|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Chinese deserts and sand fields in Last Glacial Maximum and Holocene Optimum显示文摘The Last Glacial Maximum (LGM, c. 26-16 ka) and the Holocene Optimum (HO, c. 9-5 ka) were characterized by cold-dry and warm-wet climates respectively in the recently geological Earth. How Chinese deserts and sand fields responded to these distinctive climatic changes is still not clear, however. To reconstruct environments of the deserts and sand fields during the LGM and HO is helpful to understand the forcing mechanisms of environment change in this arid region, and to test paleoclimatic modeling results. Through our long-term field and laboratory investigations, 400 optically stimulated luminescence (OSL) ages and more than 100 depositional records in the Chinese deserts and sand fields were obtained; on the basis of these data, we reconstruct spatial distributions of the deserts and sand fields during the LGM and HO. Our results show that the sand fields of Mu Us, Hunshandake, Horqin and Hulun Buir in northern and northeastern China had expanded 25%, 37%, 38% and 270%, respectively, during the LGM; the sand fields of Gonghe in the northeastern Qinghai-Tibetan Plateau had expanded 20%, and the deserts of Badain Jaran, Tengger in central northern China had expanded 39% and 29% separately during the LGM; the deserts of Taklimakan, Gurbantünggüt and Kumtag in northwestern China had expanded 10%-20% respectively, compared to their modern areas. On the other hand, all of the sand fields were nearly completely covered by vegetation during the HO; the deserts in northwestern and central northern China were reduced by around 5%-20% in area during this time. Lakes in this arid region were probably expanded during the HO but this conclusion needs more investigation. Compared with the geological distributions of deserts and sand fields, human activity has clearly changed (expanded) the area of active sand dunes at the present time. Our observations show that environmental conditions of Chinese deserts and sand fields are controlled by regional climate together with human activity. | LU HuaYu YI ShuangWen XU ZhiWei ZHOU YaLi ZENG Lin ZHU FangYing FENG Han DONG LiNa ZHUO HaiXin YU KaiFeng MASON Joseph WANG XiaoYong CHEN YingYong LU Qi WU Bo DONG ZhiBao QU JianJun WANG XunMing GUO ZhengTang | 2013 | Chinese Science Bulletin2013，58，23： | 40 |
| 2 | Carbon isotopic composition of modern soil and paleosol as a response to vegetation change on the Chinese Loess Plateau显示文摘The relationship between the carbon isotopic composition of paleosols and paleovegetation on the Loess Plateau is still unclear. One of the main reasons is that we are short of knowledge about the characteristics of the carbon isotopic composition of modern soil in this area. A preliminary investigation of the carbon isotopic compositions of the modern soil and the loess/paleosol sequence on the Loess Plateau shows that the carbon isotopic composition of modern soil is consistent with the distribution of modern plants on the Loess Plateau, where the ecosystem is dominated by a mixture of C4 and C3 plants. Comparing theδ13C values of modern soil and loess-paleosol sequences from the Xunyi profile, we conclude that C3 plants dominated the landscape during loess sediment stages, while C4 plants expanded during paleosol stages. | LIU Weiguo NING Youfeng AN Zhisheng WU Zhenghai LU Huayu CAO Yunning | 2005 | Science China Earth Sciences2005，48，1： | 24 |
| 3 | Crescentic dune migration and stabilization: Implications for interpreting paleo-dune deposits as paleoenvironmental records显示文摘Paleo-dune deposits have been widely used as a proxy indicator of past dune activity, which is further used to reconstruct paleoclimates. However, recent studies have critically examined the reproducibility of dune chronologies and the complexity of paleo-dune deposits as paleoenvironmental records. This paper addresses questions on the paleoenvironmental implications of dune chronostratigraphies that have been raised by those reviews, in the specific case of crescentic dunes, using a case study from the Mu Us dune field, north-central China. The processes of turn-over and stabilization of relatively small crescentic dunes are first investigated by observational evidence. In combination with the analysis of a simplified sand preservation model and stratigraphic records, the effect of dune morphodynamics on sand preservation is