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| 1 | 应用轻便型显微镜视屏手术方法复制脑梗塞动物模型显示文摘目的为制备脑梗塞动物模型提供一种新颖、简便、廉价的方法.方法分别采用显微镜镜头手术技术(A组)和视屏手术技术(B组)阻断大脑中动脉,术中监测局部脑血流,术后第3天进行改良的神经症状严重程度记分、采用2,3,5-三苯-2H-四氮杂茂氯化物染色脑梗塞大鼠的脑片并计算脑梗塞灶的容积百分比.结果采用轻便型显微镜视屏手术技术阻断大脑中动脉,操作准确、平稳、微创、舒适;手术成功率可达74.07%(54%~89%);脑梗塞灶的容积百分比为15.22%(7.47%~22.97%).统计分析表明,A、B组的成功率没有显著性差异、B组内个体之间脑梗塞灶的容积百分比差异无统计学意义(P>0.05).结论采用轻便型显微镜视屏手术模式可成功地复制脑梗塞动物模型,其方法简便、廉价,微创、舒适. | 朱兴宝 张绎 Ivan Ng 刘松 李栋平 范泉水 | 2009 | 昆明医学院学报2009，30，12： | 15 |
| 2 | 轴突生长抑制因子DNA疫苗对大鼠中枢神经系统的影响(英文)显示文摘目的探讨轴突生长抑制因子DNA疫苗(pcDNA-NGIs)免疫是否会对中枢神经系统造成损害。方法以pcDNA-NGIs免疫成年Sprague-Dawley大鼠,免疫期间进行实验性自动免疫性脑髓鞘炎评分,同时观察实验动物的感觉运动功能和认知行为。结果以pcDNA-NGIs免疫的大鼠未出现实验性自动免疫性脑髓鞘炎症状。复合神经病学评分、粘附纸条清除试验和开放场地试验表明,pcDNA-NGIs免疫不会损害实验动物的感觉运动功能。另外,被动回避试验和主动回避试验证明,pcDNA-NGIs免疫不会引起学习和记忆障碍。结论pcDNA-NGIs免疫不会损害实验大鼠中枢神经系统。 | 朱兴宝 Jasmine Lee Jill Wong Wan Loo Tan 冯忠堂 肖志成 Ivan Ng | 2007 | 中华神经外科疾病研究杂志2007，6，3： | 3 |
| 3 | Poststroke DNA immunization against neurite growth inhibitors is beneficial to the recovery from local cerebral ischemia in rats显示文摘BACKGROUND: Inhibitory signals, i.e. neurite growth inhibitors (NGIs), presenting on central nervous system (CNS) myelin have been shown to play a crucial role in inhibiting lesioned axonal sprouting and leading to less functional recovery. Vaccines targeting NGIs may provide multifactorial protection against brain insults by overcoming the inhibitory effects of these NGIs and boosting the body's immune repair mechanisms. OBJECTIVE: To evaluate the effect of poststroke DNA immunization against NGIs on the rehabilitation for sensorimotor function of rat models of local cerebral ischemia. DESIGN: Completely randomized grouping design, and controlled experiment. SETTING: Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore. MATERIALS: Sixty adult male Sprague-Dawley rats ranging in age from 45 to 120 days and in weight from 180 to 250 grams were provided by Animal Center of Department of Anatomy, Faculty of Medicine, National University of Singapore. pcDNA3.1(+)-neurite growth inhibitors (pcDNA-NGIs) a gift was provided by Dr. Xiao from Department of Clinical Research, Singapore General Hospital, Singapore. METHODS: The experiment was carried out at Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore from August 2003 to April 2005. ①The involved rats were randomized into 3 groups: pcDNA-NGIs group (group A), pcDNA3.1 (+) group (group B) and model group (group C), with 20 rats in each group. Left focal cerebral ischemia (FCI) was permanently induced through middle cerebral artery occlusion (MCAO) with the assistance of an operating microscope. Successful MCAO was determined by a 20% decrease to baseline in the ipsilateral cerebral blood flow. 100 μg of pcDNA-NGIs eluted in phosphate-buffered saline (PBS) was intramuscularly injected into the tibial muscle once a week after MCAO for 6 weeks in group A. As control, pcDNA3.1 (+) was also administrated in the same way in group B and nothing was administrated in group C. ② The modified neurological severity score (mNSS), a composite of motor, sensory, reflex and balance tests, was used to test the sensorimotor deficit. The mNSS was