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| 1 | Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells显示文摘Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy. | Jing Bai Jialun Duan Rui Liu Yafei Du Qian Luo Yinuo Cui Zhanbo Su Jiarui Xu Ying Xie Wanliang Lu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 5 |
| 2 | The Influence of Zusanli and Nonmeridian Acupuncture Points on the Survival Rate and Intestinal Tissue Features after Fatal Hemorrhagic Shock in Rats显示文摘 | Xian Shi Yuxian Zhong Jiarui Yao Sen Hu Lu Wang Gerhard Litscher Weibo Zhang | 2013 | Evidence-Based Complementary and Alternative Medicine2013，，： | 2 |
| 3 | RNA-binding protein QKI regulates contact inhibition via Yes-associate protein in ccRCC显示文摘Contact inhibition adjusts organ size to the proper size and ensures the cultured cells growing to a monolayer.By regulating the downstream coordinator YAP,the evolutionarily conserved Hippo transduction pathway attunes cell growth and death in response to cell contact inhibition,polarity,self-renewal,and differentiation.Dysregulation of this pathway is involved in various diseases such as cancer.RNA-binding protein QKI regulates cell proliferation,metabolism,division,and immunity in various cancer models,but its role in cancer cell contact inhibition remains unclear.In this study,we aimed to clarify the relationship between QKI and YAP,and the role of their interaction in cell contact inhibition.We found a lower QKI expression level in sparse condition,whereas a higher expression level in confluent condition by western blot analysis and immunofluorescence assay.QKI knockdown elevated cell proliferation and invasion both in vitro and in vivo.Strikingly,the results of CCK-8 assay,colony formation assay,and transwell assay showed that the phenomenon was in accord with the expression level of pYAP and reverse with YAP.Higher levels of Wnt3a and β-catenin were also found in xenografts of QKI-knockdown clear cell renal cell carcinoma (ccRCC) CAKI-1 cells by western blot analysis and immumohistochemical staining.Finally,a positive correlation between QKI and pYAP was found in clinical specimens by immunohistochemistry.Thus,as a negative regulator of YAP,QKI attuned the cell contact inhibition,leading to inhibition of cancer cell proliferation and invasion through Wnt and GPCR pathway. | Zheng Zhu Di Wei Xi'an Li Fuli Wang Fei Yan Zibao Xing Zhao Yan Huanyu Lu Dongsheng Zhai Ziehen Ye Geng Zhang Ping Meng Yu Zheng Jiarui Yuan Zifan Lu Jianlin Yuan | 2019 | Acta Biochimica et Biophysica Sinica2019，51，1： | 2 |
| 4 | Preparation of porous sea-urchin-like CuO/ZnO composite nanostructure consisting of numerous nanowires with improved gas-sensing performance显示文摘A sea-urchin-like CuO/ZnO porous nanostructure is obtained via a simple solution method followed by a calcination process.There are abundant pores among the resulting nanowires due to the thermal decomposition of copper-zinc hydroxide carbonate.The specific surface area of the as-prepared CuO/ZnO sample is determined as 31.3 m^(2)·g^(-1).The gas-sensing performance of the sea-urchin-like CuO/ZnO sensor is studied by exposure to volatile organic compound(VOC)vapors.With contrast to a pure porous sea-urchin-like ZnO sensor,the sea-urchin-like CuO/ZnO sensor shows superior gas-sensing behavior for acetone,formaldehyde,methanol,toluene,isopropanol and ethanol.It exhibits a high response of 52.6-100 ppm acetone vapor,with short response/recovery time.This superior sensing behavior is mainly ascribed to the porous nanowireassembled structure with abundant p-n heterojunctions. | Haibo REN Huaipeng WENG Pengfei ZHAO Ruzhong ZUO Xiaojing LU Jiarui HUANG | 2022 | Frontiers of Materials Science2022，16，1： | 1 |
| 5 | Efficacy of real-time PCR-based detection of Helicobacter pylori infection and genotypic resistance-guided quadruple therapy as the first-line treatment for functional dyspepsia with Helicobacter pylori infection显示文摘 | Qian Liu Dongdong Qi Jiarui Kang Yiduo Jin Wenxiang Liu Wenbin Gao Peng Hou Jiangyang Lu | 2015 | European Journal of Gastroenterology & Hepatology2015，，3： | 1 |