demonstrated. It is especially evident that thick, nearly instantaneously deposited sand units record dune stabilization near the very end of a dune activity episode, while thin sand units may signal the preservation of sand deposited earlier in episodes of activity. Interpreting the distribution of luminescence ages that indicate sand deposition over time is not as simple as assumed in some previous work. Low frequency of sand ages could indicate an interval of either dune field stabilization or extensive dune activity but poor sand preservation. A peak of sand age frequency likely represents a shift in dune field activity towards stabilization, not a peak of active dune extent, especially if it partially overlaps with an independently identified interval of stabilization(e.g. one recorded by paleosols). The nature and magnitude of these biases in the distribution of sand ages over time are strongly affected by the magnitude of net sand accumulation, which is in turn related to sand supply, transport capacity and sand availability, and ultimately climate change. Relatively short dune stabilization and turn-over time(10~1 to 10~2 yrs) indicate that dune geomorphic processes can quickly respond to short-term disturbance, but the chronology of that response must be interpreted in light of how those processes influence age distributions. | XU Zhiwei Joseph A. MASON LU Huayu YI Shuangwen ZHOU Yali WU Jiang HAN Zhiyong | 2017 | Journal of Geographical Sciences2017，27，11： | 7 |
| 4 | Spatial and temporal variations in prehistoric human settlement and their influencing factors on the south bank of the Xar Moron River, Northeastern China显示文摘 | Xin JIA Shuangwen YI Yonggang SUN Shuangye WU Harry F. LEE Lin WANG Huayu LU | 2017 | Frontiers of Earth Science2017，11，1： | 5 |
| 5 | Dual pH and glucose sensitive gel gated mesoporous silica nanoparticles for drug delivery显示文摘A low molecular weight gelator with dual pH and glucose sensitive moieties was synthesized. The gelator penetrated in the mesopores of silica nanoparticles(MSNs) and formed low molecular weight gel(LMWG) as gate to fabricate dual pH and glucose responsive nano drug delivery system. Antidiabetic drug was loaded in the gel caped MSNs, the drug release was responsive to the pH and glucose levels and the drug release could be controlled via the stimuli sensitivity of gel. | Wenxia Gao Yalong Hu Long Xu Miaochang Liu Huayue Wu Bin He | 2018 | Chinese Chemical Letters2018，29，12： | 4 |
| 6 | A photocleavable low molecular weight hydrogel for light-triggered drug delivery显示文摘A photocleavable low-molecular-weight hydrogelator (LMWG) was synthesized based on coumarin derivative.~1H NMR and UV spectroscopy study suggested that the gelator had good gelling ability, and the driving force for the gelation were hydrogen bonding and π-π stacking. This molecular hydrogel exhibited satisfied photocleavage at C-N bond in 7-amino coumarin with the light irradiation (365 nm,77.5 mW/cm^2). The promising photo-triggered drug release of antineoplastics cytarabine hydrochloride has been obtained, due to the photocleavage motived gel-sol transition. | Qihong Liu Hao Wang Guotao Li Miaochang Liu Jinchang Ding Xiaobo Huang Wenxia Gao Huayue Wu | 2019 | Chinese Chemical Letters2019，30，2： | 4 |
| 7 | Melon short internode(CmSi)encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling显示文摘Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic yield.At least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in melon.Here,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode elongation.Spatial-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode elongation.The expression level of CmSI was positively correlated with stem length in the different melon varieties examined.Ectopic expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both species.Phytohormone quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin signaling.Thus,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in melon.Our findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport. | Sen Yang Kaige Zhang Huayu Zhu Xiaojing Zhang Wenkai Yan Nana Xu Dongming Liu Jianbin Hu Yufeng Wu Yiqun Weng Luming Yang | 2020 | Horticulture Research2020，7，1： | 2 |