graded on a scale of 0–18, i.e. normal score was 0, maximal deficit score was 18, and 1 point was warded for the inability to perform the tasks or the lack of a tested reflex. ③ The newly generated axons of corticorubral projection were traced by stereotaxic guided injection of 100 g/L biotinylated dextran amine. Rats were sacrificed two weeks after tracing, and cryostat coronal sections of midbrains (30 μm) were reacted to BDA according to the manufacturer's instruction by the free-floating method. Images were captured on a DM RXA2 LEICA Microscope with a Spot Digital Camera system (Germany), and the numbers of labeled axons on the denervated side in four standard coronal sections including the red nucleus were manually quantified. MAIN OUTCOME MEASURES: ① The number of newly generated axons of corticorubral projection. ②The improvement in sensorimotor deficit. RESULTS: All the involved 60 rats entered the stage of final analysis. ① The number of newly generated axons of corticorubral projection of rats: Only ipsilateral axons of CRP were noted with little evidence of fibers crossing to the contralateral red nucleus in rats of groups B and C. More BDA-positive fibers crossing the midline and terminating in the contralateral red nucleus in appropriate target areas mirroring thenon-differentiated red nucleus were found in rats of group A. Quantitative analysis showed that BDA-labeled axons in the denervated side of rats in group A were more than those in group B (P < 0.05). ② Improvement in sensorimotor deficit of rats: At 2 weeks after immunization, significant improvement in sensorimotor deficit was found in rats of group A. There were significant differences of improvement in sensorimotor deficit of rats between group A and group B or group C at 12 and 14 weeks after immunization (P < 0.05). CONCLUSION: ① Poststroke DNA immunization against NGIs leads to increased sensorimotor recovery following FCI and compensatory newly growth of axons from corticorubral projection. | Xingbao Zhu Jasmine Lee Jill Wong Wan Loo Tan Zhongtang Feng Tinghua Wang Zhicheng Xiao Ivan Ng | 2007 | Neural Regeneration Research2007，2，2： | 3 |
| 4 | Apoptosis Occurs after Cerebral Contusions in Humans显示文摘 | Ng Ivan Yeo Tseng-Tsai Tang Wen-Ying | 2000 | Neurosurg2000，46，4： | 2 |
| 5 | 轴突生长抑制因子DNA疫苗免疫促进脑缺血后神经功能恢复显示文摘目的为进一步证实突生长抑制因子DNA疫苗免疫对局部脑缺血后神经功能恢复的促进作用.方法采用永久性阻断SD大鼠大脑中动脉的方法制备左侧局部脑缺血模型;在中风前或中风后经腓肠肌注射轴突生长抑制因子DNA疫苗免疫动物,每周1次,共6周;分别采用改良神经病严重程度记分、被动逃避试验和悬臂迷宫试验评价中风后运动功能、认知行为和焦虑样情感的恢复.结果中风前或中风后进行轴突生长抑制因子DNA疫苗免疫,局部脑缺血后都出现明显的运动恢复.认知行为和焦虑样情感障碍则没有明显改善.结论中风前或中风后给予轴突生长抑制因子DNA疫苗免疫可促进脑缺血后的运动功能恢复. | 朱兴宝 Jasmine LEE Jill WONG 肖志成 范泉水 Ivan NG | 2008 | 昆明医学院学报2008，29，1： | 1 |
| 6 | Hypoxia inducible factor-1α and expression of-vascular endothelial growth factor and its receptors in cerebral arteriovenous malformations显示文摘 | Ivan Ng Wan-Loo Tan Puay-Yong | 2005 | Journal of Clinical Neuroscience2005，12，7： | 1 |
| 7 | Impact of Graft Type on Outcome in Pediatric Liver Transplantation: A Report From Studies of Pediatric Liver Transplantation (SPLIT)显示文摘 | Ivan R. Diamond Annie Fecteau J Michael Millis Julian E. Losanoff Vicky Ng Ravinder Anand Changhong Song | 2007 | Annals of Surgery2007，，2： | 1 |
