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| 1 | Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus显示文摘Dysregulated B-cell activation plays pivotal roles in systemic lupus erythematosus(SLE),which makes B-cell depletion a potential strategy for SLE treatment.The clinical success of anti-CD19 CAR-T cells in treating B-cell malignancies has attracted the attention of researchers.In this study,we aimed to investigate the feasibility of applying anti-CD19 CAR-T cell therapy to SLE treatment in a mouse disease model.We constructed murine anti-CD19 CARs with either CD28 or 4-1BB as the intracellular costimulatory motif and evaluated the therapeutic function of the corresponding CAR-T cells by infusing them into MRL-lpr mice.Furthermore,anti-CD19 CAR-T cells were transferred to MRL-lpr mice before the onset of disease to determine their role in SLE prevention.According to our observations,compared with antibody treatment,the adoptive transfer of our anti-CD19 CAR-T cells showed a more sustained B-cell-depletion effect in MRL-lpr mice.The transfer of syngeneic anti-CD19 CAR-T cells not only prevented disease pathogenesis before the onset of disease symptoms but also displayed therapeutic benefits at a later stage after disease progression.We also tried to optimize the treatment strategy and found that compared with CAR-T cells with the CD28 costimulatory motif,CAR-T cells with the 4-1BB costimulatory motif showed better therapeutic efficiency without cell enrichment.Taken together,these results show that anti-CD19 CAR-T cell therapy was effective in the prevention and treatment of a murine model of SLE,indicating its potential for clinical use in patients. | Xuexiao Jin Qin Xu Chengfei Pu Kaixiang Zhu Cheng Lu Yu Jiang Lei Xiao Yongmei Han Linrong Lu | 2021 | Cellular & Molecular Immunology2021，18，8： | 12 |
| 2 | Priming of NLRP3 inflammasome activation by Msn kinase MINK1 in macrophages显示文摘The nucleotide-binding domain,leucine-rich-repeat containing family,pyrin domain-containing 3(NLRP3)inflammasome is essential in inflammation and inflammatory disorders.Phosphorylation at various sites on NLRP3 differentially regulates inflammasome activation.The Ser725 phosphorylation site on NLRP3 is depicted in multiple inflammasome activation scenarios,but the importance and regulation of this site has not been clarified.The present study revealed that the phosphorylation of Ser725 was an essential step for the priming of the NLRP3 inflammasome in macrophages.We also showed that Ser725 was directly phosphorylated by misshapen(Msn)/NIK-related kinase 1(MINK1),depending on the direct interaction between MINK1 and the NLRP3 LRR domain.MINK1 deficiency reduced NLRP3 activation and suppressed inflammatory responses in mouse models of acute sepsis and peritonitis.Reactive oxygen species(ROS)upregulated the kinase activity of MINK1 and subsequently promoted inflammasome priming via NLRP3 Ser725 phosphorylation.Eliminating ROS suppressed NLRP3 activation and reduced sepsis and peritonitis symptoms in a MINK1-dependent manner.Altogether,our study reveals a direct regulation of the NLRP3 inflammasome by Msn family kinase MINK1 and suggests that modulation of MINK1 activity is a potential intervention strategy for inflammasomerelated diseases. | Kaixiang Zhu Xuexiao Jin Zhexu Chi Sheng Chen Songquan Wu Richard D.Sloan Xuai Lin Dante Neculai Di Wang Hu Hu Linrong Lu | 2021 | Cellular & Molecular Immunology2021，18，10： | 2 |
| 3 | Dynamic changes in physicochemical property,biogenic amines content and microbial diversity during the fermentation of Sanchuan ham显示文摘Sanchuan ham is appreciated in Yunnan Province,China,for its characteristic flavor and taste,while the microbial community structure and biogenic amines content remain unclear during fermentation processes.In this study,we explored the physicochemical property,biogenic amines concentration and microbial diversity of external and internal Sanchuan ham by high-throughput sequencing during the processing of Sanchuan ham.Results showed that the nitrite remained at a stable level of 0.15 mg/kg which was significantly lower than the national health standard safety level of 20 mg/kg.In addition,compared with fresh hams,the content of total free amino acids in ripe Sanchuan ham has grown 14 folds;sour and bitter were the main tastes of Sanchuan ham.Notably,the concentration of cadaverine was the highest of all biogenic amines during the entire fermentation period.At the bacterial phyla level,Firmicutes and Actinobacteria were the two main phyla,while at the genus level,Staphylococcus was a significant strain throughout the whole fermentation.Moreover,the dry stage has a great impact on the succession change of microbial community structure.Simultaneously,the change trends and composition of bacteria in the interior have slight discrepancies with those of the exterior of Sanchuan ham. | Zhongai Chen Hong Chen Huan Du Cong Chen Kaixiang Lu Qiaoli Xue Yongjin Hu | 2024 | Food Science and Human Wellness2024，13，1： | 0 |