| 8 | Combining mannose receptor mediated nanovaccines and gene regulated PD-L1 blockade for boosting cancer immunotherapy显示文摘Tumor nanovaccines have potential applications in the prevention and treatment of malignant tumors.However,it remains a longstanding challenge in exploiting efficient nanocarriers for inducing potent specifically cellular immune responses.Toward this objective,we herein explore an intensive tumor immunotherapeutic strategy by combining mannosylated nanovaccines and gene regulated PD-L1 blockade for immune stimulation and killing activity.Here,we fabricate a mannose modified PLL-RT(Man-PLL-RT)mediated nanovaccines with dendritic cells(DCs)targeting capacity.Man-PLL-RT is capable of co-encapsulating with antigen(ovalbumin,OVA)and adjuvant(unmethylated cytosine-phosphate-guanine,CpG)by electrostatic interaction.This positively charged Man-PLL-RT/OVA/CpG nanovaccines can facilitate the endocytosis,maturation and cross presentation in DCs.However,the nanovaccines arouse limited inhibition of tumor growth,which is mainly due to the immunosuppressed microenvironment of tumors.Combining tumor nanovaccines with gene regulated PD-L1 blockade leads to an obvious tumor remission in B16F10 melanoma bearing mice.The collaborative strategy provides essential insights to boost the benefits of tumor vaccines by regulating the checkpoint blockade with gene therapy. | Jie Chen Huapan Fang Yingying Hu Jiayan Wu Sijia Zhang Yuanji Feng Lin Lin Huayu Tian Xuesi Chen | 2022 | Bioactive Materials2022，7，1： | 2 |
| 9 | Inversion of the asymmetry factor for desert areas of China显示文摘Deserts,which have high surface albedo and wide area,are important components of the earth system.It is very important for the research of surface radiation and energy balance to understand the anisotropic scattering of desert areas.The emergence and development of multi-angle remote sensing made possible the inversion of the anisotropic scattering of desert areas at the regional or global scale.Firstly,this paper explored the accuracy of the inversion of asymmetry factor using the Hapke model and the simulated single-and multi-phase MISR data.Based on the results,the asymmetry factor of representative surface of desert areas in northwestern China was retrieved.The values of the asymmetry factor retrieved from MISR data were compared with the values retrieved from the laboratory data.The results showed that the single-phase MISR data could be used for the inversion of asymmetry parameter of desert areas.The sign of the asymmetry parameter for the laboratory measurements was positive,which suggests that the surface of laboratory samples is forward scattering.The sign of the asymmetry parameter for MISR data was negative;that is,it is backscattering.The values of the asymmetry parameters retrieved from MISR data were related to the character of the land surface.At Loulan,where the surface was smoother than other sites,retrieved values exhibited the largest negative values of asymmetry factor,suggesting the strongest backscattering.The sand dune area of the Kumtag Desert,which has the greatest roughness,had only slightly negative asymmetry factor values.These findings indicated that at the sensor scale,a rough surface(e.g.,dunes) does not necessarily mean more backscattering than a smooth surface.This finding has significant implications for empirical methods(e.g.,using the normalized index of backward-scattered radiance minus forward-scattered radiance as an indicator of surface roughness),which should be used carefully for analyzing surface roughness from the remote sensing data. | WU YunZhao LU HuaYu LIU Qiang | 2010 | Science China Earth Sciences2010，53，4： | 2 |
| 10 | Labeling Dynamic XML Documents:An Order-Centric Approach显示文摘 | Liang Xu Tok Wang Ling Huayu Wu | 2012 | IEEE transactions on knowledge and data engineering2012，，24： | 1 |
| 11 | Mineralogy, major and trace element geochemistry of riverbed sedi- ments in the headwaters of the Yangtze, Tongtian River and Jinsha River显示文摘 | Wu Weihua Xu Shijun Lu Huayu Yang Jiedong Yin Hongwei Liu Wen | 2011 | Journal of Asian Earth Sciences2011，40，： | 1 |
