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| 1 | Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide显示文摘Cytidine base editor(CBE),which is composed of a cytidine deaminase fused to Cas9 nickase,has been widely used to induce C-to-T conversions in a wide range of organisms.However,the targeting scope of current CBEs is largely restricted to protospacer adjacent motif(PAM)sequences containing G,T,or A bases.In this study,we developed a new base editor termed“nNme2-CBE”with excellent PAM compatibility for cytidine dinucleotide,significantly expanding the genome-targeting scope of CBEs.Using nNme2-CBE,targeted editing efficiencies of 29.0%-55.0%and 17.3%—52.5%were generated in human cells and rabbit embryos,respectively.In contrast to conventional nSp-CBE,the nNme2-CBE is a natural high-fidelity base editing platform with minimal DNA off-targeting detected in vivo.Significantly increased efficiency in GC context and precision were determined by combining nNme2Cas9 with rationally engineered cytidine deaminases.In addition,the Founder rabbits with accurate single-base substitutions at Fgf5 gene loci were successfully generated by using the nNme2-CBE system.These novel nNme2-CBEs with expanded PAM compatibility and high fidelity will expand the base editing toolset for efficient gene modification and therapeutic applications. | Zhiquan Liu Siyu Chen Yingqi Jia Huanhuan Shan Mao Chen Yuning Song Liangxue Lai Zhanjun Li | 2021 | Science China(Life Sciences)2021，64，8： | 2 |
| 2 | Systemic PPARgamma deletion impairs circadian rhythms of behavior and metabolism显示文摘 | YANG Guangrui JIA Zhanjun AOYAGI T | 2012 | PLoS One2012，7，38： | 1 |
| 3 | A pPeptide targeted contrast agent specific to fibrin-fibronectin complexes for cancer molecular imaging with MRI显示文摘 | Furong Ye Eun-Kee Jeong Zhanjun Jia | 2008 | Bioconjugate Chem2008，19，： | 1 |
| 4 | The mechanism study of glycosides of cistanches improv ing cognitive performance dysfunction in senescence accelerated mouse prone 8 by AMPK/mTOR signaling pathway显示文摘To investigate the mechanism of glycosides of cistanches improving cognitive performance dysfunction in SAMP8 mice by AMPK/mTOR signaling pathway.Male 7-month-old SAMP8 mice were randomly divided into 5 groups:model group,GCs group,GCs+3-MA group,GCs+compound C group,Vehicle group.Morris water maze was used to detect the ability of learning and memory.Western blot was used to detect the protein expression of SYN,PSD-95,BDNF,LC3,AMPK and m-TOR.The escape latency of GCs group,GCs+3-MA group and GCs+compound C group were significantly shortened(P<0.05).Compared with GCs group,the escape latency and percent of time spent in the target quadrant of GCs+compound C group were significantly increased(P<0.05). | Liu Xinlang Zhou Lili Sun Yihong Mo Chaoran Jia Jianxin Yang Zhanjun | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 5 | The neuroprotective efets of total flavones of Dracoce phalum moldavica L.on Parkinson's disease model in mouse显示文摘It was found that the extract of total flavones from Dracoce phalum moldavica L.(TFD)could exert neuroprotective effect on moase Parkinson's disease(PD)model,but the underlying mechanisms remained unclear.The aim of this study was to detect the mechanisms of TFD neuroprotective effect on PD mice.The PD model was established by injection with MPTP to mice.Using behavioral experiment and ELISA test as well as immunohistochemistry and Western blot analysis,the following parameters were examined:①the falling latency and climbing time,②the content of serum MDA as well as activities of SOD and GSH-Px,③the count of dopaminergic neurons with expression of TH in the substantia nigra of model mice,and④the expression levels of TH,caspase-3,Bcl-2 and Bax in the midbrain tissue. | Zhou Lili Mo Chaoran Liu Xinlang Sun yihong Jia Jianxin Yang Zhanjun | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 6 | The mechanism study of glycosides of cistanches improving cognitive performance dysfunction in senescence accelerated mouse prone 8 by AMPK/mTOR signaling pathway显示文摘To investigate the mechanism of glycosides of cistanches improving cognitive performance dysfunction,male 7-month-old SAMP8 mice were randomly divided into 5 groups:model group,GCs group,GCs+3-MA group,GCs+Compound C group,vehicle group.Morris water maze was used to detect the ability of learning and memory. | Liu Xinlang Zhou Lili Sun Yihong Mo Chaoran Jia Jianxin Yang Zhanjun | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 7 | Targeted mutagenesis in rabbit using an engineered BhCas12b variant显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeat(CRISPR)and CRISPR-associated protein(CRISPR–Cas)system has exhibited powerful abilities to manipulate genomes of animals and plants(Knott and Doudna,2018).Up to now,numerous Cas nucleases have been harnessed for genome editing in human cells,such as Cas9,Cas12a(also known as Cpf1),and Cas12b(also termed C2c1).Cas12b,a Class 2 type V-B CRISPR system,generates staggered double-strand breaks(DSBs)in the target DNA(Stella et al.,2017)and recognizes a distal 5′-T-rich protospacer adjacent motif(PAM)sequence(Shmakov et al.,2015),making it a complement to Cas9(recognizing 5′-NGG-3′PAM)in genome editing.Three Cas12b nucleases have been engineered for targeted genome editing in mammals or plants:BhCas12b v4(Strecker et al.,2019),BvCas12b(Strecker et al.,2019),and AaCas12b(Teng et al.,2018).However,they have not been compared parallelly with each other.In this study,we compared the three Cas12b proteins for genome editing in mammalian cells. | Yingqi Jia Tian Wang Ding Zhao Zhiquan Liu Tingting Sui Siyu Chen Jinze Li Liangxue Lai Zhanjun Li | 2022 | Journal of Molecular Cell Biology2022，14，12： | 0 |
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