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| 1 | Reversal of multidrug resistance in drug-resistant human gastric cancer cell line SGC7901/VCR by antiprogestin drug mifepristone显示文摘AIM: To explore the reversal effect of mifepristone on multidrug resistance (MDR) in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanisms. METHODS: Expression of multidrug resistance-associated protein(MRP) was detected using reverse transcriptionpolymerase chain reaction(RT-PCR). Flow cytometry was used to assay the expression of P-glycoprotein(P-gp), Bcl-2,Bax, and the mean fluorescent intensity of intracellular rhodamine 123 in the cells. Meanwhile, the protein levels of Bcl-2 and Bax were also detected by Western blotting analysis. The sensitivity of cells to the anticancer agent, vincrimycin(VCR), and the intracellular [^3H]VCR accumulation were determined by tetrazolium blue (MTT) assay and a liquid scintillation counter, respectively. RESULTS: Expression of MRP and P-gp in SGC7901/VCR cells was 6.04-and 8.37-fold higher as compared with its parental SGC7901 cells, respectively. After treatment with 1, 5, 10, and 20 umol/L mifepristone, SGC7901/VCR cells showed a 1.34-, 2.29-, 3.11-, and 3.71-fold increase in the accumulation of intracellular VCR, a known substrate of MRP, and a 1.03-, 2.04-, 3.08-, and 3.68-fold increase in the retention of rhodamine 123, an indicator of P-gp function, respectively. MTT assay revealed that the resistance of SGC7901/VCR cells to VCR was 11.96-fold higher than that of its parental cells. The chemosensitivity of SGC7901/VCR cells to VCR was enhanced by 1.02-, 7.19-, 12.84-, and 21.17-fold after treatment with mifepristone at above-mentioned dose. After 96 h of incubation with mifepristone 10 umol/L, a concentration close to plasma concentrations achievable in human, the expression of Bcl-2 protein was decreased to (9.21+0.65)% from (25.32+1.44)%, whereas the expression of Bax protein was increased to (19.69+1.13)% from (1.24+0.78)% (P<0.01). Additionally, the effects of mifepristone on the expression of Bcl-2 and Bax proteins in SGC7901/VCR cells were further demonstrated by Western blotting analysis. CONCLUSION: Mifepristone has potent reversal effect on MDR in SGC7901/VCR via inhibiting the function of MRP and P-gp, modulating the expression of Bcl-2 and Bax proteins, and enhancing the sensitivity to anticancer agent VCR. | Da-QiangLi Zhi-BiaoWang JinBai JieZhao YuanWang KaiHu Yong-HongDu | 2004 | World Journal of Gastroenterology2004，10，12： | 29 |
| 2 | Effects of mifepristone on invasive and metastatic potential of human gastric adenocarcinoma cell line MKN-45 in vitro and in vivo显示文摘AIM: To investigate the effects of mifepristone on the invasive and metastatic potential of human gastric adenocarcinoma cell line MKN-45 and its mechanisms. METHODS: After incubation with various concentrations of mifepristone (5, 10, 20 umol/L), the adhesion to artificial basement membrane, Matrigel, and the migration of MKN-45 cells were assayed using MTT assay and Transwell cell culture chambers, respectively. Enzyme- linked immunoabsorbent assay (ELISA) and flow cytometry were used to determine the expression of vascular endothelial growth factor (VEGF) and integrin 133 in the cells. After subcutaneous transplantation of MKN-45 cells in nude mice, mifepristone (50 mg/kg.d) was administrated subcutaneously for 8 wk to assess its effects on tumor metastasis. Immunohistochemical analysis was used to detect the expression of VEGF and microvascular density (MVD) in xenografted tumors. RESULTS: Mifepristone dose-dependently inhibited the heterotypic adhesion to Matrigel of MKN-45 cells. The inhibition was accompanied by a significant down-regulation of integrin 133 expression in the cells. After incubation with 5, 10, 20 umol/L mifepristone, the number of migrated MKN-45 cells was 72+8, 50+6, 41+5 in experiment group, and 94+16 in control group (P<0.01). Meanwhile, secreted VEGF protein of MKN-45 cells in mifepristone-treated group (14.2+2.9, 8.9+3.1, 5.4+2.1 ng/g per liter) was