| 6 | Single-crystalline Mn-based oxide as a high-rate and long-life cathode material for potassium-ion battery显示文摘Mn-based oxides are promising cathode materials for potassium-ion batteries due to their high theoretical ca-pacity and abundant raw materials.However,the anisotropic properties of their conventional polycrystalline structures lead to insufficient rate capability and cycle life.Here,a single-crystal Mn-based layered oxide,P3′-type K_(0.35)Mn_(0.8)Fe_(0.1)Cu_(0.1)O_(2)(KMFCO),is designed and synthesized through a bimetallic co-induction effect and used as a cathode for potassium-ion battery.Benefiting from a unique single-crystal structure that is devoid of grain boundaries,it achieves a higher Kþtransport rate and a reduced volume change during the Kþintercalation/deintercalation process.Accordingly,the single-crystal P3′-type KMFCO delivers superior rate capability(52.9 mAh g^(-1) at 1000 mA g^(-1))and excellent cycling stability(91.1%capacity retention after 500 cycles at 500 mA g^(-1)).A full cell assembled with the P3′-type KMFCO cathode and a graphite anode also exhibits a high reversible capacity(81.2 mAh g^(-1) at 100 mA g^(-1))and excellent cycling performance(97%capacity retention after 300 cycles).The strategy of developing single-crystal materials may offer a new pathway for maintaining structural stability and improving the rate capability of layered manganese oxide cathodes and beyond. | Jiarui Lv Bin Wang Jiaxin Hao Hongbo Ding Ling Fan Renqian Tao Hongguan Yang Jiang Zhou Bingan Lu | 2023 | eScience2023，3，1： | 1 |
| 7 | Timing and Single-pulse Study of Pulsar J1909+0122 Discovered by CRAFTS显示文摘We report the discovery of PSR J1909+0122 by the Five-hundred-meter Aperture Spherical Radio Telescope(FAST)as part of the Commensal Radio Astronomy FAST Survey.PSR J1909+0122 has a spin period of 1.257 s and a dispersion measure of 186.2 pc cm^(-3).The averaged pulse profile shows two distinct components.We performed a single-pulse study based on a one-hour observation at 1.25 GHz on 2021 August 23.We used a threshold of 5σ_(ep) to measure the nulling fraction(NF)as 63%±1.5%.The longitude-resolved fluctuation spectra and fast Fourier transform spectra of the binary sequences revealed the quasi-periodicity of nulling with a period of 30 rotation periods.We examined the reliability of the periodicity by comparing it to random noise injection.The NF,E,and modulation periodicity P_(M) of PSR J1909+0122 were compared with other periodic nulling pulsars,showing that the source of J1909+0122 has the second largest NF in the population.Long-term timing observations over six months were used to derive the phase-connected ephemeris of this pulsar.The measured P and P values disfavor dipolar geometry for polar gap models,and the prediction for a space-charge-limited flow model in the case of inverse Compton scattering is only just above the death line.In this work,PSR J1909+0122 has revealed possible correlations between nulling behavior and pulsar properties,which will help to shed light on the pulsar emission mechanism and its temporal evolution in future observations. | Yutong Chen Pei Wang Di Li Erbil Gügercinoğlu Rushuang Zhao Lingqi Meng Jianping Yuan Jiarui Niu Weiwei Zhu Yi Feng Chenchen Miao Chenhui Niu Qingdong Wu Na Wang Shen Wang Xiaoyao Xie Mengyao Xue Jumei Yao Mao Yuan Shanping You Xuhong Yu Youling Yue Jie Zhang Junshuo Zhang Lei Zhang Yabiao Wang Zhenye Gan Yuxi Li Zhongyi Sun Chengjie Wang | 2023 | Research in Astronomy and Astrophysics2023，23，8： | 0 |
