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| 1 | Improvement of wheat drought and salt tolerance by expression of a stress- inducible transcription factorGmDREB of soybean (Glycinemax)显示文摘Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants. | GAO Shiqing XU Huijun CHENG Xianguo CHEN Ming XU Zhaoshi LI Liancheng YE Xingguo DU Lipu HAO Xiaoyan MA Youzhi | 2005 | Chinese Science Bulletin2005，50，23： | 30 |
| 2 | Eco-environment range in the source regions of the Yangtze and Yellow rivers显示文摘Based on geographical and hydrological extents delimited, four principles are identified, as the bases for delineating the ranges of the source regions of the Yangtze and Yellow rivers in the paper. According to the comprehensive analysis of topographical characteristics, climate conditions, vegetation distribution and hydrological features, the source region ranges for eco-environmental study are defined. The eastern boundary point is Dari hydrological station in the upper reach of the Yellow River. The watershed above Dari hydrological station is the source region of the Yellow River which drains an area of 4.49×10 4 km 2 . Natural environment is characterized by the major topographical types of plateau lakes and marshland, gentle landforms, alpine cold semi-arid climate, and steppe and meadow vegetation in the source region of the Yellow River. The eastern boundary point is the convergent site of the Nieqiaqu and the Tongtian River in the upstream of the Yangtze River. The watershed above the convergent site is the source region of the Yangtze River, with a watershed area of 12.24×10 4 km 2 . Hills and alpine plain topography, gentle terrain, alpine cold arid and semi-arid climate, and alpine cold grassland and meadow are natural conditions in the source region of the Yangtze River. | DING Yongjian,YANG Jianping,LIU Shiyin,CHEN Rensheng,WANG Genxu,SHEN Yongping,WANG Jian,XIE Changwei,ZHANG Shiqing(Cold and Arid Regions Environmental and Engineering Research Institute, CAS, Lanzhou 730000, China) | 2003 | Journal of Geographical Sciences2003，13，2： | 18 |
| 3 | Progression in translational research on spinal cord injury based on microenvironment imbalance显示文摘Spinal cord injury(SCI) leads to loss of motor and sensory function below the injury level and imposes a considerable burden on patients, families, and society. Repair of the injured spinal cord has been recognized as a global medical challenge for many years.Significant progress has been made in research on the pathological mechanism of spinal cord injury. In particular, with the development of gene regulation, cell sequencing, and cell tracing technologies, in-depth explorations of the SCI microenvironment have become more feasible. However, translational studies related to repair of the injured spinal cord have not yielded significant results. This review summarizes the latest research progress on two aspects of SCI pathology: intraneuronal microenvironment imbalance and regenerative microenvironment imbalance. We also review repair strategies for the injured spinal cord based on microenvironment imbalance, including medications, cell transplantation, exosomes, tissue engineering, cell reprogramming, and rehabilitation. The current state of translational research on SCI and future directions are also discussed. The development of a combined, precise, and multitemporal strategy for repairing the injured spinal cord is a potential future direction. | Baoyou Fan Zhijian Wei Shiqing Feng | 2022 | Bone Research2022，10，2： | 17 |
| 4 | Enhanced mechanical properties and degradation rate of Mg-Ni-Y alloy by introducing LPSO phase for degradable fracturing ball applications显示文摘In this work,as-cast Mg-Ni-Y alloys were proposed to develop a feasible material for fracturing balls,and their mechanical performance and corrosion behavior were systematically investigated.Long period stacking order(LPSO)phase was firstly introduced to improve both the mechanical properties and degradation rate of magnesium alloys.With the increase of LPSO phase,the compressive strength was improved significantly,while the elongation of the alloys decreased owing to the relatively brittle nature of LPSO phase.Due to the higher corrosion potential of LPSO phase,the LPSO phase can accelerate the corrosion process by providing more micro-couples.However,the LPSO phase would serve as the corrosion barrier between the corrosion medium and the matrix when the contents of LPSO phase are too high in Mg92.5Ni3Y4.5 and Mg87.5Ni5Y7.5 alloys.As-cast Mg97.5Ni1Y1.5 alloy with satisfactory mechanical properties and rapid degradation rate was successfully developed,exhibiting a high degradation rate of 6675 mm/a(93℃)in 3 wt.%KCl solution and a favorable ultimate compressive strength of 410 MPa.The degradation rate of Mg97.5Ni1Y1.5 alloy is 2-5 times of the current commercial magnesium alloy fracturing materials. | Jingfeng Wang Shiqing Gao Xiuying Liu Xing Peng Kui Wang Shijie Liu Weiyan Jiang Shengfeng Guo Fusheng Pan | 2020 | Journal of Magnesium and Alloys2020，8，1： | 13 |
