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| 1 | Ectopic expression of VRT-A2 underlies the origin of Triticum polonicum and Triticum petropavlovskyi with long outer glumes and grains显示文摘Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants. | Jing Liu Zhaoyan Chen Zhihui Wang Zhaoheng Zhang Xiaoming Xie Zihao Wang Lingling Chai Long Song Xuejiao Cheng Man Feng Xiaobo Wang Yanhong Liu Zhaorong Hu Jiewen Xing Zhenqi Su Huiru Peng Mingming Xin Yingyin Yao Weilong Guo Qixin Sun Jie Liu Zhongfu Ni | 2021 | Molecular Plant2021，14，9： | 4 |
| 2 | Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus显示文摘 | Liping Yan Shan Peng Pixi Yan Jiewen Zhou Qiaoyang Teng Guoxin Li Xuesong Li Zejun Li | 2012 | Journal of Virological Methods (-)2012，，1： | 1 |
| 3 | Classical CMF regimen as adjuvant chemotherapy for triple-negative breast cancer may be more effective compared with anthracycline or taxane-based regimens显示文摘 | Shusen Wang Yanxia Shi Zhongyu Yuan Xi Wang Donggen Liu Roujun Peng Xiaoyu Teng Tao Qin Jiewen Peng Guinan Lin Xiaomei Jiang | 2012 | Medical Oncology2012，，2： | 1 |
| 4 | Protein macrocyclization by a recombinant asparaginyl endopeptidase显示文摘Protease is abundant in nature and has a wide variety,playing a vital role in a series of cell processes.Most proteases cause protein degradation by cleaving substrate sequence within the target or altering proteins into a biologically active mature form.In addition,some proteases also have special functions.For instance,the ligase-type asparagine endopeptidases(AEPs)have been recently implicated in catalyzing precursor peptides to form cyclotides,such as the well-studied prototypical cyclotide,kalata B1(kB1)from Oldenlandia affinis encoded by the Oak1 gene[1].Backbone cyclization renders the cyclotides extreme stability,thus enabling them to be applied as ideal drug design frameworks in pharmaceutical engineering[2].Therein,it would be of great benefits if such AEPs could be developed as biotechnological tools to catalyze the cyclization of peptides or proteins.Efforts have been made to attain active AEPs,mainly depending on direct extraction of the enzymes from plant tissues.The process is tedious,but the AEP yields are generally low.Although attempts aiming at recombinantly producing AEPs have been made,the produced AEPs are either functionally inactive[3]or of very limited efficacy[4],which largely limits the applications of the AEPs.Fortunately,a recent study reported that an AEP(OaAEP1b)was recombinantly produced as a His6-Ubiquitin-OaAEP1b fusion protein in Escherichia coli and exhibited good cyclizing ability[1],thereby paving the way to obtain recombinant AEPs through microbial expression. | Xiaoyun Hu Hui Liu Jie Li Jiewen Wang Wenfang Peng | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 5 | Chaperone-mediated Autophagy Regulates Cell Growth by Targeting SMAD3 in Glioma显示文摘Previous studies suggest that the reduction of SMAD3(mothers against decapentaplegic homolog 3)has a great impact on tumor development,but its exact pathological function remains unclear.In this study,we found that the protein level of SMAD3 was greatly reduced in human-grade IV glioblastoma tissues,in which LAMP2A(lysosome-associated membrane protein type 2A)was significantly up-regulated.LAMP2A is a key ratelimiting protein of chaperone-mediated autophagy(CMA),a lysosome pathway of protein degradation that is activated in glioma.We carefully analyzed the amino-acid sequence of SMAD3 and found that it contained a pentapeptide motif biochemically related to KFERQ,which has been proposed to be a targeting sequence for CMA.In vitro,we confirmed that SMAD3 was degraded in either serum-free or KFERQ motif deleted condition,which was regulated by LAMP2A and interacted with HSC70(heat shock cognate 71 kDa protein).Using isolated lysosomes,amino-acid residues 75 and 128 of SMAD3 were found to be of importance for this process,which affected the CMA pathway in which SMAD3 was involved.Similarly,down-regulating SMAD3 or up-regulating LAMP2A in cultured glioma cells enhanced their proliferation and invasion.Taken together,these results suggest that excessive activation of CMA regulates glioma cell growth by promoting the degradation of SMAD3.Therefore,targeting the SMAD3-LAMP2Amediated CMA-lysosome pathway may be a promising approach in anti-cancer therapy. | Hanqun Liu Yuxuan Yong Xingjian Li Panghai Ye Kai Tao Guoyou Peng Mingshu Mo Wenyuan Guo Xiang Chen Yangfu Luo Yuwan Lin Jiewen Qiu Ziling Zhang Liuyan Ding Miaomiao Zhou Xinling Yang Lin Lu Qian Yang Pingyi Xu | 2022 | Neuroscience Bulletin2022，38，6： | 0 |
