|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis显示文摘AIM:To investigate the utility of immunohistochemical(IHC) staining with an antibody to Mycobacterium tuberculosis(M.tuberculosis) for the diagnosis of intestinal tuberculosis(TB).METHODS:We retrospectively identified 10 patients(4 males and 6 females;mean age = 65.1 ± 13.6 years) with intestinal TB.Clinical characteristics,including age,gender,underlying disease,and symptoms were obtained.Chest radiograph and laboratory tests,including sputum Ziehl-Neelsen(ZN) staining,M.tuberculosis culture,and sputum polymerase chain reaction(PCR) for tubercle bacilli DNA,as well as Tuberculin skin test(TST) and QuantiFERON-TB gold test(QFT),were examined.Colonoscopic records recorded on the basis of Sato's classification were also reviewed,in addition to data from intestinal biopsies examined for histopathological findings,including hematoxylin and eosin staining,and ZN staining,as well as M.tuberculosis culture,and PCR for tubercle bacilli DNA.For the present study,archived formalin-fixed paraffin-embedded(FFPE) intestinal tissue samples were immunohistochemically stained using a commercially available species-specific monoclonal antibody to the 38-kDa antigen of the M.tuberculosis complex.These sections were also stained with the pan-macrophage marker CD68 antibody.RESULTS:From the clinical data,we found that no patients were immunocompromised,and that the main symptoms were diarrhea and weight loss.Three patients displayed active pulmonary TB,six patients(60%) had a positive TST,and 4 patients(40%) had a positive QFT.Colonoscopic findings revealed that all patients had type 1 findings(linear ulcers in a circumferential arrangement or linear ulcers arranged circumferentially with mucosa showing multiple nodules),all of which were located in the right hemicolon and/or terminal ileum.Seven patients(70%) had concomitant healed lesions in the ileocecal area.No acid-fast bacilli were detected with ZN staining of the intestinal tissue samples,and both M.tuberculosis culture and PCR for tubercle bacilli DNA were negative in all samples.The histopathological data revealed that tuberculous granulomas were present in 4 cases(40%).IHC staining in archived FFPE samples with anti-M.tuberculosis monoclonal antibody revealed positive findings in 4 patients(40%);the same patients in which granulomas were detected by hematoxylin and eosin staining.M.tuberculosis antigens were found to be mostly intracellular,granular in pattern,and primarily located in the CD68 + macrophages of the granulomas.CONCLUSION:IHC staining with a monoclonal antibody to M.tuberculosis may be an efficient and simple diagnostic tool in addition to classic examination methods for the diagnosis of intestinal TB. | Yasushi Ihama Akira Hokama Kenji Hibiya Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Haley L Cash Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2012 | World Journal of Gastroenterology2012，18，47： | 12 |
| 2 | Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010显示文摘 | Christopher J L Murray Theo Vos Rafael Lozano Mohsen Naghavi Abraham D Flaxman Catherine Michaud Majid Ezzati Kenji Shibuya Joshua A Salomon Safa Abdalla Victor Aboyans Jerry Abraham Ilana Ackerman Rakesh Aggarwal Stephanie Y Ahn Mohammed K Ali Mohammad A | 2012 | The Lancet . 2012 (9859)2012，，9859： | 2 |
| 3 | Generation of planar and helical elliptical gears by application of rack-cutter, hob, and shaper 显示文摘 | Litvin F L Ignacio G P Kenji Y | 2007 | Comput Methods Appl Mech Engrg2007，196，： | 1 |