| 5 | Assessing environmental factors associated with regional schistosomiasis prevalence in Anhui Province,Peoples’Republic of China using a geographical detector method显示文摘Background:Schistosomiasis is a water-borne disease caused by trematode worms belonging to genus Schistosoma,which is prevalent most of the developing world.Transmission of the disease is usually associated with multiple biological characteristics and social factors but also factors can play a role.Few studies have assessed the exact and interactive influence of each factor promoting schistosomiasis transmission.Methods:We used a series of different detectors(i.e.,specific detector,risk detector,ecological detector and interaction detector)to evaluate separate and interactive effects of the environmental factors on schistosomiasis prevalence.Specifically,(i)specific detector quantifies the impact of a risk factor on an observed spatial disease pattern,which were ranked statistically by a value of Power of Determinate(PD)calculation;(ii)risk detector detects high risk areas of a disease on the condition that the study area is stratified by a potential risk factor;(iii)ecological detector explores whether a risk factor is more significant than another in controlling the spatial pattern of a disease;(iv)interaction detector probes whether two risk factors when taken together weaken or enhance one another,or whether they are independent in developing a disease.Infection data of schistosomiasis based on conventional surveys were obtained at the county level from the health authorities in Anhui Province,China and used in combination with information from Chinese weather stations and internationally available environmental data.Results:The specific detector identified various factors of potential importance as follows:Proximity to Yangtze River(0.322)>Land cover(0.285)>sunshine hours(0.256)>population density(0.109)>altitude(0.090)>the normalized different vegetation index(NDVI)(0.077)>land surface temperature at daytime(LST_(day))(0.007).The risk detector indicated that areas of schistosomiasis high risk were located within a buffer distance of 50 km from Yangtze River.The ecological detector disclosed that the factors investigated have significantly different effects.The interaction detector revealed that interaction between the factors enhanced their main effects in most cases.Conclusion:Proximity to Yangtze River had the strongest effect on schistosomiasis prevalence followed by land cover and sunshine hours,while the remaining factors had only weak influence.Interaction between factors played an even more important role in influencing schistosomiasis prevalence than each factor on its own.High risk regions influenced by strong interactions need to be targeted for disease control intervention. | Yi Hu Congcong Xia Shizhu Li Michael PWard Can Luo Fenghua Gao Qizhi Wang Shiqing Zhang Zhijie Zhang | 2017 | Infectious Diseases of Poverty2017，6，1： | 9 |
| 6 | Estimation of water balance in the source region of the Yellow River based on GRACE satellite data显示文摘Water storage has important significance for understanding water cycles of global and local domains and for monitoring climate and environmental changes.As a key variable in hydrology,water storage change represents the sum of precipitation,evaporation,surface runoff,soil water and groundwater exchanges.Water storage change data during the period of 2003-2008 for the source region of the Yellow River were collected from Gravity Recovery and Climate Experiment(GRACE)satellite data.The monthly actual evaporation was estimated according to the water balance equation.The simulated actual evaporation was significantly consistent and correlative with not only the observed pan(20 cm)data,but also the simulated results of the version 2 