| 12 | Exceptionally flame-retardant flexible polyurethane foam composites:synergistic effect of the silicone resin/graphene oxide coating显示文摘A facile strategy was developed to fabricate flexible polyurethane(PU)foam composites with exceptional flame retardancy.The approach involves the incorporation of graphene oxide(GO)into a silicone resin(SiR)solution,which is then deposited onto a PU foam surface via the dip-coating technique and cured.Fourier-transform infrared spectroscopy,scanning electron microscopy,and Raman spectroscopy measurements demonstrated that the SiR and GO were successfully coated onto the PU skeleton and the intrinsic porous structure of the PU foam remained intact.The effects of SiR and GO on the mechanical and thermal stability and flame retardancy of PU composites were evaluated through compression tests,thermogravimetric analysis,vertical combustion tests,and the limiting oxygen index.The measurement results revealed that the composites(PU@SiR-GO)showed superior flame retardancy and thermal and mechanical stability compared to pristine PU or PU coated with SiR alone.The mechanical and thermal stability and the flame-retardant properties of the PU composites were enhanced significantly with increasing GO content.Based on the composition,microstructure,and surface morphology of PU@SiR-GO composites before and after combustion tests,a possible flame-retardance mechanism is proposed.This work provides a simple and effective strategy for fabricating flame retardant composites with improved mechanical performance. | Qian Wu Jincheng Zhang Shengpeng Wang Bajin Chen Yijun Feng Yongbing Pei Yue Yan Longcheng Tang Huayu Qiu Lianbin Wu | 2021 | Frontiers of Chemical Science and Engineering2021，15，4： | 1 |
| 13 | An approach to the Paal-Knorr pyrroles synthesis catalyzed by Sc (OTf) 3 under solvent-free conditions显示文摘 | CHEN JIUXI WU HUAYUE ZHENG ZHIGUO | | 0，，47： | 1 |
| 14 | Fabrication of ZIF-8 membranes on dual-layer ZnO-PES/PES organic hollow fibers by in-situ crystallization显示文摘Compared to inorganic supports, polymeric supports can offer additional benefits, e.g., easier processing and cheaper. However, the organic surface has weak adhesion to the zeolitic imidazolate frameworks(ZIFs) membrane layer, which usually requires complex surface modification or seeding. Herein, we demonstrate that a dual-layer asymmetric polymer support prepared by a simple spinning process is a good candidate for the preparation of ZIF-8 membrane. The inner layer of the support is an organic hollow fiber(PES) with finger-like pores, and the outer layer is a ZnO-PES composite layer with finger-like pores also. The ZnO-PES composite layer is expected to contain uniform ZnO crystals in the polymer matrix, i.e., the ZnO particles in the skin layer of the support are not easy to fall off. Under the induction of ZnO particles in the outer layers, continuous ZIF-8 membranes can be prepared by single in-situ crystallization, showing good adhesion to the supports. The obtained ZIF-8 membranes show a H_(2) permeance of 8.7 × 10^(-8)mol·m^(-2)·s^(-1)·Pa^(-1) with a H_(2)/N_(2) ideal separation selectivity of 18.0. The design and preparation of this dual-layer polymer support is expected to promote the large-scale application of MOF membranes on polymer supports. | Zhengchi Yin Xiaoke Wu Yanwei Yang Huayu Zhang Wangtao Li Ruimin Zhu Qiancheng Zheng Zhengbao Wang | 2023 | Chinese Journal of Chemical Engineering2023，55，3： | 1 |
| 15 | Global desert variation under climatic impact during 1982-2020显示文摘Deserts are important landscapes on the earth and their variations have impacts on global climate through feedback processes.However,there is a limited understanding of the climatic controls on the spatial and temporal variations of global deserts.Here,we use climate reanalysis datasets,global land use/land cover(LULC)products and the CMIP6(Coupled Model Intercomparison Project)model outputs to calculate the changing of global deserts during 1982-2020,and estimate future spatial trends of global deserts.Our results show that mean annual global desert area over this period is 17.64×10^(6)km^(2),accounting for 12%of the terrestrial land.Desert areas decreased rapidly from the end of the 1980s to the 1990s in North Africa and Australia,followed by a slow expansion in early 21st century globally.Spatio-temporal variations of areas of arid climate are characterized by interdecadal fluctuations,and there are clear regional differences in dynamic s of the aridity index(AI,used here as a proxy for the area of drylands)and desert areas.Statistical analyses reveal that increased vegetation cover is directly related to the reduction of desert area,while potential evaporation,surface temperature and humidity are also significantly correlated with the desert area.The relationship between wind speed and desert dynamics varies regionally.The results of the CMIP6simulations suggest that global deserts will expand in the 21st century,albeit at different rates under the ssp245 and ssp585scenarios.Desert expansions are modelled to be greatest in Asia,Africa and Australia,while those of southern North Africa may reduce as their southern borders migrate northwards. | Yao CHEN Huayu LU Huijuan WU Jingjing WANG Nana LYU | 2023 | Science China Earth Sciences2023，66，5： | 0 |
