| 3 | Experimental and Data Fitting Guidelines for the Determination of Polymer Crystallization Kinetics显示文摘The crystallization kinetics of semicrystalline polymers is often studied with isothermal experiments and analyzed by fitting the data with analytical expressions of the Avrami and Lauritzen and Hoffman(LH)theories.To correctly carry out the analysis,precautions in both experiments and data fitting should be taken.Here,we systematically discussed the factors that influence the validity of the crystallization kinetics study.The basic concepts and fundamentals of the Avrami and LH theories were introduced at first.Then,experimental protocols were discussed in detail.To clarify the impact of various experimental parameters,selected common polymers,i.e.,polypropylene and polylactide,were studied using various experimental techniques(i.e.,differential scanning calorimetry and polarized light optical microscopy).Common mistakes were simulated under conditions when non-ideal experimental parameters were applied.Furthermore,from a practical point of view,we show how to fit the experimental data to the Avrami and the LH theories,using an Origin■App developed by us. | Ricardo Arpad Perez-Camargo Guo-Ming Liu Du-Jin Wang Alejandro J.Muller | 2022 | Chinese Journal of Polymer Science2022，40，6： | 1 |
| 4 | An enhanced chemopreventive effect of methyl donor S-adenosylmethionine in combination with 25-hydroxyvitamin D in blocking mammary tumor growth and metastasis显示文摘Therapeutic targeting of metastatic breast cancer still remains a challenge as the tumor cells are highly heterogenous and exploit multiple pathways for their growth and metastatic spread that cannot always be targeted by a single-agent monotherapy regimen.Therefore,a rational approach through simultaneous targeting of several pathways may provide a better anti-cancer therapeutic effect We tested this hypothesis using a combination of two nutraceutical agents S-adenosylmethionine(SAM)and Vitamin D(Vit.D)prohormone[25-hydroxyvitamin D;/25(OH)D,]that are individually known to exert distinct changes in the expression of genes involved in tumor growth and metastasis.Our results show that both SAM and 25(OH)D monotherapy significantly reduced proliferation and clonogenic survival of a panel of breast cancer cell lines in vitro and inhibited tumor growth,lung metastasis,and breast tumor cell colonization to the skeleton in vivo.However,these effects were significantly more pronounced in the combination setting.RNA-Sequencing revealed that the transcriptomic footprint on key cancer-related signaling pathways is broader in the combination setting than any of the monotherapies.Furthermore,comparison of the differentially expressed genes from our transcriptome analyses with publicly available cancer-related dataset demonstrated that the combination treatment upregulates genes from immune-related pathways that are otherwise downregulated in bone metastasis in vivo.Since SAM and Vit.D are both approved nutraceuticals with known safety profiles,this combination treatment may serve as a novel strategy to reduce breast cancer-associated morbidity and mortality。 | Niaz Mahmood Ani Arakelian William J.Muller Moshe Szyf Shafaat A.Rabbani | 2020 | Bone Research2020，8，3： | 0 |
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