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| 1 | Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase显示文摘AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small intestine.METHODS:For this study,primary culture of ICCs was prepared from the small intestine of the mouse.LPS was treated to the cells prior to measurement of the membrane currents by using whole-cell patch clamp technique.Immunocytochemistry was used to examine the expression of the proteins in ICCs.RESULTS:LPS suppressed the pacemaker currents of ICCs and this could be blocked by AH6809,a prostaglandin E2-EP2 receptor antagonist or NG-Nitro-Larginine Methyl Ester,an inhibitor of nitric oxide(NO) synthase.Toll-like receptor 4,inducible NO synthase or cyclooxygenase-2 immunoreactivity by specific antibodies was detected on ICCs.Catalase(antioxidant agent) had no action on LPS-induced action in ICCs.LPS actions were blocked by nuclear factor kB(NF-kB) inhibitor,actinomycin D(a gene transcription inhibitor),PD 98059(a p42/44 mitogen-activated protein kinases inhibitor) or SB 203580 [a p38 mitogen-activated protein kinases(MAPK) inhibitor].SB 203580 also blocked the prostaglandin E2-induced action on pacemaker currents in ICCs but not NO.CONCLUSION:LPS inhibit the pacemaker currents in ICCs via prostaglandin E2-and NO-dependent mechanism through toll-like receptor 4 and suggest that MAPK and NF-kB are implicated in these actions. | Dong Chuan Zuo Seok Choi Pawan Kumar Shahi Man Yoo Kim Chan Guk Park Young Dae Kim Jun Lee In Yeoup Chang Insuk So Jae Yeoul Jun | 2013 | World Journal of Gastroenterology2013，19，8： | 9 |
| 2 | Identification of TRPM7 channels in human intestinal interstitial cells of Cajal显示文摘AIM:To investigate the characteristics of slow elec-trical waves and the presence of transient receptor potential melastatin-type 7(TRPM7)in the human gas-trointestinal(GI)tract.METHODS:Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue.Immunohisto-chemistry was used to identify TRPM7 channels in in-terstitial cells of Cajal(ICCs).RESULTS:The human GI tract generated slow electri-cal waves and had ICCs which functioned as pacemak-er cells.Flufenamic acid,a nonselective cation channel blocker,and 2-APB(2-aminoethoxydiphenyl borate)and La3+,TRPM7 channel blockers,inhibited the slowwaves.Also,TRPM7 channels were expressed in ICCs in human tissue.CONCLUSION:These results suggest that the human GI tract generates slow waves and that TRPM7 chan-nels expressed in the ICCs may be involved in the gen-eration of the slow waves. | Byung Joo Kim Kyu Joo Park Hyung Woo Kim Seok Choi Jae Yeoul Jun In Youb Chang Ju-Hong Jeon Insuk So Seon Jeong Kim | 2009 | World Journal of Gastroenterology2009，15，46： | 3 |
| 3 | Inhibition of pacemaker currents by nitric oxide via activation of ATP-sensitive K+ channels in cultured interstitial cells of Cajal from the mouse small intestine显示文摘 | Chan Guk Park Young Dae Kim Man Yoo Kim Jun Soo Kim Seok Choi Cheol Ho Yeum Shankar Prasad Parajuli Jong Seong Park Han Seong Jeong Insuk So Ki Whan Kim Jae Yeoul Jun | 2007 | Naunyn - Schmiedeberg’s Archives of Pharmacology2007，，3： | 1 |
| 4 | Volume-sensitive chloride current activated by hyposmotic swelling in antral gastric myocytes of the guinea-pig显示文摘 | Wen Xie Xu Sung Joon Kim Insuk So Tong Mook Kang Jong Chul Rhee K. W. Kim | 1997 | Pflügers Archiv1997，，1： | 1 |
| 5 | Effect of stretch on calcium channel currents recorded from the antral circular myocytes of guinea-pig stomach显示文摘 | Wen Xie Xu Sung Joon Kim Sang Jeong Kim Insuk So Tong Mook Kang Jong Chul Rhee Ki Whan Kim | 1996 | Pflügers Archiv European Journal of Physiology1996，，2： | 1 |
