcome drug resistance induced by EGFR-T790M mutation.However,the cardiotoxicity of AC0010 remains unclear.To evaluate the efficacy and cardiotoxicity of AC0010,we designed a novel multifunctional biosensor by integrating microelectrodes(MEs)and interdigital electrodes(IDEs)to comprehensively evaluate cell viability,electrophysiological activity,and morphological changes(beating of cardiomyocytes).The multifunctional biosensor can monitor AC0010-induced NSCLC inhibition and cardiotoxicity in a quantitative,label-free,noninvasive,and real-time manner.AC0010 was found to significantly inhibit NCI-H1975(EGFR-L858R/T790M mutation),while weak inhibition was found for A549(wild-type EGFR).Negligible inhibition was found in the viabilities of HFF-1(normal fibroblasts)and cardiomyocytes.With the multifunctional biosensor,we found that 10μM AC0010 significantly affected the extracellular field potential(EFP)and mechanical beating of cardiomyocytes.The amplitude of EFP continuously decreased after AC0010 treatment,while the interval decreased first and then increased.We analyzed the change in the systole time(ST)and diastole time(DT)within a beating interval and found that the DT and DT/beating interval rate decreased within 1 h after AC0010 treatment.This result probably indicated that the relaxation of cardiomyocytes was insufficient,which may further aggravate the dysfunction.Here,we found that AC0010 significantly inhibited EGFR-mutant NSCLC cells and impaired cardiomyocyte function at low concentrations(10μM).This is the first study in which the risk of AC0010-induced cardiotoxicity was evaluated.In addition,novel multifunctional biosensors can comprehensively evaluate the antitumor efficacy and cardiotoxicity of drugs and candidate compounds. | Deming Jiang Xinwei Wei Yuxuan Zhu Yong Qiu Xin Liu Liubing Kong Fengheng Li Jingwen Liu Liujing Zhuang Hao Wan Kejing Ying Ping Wang | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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