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| 1 | Cycloastragenol upregulates SIRT1 expression,attenuates apoptosis and suppresses neuroinflammation after brain ischemia显示文摘Cycloastragenol(CAG)is the active form of astragaloside V isolated from Astragalus Radix,which displays multiple pharmacological effects.Silent information regulator 1(SIRT1),a classll histone deacetylase,has been shown to play an important role in neuroprotection against cerebral ischemia.In this study,we investigated whether CAG protected against ischemic brain injury and,if so,whether the beneficial effects were associated with the regulation of SIRT1 in the ischemic brain.Mice were subjected to 45 min of middle cerebral artery occlusion(MCAO)followed by reperfusion.CAG(5,10,20 mg/kg)was injected intraperitoneally at the onset of reperfusion,12h later and then twice daily for up to three days.CAG dose-dependently reduced brain infarct volume,significantly ameliorated functional deficits,and prevented neuronal cell oss in MCAO mice.Meanwhile,CAG signicantly reduced matrix metalloproteinase-9 activity,prevented tight junction degradation and subsequently ameliorated blood-brain barier disruption.Moreover,CAG significantly upregulated SIRT1 expression in the ischemic brain but did not directly activate its enzymatic activity.Concomitant with SIRT1 upregulation,CAG reduced p53 acetylation and the ratio of Bax to Bcl-2 in the ischemic brain.CAG also inhibited NF-κB p65 nuclear translocation.As a result,CAG suppressed the mRNA expression of pro-inflammatory cytokines,including TNF-α and IL-1β,and inhibited the activation of microglia and astrocytes in the ischemic brain.Our findings suggest that CAG is neuroprotective against ischemic brain injury in mice and that its beneficial effect may involve SIRT1 upregulation and the inhibition of apoptosis and neuroinflammation in the ischemic brain. | Man Li Shichun Li Bao-kai Dou Yving-xiang Zou Hao-zhen Han Dong-xiang Liu Zunji Ke Zhi-fei Wang | 2020 | Acta Pharmacologica Sinica2020，41，8： | 22 |
| 2 | Metformin promotes microglial cells to facilitate myelin debris clearance and accelerate nerve repairment after spinal cord injury显示文摘Spinal cord injury(SCI)is one kind of severe trauma for central nervous system.Myelin debris clearance and axon regeneration are essential for nerve regeneration after SCI.Metformin,a glucose-lowering drug,has been demonstrated to promote the locomotor functional recovery after SCI.In this study,we investigated the role and molecular mechanism of metformin on myelin preservation in a rat SCI model.SCI was induced in rats by compression at T9 level using a vascular clip.We showed that administration of metformin(50 mg·kg^(-1)·d^(-1),ip)for 28 days significantly improved locomotor function in SCI rats.Metformin also ameliorated SCI-induced neuronal apoptosis and promoted axon regeneration in the spinal cord.Using co-immunofluorescence of IBa-1 and MBP,and luxol fasting blue(LFB)staining,we demonstrated that metformin promoted the transformation of M1 to M2 phenotype polarization of microglial cells,then greatly facilitated myelin debris clearance and protected the myelin in SCI rats.Furthermore,metformin ameliorated SCI-induced blockade of autophagic flux in the spinal cord,and enhanced the fusion of autophagosome and lysosome by inhibiting the AMPK-mTOR signaling pathway.Moreover,metformin significantly attenuated inflammatory responses in the spinal cord.In LPS-treated BV2 cells,pretreatment with metformin(2 mM)significantly enhanced autophagy level,suppressed inflammation and cell apoptosis.The protective effects were blocked in the presence of an autophagy inhibitor 3-methyladenine(3-MA,5 mM),suggesting that the effect of metformin on autophagy in microglial cells is essential for the myelin preservation during nerve recovery.This study reveals a novel therapeutic effect of metformin in SCI recovery by regulating the activation of microglial cells and enhancing its autophagy level. | Yan-qing Wu Jun Xiong Zi-li He Yuan Yuan Bei-ni Wang Jing-yu Xu Man Wu Su-su Zhang Shu-fang Cai Jia-xin Zhao Ke Xu Hong-yu Zhang Jian Xiao | 2022 | Acta Pharmacologica Sinica2022，43，6： | 8 |