| 6 | Reciprocal positive regulation between TRPV6 and NUMB in PTEN-deficient prostate cancer cells显示文摘 | Sung-Young Kim Chansik Hong Jinhong Wie Euiyong Kim Byung Joo Kim Kotdaji Ha Nam-Hyuk Cho In-Gyu Kim Ju-Hong Jeon Insuk So | 2014 | Biochemical and Biophysical Research Communications2014，，： | 1 |
| 7 | Effects of prostaglandin F_(2α) on small intestinal interstitial cells of Cajal显示文摘AIM:To explore the role of prostaglandin F2α(PGF2α) on pacemaker activity in interstitial cells of Cajal(ICC)from mouse small intestine. METHODS:In this study,effects of PGF2αin the cultured ICC cells were investigated with patch clamp technology combined with Ca 2+ image analysis. RESULTS:Externally applied PGF2α(10μmol/L)produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp mode.The application of flufenamic acid(a non-selective cation channel inhibitor)or niflumic acid(aCl channel inhibitor)abolished the generation of pacemaker currents but only flufenamic acid inhibited the PGF2α-induced tonic inward currents.In addition,the tonic inward currents induced by PGF2αwere not inhibited by intracellular application of 5’-[-thio]diphosphate trilithium salt.Pretreatment with Ca 2+ free solution, U-73122,an active phospholipase C inhibitor,and thapsigargin,a Ca 2+ -ATPase inhibitor in endoplasmic reticulum,abolished the generation of pacemaker currents and suppressed the PGF2α-induced tonic inward currents.However,chelerythrine or calphostin C,protein kinase C inhibitors,did not block the PGF2α-induced effects on pacemaker currents.When recording intracellular Ca 2+ ([Ca 2+ ]i)concentration using fluo-3/AM,PGF2α broadly increased the spontaneous[Ca 2+ ]i oscillations. CONCLUSION:These results suggest that PGF2αcan modulate pacemaker activity of ICC by acting non-selective action channels through phospholipase C-dependent pathway via[Ca2+]i | Chan Guk Park Young Dae Kim Man Yoo Kim Jae Woong Koh Jae Yeoul Jun Cheol Ho Yeum Insuk So Seok Choi | 2011 | World Journal of Gastroenterology2011，17，9： | 1 |
| 8 | Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity显示文摘瞄准:Cajal (国际计算中心) 的空隙的房间是产生慢波浪在的心律调整器房间胃肠(官方补给) 道。我们试图在 Cajal 的有教养的空隙的房间在肠的步调调整的活动调查 mitochondrial Na+-Ca2+ 交换的参与。方法:酶的消化被用来声言不赞成一只老鼠的小肠。整个房间的斑夹钳配置被用来记录膜电流(电压 clamp ) 和潜力(电流夹钳) 从有教养的国际计算中心。结果:Clonazepam 和 CGP37157 以一种剂量依赖者方式禁止了国际计算中心的步调调整的活动。从 20 ~ 60 micromol/L 的 Clonazepam 和从 10 ~ 30 micromol/L 的 CGP37157 有效地在国际计算中心的步调调整的活动从线粒体禁止了 Ca2+ 流出。clonazepam 和 CGP37157 的 IC50s 分别地是 37.1 和 18.2 micromol/L。到内部答案的 20 micromol/L NiCl2 的增加引起了一“打蜡”并且衰退国际计算中心的步调调整的活动的现象。结论:这些结果建议 mitochondrial Na+-Ca2+ 交换在肠的步调调整的活动有一个重要角色。 | Byung Joo Kim Jae Yeoul Jun Insuk So Ki Whan Kim | 2006 | World Journal of Gastroenterology2006，12，5： | 1 |
| 9 | Melastatin-Type Transient Receptor Potential Channel 7 Is Required for Intestinal Pacemaking Activity显示文摘 | Byung Joo Kim Hyun–Ho Lim Dong Ki Yang Jae Yeoul Jun In Youb Chang Chul–Seung Park Insuk So Peter R. Stanfield Ki Whan Kim | 2005 | Gastroenterology2005，，5： | 1 |
| 10 | Poncirus trifoliate fruit modulates pacemaker activity in interstitial cells of Cajal from the murine small intestine显示文摘 | Byung Joo Kim Hyung Woo Kim Guem San Lee Seok Choi Jae Yeoul Jun Insuk So Seon Jeong Kim | 2013 | Journal of Ethnopharmacology2013，，3： | 1 |
| 11 | Effects of Schisandra chinensis extract on gastrointestinal motility in mice显示文摘 | Tae Seok Ahn Dae Geon Kim Noo Ri Hong Hyun Soo Park Hyungwoo Kim Ki-Tae Ha Ju-Hong Jeon Insuk So Byung Joo Kim | 2015 | Journal of Ethnopharmacology2015，，： | 1 |
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