| 4 | Determination of temperature recovery time in differential-pressure-based air leak detector显示文摘 | L G Harus Maolin Cai Kenji Kawashima | 2006 | Measurement Science and Technology2006，，17： | 1 |
| 5 | Determination of Temperature Recovery Time in Differential-Pressure-Based Air Leak Detector显示文摘 | Harus L G Cai Maolin Kawashima Kenji | 2006 | Measurement Science and Technology2006，，： | 1 |
| 6 | Optimum phase matching and effective nonlinear coefficients of LBO and KTP for continuously changing wavelength显示文摘 | Liu L Q Kenji Nagashima | 1999 | Optics and Laser Technology1999，，31： | 1 |
| 7 | Sweet cucurbitane-glycosides from fruits of Siraitia siamensis(chi-ziluo-han-guo):A Chinese folk medicine显示文摘 | RYOJI K NIE R L KENJI Nt | 1989 | Agric Biol Chem1989，53，12： | 1 |
| 8 | Formic acid directly assisted solid-state synthesis of metallic catalysts without further reduction: as-prepared CulZnO catalysts for low-temperature methanol synthesis显示文摘 | Shi L Shen W Z Yang G H Fan X J Jin Y Z Zeng C Y Kenji Matsuda Tsubaki Noritatsu | 2013 | Journal of Catalysis2013，302，： | 1 |
| 9 | Dysplastic nodules frequently develop into hepatocellular carcinoma in patients with chronic viral hepatitis and cirrhosis显示文摘 | Masahiro k Kenji l Tetsuya h | 2005 | American Cancer Soclety2005，12，： | 1 |
| 10 | Isolation of an antiviral polysaccharide nostoflan from a terrestrial cyanobacterium Nostoc flagelliforme 显示文摘 | KENJI K JUNG-BUM L KYOKO H | 2005 | Journal of Natural Products2005，68，7： | 1 |
| 11 | Structure-activity relationship for inhibition of 5a-reductase by triterpenoids isolated fromGanoderma lucidum显示文摘 | JIE L KENJI K KUNIYOSHI S | 2006 | Bioorg Med Chem2006，14，： | 1 |
| 12 | Design, Simulation of Meshing, and Contact Stresses for an Improved Worm Gear Drive 显示文摘 | LITVIN Faydor L GONZALEZ-PEREZ Ignacio YUKISHIMA Kenji | 2007 | Mechanism and Machine Theory2007，42，： | 1 |
| 13 | Dysplastic nodul- ers frequently develop into hepatocellular carcinoma in patients with chronic viral hepatitis and cirrhosis 显示文摘 | MASABIRO K KENJI L TETSUYA H | 2006 | Cancer2006，3，： | 1 |
| 14 | Expression of biologically active recombinant equine interferon-γ by expression systems using insect cells and silkworm larvae显示文摘 | Donglai WU Kenji M Nihong L | 2002 | Cytokine2002，20，21： | 1 |
| 15 | A novel antimicrobial substance in etiolated seedlings of lay显示文摘 | YUKIO L KENJI O HIROSHI R | 1993 | Biosci Biotechnol Biochem1993，57，5： | 1 |
| 16 | Shrinking thermokarst ponds and groundwater dynamics in discontinuous permafrost near council, Alaska显示文摘 | Kenji Y Hinzman L D | 2003 | Permafrost and Periglacial Processes2003，14，： | 1 |
| 17 | Generation of Planar and Helical Elliptical Gears by Application of Rackcutter, Hob, and Shaper显示文摘 | FAYDOR L L IGNACIO G P KENJI Y | 2007 | Computer Methods in Applied Mechanics and Engineering2007，196，4144： | 1 |
| 18 | Structure- activity relationship for inhibition of 5a-reductase by triterpenoids isolated from Ganoderma lucidum 显示文摘 | JIE L KENJI K KUNIYOSHI S | 2006 | Bioorg Med Chem2006，14，： | 1 |
| 19 | Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization显示文摘 | Srikumar S Kenji O Alexander L | 2003 | J Clin Microbiol2003，41，10： | 1 |
| 20 | SIZi-Mediated Sumoylafion of ICE1 Controls CBF3/DREB1A Expression and Freezing Tolerance in Arabidopsis显示文摘 | Kenji M Jing B J Jiyoung L | 2007 | Cell2007，19，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员