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| 1 | 几种常用保鲜方法对草莓保鲜效果的研究显示文摘通过对6种(冷激、氯化钙、植酸、过氧乙酸、低温、壳聚糖涂膜)常用草莓保鲜方法的比较试验,分析各种保鲜方法对草莓贮藏期的失重率、腐烂率、VC含量、可溶性糖含量、可滴定酸含量及可溶性固形物含量的影响效应。研究结果表明,各处理均能明显减缓草莓果实的VC、可溶性糖、可滴定酸和可溶性固形物含量的下降,抑制草莓腐烂和失水,对草莓保鲜有一定的效果。其中冷激处理综合保鲜效果较优,且操作简单,成本较低,最适合普通农户贮藏保鲜草莓。 | 王纪忠 张绍铃 周青 陶书田 Shahrokh Khanizadeh 叶玉秀 | 2012 | 食品研究与开发2012，33，1： | 24 |
| 2 | 存放方式对壳聚糖涂膜草莓保鲜效果的影响显示文摘[目的]研究提高壳聚糖保鲜草莓的效果。[方法]以新鲜草莓为材料,用不同浓度的壳聚糖溶液涂膜并进行密封与未密封处理。通过测定不同存放时间后草莓腐烂率、失重率、可溶性固形物含量、可滴定酸含量、VC含量的变化来探讨不同浓度壳聚糖涂膜后存放方式对草莓保鲜效果的影响。[结果]1.0%壳聚糖涂膜密封存放最有利于草莓保鲜,其抑制草莓腐烂率、失重率的下降,延缓了可溶性固形物含量、可滴定酸含量、VC含量下降的效果明显好于其他处理。[结论]壳聚糖涂膜后采用密封存放可提高对草莓的保鲜效果。 | 周青 王纪忠 陶书田 张绍铃 Shahrokh Khanizadeh 叶玉秀 | 2011 | 食品工业科技2011，32，8： | 8 |
| 3 | ‘砀山酥梨’果实CCoAOMT基因的克隆与表达分析显示文摘[目的]通过克隆‘砀山酥梨’(Pyrus bretschneideri ‘Dangshansuli’)果实中咖啡酰-辅酶AO-甲基转移酶(CCoAOMT)基因的全长序列,并对其进行生物信息学及基因表达差异分析,阐释了其在梨果实木质素合成途径的作用,以期为今后利用基因调控技术来改变果实中石细胞含量从而改善梨果实品质提供一定的理论依据。[方法]以15年生‘砀山酥梨’果实为试材,根据从NCBI数据库中搜索得到关于植物木质化的CCoAOMT基因序列与梨基因组数据库调取的序列比对,利用RT-PCR技术克隆得到了木质素生物合成途径中的一种关键酶基因PbCCoAOMT,GenBank登录号为KJ577544。[结果]该基因全长1133bp,具有1个744bp的完整开放阅读框(ORF),编码247个氨基酸。序列比对及系统进化树分析表明:CCoAOMT酶是氧甲基转移酶类,与其他植物CCoAOMT编码的蛋白序列有较高的相似性,尤其与枇杷的相似度最高,达到98.79%,且亲缘关系最近。利用荧光定量PCR技术对其在果实不同发育时期表达模式的分析发现,基因表达量呈先上升后下降的趋势,与梨果实中石细胞含量的变化趋势相似。[结论]初步认为从‘砀山酥梨’中克隆得到的PbCCoAOMT基因可能参与了梨果实中木质素的代谢。 | 王丹阳 高付永 孙炜 芮伟康 Khanizadeh Shahrokh 张绍铃 陶书田 | 2015 | 南京农业大学学报2015，38，1： | 6 |
| 4 | 梨果实中肉桂醇脱氢酶基因的克隆与序列分析显示文摘肉桂醇脱氢酶(CAD)是木质素生物合成过程中的一个关键酶。本研究分别提取了'砀山酥梨'及'幸水'梨果肉总RNA,并通过RT-PCR、克隆和测序,成功获得了2个CAD的大小为689bp的cDNA片段,并分别命名为PB-CAD(登录号:FJ478151)和PP-CAD(登录号:FJ478152)。在核苷酸水平上,这两个基因片段只有4个碱基的差异,编码相似性达99.6%的两个氨基酸序列,该序列由229个残基组成。通过蛋白质序列比较,发现梨果实CAD与许多植物中CAD氨基酸序列极为相似,与苹果Malus domestica(AAC06319)、梅Prunus mume(BAE48658)、枇杷Eriobotryajaponica(ABV44810)和葡萄Vitis vinifera(CAO21890)的相似性分别达到97.8%、95%、92.6%和83.4%。 | 陶书田 赵梅 张彪 Khanizadeh Shahrokh 张绍铃 | 2012 | 中国南方果树2012，41，3： | 3 |
| 5 | PbMC1a/1b regulates lignification during stone cell development in pear(Pyrus bretschneideri)fruit显示文摘Programmed cell death(PCD)and secondary cell wall(SCW)thickening in pear fruit are accompanied by the deposition of cellulose and lignin to form stone cells.Metacaspase is an important protease for development,tissue renewal and PCD.The understanding of the molecular mechanism whereby pear(Pyrus)metacaspase promotes PCD and cell wall lignification is still limited.In this study,the Metacaspases gene family(PbMCs)from P.bretschneideri was identified.PbMC1a/1b was associated with lignin deposition and stone cell formation by physiological data,semiquantitative real-time polymerase chain reaction(RT-PCR)and quantitative RT-PCR(qRT-PCR).Relative to wildtype(WT)Arabidopsis,the overexpression of PbMC1a/1b increased lignin deposition and delayed growth,thickened the cell walls of vessels,xylary fibers and interfascicular fibers,and increased the expression of lignin biosynthetic genes.Yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and GST pull-down assays indicated that the PbMC1a/1b protein physically interacted with PbRD21.Simultaneously,the transient expression of PbMC1a/1b and PbRD21 led to significant changes in the expression of genes and lignin contents in pear fruits and flesh calli.These results indicate that PbMC1a/1b plays an important role in cell wall lignification,possibly by interacting with PbRD21 to increase the mRNA levels of some lignin synthesis-associated genes and promote the formation of stone cells in pear fruit. | Xin Gong Zhihua Xie Kaijie Qi Liangyi Zhao Yazhou Yuan Jiahui Xu Weikang Rui Katsuhiro Shiratake Jianping Bao Shahrokh Khanizadeh Shaoling Zhang Shutian Tao | 2020 | Horticulture Research2020，7，1： | 3 |