| 4 | Constraining self-interacting dark matter with the full dataset of PandaX-II显示文摘Self-interacting dark matter(SIDM)is a leading candidate proposed to solve discrepancies between predictions of the prevailing cold dark matter theory and observations of galaxies.Many SIDM models predict the existence of a light force carrier that mediates strong dark matter self-interactions.If the mediator couples to the standard model particles,it could produce characteristic signals in dark matter direct detection experiments.We report searches for signals of SIDM models with a light mediator using the full dataset of the PandaX-II experiment,basing on a total exposure of 132 tonne-days.No significant excess over background is found,and our likelihood analysis leads to a strong upper limit on the dark matter-nucleon coupling strength.We further combine the PandaX-II constraints and those from observations of the light element abundances in the early universe,and show that direct detection and cosmological probes can provide complementary constraints on dark matter models with a light mediator. | Jijun Yang Abdusalam Abdukerim Wei Chen Xun Chen Yunhua Chen Chen Cheng Xiangyi Cui Yingjie Fan Deqing Fang Changbo Fu Mengting Fu Lisheng Geng Karl Giboni Linhui Gu Xuyuan Guo Ke Han Changda He Shengming He Di Huang Yan Huang Ran Huo Yanlin Huang Zhou Huang Xiangdong Ji Yonglin Ju Shuaijie Li Qing Lin Huaxuan Liu Jianglai Liu Xiaoying Lu Wenbo Ma Yugang Ma Yajun Mao Yue Meng Nasir Shaheed Kaixiang Ni Jinhua Ning Xuyang Ning Xiangxiang Ren Changsong Shang Guofang Shen Lin Si Andi Tan Anqing Wang Hongwei Wang Meng Wang QiuHong Wang Siguang Wang Wei Wang Xiuli Wang Zhou Wang Mengmeng Wu Shiyong Wu Weihao Wu Jingkai Xia Mengjiao Xiao Xiang Xiao Pengwei Xie Binbin Yan Yong Yang Chunxu Yu Hai-Bo Yu Jumin Yuan Ying Yuan Xinning Zeng Dan Zhang Tao Zhang Li Zhao Qibin Zheng Jifang Zhou Ning Zhou Xiaopeng Zhou | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，11： | 0 |
| 5 | Defect Engineering in g‑C_(3)N_(4)Quantum‑Dot‑Modified TiO_(2)Nanofiber:Uncovering Novel Mechanisms for the Degradation of Tetracycline in Coexistence with Cu^(2+)显示文摘Herein,g-C_(3)N_(4)quantum-dot-modified TiO_(2)nanofibers were fabricated and used as an efficient photocatalyst for the investigation of the influence of Cu^(2+)and the interaction mechanism between Cu^(2+)and surface defects in tetracycline degradation.Results showed that the effect of Cu^(2+)switched from promoting to inhibiting the tetracycline degradation as the amount of Cu^(2+)accumulated on the catalyst surface increased.The introduction of surface defects can prevent the inhibiting effect of Cu^(2+),resulting in the more complete degradation of tetracycline in contrast to the non-defective sample.Theoretical calculations further revealed that the defects can be used to tune the conduction band of the composite,inducing the reduction reaction of Cu^(2+)and inhibiting the accumulation of Cu on the surface of catalysts.Moreover,the Cu introduced to the catalyst surface provided new active sites,thereby promoting photocatalytic degradation.These findings provide new insights into the design of advanced fiber materials for water purification in complex environments. | Jingzhe Zhang Xin Wang Kaixiang Shen Wenjing Lu Jun Wang Fuming Chen | 2023 | Advanced Fiber Materials2023，5，1： | 0 |
| 6 | Computational fluid dynamics-discrete element method simulation of stirred tank reactor for graphene production显示文摘Liquid phase exfoliation(LPE)process for graphene production is usually carried out in stirred tank reactor and the interactions between the solvent and the graphite particles are important as to improve the production efficiency.In this paper,these interactions were revealed by computational fluid dynamics–discrete element method(CFD-DEM)method.Based on simulation results,both liquid phase flow hydrodynamics and particle motion behavior have been analyzed,which gave the general information of the multiphase flow behavior inside the stirred tank reactor as to graphene production.By calculating the threshold at the beginning of graphite exfoliation process,the shear force from the slip velocity was determined as the active force.These results can support the optimization of the graphene production process. | Shuaishuai Zhou Jing Li Kaixiang Pang Chunxi Lu Feng Zhu Congzhen Qiao Yajie Tian Jingwei Zhang | 2023 | Chinese Journal of Chemical Engineering2023，64，12： | 0 |
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