significantly lower than that in control group (22.7+4.3 ng/g per liter, P<0.01). In vivo, mifepristone decreased the number of metastatic foci in lungs of nude mice and down-regulated the expression of VEGF and MVD in the xenograted tumors. CONCLUSION: Mifepristone can effectively inhibit the invasive and metastatic potential of human gastric adenocarcinoma cell line MKN-45 in vitro and in vivo through inhibition of heterotypic adhesion to basement membrane, cell migration and angiogenesis. | Da-QiangLi Zhi-BiaoWang JinBai JieZhao YuanWang KaiHu Yong-HongDu | 2004 | World Journal of Gastroenterology2004，10，12： | 10 |
| 3 | Effects of mifepristone on proliferation of human gastric adenocarcinoma cell line SGC-7901 in vitro显示文摘AIM: To explore the effects of mifepristone, a progesterone receptor (PR) antagonist, on the proliferation of human gastric adenocarcinoma cell line SGC-7 901 in vitro and the possible mechanisms involved.METHODS: In situ hybridization was used to detect the expression of PR mRNA in SGC-7 901 cells. After treatment with various concentrations of mifepristone (2.5, 5, 10,20μmol/L) at various time intervals, the ultrastructural changes, cell proliferation, cell-cycle phase distribution, and the expression of caspase-3 and Bcl-XL were analyzed using transmission electron microscopy (TEM), tetrazolium blue (MTT) assay, ^3H-TdR incorporation, flow cytometry, and reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: Mifepristone markedly induced apoptosis and inhibited cell proliferation of PR- positive SGC-7 901 cells revealed by TEM, MTT assay and ^3H-TdR incorporation, in a dose- and time-dependent manner. The inhibitory rate was increased from 8.98% to 51.29%. Flow cytometric analysis showed mifepristone dose-dependently decreased cells in S and G2/M phases, increased cells in G0/G1 phase,reduced the proliferative index from 57.75% to 22.83%.In addition, mifepristone up-regulated the expression of caspase-3, and down- regulated the Bcl-XL expression,dose-dependently.CONCLUSION: Mifepristone effectively inhibited the proliferation of PR-positive human gastric adenocarcinoma cell line SGC-7 901 in vilrothrough multiple mechanisms, and may be a beneficial agent against human adenocarcinoma. | Da-QiangLi Zhi-BiaoWang JinBai JieZhao YuanWang KaiHu Yong-HongDu | 2004 | World Journal of Gastroenterology2004，10，18： | 5 |
| 4 | Use of High Intensity Focused Ultrasound for Treating Malignant Tumors显示文摘OBJECTIVE To investigate the efficacy and side effects of high intensity focused ultrasound(HIFU) in the treatment of malignant solid tumors. METHODS Thirty patients who refused surgery and/or were refractory to chemotherapy were treated by HIFU alone, with the efficacy and side effects monitored as follows: observation of vital organ signs; functional assay of important organs; imaging examinations including: digital subtraction angiography (DSA), CT, MRI, single photon emission computed tomography (SPECT), large core needle biopsy, complications and metastasis. RESULTS After HIFU therapy, the vital signs remained stable and the functions of the heart, lung, kidney and liver were also normal. DSA images showed that small or larger arteries were not damaged. After a follow-up of 10-38 months(mean 23.1 months), 26 patients(87%) were alive. The volume of the tumor underwent complete regression in 10 patients. Shrinkage of the tumor volume ≥50% was observed in 13 patients. Eight of 13 patients were examined by large core needle biopsy, all showing necrosis and/or fibrosis though 3 patients(10%) had local recurrence. Two of these were retreated again by HIFU and the locally recurrent tumors were controlled. New metastases developed in 5 patients after H IFU. Two patients suffered from peripheral nerve injuriy and they have recovered during the follow-up. One patient developed skin injury. CONCLUSION High intensity focused ultrasound is effective and safe in the treatment of malignant solid tumors. | WenzhiChen ZhibiaoWang FengWu JinBai HuiZhu JianzhongZou KequanLi FanglinXie ZhilongWang | 2004 | Chinese Journal of Clinical Oncology2004，1，1： | 3 |