| 8 | Endoscopic assisted microneurosurgery for cer-ebral aneurysm显示文摘 | Wang E Ng PY Ivan Ng | 2003 | J Clinical Neuroscience2003，10，2： | 1 |
| 9 | Evaluation of Treatment Response in Patients with Metastatic Renal Cell Carcinoma: Role of State-of-the-Art Cross-Sectional Imaging显示文摘 | Venkata S. Katabathina Nathalie Lassau Ivan Pedrosa Chaan S. Ng Srinivasa R. Prasad | 2012 | Current Urology Reports2012，，1： | 1 |
| 10 | Endoscopic assisted microneurosurgery for cerebral aneurysm 显示文摘 | Wang E Ng PY Ivan Ng | 2003 | J Clinical Neuroscience2003，10，2： | 1 |
| 11 | Apoptosis Occurs after Cerebral Contusions in Humans显示文摘 | Yeo Tseng Tsai Tang Wen Ying | 2000 | Neurosurg2000，46，4： | 1 |
| 12 | Apoptosis occurs after cerebral contusions in humans显示文摘 | Ng Ivan Yeo TT Tang WY | 2000 | Neurosurgery2000，46，： | 1 |
| 13 | Contribution of Fibrosis and the Autonomic Nervous System to Atrial Fibrillation Electrograms in Heart Failure显示文摘 | Hemantha Koduri Jason Ng Ivan Cokic Gary L. Aistrup David Gordon J. Andrew Wasserstrom Alan H. Kadish Richard Lee Rod Passman Bradley P. Knight Jeffrey J. Goldberger Rishi Arora | 2012 | Circulation: Arrhythmia and Electrophysiology2012，，4： | 1 |
| 14 | Analysis of the Viability of an Urban Renewal Project under a Risk-Based Option Pricing Framework显示文摘 | Eddie Chi-man Hui Ivan Man-hon Ng Kak-keung Lo | 2011 | Journal of Urban Planning and Development2011，137，： | 1 |
| 15 | 中风前抗轴突生长抑制因子DNA免疫促进脑缺血后神经再生(英文)显示文摘目的为了澄清中风前抗轴突生长抑制因子DNA免疫对局部脑缺血神经再生的促进作用。方法经腓肠肌注射抗轴突生长抑制因子DNA疫苗免疫动物,每周一次,共6w;血清中检测出相关抗体后,采用永久性阻断大脑中动脉的方法制备左侧局部脑缺血模型,通过立体定向脑内注射生物素化葡聚糖胺(BDA)追踪皮质红核束的新生轴索。结果中风前接受抗轴突生长抑制因子DNA免疫,局部脑缺血后皮质红核束的代偿性新生轴索明显增多。结论中风前抗轴突生长抑制因子DNA免疫可提高永久性局部脑缺血后的神经再生。 | 朱兴宝 Jasmine Lee Jill Wong Wan Loo Tan 王廷华 冯忠堂 Xiao Zhicheng Ivan Ng | 2008 | 中华神经外科疾病研究杂志2008，7，1： | 1 |
| 16 | Intraoperative Computed Tomographic Angiography in Cerebral Aneurysm Surgery: A Pilot Feasibility Study显示文摘 | Shree Kumar Dinesh John Thomas Ivan Ng | 2010 | Neurosurgery2010，，2： | 1 |
| 17 | Apoptosis and traumatic brain injury显示文摘 | Jill Wong Ng Wai Hoe Feng Zhiwei Ivan Ng | 2005 | Neurocritical Care2005，，2： | 1 |
| 18 | Endoscopic assisted microneumsurgery for cerebral aneurysm 显示文摘 | ERNEST WANG NG PUAY YONG IVAN NG | 2003 | Journal of Clinical Neumscience2003，10，2： | 1 |
| 19 | 中风后抗轴突生长抑制因子DNA免疫促进脑缺血功能恢复显示文摘目的探讨中风后抗轴突生长抑制因子DNA免疫对脑缺血神经功能康复的促进作用。方法通过永久性阻断大脑中动脉诱导左侧局部脑缺血后,经腓肠肌注射抗轴突生长抑制因子DNA疫苗免疫动物,每周一次、共6周;分别采用改良的神经病严重程度记分、被动逃避试验和悬臂迷宫试验来评价运动功能、认知行为和焦虑样情感的恢复。结果中风后接受抗轴突生长抑制因子DNA免疫,局部脑缺血后出现明显的运动功能恢复,但是认知行为和焦虑样情感障碍则没有明显改善。结论中风后抗轴突生长抑制因子DNA免疫可促进脑缺血后的运动功能恢复。 | 朱兴宝 Jasmine Lee Jill Wong Wan Loo Tan 王廷华 冯忠堂 Xiao Zhicheng Ivan Ng | 2007 | 西南军医2007，9，4： | 0 |
| 20 | Eucleanal A and B:Two new napthalene derivatives from Euclea divinorum显示文摘二新萘衍生物把 eucleanal 称为 A, B (1 和 2 ) 从 Euclea divinorum 被孤立。新混合物的结构被象 1H 那样的详细分光镜的分析阐明, 13C NMR,舒适, HMQC, HMBC 和 HREIMS 集中 spectrometry。 | Margaret Mwihaki Ng'ang'a Hidayat Hussain Sumesh Chhabra Caroline Langat-Thoruwa Ahmed AI-Harrasi Karsten Krohn Ivan Robert Green | 2012 | Chinese Chemical Letters2012，23，5： | 0 |
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