| 3 | Excavation of underground research laboratory ramp in granite using tunnel boring machine: Feasibility study显示文摘Underground research laboratory(URL)plays an important role in safe disposal of high-level radioactive waste(HLW).At present,the Xinchang site,located in Gansu Province of China,has been selected as the final site for China’s first URL,named Beishan URL.For this,a preliminary design of the Beishan URL has been proposed,including one spiral ramp,three shafts and two experimental levels.With advantages of fast advancing and limited disturbance to surrounding rock mass,the tunnel boring machine(TBM)method could be one of the excavation methods considered for the URL ramp.This paper introduces the feasibility study on using TBM to excavation of the Beishan URL ramp.The technical challenges for using TBM in Beishan URL are identified on the base of geological condition and specific layout of the spiral ramp.Then,the technical feasibility study on the specific issues,i.e.extremely hard rock mass,high abrasiveness,TBM operation,muck transportation,water drainage and material transportation,is investigated.This study demonstrates that TBM technology is a feasible method for the Beishan URL excavation.The results can also provide a reference for the design and construction of HLW disposal engineering in similar geological conditions.2020 Institute of Rock and Soil Mechanics,Chinese Academy of Sciences.Production and hosting by Elsevier B.V.This is an open access article under the CC BY-NC-ND license(http://gffzzeb6f7f563f66445dho0k5xonow55u65fv.ffgz.tsg.suse.edu.cn/licenses/by-nc-nd/4.0/). | Hongsu Ma Ju Wang Ke Man Liang Chen Qiuming Gong Xingguang Zhao | 2020 | Journal of Rock Mechanics and Geotechnical Engineering2020，12，6： | 6 |
| 4 | Progress of circulating fluidized bed combustion technology in China:a review显示文摘Circulating fluidized bed(CFB)technology plays an important role in the utilization of low-grade coal in China.This article reviews CFB combustion technology development in China and summarizes recent achievements.Since 1990 Chinese engineers and researchers have been undertaking work to improve CFB boiler technology.A completely novel CFB boiler design theory was developed and used in the domestic manufacturing of CFB boilers with various capacities.China is the largest supplier and customer of CFB boilers in the world due to the widespread use of CFB boilers.In 2007,the lower energy consumption CFB technology was successfully developed by re-specifying the fluidization state,which reduced the power consumption of forced fans and solved the potential erosion problems on the water wall.Afterwards,in order to increase the electric power generation efficiency,the supercritical CFB(SCCFB)boiler was developed and the first 600 MW SCCFB boiler was demonstrated and put into commercial operation in 2013.The success of the technology is evident with over 80 SCCFB boilers on order with capacities of 350 MW to 660 MW.Chinese scientists and engineers are also developing technology to lower the emissions of CFB combustion to meet the requirements of China’s strict emission regulations.This emission reduction is through high-efficiency desulfurization by limestone injection into the furnace and low NOx combustion,ultra-low emission of SO2 and NOx in the furnace can be realized by improving the bed quality and increasing the solid circulation rate.There is currently further research and design development being undertaken to develop a 660 MW ultra-supercritical CFB(USCCFB)boiler.The new boiler is expected to be operational before 2020 resulting in higher efficiency and lower energy consumption and emissions. | Runxia Cai Xiwei Ke Junfu Lyu Hairui Yang Man Zhang Guangxi Yue Wen Ling | 2017 | Clean Energy2017，1，1： | 5 |
| 5 | Numerical study of the semi-circular bend dynamic fracture toughness test using discrete element models显示文摘The semi-circular bend(SCB) dynamic fracture toughness test is simulated using discrete element models. The influence of the frictional boundary condition, constitutive law and specimen thickness on the test measurements is investigated. It is found that friction between loading plates and the rock specimen affects the test results. Therefore, friction must be carefully considered to obtain accurate measurements. The simulation results also show that in contrast to the 2D model in which a rate-dependent cohesive law must be introduced, 3D models with a rate-independent law can produce good results. Furthermore, the study suggests that test measurements are seriously affected by specimen thickness; thus, full 3D modeling is required for simulation of the SCB test. | ZHAO GaoFeng KAZERANI Tohid MAN Ke GAO Mz ZHAO Jian | 2015 | Science China(Technological Sciences)2015，58，9： | 4 |