| 8 | Tuning Surface Spin Polarization of CoFeB by Boron Diffusion Detected by Spin Resolved Photoemission显示文摘Research of spin polarization of magnetic CoFeB thin films is of practical importance in spintronic applications.Here,using a direct characterization technique of spin-resolved photoemission spectroscopy,we obtain the surface spin polarization of amorphous Co_(40)Fe_(40)B_(20)thin films with different annealing temperatures from 100℃to 500℃prepared by magnetron sputtering.After high annealing temperature,a quasi-semiconductor state is gradually formed at the CoFeB surface due to the boron diffusion.While the global magnetization remains almost constant,the secondary electrons’spin polarization,average valence band spin polarization and the spin polarization at Fermi level from spin-resolved photoemission spectroscopy show a general trend of decreasing with the increasing annealing temperature above 100℃.These distinct surface properties are attributed to the enhanced Fe-B bonding due to the boron segregation upon surface after annealing as confirmed by x-ray photoelectron spectroscopy and scanning transmission electron microscopy with energy dispersive spectroscopy.Our findings provide insight into the surface spin-resolved electronic structure of the CoFeB thin films,which should be important for development of high-performance magnetic random-access memories. | Qi Liu Xianyang Lu Chengrui Fu Jiarui Chen Zhe Zhang Yuting Gong Xinyue Wang Yu Yan Qinwu Gao Hui Li Xuezhong Ruan Yao Li Jun Du Jing Wu Liang He Bo Liu Rong Zhang Yongbing Xu | 2023 | Chinese Physics Letters2023，40，8： | 0 |
| 9 | A multidimensional platform of patient-derived tumors identifies drug susceptibilities for clinical lenvatinib resistance显示文摘Lenvatinib,a second-generation multi-receptor tyrosine kinase inhibitor approved by the FDA for first-line treatment of advanced liver cancer,facing limitations due to drug resistance.Here,we applied a multidimensional,high-throughput screening platform comprising patient-derived resistant liver tumor cells(PDCs),organoids(PDOs),and xenografts(PDXs)to identify drug susceptibilities for conquering lenvatinib resistance in clinically relevant settings.Expansion and passaging of PDCs and PDOs from resistant patient liver tumors retained functional fidelity to lenvatinib treatment,expediting drug repurposing screens.Pharmacological screening identified romidepsin,YM155,apitolisib,NVP-TAE684 and dasatinib as potential antitumor agents in lenvatinib-resistant PDC and PDO models.Notably,romidepsin treatment enhanced antitumor response in syngeneic mouse models by triggering immunogenic tumor cell death and blocking the EGFR signaling pathway.A combination of romidepsin and immunotherapy achieved robust and synergistic antitumor effects against lenvatinib resistance in humanized immunocompetent PDX models.Collectively,our findings suggest that patient-derived liver cancer models effectively recapitulate lenvatinib resistance observed in clinical settings and expedite drug discovery for advanced liver cancer,providing a feasible multidimensional platform for personalized medicine. | Lei Sun Arabella H.Wan Shijia Yan Ruonian Liu Jiarui Li Zhuolong Zhou Ruirui Wu Dongshi Chen Xianzhang Bu Jingxing Ou Kai Li Xiongbin Lu Guohui Wan Zunfu Ke | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 10 | Programming the lifestyles of engineered bacteria for cancer therapy显示文摘Bacteria can be genetically engineered to act as therapeutic delivery vehicles in the treatment of tumors,killing cancer cells or activating the immune system.This is known as bacteria-mediated cancer therapy(BMCT).Tumor invasion,colonization and tumor regression are major biological events,which are directly associated with antitumor effects and are uncontrollable due to the influence of tumor microenvironments during the BMCT process.Here,we developed a genetic circuit for dynamically programming bacterial lifestyles(planktonic,biofilm or lysis),to precisely manipulate the process of bacterial adhesion,colonization and drug release in the BMCT process,via hierarchical modulation of the lighting power density of near-infrared(NIR)light.The deep tissue penetration of NIR offers us a modality for spatio-temporal and non-invasive control of bacterial genetic circuits in vivo.By combining computational modeling with a high-throughput characterization device,we optimized the genetic circuits in engineered bacteria to program the process of bacterial lifestyle transitions by altering the illumination scheme of NIR.Our results showed that programming intratumoral bacterial lifestyle transitions allows precise control of multiple key steps throughout the BMCT process and therapeutic efficacy can be greatly improved by controlling the localization and dosage of therapeutic agents via optimizing the illumination scheme. | Shengwei Fu Rongrong Zhang Yanmei Gao Jiarui Xiong Ye Li Lu Pu Aiguo Xia Fan Jin | 2023 | National Science Review2023，10，5： | 0 |