of Simple Biosphere model.The average annual evaporation of the Tangnaihai Basin was 506.4 mm,where evaporation in spring,summer,autumn and winter was 130.9 mm,275.2 mm,74.3 mm and 26.1 mm,and accounted for 25.8%,54.3%,14.7% and 5.2% of the average annual evaporation,respectively.The precipitation increased slightly and the actual evaporation showed an obvious decrease.The water storage change of the source region of the Yellow River displayed an increase of 0.51 mm per month from 2003 to 2008,which indicated that the storage capacity has significantly increased,probably caused by the degradation of permafrost and the increase of the thickness of active layers.The decline of actual evaporation and the increase of water storage capacity resulted in the increase of river runoff. | Min XU BaiSheng YE QiuDong ZHAO ShiQing ZHANG Jiang WANG | 2013 | Journal of Arid Land2013，5，3： | 8 |
| 7 | Novel roll effector based on zero-mass-flux dual synthetic jets and its flight test显示文摘The autonomous and controllable Dual Synthetic Jet Actuator(DSJA)is firstly integrated into the Unmanned Aerial Vehicle(UAV),and flight tests without the deflection of rudders are carried out to verify the viability of DSJA to control the attitudes of UAV during cruising.DSJA is improved into an actuator with two diaphragms and three cavities,which has higher energy levels.Actuators,differentially distributed on both sides of the wings,are installed on the trailing edge close to the wing tips.Flight tests,containing Differential Circulation Control(DCC)using double-side actuators,Positive Circulation Control(PCC)using left-side actuators and Negative Circulation Control(NCC)using right-side actuators,are implemented at cruising speed of 25 m/s.Results show that roll attitude control without rudders could be realized by DSJAs.DCC and NCC can generate the rightward roll and yaw angular velocity,prompting UAV to turn right.The stronger controlling ability can be achieved by DCC,with the maximum roll angular velocity of 15.62(°)/s.PCC can generate a rightward roll moment,but a leftward yaw moment will be produced at the same time.Leftward yaw induces the leftward rolling moment,which weakens the roll control effect,making UAV keep to yaw to the left with a small slope. | Zhenbing LUO Zhijie ZHAO Jiefu LIU Xiong DENG Mu ZHENG Hang YANG Qingyang CHEN Shiqing LI | 2022 | Chinese Journal of Aeronautics2022，35，8： | 7 |
| 8 | Electrochemical reduction of CO_2 in solid oxide electrolysis cells显示文摘The effort on electrochemical reduction of CO_2 to useful chemicals using the renewable energy to drive the process is growing fast recently. In this review, we introduce the recent progresses on the electrochemical reduction of CO_2 in solid oxide electrolysis cells(SOECs). At high temperature, only CO is produced with high current densities and Faradic efficiency while the reactor is complicated and a better sealing technique is urgently needed. The typical electrolytes such as zirconia-based oxides, ceria-based oxides and lanthanum gallates-based oxides, anodes and cathodes are introduced in this review, and the cathode materials, such as conventional metal–ceramics(cermets), mixed ionic and electronic conductors(MIECs) are discussed in detail. In the future, to gain more value-added products, the electrolyte, cathode and anode materials should be developed to allow SOECs to be operated at temperature range of 573–873 K. At those temperatures, SOECs may combine the advantages of the low temperature system and the high temperature system to produce various products with high current densities. | Lixiao Zhang Shiqing Hu Xuefeng Zhu Weishen Yang | 2017 | Journal of Energy Chemistry2017，26，4： | 6 |
| 9 | Review on the recent progress of laser frontiers in China显示文摘The discussion on laser science and technology during the 'Laser Frontier' forum on 20 to 21 October 2012 in Beijing provided a global overview of laser development status and trends. Several frontiers in the application and industrial development of laser science and technology in China are reviewed. Suggestions on several aspects of China's laser technology and industry development, especially on a number of laser technology fields and their applications that are closely related to the national economy and industry, are proposed. | LIN ZunQi CHEN WeiBiao LOU QiHong FAN Wei XIANG ShiQing XUE HuiBin | 2013 | Science China(Technological Sciences)2013，56，7： | 6 |
