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您的检索式：作者名="Jingkun RAN"
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| 1 | Food resources of the Khog Gzung site on the Tibetan Plateau revealed by sedimentary ancient DNA显示文摘Traditional zooarchaeological and archaeobotanical methods based on morphological identification of the excavated faunal and floral remains have been broadly used in reconstructing ancient subsistence economies. However, the accuracy and reliability of these methods rely heavily on the preservation state of the remains. By sequencing the ancient DNA of plants,animals, and microorganisms preserved in sediment, sedimentary ancient DNA(sedaDNA) now offers a novel approach for reconstructing the taxa composition dated back to hundreds of thousands of years. Yet, its application in open-air archaeological sites is rarely reported. In this study, we attempted to apply sedaDNA shotgun metagenomics on the archaeological deposits of the Khog Gzung site(an open-air site dated to 3160–2954 cal yr BP) on the Tibetan Plateau, and then compared the reconstructed taxonomic composition to the unearthed remains. Results showed that most of the crops and domestic animals identified by the two approaches, such as barley(Hordeum vulgare) and sheep(Ovis aries), are in general consistent. Some species, such as foxtail millet(Setaria italica), however, were only detected by sedaDNA. In addition, a variety of microorganisms were also detected by the sedaDNA. The two approaches combined revealed diversified food resources at the Khog Gzung site, which included crops such as millet, barley and wheat, domestic animals such as sheep and cattle, and likely also wild animals from fishing and hunting. Our data proves that sedaDNA has a great potential in reconstructing the faunal and floral compositions from archaeological deposits, therefore laying the foundation for its border applications. | Zhengquan GU Yu GAO Yiru WANG Jishuai YANG Jingkun RAN Xiaoyan YANG Wangdue SHARGAN Mikkel W.PEDERSEN Guilian SHENG Yucheng WANG Fahu CHEN | 2023 | Science China Earth Sciences2023，66，4： | 0 |
| 2 | An optimized two-step derivatization method for analyzing diethylene glycol ozonation products using gas chromatography and mass spectrometry显示文摘The ozonation of hydroxyl compounds(e.g., sugars and alcohols) gives a broad range of products such as alcohols, aldehydes, ketones, and carboxylic acids. This study developed and optimized a two-step derivatization procedure for analyzing polar products of aldehydes and carboxylic acids from the ozonation of diethylene glycol(DEG) in a non-aqueous environment using gas chromatography–mass spectrometry. Experiments based on Central Composite Design with response surface methodology were carried out to evaluate the effects of derivatization variables and their interactions on the analysis. The most desirable derivatization conditions were reported, i.e., oximation was performed at room temperature overnight with the o-(2,3,4,5,6-pentafluorobenzyl) hydroxyl amine to analyte molar ratio of 6, silylation reaction temperature of 70°C, reaction duration of 70 min, and N,O-bis(trimethylsilyl)-trifluoroacetamide volume of 12.5 μL. The applicability of this optimized procedure was verified by analyzing DEG ozonation products in an ultrafine condensation particle counter simulation system. | Ran Yu Lei Duan Jingkun Jiang Jiming Hao | 2017 | Journal of Environmental Sciences2017，29，3： | 0 |
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