| 11 | Enhanced Corrosion Resistance and Biofilm Inhibition of Functionally Graded TC4/TC4-5Cu Fabricated by Selective Laser Melting against Streptococcus mutans显示文摘Ti-6Al-4V(TC4)used in dentistry and orthopedics as implant biomaterial faces the risk of microbiologically influenced corrosion(MIC)owing to the residence of diverse oral microorganisms.Hereinto,Streptococcus mutans is a critical pathogenic microorganism that causes dental caries.This work investigated the corrosive effects of S.mutans on TC4 and functional gradient TC4/TC4-5Cu coupons fabricated by selective laser melting(SLM)through various electrochemical measurements,surface examination,observation of biofilm and corrosion analysis.The results indicated that the Cu-bearing alloy showed an inhibitory effect on the biofilms due to the release of Cu element,thereby reducing the corrosion rate of MIC.The corrosion current density(icorr)of TC4(11.7±0.8)nA cm−2 is higher than that of TC4/TC4-5Cu(7.4±0.4)nA cm−2 in the presence of S.mutans,while the maximum pit depth of TC4 is 1.6 times that of TC4/TC4-5Cu.Therefore,metal modification through Cu alloying is an effective strategy to improve the MIC resistance. | Xing Zhou Qiyue Zhang Jiarui Lu Ying Zheng Lin Wu Dake Xu Xue Zhang Qiang Wang | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，12： | 0 |
| 12 | Lightweight Intrusion Detection Using Reservoir Computing显示文摘The blockchain-empowered Internet of Vehicles(IoV)enables various services and achieves data security and privacy,significantly advancing modern vehicle systems.However,the increased frequency of data transmission and complex network connections among nodes also make them more susceptible to adversarial attacks.As a result,an efficient intrusion detection system(IDS)becomes crucial for securing the IoV environment.Existing IDSs based on convolutional neural networks(CNN)often suffer from high training time and storage requirements.In this paper,we propose a lightweight IDS solution to protect IoV against both intra-vehicle and external threats.Our approach achieves superior performance,as demonstrated by key metrics such as accuracy and precision.Specifically,our method achieves accuracy rates ranging from 99.08% to 100% on the Car-Hacking dataset,with a remarkably short training time. | Jiarui Deng Wuqiang Shen Yihua Feng Guosheng Lu Guiquan Shen Lei Cui Shanxiang Lyu | 2024 | Computers, Materials & Continua2024，78，1： | 0 |
| 13 | DeepFake Videos Detection Based on Texture Features显示文摘In recent years,with the rapid development of deep learning technologies,some neural network models have been applied to generate fake media.DeepFakes,a deep learning based forgery technology,can tamper with the face easily and generate fake videos that are difficult to be distinguished by human eyes.The spread of face manipulation videos is very easy to bring fake information.Therefore,it is important to develop effective detection methods to verify the authenticity of the videos.Due to that it is still challenging for current forgery technologies to generate all facial details and the blending operations are used in the forgery process,the texture details of the fake face are insufficient.Therefore,in this paper,a new method is proposed to detect DeepFake videos.Firstly,the texture features are constructed,which are based on the gradient domain,standard deviation,gray level co-occurrence matrix and wavelet transform of the face region.Then,the features are processed by the feature selection method to form a discriminant feature vector,which is finally employed to SVM for classification at the frame level.The experimental results on the mainstream DeepFake datasets demonstrate that the proposed method can achieve ideal performance,proving the effectiveness of the proposed method for DeepFake videos detection. | Bozhi Xu Jiarui Liu Jifan Liang Wei Lu Yue Zhang | 2021 | Computers, Materials & Continua2021，，7： | 0 |
