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| 1 | Identification of selenocyst-eine insertion sequence(SECIS) element in eukaryotic selenoproteins by RNA Draw program显示文摘The computer program RNA Draw was used to identify the secondary structures in the 3’untranslated regions (3’UTRs) of the mRNAs from 46 eukaryotic seleno-proteins among 7 species. The program found one or two possible SECIS elements in these selenoproteins. The SECIS element consists of a stem-loop or hairpin structure with three conserved sequences of AUGA-(A)AA-GA. SECIS element was not found by the RNA Draw program in randomly selected non-selenoproteins. The results showed that SECIS element is the unique character of the genes ofeukaryotic selenoproteins. Thus it is possible to use RNA Draw to search the SECIS elements in gene bank for potential new selenoproteins. | XU Huibi HUANG Kaixun QU Xianghu GAO Zhonghong LIU Qiong CHEN Runsheng XUAN Zhenyu | 2001 | Chinese Science Bulletin2001，46，14： | 2 |
| 2 | Influence of alloxan-induced diabetes and selenite treatment on blood glucose and glutathione levels in mice显示文摘 | SHENG Xiqun HUANG Kaixun Xu Huibi | 2005 | J Trace Elem Med Biol2005，18，3： | 1 |
| 3 | Protective effect of polysaccharide from the loach on the in vitro and in vivo peroxidative damage of hepatocyte显示文摘 | Chuanguang Qin Kaixun Huang Huibi Xu | 2002 | The Journal of Nutritional Biochemistry2002，，10： | 1 |
| 4 | Isolation andcharacterization of an ovel polysaceha-ride from the mueus of theloach, Misgumusan guillicandatus显示文摘 | QIN Chuanguang HUANG Kaixun XU Huibi | 2002 | Carbohydrate Polymers2002，49，： | 1 |
| 5 | Protective effects of flavonoids in the roots of scutellaria baicalensis georgi against hydrogen peroxide-induced oxidative stress in hs-sy5y cells显示文摘 | Zhonghong Gao Kaixun Huang Huibi Xu | 2001 | Pharmacological Research2001，，2： | 1 |
| 6 | Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi显示文摘 | Zhonghong Gao Kaixun Huang Xiangliang Yang Huibi Xu | 1999 | BBA - General Subjects1999，，3： | 1 |
| 7 | Isolation and characterization of a novel polysaccharide from the mucus of the loach, Misgurnus anguillicaudatus显示文摘 | Qin Chuangguang Huang Kaixun Xu Huibi | 2002 | Carbohydrate Polymers2002，49，： | 1 |
| 8 | Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi显示文摘 | Zhonghong Gao Kaixun Huang Xiangliang Yang Huibi Xu | 1999 | BBA - General Subjects1999，，3： | 1 |
| 9 | Protective Effects of Flavonoids in the Roots of Scutellaria Baicalensis Georgi aginst Hydrogen Peroxide Induced Oxidative Stress in HS - SY5Y Cells 显示文摘 | Kaixun Huang and Huibi Xu | 2001 | Pharmacological Research2001，43，2： | 1 |
| 10 | Toxicity and penetration of TiO 2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposure显示文摘 | Jianhong Wu Wei Liu Chenbing Xue Shunchang Zhou Fengli Lan Lei Bi Huibi Xu Xiangliang Yang Fan-Dian Zeng | 2009 | Toxicology Letters2009，，1： | 1 |
| 11 | Modification of Fe_3O_4 Magnetic Nanoparticles by L-dopa or Dopamine as an Enzyme Support显示文摘Fe_3O_4 magnetic nanoparticles were prepared by co-precipitation of Fe2+ and Fe3+ in an ammonia solution,and its size was about 36 nm measured by an atomic force microscope.Fe3O4 magnetic nanoparticles were modified by L-dopa or dopamine using sonication method.The analysis of FTIR clearly indicated the formation of Fe-O-C bond.Direct immobilization of trypsin(EC:3.4.21.4)on Fe3O4 magnetic nanoparticles with L-dopa and dopamine spacer was investigated using glutaraldehyde as a coupling agent.No significant changes in the size and magnetic property of the three kinds of magnetic nanoparticles linked with or without trypsin were observed.The existence of the spacer molecule on magnetic nanoparticles could greatly improve the activity and the storage stability of bound trypsin through increasing the flexibility of enzyme and changing the microenvironment on nanoparticles surface compared to the naked magnetic nanoparticles. | PENG Hong ZHANG Xiao HUANG Kaixun XU Huibi | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，4： | 1 |