| 6 | Synthesis and Characterization of a Novel Compound SnDy2O4显示文摘A new phase, the rare earth complex oxide SnDy2O4 was synthesized by the thermal decomposition of its oxalate precursor that was prepared by rheological phase reaction method. TG, IR, XRD and EPS were used to prove the formation of the compound SnDy2O4. The structure of SnDy2O4 was refined by Rietveld analysis. SnDy2O4 is cubic, a = 7.40366 ? V = 405.82 ?, Z = 4. | Yong ZHANG, Ke Li ZHANG*, Man Ke JIA, Hao TANG, Ju Tang SUN, Liang Jie YUAN College of Chemistry and Molecular science, Department of Chemistry, Wuhan University, Wuhan 430072 | 2002 | Chinese Chemical Letters2002，13，6： | 4 |
| 7 | UBE2S interacting with TRIM28 in the nucleus accelerates cell cycle by ubiquitination of p27 to promote hepatocellular carcinoma development显示文摘Genomic sequencing analysis of tumors provides potential molecular therapeutic targets for precision medicine.However,identifying a key driver gene or mutation that can be used for hepatocellular carcinoma(HCC)treatment remains difficult.Here,we performed whole-exome sequencing on genomic DNA obtained from six pairs of HCC and adjacent tissues and identified two novel somatic mutations of UBE2S(p.Gly57Ala and p.Lys63Asn).Predictions of the functional effects of the mutations showed that two amino-acid substitutions were potentially deleterious.Further,we observed that wild-type UBE2S,especially in the nucleus,was significantly higher in HCC tissues than that in adjacent tissues and closely related to the clinicopathological features of patients with HCC.Functional assays revealed that overexpression of UBE2S promoted the proliferation,invasion,metastasis,and G1/S phase transition of HCC cells in vitro,and promoted the tumor growth significantly in vivo.Mechanistically,UBE2S interacted with TR1M28 in the nucleus,both together enhanced the ubiquitination of p27 to facilitate its degradation and cell cycle progression.Most importantly,the small-molecule cephalomannine was found by a luciferase-based sensitive high-throughput screen(HTS)to inhibit UBE2S expression and significantly attenuate HCC progression in vitro and in vivo,which may represent a promising strategy for HCC therapy. | Ren-Yu Zhang Ze-Kun Liu Ding Wei Yu-Le Yong Peng Lin Hao Li Man Liu Nai-Shan Zheng Ke Liu Cai-Xia Hu Xiao-Zhen Yang Zhi-Nan Chen Huijie Bian | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 3 |
| 8 | New insights into follicular helper T cell response and regulation in autoimmune pathogenesis显示文摘Follicular helper T (Tfh) cells play a key role in driving B cell activation and differentiation during germinal center reactions in immune responses and autoimmune development, and these cells are characterized by high expression of CXCR5, PD1, ICOS, IL-21 and BCL6. Increasing evidence indicates that the functional dysregulation of Tfh cells contributes to the pathogenesis of autoimmune diseases, including primary Sjögren’s syndrome (pSS), which is a common autoimmune disease characterized by lymphocytic infiltration and tissue inflammation in salivary glands (SGs) and lacrimal glands that leads to dry mouth and dry eyes.1 Here, we provide a brief commentary on recent advances in understanding the Tfh cell response with a focus on new insights into Tfh cell regulation and therapeutic implications in autoimmune diseases. | Fan Xiao Man Han Ke Rui Xiangyan Ai Jie Tian Wen Zhang Futao Zhao Yan Zhao Quan Jiang Liwei Lu | 2021 | Cellular & Molecular Immunology2021，18，6： | 3 |
| 9 | Microstructural Evolution and Performance of In-situ Ag-Ni Composite after Solidification under Electromagnetic Stirring and Deformation显示文摘The effect of electromagnetic stirring(EMS)on microstructure and performance of Ag-8 mass%Ni composite was investigated under both solidified and deformed conditions.Without EMS,the Ag matrix formed short,thick dendrites in the ingot;whereas with EMS,dendrites were long and slim.Ni phase mainly formed particles or ribbons,distributed along boundaries between dendrite arms.Cold drawing of the solidified Ag-Ni ingots,both with and without EMS,produced high strength in-situ metal-matrix composite(MMC)consisting of Ag matrix reinforced by Ni ribbons.EMS improved the ductility of the composite,consequently enhancing its drawability and strength.EMS also increased the electrical conductivity in both solidified ingots and deformed in-situ composite wires.In both cases,hardness and tensile strength remained high.A model based on a combination of the modified linear rule of mixtures and the Hall-Petch relationship was used to rationalize the tensile strength and hardness with respect to its fabrication parameters and the microstructure of Ag-Ni in-situ composite. | Lin ZHANG Ke HAN Tian-nan MAN En-gang WANG Xiao-wei ZUO | 2016 | Journal of Iron and Steel Research(International)2016，23，7： | 2 |