| 14 | Design, Synthesis, Inhibiting HDACs Ability and Antitumor Activity of Pyrimidin-4(3H)-one Hydroxamate Derivatives显示文摘 | LU Haibin JIN Xiangqun JIN Yang HUANG Bangrui WANG Cheng WANG Chunhe MA Fangyuan CHEN Yuting LI Jiarui CONG Yu WANG Wenlong SONG Yuming MU Xupeng | 2016 | Chemical Research in Chinese Universities2016，32，4： | 0 |
| 15 | Identification of Bulbocodin D and C as novel STAT3 inhibitors and their anticancer activities in lung cancer cells显示文摘Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer agents. Bulbocodin D (BD) and Bulbocodin C (BC), two bibenzyls derived from Pleione bulbocodioides (Franch.) Rolfe, have demonstrated notable in vitro anticancer activity. In human lung cancer A549 cells, the IC50s for BD and BC were 11.63 and 11.71 μmol·L^(−1), respectively. BD triggered apoptosis, as evidenced by an upsurge in Annexin V-positive cells and elevated protein expression of cleaved-PARP in cancer cells. Furthermore, BD and BC markedly inhibited the migratory and invasive potentials of A549 cells. The altered genes identified through RNA-sequencing analysis were integrated into the CMap dataset, suggesting BD’s role as a potential signal transducer and activator of transcription 3 (STAT3) inhibitor. SwissDock and MOE analyses further revealed that both BD and BC exhibited a commendable binding affinity with STAT3. Additionally, a surface plasmon resonance assay confirmed the direct binding affinity between these compounds and STAT3. Notably, treatment with either BD or BC led to a significant reduction in p-STAT3 (Tyr 705) protein levels, regardless of interleukin-6 stimulation in A549 cells. In addition, the extracellular signal-regulated kinase (ERK) was activated after BD or BC treatment. An enhancement in cancer cell mortality was observed upon combined treatment of BD and U0126, the MEK1/2 inhibitor. In conclusion, BD and BC emerge as promising novel STAT3 inhibitors with potential implications in cancer therapy. | HE Xinyu FU Jiarui LYU Wenyu HUANG Muyang MO Jianshan CHENG Yaxin XU Yulian ZHENG Lijun ZHANG Xiaolei QI Lu ZHANG Lele ZHENG Ying HUANG Mingqing NI Lin LU Jinjian | 2023 | Chinese Journal of Natural Medicines2023，21，11： | 0 |
| 16 | Switchable nanoparticles complexing cisplatin for circumventing glutathione depletion in breast cancer chemotherapy显示文摘Cisplatin is broad-spectrum chemotherapeutic agent that has been widely used for the treatment of a variety of malignant tumors including breast cancer.However,the cisplatin chemoresistance,which derives from the inactivation by glutathione(GSH)depletion,remains a scientific issue to solve.Here,we report a novel type of smart disulfide switchable nanoparticles complexing cisplatin(switch NPs-cisplatin)that is rationally designed,and engineered by synthesizing a hyaluronic acid disulfide bonded polyaspartic acid(HA-ss-Pasp)and complexing cisplatin.The results showed that the switch NPs-cisplatin had a nanoscale of particle size(150 nm),higher drug encapsulation efficiency(>90%),and suitable drug release profile.They demonstrated evident pH responsiveness and GSH responsiveness,and targeting effect in the resistant breast cancer cells.Furthermore,they were able to block the cisplatin depletion by GSH in the resistant cancer cells,thereby circumventing the chemoresistance.Consequently,switch NPs-cisplatin displayed a remarkable killing effect in the resistant breast cancer cells in vitro,and in the resistant breast cancer-bearing mice.In conclusion,switch NPs-cisplatin could be used as a smart formulation of cisplatin for overcoming the chemoresistance of breast cancer.The present study also offers a universal drug delivery carrier platform for highly efficient but low systemic toxic chemotherapy. | Ming Chen Ying Xie Qian Luo Jiarui Xu Yuxin Ren Rui Liu Huihui Zhao Yuling Chen Hexuan Feng Yafei Du Jianwei Li Guiling Wang Wanliang Lu | 2023 | Chinese Chemical Letters2023，34，5： | 0 |
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