| 6 | Polyphenol composition and total antioxidant capacity of selected apple genotypes for processing显示文摘 | Shahrokh Khanizadeh Rong Tsao Djamila Rekika | 2008 | Journal of Food Composition and Analysis2008，21，： | 1 |
| 7 | Assessing chilling tolerance in roses chlorophyll fluorescence显示文摘 | Hakam P Khanizadeh S DeEll J R | 2000 | HortScience2000，35，： | 1 |
| 8 | Use of response surface methodology in shelf life extension studies of a bakery product显示文摘 | SMITH J P KHANIZADEH S VAN DE VOORT F R | 1988 | Food Microbiology1988，5，3： | 1 |
| 9 | Breeding strawberries for Eastern Central Canada显示文摘 | Shahrokh Khanizadeh | 1994 | Euphytica (-)1994，，1： | 1 |
| 10 | Anatomy,ultrastructure and lignin distribution of stone cells in two Pyrus species显示文摘 | Tao S T Khanizadeh S Zhang H | 2009 | Plant Science2009，176，3： | 1 |
| 11 | Polyphenol composition and total antioxidant capacity of selected apple genotypes for processing 显示文摘 | Khanizadeh S Tsao R Rekika D | 2008 | Joumal ofComposition andAnalysis2008，，21： | 1 |
| 12 | 长货架期树莓高代品系特性及主要性状相关性显示文摘试验测定了树莓不同品系果实产量,并对与货架期相关的果实品质及主要性状进行了相关性分析。结果表明,SJR941-18,SJR941-1和SJR944-2是果实较大的品种。Boyne,Festival,Killarney和SJR941-5茎的耐寒性显著优于其他品种,使这些品种有较高的产量。树莓果实产量与茎的活力和健康花芽数呈显著正相关,与死亡茎长度呈显著负相关。茎的活力与健康花芽数、健康茎长度呈显著正相关。果实硬度和可溶物含量、果实表面绒毛多少与解冻后果汁百分比亦有显著相关性。 | 王石宝 Shahrokh Khanizadeh | 2005 | 山西农业科学2005，33，3： | 1 |
| 13 | Inter sim- ple sequence repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes 显示文摘 | Debnath S C Khanizadeh S Jamieson A R | 2008 | Canadian journal of plant science2008，88，： | 1 |
| 14 | Use of chlorophyll fluorescence to evaluate chilling tolerance in strawberry plants显示文摘 | Khanizadeh S Deeii J Hakam N | | 0，，538： | 1 |
| 15 | Anatomy, ultra- structure and lignin distribution of stone ceLLs in two Pyrus species 显示文摘 | Tao S Khanizadeh S Zhang H | 2009 | PLant Science2009，176，： | 1 |
| 16 | Seasonal variation of hydrophilu,hydrophobic , and charged amino acids in developing apple flower buds显示文摘 | KHANIZADEHS BUSZARD D ZARKADAS C G | 1994 | J Plant Nutrition1994，17，11： | 1 |
| 17 | Anatomy, ultrastructure and lignin distribution of stone cells in two Pyrus species显示文摘 | Tao S Khanizadeh S Zhang H | 2009 | Plant Science2009，176，3： | 1 |
| 18 | Roseberry and Rosalyne: Two new hardy, day-neutral, red flowering strawberry cultivars显示文摘 | Khanizadeh S Cousineau J Deschines M | 2002 | Acta Horticulturae2002，567，1： | 1 |
| 19 | Anatomy, uhrastructure and lignin distribution of stone cell in two Pyrus species 显示文摘 | Tao S Khanizadeh S Zhang H | 2009 | Plant Science2009，176，3： | 1 |
| 20 | Anatomy, ultrastruc- ture and lignin distribution of stone ceils in two Pyrus species 显示文摘 | Tao S Khanizadeh S Zhang H | 2009 | Plant Science2009，176，： | 1 |
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