| 10 | Synthesis and characterization of novel star-shaped pyridine cored compounds with alternating carbazole and triphenylamine moieties显示文摘Amorphous 2,4,6-trissubstituted pyridines containing three peripheral carbazole or two triphenylarnine and one carbazole moieties,respectively,have been synthesized and characterized.The properties of the compounds are investigated by UV-vis absorption,photoluminescence spectroscopy,thermal analysis as well as cyclic voltammetry.The results show that the compounds have high thermal stability,emit blue light.Also,the compounds possess the HOMO and LUMO energy levels comparable to those of NPB.The effects of different substituents on the electronic properties of the materials have been discussed. | Ji Bing Yuan Zhi Guo Zhang Louis Man Leung Ke Li Zhang | 2008 | Chinese Chemical Letters2008，19，6： | 2 |
| 11 | Amination of electron deficient aryl chlorides promoted by nano sized Mg(OH)_2 under transition metals free condition显示文摘这份报纸论述缺乏的芳基氯化物由 Mg 支持了的电子的胺化作用反应(哦) 2 没有转变金属。仅仅用 stoichiometric, nano 的数量缩放 Mg (哦) 2,对中等孤立的收益好能在 150 在 24 h 以后在 N-methylpyrrolidone (NMP ) | Zhi Hao Cui Zi Hui Meng Yan Qing Mi Peng Wang Qi Wang Lin Man Zhao Ke Jian Cui Fang Yu Zhi Bin Xu | 2012 | Chinese Chemical Letters2012，23，2： | 2 |
| 12 | Scoring the collective effects of SNPs: association of minor alleles with complex traits in model organisms显示文摘It has long been assumed that most parts of a genome and most genetic variations or SNPs are non-functional with regard to reproductive fitness.However,the collective effects of SNPs have yet to be examined by experimental science.We here developed a novel approach to examine the relationship between traits and the total amount of SNPs in panels of genetic reference populations.We identified the minor alleles(MAs)in each panel and the MA content(MAC)that each inbred strain carried for a set of SNPs with genotypes determined in these panels.MAC was nearly linearly linked to quantitative variations in numerous traits in model organisms,including life span,tumor susceptibility,learning and memory,sensitivity to alcohol and anti-psychotic drugs,and two correlated traits poor reproductive fitness and strong immunity.These results suggest that the collective effects of SNPs are functional and do affect reproductive fitness. | YUAN DeJian ZHU ZuoBin TAN XiaoHua LIANG Jie ZENG Chen ZHANG JieGen CHEN Jun MA Long DOGAN Ayca BROCKMANN Gudrun GOLDMANN Oliver MEDINA Eva RICE Amanda D. MOYER Richard W. MAN Xian YI Ke LI YanKe LU Qing HUANG YiMin HUANG Shi | 2014 | Science China(Life Sciences)2014，57，9： | 2 |
| 13 | Effects of Oxygen Partial Pressure on Resistive Switching Characteristics of ZnO Thin Films by DC Reactive Magnetron Sputtering 显示文摘 | Ji Z Man Q Ke W | 2010 | Solid State Communications2010，150，： | 1 |
| 14 | A case of human infection with a novel Babesia species in China显示文摘Background:Babesiosis is an uncommon but emerging tick-borne disease caused by the genus Babesia.In this case study,we report a case of human infection with a novel Babesia sp.in China.Findings:The patient in question had been suffering from repetitive occurrences of mild fever of unknown origin and fatigue for 10 years.Ring forms,tetrads,and one or two dots of chromatin or trophozoite-like organisms were observed in the patient’s thin blood smears and bone marrow smears.Using a confocal laser-scanning microscope,it was observed that the patient’s serum had reactivity with the surface proteins of the B.microti strain.Electron microscopy revealed oval red blood cells with 1~2μm of knob protrusions in the cellular membrane.The results of the Babesia-specific nested PCR assay for 18S rRNA confirmed the presence of Babesia infection.The construction of a phylogenetic relationship showed clustering with B.microti and B.duncani,which was identified as a novel Babesia species and named as Babesia sp.XXB/HangZhou.Azithromycin,doxycycline,and moxifloxacin hydrochloride were shown to relieve symptoms but were not as effective after continuous usage.After atovaquone(Mepron®)administration,the patient recovered from fever and tested negative for detection of Babesia-specific genes.Conclusion:Babesia sp.XXB/HangZhou is a novel Babesia species,which causes mild babesiosis in an immunocompetent patient. | Su-Qin Man Ke Qiao Jie Cui Meng Feng Yong-Feng Fu Xun-Jia Cheng | 2016 | Infectious Diseases of Poverty2016，5，1： | 1 |