| 16 | Facile generation of highly durable thiol-functionalized polyhedral oligomeric silsesquioxane based superhydrophobic melamine foam显示文摘In this study,a durable superhydrophobic/superoleophilic melamine foam was fabricated by a facile and rapid one-step thiol-ene click chemistry and Michael addition reaction,which demonstrated excellent robustness in oil/water separation.First,1H,1H,2H-perfluoro-1-hexene reacted with thiol-functionalized polyhedral oligomeric silsesquioxane via the thiol-ene click chemistry to obtain a fluorinated thiol-functionalized polyhedral oligomeric silsesquioxane solution.Subsequently,the melamine foam was immersed to the solution system to form nanoaggregates on the melamine foam surface by the Michael addition reaction in the presence of ultraviolet light.The micro/nano rough structure and low surface energy of the nanoaggregates layer endowed the pristine melamine foam with superhydrophobicity;the water contact angle was greater than 150°.More importantly,the as-prepared melamine foam could withstand harsh conditions,such as a corrosive solution environment,strong ultraviolet light,mechanical compression,high and low temperature exposure,and ultrasonic washing.Driven by gravity,the as-prepared melamine foam could efficiently separate the oil/water mixtures and maintain 98%separation efficiency at high and low temperatures.In addition,it maintained the desirable absorption capability in different oil/organic solvents even after 15 absorption cycles.Accordingly,this facile,low-cost,and robust onestep method provides important support for the superhydrophobic oil/water separation field. | Meng Li Yuanfeng Fang Chun Liu Mengmeng Zhou Xiaomei Miao Yongbing Pei Yue Yan Wenjun Xiao Huayu Qiu Lianbin Wu | 2022 | Frontiers of Chemical Science and Engineering2022，16，8： | 0 |
| 17 | 8-Oxoguanine DNA glycosylase protects cells from senescence via the p53-p21 pathway显示文摘Cellular senescence is an important factor leading to pulmonary fibrosis.Deficiency of 8-oxoguanine DNA glyco-sylase(OGG1)in mice leads to alleviation of bleomycin(BLM)-induced mouse pulmonary fibrosis,and inhibition of the OGG1 enzyme reduces the epithelial mesenchymal transition(EMT)in lung cells.In the present study,we find decreased expression of OGG1 in aged mice and BLM-induced cell senescence.In addition,a decrease in OGG1 expression results in cell senescence,such as increases in the percentage of SA-β-gal-positive cells,and in the p21 and p-H2AX protein levels in response to BLM in lung cells.Furthermore,OGG1 promotes cell transformation in A549 cells in the presence of BLM.We also find that OGG1 siRNA impedes cell cycle progression and inhibits the levels of telomerase reverse transcriptase(TERT)and LaminB1 in BLM-treated lung cells.The increase in OGG1 expression results in the opposite phenomenon.The mRNA levels of senescence-associated secretory phenotype(SASP)components,including IL-1α,IL-1β,IL-6,IL-8,CXCL1/CXCL2,and MMP-3,in the absence of OGG1 are obviously increased in A549 cells treated with BLM.Interestingly,we demonstrate that OGG1 binds to p53 to inhibit the activation of p53 and that silencing of p53 reverses the inhibition of OGG1 on senescence in lung cells.Ad-ditionally,the augmented cell senescence is shown in vivo in OGG1-deficient mice.Overall,we provide direct evidence in vivo and in vitro that OGG1 plays an important role in protecting tissue cells against aging associated withthep53pathway. | Shenglan Gao Lujun Chen Ziying Lin Zhiliang Xu Yahong Wang Huayu Ling Zijun Wu Yu Yin Weimin Yao Keng Wu Gang Liu | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 18 | Two flowers per seed:Derivatives of CoG@F127/GO with enhanced catalytic performance of overall water splitting显示文摘In this work,cobalt glycerate(CoG@F127)nanosheets grown on the surface of graphene oxide(GO),i.e.CoG@F127/GO,have been synthesized with the assistance of nonionic surfactant Pluronic F127 via a hydrothermal method.After calcination,CoG@F127/GO is transformed into one derivative,Co nanoparticles coated with a trace amount of carbon(Co-C)on GO(Co-C/GO).The Co nanoparticles consist of an atypical core-shell structure,in