| 10 | Doping bioactive elements into a collagen scaffold based on synchronous self-assembly/mineralization for bone tissue engineering显示文摘Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial activities.To obtain collagen with these characteristics,we developed a novel methodology of doping bioactive elements into collagen through the synchronous self-assembly/mineralization(SSM)of collagen.In the SSM model,amorphous mineral nanoparticles(AMN)(amorphous SrCO3,amorphous Ag3PO4,etc.)stabilized by the polyampholyte,carboxymethyl chitosan(CMC),and collagen molecules were the primary components under acidic conditions.As the pH gradually increased,intrafibrillar mineralization occurred via the self-adaptive interaction between the AMNs and the collagen microfibrils,which were self-assembling;the AMNs wrapped around the microfibrils became situated in the gap zones of collagen and finally transformed into crystals.Srdoped collagen scaffolds(Sr-CS)promoted in vitro cell proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells(rBMSCs)and synergistically improved osteogenesis of rBMSCs by altering the macrophage response.Ag-doped collagen scaffolds(Ag-CS)exhibited in vitro antibacterial effects on S.aureus,as well as cell/tissue compatibility.Moreover,Sr-CS implanted into the calvarial defect of a rat resulted in improved bone regeneration.Therefore,the SSM model is a de novo synthetic strategy for doping bioactive elements into collagen,and can be used to fabricate multifunctional collagen scaffolds to meet the clinical challenges of encouraging osteogenesis,boosting the immune response and fighting severe infection in bone defects. | Huanhuan Liu Mingli Lin Xue Liu Ye Zhang Yuyu Luo Yanyun Pang Haitao Chen Dongwang Zhu Xue Zhong Shiqing Ma Yanhong Zhao Qiang Yang Xu Zhang | 2020 | Bioactive Materials2020，5，4： | 6 |
| 11 | Genetic variation and selection of introduced provenances of Siberian Pine(Pinus sibirica) in frigid regions of the Greater Xing'an Range,Northeast China显示文摘Siberian Pine(Pinus sibirica) is an ecologically and economically important species in pristine forests throughout northern Russia. Four provenances of P. sibirica were introduced from Mongolia and Russia to the Greater Xing'an Range(the Daxing'anling), northeast China in 1993. The aim of this research was to study genetic variation and selection of the introduced four Pinus sibirica provenances. Heights(H), basal diameters(BD), survival rates(SR) and crown lengths(CL) of different families were measured as primary outcomes in different growth years. Results of data analyses demonstrated high coefficients of phenotypic variation(PCV) and heritability(H2) for H, BD and CL at 18 years after introduction. PCV and H2 increased with age. Correlations of growth traits between any two growth years were all significantly positive, but the correlation coefficient was smaller when the growth year interval was larger. Correlations between H and the original environment factors decreased gradually, indicating that with long-term subsistence in the new environment, the influence of the source environment declined. Colligation of multiple traits to estimate provenances showed that Novosibirsk, Tomsk, and Altai Mountains had higher survival rates and biomass, and proved more suitable for introduction and plantation in the Greater Xing'an Range in China. | Xiyang Zhao Cheng Wang Shuchun Li Wei Hou Shiqing Zhang Guijie Han Deng Pan Ping Wang Yufei Cheng Guifeng Liu | 2014 | Journal of Forestry Research2014，25，3： | 6 |
| 12 | In situ trapped high-density single metal atoms within graphene: Iron-containing hybrids as representatives for efficient oxygen reduction显示文摘因为他们为特殊催化作用的效率和 chemoselectivity 比传统的催化剂的那些优异,原子地驱散的催化剂在精力变换应用吸引了注意。然而,他们在支持上由于极其低的装载金属的内容有限制,在在高温度的金属地点和数量以及低稳定性的精确控制的困难。我们经由葡萄糖, dicyandiamide,和无机的金属盐的单个步的热热分解准备了一个高度做的单个金属原子混血儿。高角度的环形的黑暗扫描地的传播电子显微镜学(HAADF 茎) 和 X 光检查吸收罚款结构光谱学(XAFS ) 表明做进 graphene 矩阵的氮原子为由产生 metal-Nx 协作结构的金属原子稳定是枢轴的。由于 graphene 矩阵的强壮的抛锚效果,装载内容的金属是在孤立的原子混血儿的超过 4 wt.%( 磅内容象在做磅的混血儿的 9.26 wt.% 一样高) 。而且,单个做铁的混血儿(做 Fe@N 的 graphene ) 为氧减小反应显示出显著 electrocatalytic 表演。山峰力量密度是 199 | Daobin Liu Chuanqiang Wu Shuangming Chen Shiqing Ding Yaofeng Xie Changda Wang Tao Wang Yasir A. Haleem Zia ur Rehman Yuan Sang Qin Liu Xusheng Zheng Yu Wang Binghui Ge Hangxun Xu Li Song | 2018 | Nano Research2018，11，4： | 6 |