| 5 | Cadmium Biosorpfion Rate in Protonated Sargassum Biomass显示文摘 | Jinbai Yang Bohumil Volesky | 1998 | Environmental Scienceand Technology1998，，： | 1 |
| 6 | Biosorption of Uranium on Sargeaaum biomass显示文摘 | Jinbai Yang Bohumil Volesky | 1999 | Water Research1999，33，15： | 1 |
| 7 | Biosorption of uranium on sargassum biomass显示文摘 | Jinbai Yang Bohumil Volesky | 1999 | Water Research1999，33，15： | 1 |
| 8 | Biosorption of uranium on sargassum biomass 显示文摘 | Yang Jinbai Volesky Bohumil | 1999 | Water Research1999，33，15： | 1 |
| 9 | Binary tree of SVM: A new fast multiclass training and classification algorithm显示文摘 | Ben Fei Liu Jinbai | 2006 | IEEE Trans on Neu- ral Networks2006，17，3： | 1 |
| 10 | Annual water budget of a small basin in the northern loess plateau in China 显示文摘 | Osamu Hinokidani Jinbai Huang Hiroshi Yasuda | 2010 | Journal of Arid Land Studies2010，20，3： | 1 |
| 11 | Effects of the Check Dam System on Water Redistribution in the Chinese Loess Plateau显示文摘 | Jinbai Huang Osamu Hinokidani Hiroshi Yasuda Chandra S. P. Ojha Yuki Kajikawa Shiqing Li | 2013 | Journal of Hydrologic Engineering2013，，8： | 1 |
| 12 | Binary Tree of SVM: A New Fast Multiclass Training and Classification Algorithm显示文摘 | Fei Ben Liu Jinbai | 2006 | IEEE Transactions on Neural Networks2006，17，3： | 1 |
| 13 | Biosorption of uranium on sargassum biomass显示文摘 | YANG Jinbai VOLESKY Bohulil | 1999 | Wat Res1999，33，2： | 1 |
| 14 | Bi osorption of uranium on Sargassum biomass显示文摘 | Jinbai Yang Bohumil Volesky | 1999 | Water Research1999，33，15： | 1 |
| 15 | Effects of the check dam system on water redistribution in the Chinese Loess Plateau显示文摘 | Huang Jinbai Hinokidani O Yasuda H | 2013 | Journal of Hydrologic Engi- neering2013，18，8： | 1 |
| 16 | Elliptic model for space-time correlations in turbulent shear flows显示文摘 | He Guowei Zhang Jinbai | 2006 | Physical Review E2006，73，05： | 1 |
| 17 | Run- off and water budget of the Liudaogou Catchment at the wind-water erosion crisscross region on the Loess Plat- eau of China显示文摘 | Huang Jinbai Wen Jiawei Hinokidani O | 2014 | Environmental Earth Sciences2014，72，9： | 1 |
| 18 | Extracorporeal High-Intensity Focused Ultrasound Treatment for Breast Cancer显示文摘OBJECTIVE To evaluate the clinical safety and efficacy of using highintensity focused ultrasound (HIFU) therapy, for breast cancer, and to select the appropriate methods in evaluating the therapeutic effects.METHODS A total of 24 patients with breast cancer underwent HIFU treatment 1-2 weeks before receiving modified radical mastectomy. During and after HIFU therapy, changes in blood pressure, breath, pulse and peripheral blood oxygen saturation were monitored. At the same time, the damage of the skin and tissue produced by HIFU at the target region was evaluated as well. Surgically excised samples were used for pathological examinations to evaluate the HIFU-induced destruction of the targeted tissue. Three patients received Tc-ECT and 1 MRI examinations before and after HIFU.RESULTS HIFU treatment had no apparent influence on either the tissue nearby the target or on vital signs of the patients. Pathological, tc-ECT and MRI examinations demonstrated that targeted tissue showed complete coagulative necrosis.CONCLUSION Under the guidance of real-time ultrasonic imaging, HIFU can effectively and safely destroy the breast cancer mass and ^99MTc-ECT and MRI examination can be utilized to evaluate the therapeutic effects.HIFU may become one of the options for breast cancer therapy in the future. | HuiZhu FengWu WenzhiChen YoudeCao JinBai ZhibiaoWang | 2004 | Chinese Journal of Clinical Oncology2004，1，5： | 1 |
| 19 | Binary tree of SVM:A new fast multiclass training and classification algorithm 显示文摘 | Fei Ben Liu Jinbai | 2006 | IEEE Transaction on Neural Networks2006，，17： | 1 |
| 20 | Biosorption of uranium on Sargassum biomass显示文摘 | Jinbai Yang Bohumil Volesky | 1999 | Water Research1999，，15： | 1 |
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