which the core and the shell are both Co.Co-C anchored on GO can avoid the nanoparticles aggregation and expose more active sites for hydrogen evolution reaction(HER)to significantly improve the catalyst activity of HER.CoG@F127/GO is phosphatized to form the other derivate,cobalt pyrophosphate coated with a small amount of carbon(Co_(2)P_(2)O_(7)-C)on GO(Co_(2)P_(2)O_(7)-C/GO).Co_(2)P_(2)O_(7)-C/GO composite owns a large electrochemical active surface area(ECSA)and fast rate towards oxygen evolution reaction(OER).Furthermore,the two derivatives of CoG@F127/GO,i.e.Co-C/GO and Co_(2)P_(2)O_(7)-C/GO as twin flowers,are assembled into an overall water splitting electrolytic cell with a cell voltage of 1.56 V to deliver a current density of 10 mA cm^(-2). | Yue Han Chen Qian Huayu Wu Xing Chen Xue Wu Wei He Hui Yan Guisheng Li Guowang Diao Ming Chen | 2021 | Journal of Energy Chemistry2021，30，3： | 0 |
| 19 | Quasi‑Solid Electrolyte Interphase Boosting Charge and Mass Transfer for Dendrite‑Free Zinc Battery显示文摘The practical applications of zinc metal batteries are plagued by the dendritic propagation of its metal anodes due to the limited transfer rate of charge and mass at the electrode/electrolyte interphase.To enhance the reversibility of Zn metal,a quasi-solid interphase composed by defective metal-organic framework(MOF)nanoparticles(D-UiO-66)and two kinds of zinc salts electrolytes is fabricated on the Zn surface served as a zinc ions reservoir.Particularly,anions in the aqueous electrolytes could be spontaneously anchored onto the Lewis acidic sites in defective MOF channels.With the synergistic effect between the MOF channels and the anchored anions,Zn^(2+)transport is prompted significantly.Simultaneously,such quasi-solid interphase boost charge and mass transfer of Zn^(2+),leading to a high zinc transference number,good ionic conductivity,and high Zn^(2+)concentration near the anode,which mitigates Zn dendrite growth obviously.Encouragingly,unprecedented average coulombic efficiency of 99.8%is achieved in the Zn||Cu cell with the proposed quasi-solid interphase.The cycling performance of D-UiO-66@Zn||MnO_(2)(~92.9%capacity retention after 2000 cycles)and D-UiO-66@Zn||NH_(4)V_(4)O_(10)(~84.0%capacity retention after 800 cycles)prove the feasibility of the quasi-solid interphase. | Xueer Xu Yifei Xu Jingtong Zhang Yu Zhong Zhongxu Li Huayu Qiu Hao Bin Wu Jie Wang Xiuli Wang Changdong Gu Jiangping Tu | 2023 | Nano-Micro Letters2023，15，4： | 0 |
| 20 | CD47KO/CRT dual-bioengineered cell membrane-coated nanovaccine combined with anti-PD-L1 antibody for boosting tumor immunotherapy显示文摘Tumor vaccines trigger tumor-specific immune responses to prevent or treat tumors by activating the hosts’immune systems,and therefore,these vaccines have potential clinical applications.However,the low immunogenicity of the tumor antigen itself and the low efficiency of the vaccine delivery system hinder the efficacy of tumor vaccines that cannot produce high-efficiency and long-lasting antitumor immune effects.Here,we constructed a nanovaccine by integrating CD47KO/CRT dual-bioengineered B16F10 cancer cell membranes and the unmethylated cytosine-phosphate-guanine(CpG)adjuvant.Hyperbranched PEI25k was used to load unmethylated cytosine-phosphate-guanine(CpG)through electrostatic adsorption to prepare PEI25k/CpG nanoparticles(PEI25k/CpG-NPs).CD47KO/CRT dual-bioengineered cells were obtained by CRISPR-Cas9 gene editing technology,followed by the cell surface translocation of calreticulin(CRT)to induce immunogenic cell death(ICD)in vitro.Finally,the extracted cell membranes were coextruded with PEI25k/CpG-NPs to construct the CD47KO/CRT dual-bioengineered cancer cell membrane-coated nanoparticles(DBE@CCNPs).DBE@CCNPs could promote endocytosis of antigens and adjuvants in murine bone marrow derived dendritic cells(BMDCs)and induce their maturation and antigen cross-presentation.To avoid immune checkpoint molecule-induced T cell dysfunction,the immune checkpoint inhibitor,the anti-PD-L1 antibody,was introduced to boost tumor immunotherapy through a combination with the DBE@CCNPs nanovaccine.This combination therapy strategy can significantly alleviate tumor growth and may open up a potential strategy for clinical tumor immunotherapy. | Shengyang Liu Jiayan Wu Yuanji Feng Xiaoya Guo Tong Li Meng Meng Jie Chen Daquan Chen Huayu Tian | 2023 | Bioactive Materials2023，，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员