| 13 | Labor Market Reform,Income Inequality and Economic Growth in China显示文摘一年 1996 以中国劳动市场改革并且在中国的经济生长模式两个都是一个转弯的点。在 1996 前,劳动市场改革主要通过人的职业和收入结构的调整被实现。自从 1996,雇用重构以雇用地位导致了区别。在以前的阶段的劳动市场改革在工资导致了慢生长,而在后者阶段的改革提高了经济效率。两个阶段使中国经济能使用它低劳动费用的比较优点。劳动市场改革也增加了收入不同,因此,新挑战在支撑经济生长被提出。调整它的发展策略并且介绍劳动的中国需要出售能改进收入平等的改革,以便完成持续经济开发。 | Ming Lu Shiqing Jiang | 2008 | China & World Economy2008，16，6： | 5 |
| 14 | Transition-Metal-Free TEMPO Catalyzed Aerobic Oxidation of Alcohols to Carbonyls Using an Efficient Br2 Equivalent under Mild Conditions显示文摘有效 transition-metal-free 催化系统为白酒的氧气的氧化被开发。使用溴化物使与臬化合联合, H2SO4,和 NaNO2 的催化数量,和面对空气的 2,2,6,6-tetramethylpiperidine N-oxyl 基(速度) ,各种各样的白酒能被变换成相应的醛或酉同类在对在温和条件下面的优秀孤立的收益好。 | Jie Zhang Zengqiang Jiang Dan Zhao Guozhen He Shuangli Zhou Shiqing Han | 2013 | Chinese Journal of Chemistry2013，31，6： | 4 |
| 15 | Influences of Sputtering Angles and Annealing Temperatures on the Magnetic and Magnetostrictive Performances of TbFe Films显示文摘To increase the low-field magnetostriction of TbFe films, the influences of sputtering angles and annealing temperatures on its magnetic and magnetostrictive performances were systematically investigated. With the change in detection of magnetic domains by MFM (magnetic force microscopy) indicates that the easy magnetization direction shifts gradually from perpendicular to parallel to the film plane with decreasing sputtering angles. Annealing can enhance the magnetization and magnetostriction of the TbFe films. However, at too high annealing temperature,both the magnetization and magnetostriction of the TbFe films were suppressed to some extent. | Hongchuan JIANG, Wanli ZHANG, Wenxu ZHANG, Shiqing YANG and Huaiwu ZHANG College of Microelectronics and Solid State Electronics, University of Electronic Science and Technology of China, Chengdu 610054, China | 2005 | Journal of Materials Science & Technology2005，21，3： | 4 |
| 16 | Effect of Li+ ion concentration on upconversion emission and temperature sensing behavior of La2O3:Er3+ phosphors显示文摘The effects of Li^+ co-doping concentration on the structure, upconversion luminescence and temperature sensing behavior of Er^(3+):La_2O_3 phosphors were investigated. X-ray diffraction and scanning electron microscopy observations reveal that Li^+ ion co-doping can change the lattice parameter of La_2O_3 host and increase the particle size of the samples. The optical investigation shows that co-doping of Li^+ ions can enhance the upconversion emission of Er^(3+) ions in La_2O_3 matrix effectively. Most importantly, the temperature sensing sensitivity of the samples is found to be dependent on Li^+ co-doping concentration,when the emission intensity ratio of the(~2H_(11/2)→~4 I_(15/2)) and(~4 S_(3/2)→~4 I_(15/2)) transitions of Er^(3+) is chosen as the thermometric index. Both of the optimum upconversion luminescence and temperature sensing sensitivity are obtained for 7 mol% Li^+ co-doped sample. When the Li^+ concentration is beyond 7 mol%,both the quenching in upconversion intensity and the degradation of temperature sensitivity are observed, which may be due to the serious distortion in local crystal field around Er^(3+) ions caused by the excess Li^+ ions. | Guangrun Chen Ruoshan Lei Shiqing Xu Huanping Wang Shilong Zhao Feifei Huang Yin Tian | 2018 | Journal of Rare Earths2018，36，2： | 4 |
| 17 | Enhanced remediation of Cr(Ⅵ)-contaminated groundwater by coupling electrokinetics with ZVI/Fe_(3)O_(4)/AC-based permeable reactive barrier显示文摘Although widely used in permeation reaction barrier(PRB)for strengthening the removal of various heavy metals,zero-valent iron(ZVI)is limited by various inherent drawbacks,such as easy passivation and poor electron transfer.As a solution,a synergistic system with PRB and electrokinetics(PRB-EK)was established and applied for the efficient removal of Cr(Ⅵ)-contaminated groundwater.As the filling material of PRB,ZVI/Fe_(3)O_(4)/activated carbon(ZVI/Fe_(3)O_(4)/AC)composites were synthesized by ball milling and thermal treatment.A series of continuous flow column experiments and batch tests was conducted to evaluate the removal efficiency of Cr(Ⅵ).Results showed that the removal efficiency of Cr(Ⅵ)remained above 93%even when the bed volume(BV)reached 2000 under the operational parameters(iron/AC mass ratio,2:1;current,5 m A).The mechanism of Cr(Ⅵ)removal by the PRB-EK system was revealed through field emission scanning electron microscopy images,X-ray diffraction,X-ray photoelectron spectroscopy,Fe^(2+) concentration,and redox