| 12 | Protective effect of polysaccharide from the loach on the in vitro and in vivo peroxidative damage of hepatocyte显示文摘 | Chuanguang Qin Kaixun Huang Huibi Xu | 2002 | The Journal of Nutritional Biochemistry2002，13，10： | 1 |
| 13 | In silico identification of silkworm selenoproteomes显示文摘Selenium (Se) is an essential trace element in vivo. Its biological function is mainly exerted through selenoproteins. Selenocysteine (Sec), the active site of selenoproteins, is incorporated into the protein at an in-frame TGA codon under the guidance of Sec insertion sequence (SECIS) element in the 3′-untranslated region (UTR) of the gene. In this work, a method was developed and a series of programs were edited by PERL language to in silico identify selenoproteomes from the genome of domesticated silkworm (Bombyx mori). Out of 18510 annotated genes, 6348 was terminated with TGA codons, 249 containing both in-frame TGAs and SECIS elements in the 3′-UTRs. Alignments of those selenoprotein candidates with their cysteine (Cys)-containing homologs revealed that 52 genes had TGA/Cys pairs and similar flanking regions around the in-frame TGAs. Restricted by the patterns of SECIS elements only 5 genes were screened out to fully meet the requirements for selenoproteins. Among them glutathione S-transferase (GST) has been reported as a microbial selenoprotein, the other four are novel selenoproteins annotated as CG6024, CG5195, ATP-binding cassette transporter subfamily A (ABCA), and nuclear VCP-like protein. Derived from the general properties of GST, ABCA and VCP, silkworm selenoproteins may play important roles in redox regulation, Se storage and transportation, as well as cell apoptosis. | CHEN Ping DUAN Jun JIANG Liang LIU Qiong ZHAO Ping XIA Qingyou XU Huibi | 2006 | Chinese Science Bulletin2006，51，23： | 1 |
| 14 | Mechanisms of peroxynitrite-induced [Ca^(2+)]_i increase in single neuronal cell显示文摘The mechanism of peroxynitrite (ONOO -)induced [Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura2 microfluorometric technique. The results show that ONOO - caused a rapid increase of [Ca 2+ ] i when ONOO - was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (CdCl 2, Nifedipine) greatly inhibited the [Ca 2+ ] i increase induced by ONOO -, suggesting that the opening of LCa 2+ channel makes a great contribution to the [Ca 2+ ]\-i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO -induced [Ca 2+ ]\-i increase suggests that ONOO -activating LCa 2+ channel is partly related to its oxidative speciality. | YI Yong 1, CAI Dong 1, GAO Zhonghong 1, YANG Xiangliang 1, QU Anlian 1, XU Huibi 1* , WANG Xiaomin 2 and HAN Songping 3 1. Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, China 2. Neuros | 1998 | Chinese Science Bulletin1998，43，20： | 1 |
| 15 | Investigation with ESR on the Peroxy Radical Scavenging Effect of Selenium显示文摘 | Zhang Luoping Xu Huibi Liu Zhengxian | | 0，，： | 1 |
| 16 | Protective effects of flavonoids in the roots of SBG,against hydrogen peroxide-induced oxidative stress in HS-SYSY cells显示文摘 | Gao Zhonghong Huang Kaixun Xu Huibi | 2001 | Pharmacological Research2001，43，2： | 1 |
| 17 | The mechanism for the effect of selenium supplementation on immunity显示文摘 | Enjie Sun Huibi Xu Qiong Liu Jingyan Zhou Ping Zuo Junjian Wang | 1995 | Biological Trace Element Research1995，，3： | 1 |
| 18 | Antioxidant status and mineral contents in tissues of rutin and baiealin fed rats 显示文摘 | Zhonghong Gao Huibi Xu Xiaojun Chen | 2003 | Life Sciences2003，73，： | 1 |
| 19 | Probing the cytotoxicity of CdSe quantum dots with surface modification显示文摘 | Guoning Guo Wei Liu Jiangong Liang Zhike He Huibi Xu Xiangliang Yang | 2006 | Materials Letters2006，，8： | 1 |
| 20 | Isolation and characterization of a novel polysaccharide from the mucus of the loach, Misgurnus anguillicaudatus 显示文摘 | Qin Chuanguang Huang Kaixun Xu Huibi | 2002 | Carbohydrate Polymers2002，49，： | 1 |
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