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| 1 | Effective vehicle detection technique for traffic surveillance systems显示文摘 | Ji Xiaopeng Wei Zhiqiang Feng Yewei | 2006 | Journal of Visual Communication and Image Representation2006，17，3： | 1 |
| 2 | Microgia and micreglia-like cell differentiated from DC inhibiet CD4 T cell proliferation 显示文摘 | Bo bai wengang song Yewei ji | 2009 | PLOS One2009，4，11： | 1 |
| 3 | Effective vehicle detection technique for traffic surveillance systems 显示文摘 | JI Xiaopeng WEI Zhiqiang FENG Yewei | 2006 | Journal of Visual Communication and Image Representation2006，17，3： | 1 |
| 4 | Effective vehicle detection technique for traffic surveillance systems 显示文摘 | Ji Xiaopeng Wei Zhiqiang Feng Yewei | 2006 | Journal of Visual Communication and Image Representation2006，17，3： | 1 |
| 5 | Microstructural formation and mechanical performance of friction stir double-riveting welded Al-Cu joints显示文摘A novel friction stir double-riveting welding(FSDRW) technology was proposed in order to realize the high-quality joining of upper aluminum(Al) and lower copper(Cu) plates,and this technology employed a Cu column as a rivet and a specially designed welding tool with a large concave-angle shoulder. The formations, interfacial characteristics, mechanical properties and fracture features of Al/Cu FSDRW joints under different rotational velocities and dwell times were investigated. The results showed that the well-formed FSDRW joint was successfully obtained.The cylindrical Cu column was transformed into a double riveting heads structure with a Cu anchor at the top and an Al anchor at the bottom, thereby providing an excellent mechanical interlocking.The defect-free Cu/Cu interface was formed at the lap interface due to the sufficient metallurgical bonding between the Cu column and the Cu plate, thereby effectively inhibiting the propagation of crack from the intermetallic compound layer at the lap interface between the Al and Cu plates. The tensile shear load of joint was increased first and then decreased when the rotational velocity and dwell time of welding tool increased, and the maximum value was 5.52 k N. The FSDRW joint presented a mixed mode of ductile and brittle fractures. | Shude JI Zhiqing ZHANG Peng GONG Hua LIU Xiao CUI Yewei ZHANG | 2023 | Chinese Journal of Aeronautics2023，36，8： | 0 |
| 6 | Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency显示文摘The purpose of this work was to construct the plasmid that could direct the synthesis of siRNA-like transcripts and thus mediate strong and specific repression of human heat shock protein 90β(Hsp90β)gene expression and to compare the transfection efficiency of the plasmids in varying conditions of transfection.Three 64 nt oligos corresponding to different regions of the target gene were chemically synthesized and annealed and were then ligated with pSUPER EGFP1 plasmid and double-digested with HindIII and BglII.Recombinant plasmids were transformed into Escherichia coli,DH5a,and the colonies were picked and grown in the Amp-agarose.The presence of positive clones was checked by the means of endodigestion and sequencing.Three cell strains,HepG2,Human umbilicus vein endothelium cells(HUVEC)and HeK293,were cultured.Then the plasmids were transfected into the cells at different ratios of plasmid to Lipofectamine.The transfection efficiency was measured by detection of enhanced green fluorescence protein(EGFP).The presence of positive recombinant clones were verified by double-digestion and sequencing.The bases inserted into the plasmids were correct and the positive colonies were named pSuper-Hsp90β1,pSuper-Hsp90β2 and pSuper-Hsp90β3.After optimizing the ratio of plasmid to Lipofectamine,we achieved high transfection efficiency in HeK293 cells.Transfection efficiency was still low in the HepG2 cells.In conclusion,the si-RNA-synthesizing plasmids targeting Hsp90βwere constructed and transfected into cells with different transfection efficiency. | JI Yewei NIE Bin LI Ping XU Xiaoyu ZHOU Yuanguo | 2007 | Frontiers of Medicine2007，1，3： | 0 |
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