| 6 | TaANR1-TaMADS25 module regulates lignin biosynthesis and root development in wheat(Triticum aestivum L.)显示文摘Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is responsible for water and nutrient acquisition,thus crucial for competitive fitness and crop yield in challenging environments(Karlova et al.,2021;Liu et al.,2022).Over the past decades,extensive research has focused on identifying genes accountable for root growth and development in plants(Rogers and Benfey,2015).However,only a limited number of genes have been cloned in wheat(Li et al.,2021). | Weiya Xu Yongming Chen Bin Liu Qiuyuan Li Yilan Zhou Xuanshuang Li Weilong Guo Zhaorong Hu Zhenshan Liu Mingming Xin Yingyin Yao Mingshan You Huiru Peng Zhongfu Ni Jiewen Xing | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
| 7 | The TaTCP4/10-B1 cascade regulates awn elongation in wheat(Triticum aestivum L.)显示文摘Awns are important morphological markers for wheat and exert a strong physiological effect on wheat yield.The awn elongation suppressor B1 has recently been cloned through association and linkage analysis in wheat.However,the mechanism of awn inhibition centered around B1 remains to be clarified.Here,we identified an allelic variant in the coding region of B1 through analysis of re-sequencing data;this variant causes an amino acid substitution and premature termination,resulting in a long-awn phenotype.Transcriptome analysis indicated that B1 inhibited awn elongation by impeding cytokinin-and auxinpromoted cell division.Moreover,B1 directly repressed the expression of TaRAE2 and TaLks2,whose orthologs have been reported to promote awn development in rice or barley.More importantly,we found that TaTCP4 and TaTCP10 synergistically inhibited the expression of B1,and a G-to-A mutation in the B1 promoter attenuated its inhibition by TaTCP4/10.Taken together,our results reveal novel mechanisms of awn development and provide genetic resources for trait improvement in wheat. | Wensheng Ke Jiewen Xing Zhaoyan Chen Yidi Zhao Weiya Xu Lulu Tian Jinquan Guo Xiaoming Xie Dejie Du Zihao Wang Yufeng Li Jin Xu Mingming Xin Weilong Guo Zhaorong Hu Zhenqi Su Jie Liu Huiru Peng Yingyin Yao Qixin Sun Zhongfu Ni | 2023 | Plant Communications2023，4，4： | 0 |
| 8 | Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes显示文摘Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were established.Gr and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and encapsulation.PI cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign objects.These results also suggested that granulocytes and plasmatocytes may express some different proteins.By comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis.These genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on. | Jian Hu Yan Du Meng Meng Yipei Dong Jiewen Peng | 2021 | Insect Science2021，28，5： | 0 |
| 9 | CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson's disease显示文摘Mitochondrial dysfunction is a significant pathological alte ration that occurs in Parkinson's disease(PD),and the Thr61lle(T61I)mutation in coiled-coil helix coiled-coil helix domain containing 2(CHCHD2),a crucial mitochondrial protein,has been reported to cause Parkinson's disease.FIFO-ATPase participates in the synthesis of cellular adenosine triphosphate(ATP)and plays a central role in mitochondrial energy metabolism.However,the specific roles of wild-type(WT)CHCHD2 and T611-mutant CHCHD2 in regulating F1FO-ATPase activity in Parkinson's disease,as well as whether CHCHD2 or CHCHD2 T61I affects mitochondrial function through regulating F1FO-ATPase activity,remain unclea r.Therefore,in this study,we expressed WT CHCHD2 and T61l-mutant CHCHD2 in an MPP^(+)-induced SH-SY5Y cell model of PD.We found that CHCHD2 protected mitochondria from developing MPP^(+)-induced dysfunction.Under normal conditions,ove rexpression of WT CHCHD2 promoted F1FO-ATPase assembly,while T61I-mutant CHCHD2 appeared to have lost the ability to regulate F1FO-ATPase assembly.In addition,mass spectrometry and immunoprecipitation showed that there was an interaction between CHCHD2 and F1FO-ATPase.Three weeks after transfection with AAV-CHCHD2 T61I,we intraperitoneally injected 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into mice to establish an animal model of chronic Parkinson's disease and found that exogenous expression of the mutant protein worsened the behavioral deficits and dopaminergic neurodegeneration seen in this model.These findings suggest that WT CHCHD2 can alleviate mitochondrial dysfunction in PD by maintaining F1F0-ATPase structure and function. | Xiang Chen Yuwan Lin Zhiling Zhang Yuting Tang Panghai Ye Wei Dai Wenlong Zhang Hanqun Liu Guoyou Peng Shuxuan Huang Jiewen Qiu Wenyuan Guo Xiaoqin Zhu Zhuohua Wu Yaoyun Kuang Pingyi Xu Miaomiao Zhou | 2024 | Neural Regeneration Research2024，19，1： | 0 |
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