| 15 | Complementary doping of van der Waals materials through controlled intercalation for monolithically integrated electronics显示文摘Doping control has been a key challenge for electronic applications of van der Waals materials.Here,we demonstrate complementary doping of black phosphorus using controlled ionic intercalation to achieve monolithic building elements.We characterize the anisotropic electrical transport as a function of ion concentrations and report a widely tunable resistivity up to three orders of magnitude with characteristic concentration dependence corresponding to phase transitions during intercalation.As a further step,we develop both p-type and n-type field effect transistors as well as electrical diodes with high device stability and performance.In addition,enhanced charge mobility from 380 to 820 cm^2/(V·s)with the intercalation process is observed and explained as the suppressed neutral impurity scattering based on our ab initio calculations.Our study provides a unique approach to atomically control the electrical properties of van der Waals materials,and may open up new opportunities in developing advanced electronics and physics platforms. | Ming Ke Huu Duy Nguyen Hang Fan Man Li Huan Wu Yongjie Hu | 2020 | Nano Research2020，13，5： | 1 |
| 16 | Novel Efficient Blue Fluorophors with Small Singlet‐Triplet Splitting: Hosts for Highly Efficient Fluorescence and Phosphorescence Hybrid WOLEDs with Simplified Structure显示文摘 | Cai‐Jun Zheng Jing Wang Jun Ye Ming‐Fai Lo Xiao‐Ke Liu Man‐Keung Fung Xiao‐Hong Zhang Chun‐Sing Lee | 2013 | Adv Mater2013，，15： | 1 |
| 17 | PCNA : structure, functions and interactions 显示文摘 | Ke|man Z | 1997 | Oncogene1997，14，6： | 1 |
| 18 | Risk factor for glioma in adults: a casecontrol study in northeast china显示文摘 | Hu J Johnson KE Man Y | 1998 | Cancer Defect Prey1998，22，2： | 1 |
| 19 | Complementary and alternative medicine use among cancer survivors:a population-based study 显示文摘 | Man JJ Palmer CS Healy KE | 2011 | J Cancer Surviv2011，5，1： | 1 |
| 20 | FSAP aggravated endothelial dysfunction and neurological deficits in acute ischemic stroke due to large vessel occlusion显示文摘Revascularization and angiogenesis,as substrates of sustained collateral circulation,play a crucial role in determining the severity and clinical outcome of acute ischemic stroke(AIS)due to large vessel occlusion(LVO).Developing an adjunct biomarker to help identify and monitor collateral status would aid stroke diagnosis and prognosis.To screen the potential biomarkers,proteomic analysis was performed in this study to identify those distinct plasma protein profiles in AIS due to LVO with different collateral status.Interestingly,we found that levels of Plasma Factor VII Activating Protease(FSAP)significantly increased in those AIS patients with poor collaterals,and were correlated with worse neurological outcome.Furtherly,both in vitro and in vivo models of ischemic stroke were used to explore pathological mechanisms of FSAP in endothelial dysfunction.We demonstrated that the FSAP inhibitor,high-molecular-weight hyaluronan(HMW-HA),enhanced the pro-angiogenic vascular factors,improved the integrity of brain blood barrier,and promoted newly formed cerebral microvessels in the ischemic penumbra,consequently improving neurological function.To elucidate the pathways that might contribute to revascularization during LVO,we applied transcriptomic analysis via unbiased RNA sequencing and showed that Wnt signaling was highly involved in FSAP mediated endothelial dysfunction.Notably,inhibition of Wnt5a largely reversed the protective effects from HMW-HA treatment,implying that FSAP might aggravate endothelial dysfunction and neurological deficits by regulating Wnt5a signaling.Therefore,FSAP may represent a potential biomarker for collateral status after LVO and a promising therapeutic target to be explored in the treatment of stroke. | Dai-Shi Tian Chuan Qin Luo-Qi Zhou Sheng Yang Man Chen Jun Xiao Ke Shang Dale BBosco Long-Jun Wu Wei Wang | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 1 |
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