potential(E h)values.The key in Cr(Ⅵ)reduction was the Fe^(2+)/Fe^(3+) cycle driven by the surface microelectrolysis of the composites.The application of an externally supplied weak direct current maintained the redox process by enhancing the electron transfer capability of the system,thereby prolonging the column lifetime.Cr(Ⅵ)chemical speciation was determined through sequential extraction,verifying the stability and safety of the system.These findings provide a scientific basis for PRB design and the in-situ remediation of Cr(Ⅵ)-contaminated groundwater. | Ruolin Cao Shiqing Liu Xinyu Yang Chunfeng Wang Yanbin Wang Wanfeng Wang Yunqing Pi | 2022 | Journal of Environmental Sciences2022，34，2： | 4 |
| 18 | Monte carlo study on 6 MV photon beams of a CyberKnife stereotactic radiosurgery system显示文摘In this paper,the beam quality and percent depth dose curves for different field sizes of CyberKnife? system were investigated by Monte Carlo simulations using the PENELOPE code,which has been used to simulate 6 MV photon beam.In water phantom,the absolute doses were calculated for Φ10–60 mm collimators,and percent depth dose curves were evaluated for Φ30–60 mm collimators.The agreement of dose distributions of the calculation with measurement was within 3.0%.The mean energy of photon spectrum was 1.46 MeV,and the beam quality index was 0.632,which was slightly smaller than that of measurement. | DONG Xiaoqing LUO Wenyun YUE Kun WANG Chuanshan ZHOU Shiqing PAN Fuxing WANG Enmin WANG Chaozhuang | 2010 | Nuclear Science and Techniques2010，21，1： | 4 |
| 19 | Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane显示文摘Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration;however,the precise mechanism remains unclear.p75 neurotrophin receptor(p75NTR)plays an important role in the regulation of peripheral nerve regeneration.We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75NTRexpression.In this study,we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve.Through RT-PCR and western blot analysis,nerve growth factor-containing fibrin glue membrane significantly increased p75NTRmRNA and protein expression in the Schwann cells at the anastomotic site,in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture.These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75NTRexpression in Schwann cells. | Shengzhong Ma Changliang Peng Shiqing Wu Dongjin Wu Chunzheng Gao | 2013 | Neural Regeneration Research2013，8，36： | 4 |
| 20 | RNA-seq analysis of Paris polyphylla var.yunnanensis roots identified candidate genes for saponin synthesis显示文摘Paris polyphylla Smith var.yunnanensis(Franch.) Hand.-Mazz.is a rhizomatous,herbaceous,perennial plant that has been used for more than a thousand years in traditional Chinese medicine.It is facing extinction due to overharvesting.Steroids are the major therapeutic components in Paris roots,the commercial value of which increases with age.To date,no genomic data on the species have been available.In this study,transcriptome analysis of an 8-year-old root and a 4-year-old root provided insight into the metabolic pathways that generate the steroids.Using Illumina sequencing technology,we generated a high-quality sequence and demonstrated de novo assembly and annotation of genes in the absence of prior genome information.Approximately 87,577 unique sequences,with an average length of 614 bases,were obtained from the root cells.Using bioinformatics methods,we annotated approximately 65.51% of the unique sequences by conducting a similarity search with known genes in the National Center for Biotechnology Information's non-redundant database.The unique transcripts were functionally classified using the Gene Ontology hierarchy and the Kyoto Encyclopedia of Genes and Genomes database.Of 3082 genes that were identified as significantly differentially expressed between roots of different ages,1518(49.25%) were upregulated and 1564(50.75%) were downregulated in the older root.Metabolic pathway analysis predicted that 25 unigenes were responsible for the biosynthesis of the saponins steroids.These data represent a valuable resource for future genomic studies on this endangered species and will be valuable for efforts to genetically engineer P.polyphylla and facilitate saponin-rich plant development. | Tao Liu Xiaoxian Li Shiqing Xie Ling Wang Shengchao Yang | 2016 | Plant Diversity2016，38，3： | 4 |
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